Research article Special Issues

Effect and mechanism of long non-coding RNA ZEB2-AS1 in the occurrence and development of colon cancer

  • Received: 20 February 2019 Accepted: 10 July 2019 Published: 06 September 2019
  • ObjectiveClarify the expression changes, biological functions and related mechanisms of long non-coding RNA (lncRNA) ZEB2-AS1 in colon cancer tissues. Methods The expression levels of ZEB2-AS1 in colon cancer tissues and adjacent tissues were detected by qRT-PCR and in situ hybridization methods. Cell biology experiments were performed to detect the proliferation, migration and apoptosis of colon cancer cells when the level of ZEB2-AS1 was overexpression or silencing. Then, Western blot was performed to analyze the effect of ZEB2-AS1 on the expression levels of β-catenin protein and related genes in the signal pathway. Results We found that the expression level of ZEB2-AS1 in colon cancer tissues was significantly up-regulated compared with that in adjacent normal tissues. In colon cancer cell line of HCT8, overexpression of ZEB2-AS1 could promote cell proliferation and migration, while silencing ZEB2-AS1 would enhance cell apoptosis and inhibit proliferation. Study on the mechanism of ZEB2-AS1 showed that it could promote the expression of β-catenin, activate downstream genes to be transcribed and promote the occurrence and development of tumors. Conclusion ZEB2-AS1 could promote colon cancer cell proliferation and inhibit apoptosis to promote the progression of colon cancer by upregulating the expression of β-catenin protein. ZEB2-AS1 may be a useful new target for treating colon cancer patients.

    Citation: Xu Guo, Yuanming Jing, Haizhou Lou, Qiaonv Lou. Effect and mechanism of long non-coding RNA ZEB2-AS1 in the occurrence and development of colon cancer[J]. Mathematical Biosciences and Engineering, 2019, 16(6): 8109-8120. doi: 10.3934/mbe.2019408

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  • ObjectiveClarify the expression changes, biological functions and related mechanisms of long non-coding RNA (lncRNA) ZEB2-AS1 in colon cancer tissues. Methods The expression levels of ZEB2-AS1 in colon cancer tissues and adjacent tissues were detected by qRT-PCR and in situ hybridization methods. Cell biology experiments were performed to detect the proliferation, migration and apoptosis of colon cancer cells when the level of ZEB2-AS1 was overexpression or silencing. Then, Western blot was performed to analyze the effect of ZEB2-AS1 on the expression levels of β-catenin protein and related genes in the signal pathway. Results We found that the expression level of ZEB2-AS1 in colon cancer tissues was significantly up-regulated compared with that in adjacent normal tissues. In colon cancer cell line of HCT8, overexpression of ZEB2-AS1 could promote cell proliferation and migration, while silencing ZEB2-AS1 would enhance cell apoptosis and inhibit proliferation. Study on the mechanism of ZEB2-AS1 showed that it could promote the expression of β-catenin, activate downstream genes to be transcribed and promote the occurrence and development of tumors. Conclusion ZEB2-AS1 could promote colon cancer cell proliferation and inhibit apoptosis to promote the progression of colon cancer by upregulating the expression of β-catenin protein. ZEB2-AS1 may be a useful new target for treating colon cancer patients.


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    [1] Y. Yin, J. Zhong, S. W. Li, et al., TRIM11, a direct target of miR-24-3p, promotes cell proliferation and inhibits apoptosis in colon cancer, Oncotarget,7 (2016), 86755-86765.
    [2] K. Liu, H. Yao, S. Lei, et al., The miR-124-p63 feedback loop modulates colorectal cancer growth, Oncotarget,8 (2017), 29101-29115.
    [3] M. Yue, J. L. Charles Richard and Y. Ogawa, Dynamic interplay and function of multiple noncoding genes governing X chromosome inactivation, Biochim. Biophys. Acta,1859 (2016), 112-120.
    [4] A. A. Butler, W. M. Webb and F. D. Lubin, Regulatory RNAs and control of epigenetic mechanisms: expectations for cognition and cognitive dysfunction, Epigenomics,8 (2016), 135-151.
    [5] C. Kanduri, Long noncoding RNAs: Lessons from genomic imprinting, Biochim. Biophys. Acta,1859 (2016), 102-111.
    [6] C. Tordonato, P. P. Di Fiore and F. Nicassio, The role of non-coding RNAs in the regulation of stem cells and progenitors in the normal mammary gland and in breast tumors, Front Genet.,6 (2015), 72.
    [7] J. R. Prensner, M. K. Iyer, O. A. Balbin, et al., Transcriptome sequencing across a prostate cancer cohort identifies PCAT-1, an unannotated lincRNA implicated in disease progression, Nat. Biotechnol.,29 (2011), 742-749.
    [8] W. Li, J. Zheng, J. Deng, et al., Increased levels of the long intergenic non-protein coding RNA POU3F3 promote DNA methylation in esophageal squamous cell carcinoma cells, Gastroenterology,146 (2014), 1714-1726.e1715.
    [9] R. Chen, G. Wang, Y. Zheng, et al., Long non-coding RNAs in osteosarcoma, Oncotarget,8 (2017), 20462-20475.
    [10] Z. Yang, X. Li, Y. Yang, et al., Long noncoding RNAs in the progression, metastasis, and prognosis of osteosarcoma, Cell Death Dis.,7 (2016), e2389.
    [11] K. P. Zhu, X. L. Ma and C. L. Zhang, LncRNA ODRUL Contributes to Osteosarcoma Progression through the miR-3182/MMP2 Axis, Mol. Ther.,25 (2017), 2383-2393.
    [12] B. Wang, Y. Su, Q. Yang, et al., Overexpression of Long Non-Coding RNA HOTAIR Promotes Tumor Growth and Metastasis in Human Osteosarcoma, Mol. Cells,38 (2015), 432-440.
    [13] Y. Guo, Y. Hu, M. Hu, et al., Long non-coding RNA ZEB2-AS1 promotes proliferation and inhibits apoptosis in human lung cancer cells, Oncol. Lett.,15 (2018), 5220-5226.
    [14] H. Gao, N. Gong, Z. Ma, et al., LncRNA ZEB2-AS1 promotes pancreatic cancer cell growth and invasion through regulating the miR-204/HMGB1 axis, Int. J. Biol. Macromol.,116 (2018), 545-551.
    [15] P. Diao, H. Ge, Y. Song, et al., Overexpression of ZEB2-AS1 promotes epithelial-to-mesenchymal transition and metastasis by stabilizing ZEB2 mRNA in head neck squamous cell carcinoma, J. Cell. Mol. Med.,23 (2019), 4269-4280.
    [16] X. Shi, J. Li, L. Ma, et al., Overexpression of ZEB2-AS1 lncRNA is associated with poor clinical outcomes in acute myeloid leukemia, Oncol. Lett.,17 (2019), 4935-4947.
    [17] C. Xu, H. Cui, H. Li, et al., Long non-coding RNA ZEB2-AS1 expression is associated with disease progression and predicts outcome in gastric cancer patients, J. Buon.,24 (2019), 663-671.
    [18] S. Xu, D. Kong, Q. Chen, et al., Oncogenic long noncoding RNA landscape in breast cancer, Mol. Cancer,16 (2017), 129.
    [19] G. Zhang, H. Li, R. Sun, et al., Long non-coding RNA ZEB2-AS1 promotes the proliferation, metastasis and epithelial mesenchymal transition in triple-negative breast cancer by epigenetically activating ZEB2, J. Cell. Mol. Med.,23 (2019), 3271-3279.
    [20] F. Wang, W. Zhu, R. Yang, et al., LncRNA ZEB2-AS1 contributes to the tumorigenesis of gastric cancer via activating the Wnt/beta-catenin pathway, Mol. Cell. Biochem.,456 (2019), 73-83.
    [21] C. He, W. Yang, J. Yang, et al., Long noncoding RNA MEG3 negatively regulates proliferation and angiogenesis in vascular endothelial cells, DNA Cell Biol.,36 (2017), 475-481.
    [22] L. E. Dow, K. P. O'Rourke, J. Simon, et al., APC restoration promotes cellular differentiation and reestablishes crypt homeostasis in colorectal cancer, Cell,161 (2015), 1539-1552.
    [23] J. N. Anastas and R. T. Moon, WNT signalling pathways as therapeutic targets in cancer, Nat. Rev. Cancer,13 (2013), 11-26.
    [24] J. Li, B. Yu, P. Deng, et al., KDM3 epigenetically controls tumorigenic potentials of human colorectal cancer stem cells through Wnt/beta-catenin signalling, Nat. Commun.,8 (2017), 15146.
    [25] P. Zhu, Y. Wang, G. Huang, et al., lnc-beta-Catm elicits EZH2-dependent beta-catenin stabilization and sustains liver CSC self-renewal, Nat. Struct. Mol. Biol.,23 (2016), 631-639.
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