Citation: Cynthia M. LeRouge, Donghua Tao, Jennifer Ohs, Helen W. Lach, Keri Jupka, Ricardo Wray. Challenges and Opportunities with Empowering Baby Boomers for Personal Health Information Management Using Consumer Health Information Technologies: an Ecological Perspective[J]. AIMS Public Health, 2014, 1(3): 160-181. doi: 10.3934/publichealth.2014.3.160
[1] | Sébastien Bernacchi, Simon Rittmann, Arne H. Seifert, Alexander Krajete, Christoph Herwig . Experimental methods for screening parameters influencing the growth to product yield (Y(x/CH4)) of a biological methane production (BMP) process performed with Methanothermobacter marburgensis. AIMS Bioengineering, 2014, 1(2): 72-87. doi: 10.3934/bioeng.2014.2.72 |
[2] | Karthikeyan D. Ramachandriya, Dimple K. Kundiyana, Ashokkumar M. Sharma, Ajay Kumar, Hasan K. Atiyeh, Raymond L. Huhnke, Mark R. Wilkins . Critical factors affecting the integration of biomass gasification and syngas fermentation technology. AIMS Bioengineering, 2016, 3(2): 188-210. doi: 10.3934/bioeng.2016.2.188 |
[3] | Adam B Fisher, Stephen S Fong . Lignin biodegradation and industrial implications. AIMS Bioengineering, 2014, 1(2): 92-112. doi: 10.3934/bioeng.2014.2.92 |
[4] | Velislava N Lyubenova, Maya N Ignatova . On-line estimation of physiological states for monitoring and control of bioprocesses. AIMS Bioengineering, 2017, 4(1): 93-112. doi: 10.3934/bioeng.2017.1.93 |
[5] | Daniel Borchert, Diego A. Suarez-Zuluaga, Yvonne E. Thomassen, Christoph Herwig . Risk assessment and integrated process modeling–an improved QbD approach for the development of the bioprocess control strategy. AIMS Bioengineering, 2020, 7(4): 254-271. doi: 10.3934/bioeng.2020022 |
[6] | Vered Tzin, Ilana Rogachev, Sagit Meir, Michal Moyal Ben Zvi, Tania Masci, Alexander Vainstein, Asaph Aharoni, Gad Galili . Altered Levels of Aroma and Volatiles by Metabolic Engineering of Shikimate Pathway Genes in Tomato Fruits. AIMS Bioengineering, 2015, 2(2): 75-92. doi: 10.3934/bioeng.2015.2.75 |
[7] | Islam Uddin, Salman A. AlQahtani, Sumaiya Noor, Salman Khan . Deep-m6Am: a deep learning model for identifying N6, 2′-O-Dimethyladenosine (m6Am) sites using hybrid features. AIMS Bioengineering, 2025, 12(1): 145-161. doi: 10.3934/bioeng.2025006 |
[8] | Urooj Ainuddin, Maria Waqas . Finite state machine and Markovian equivalents of the lac Operon in E. coli bacterium. AIMS Bioengineering, 2022, 9(4): 400-419. doi: 10.3934/bioeng.2022029 |
[9] | Zongyuan Zhu, Rachael Simister, Susannah Bird, Simon J. McQueen-Mason, Leonardo D. Gomez, Duncan J. Macquarrie . Microwave assisted acid and alkali pretreatment of Miscanthus biomass for biorefineries. AIMS Bioengineering, 2015, 2(4): 449-468. doi: 10.3934/bioeng.2015.4.449 |
[10] | Maria Waqas, Urooj Ainuddin, Umar Iftikhar . An analog electronic circuit model for cAMP-dependent pathway—towards creation of Silicon life. AIMS Bioengineering, 2022, 9(2): 145-162. doi: 10.3934/bioeng.2022011 |
Abbreviations list:
CO2in = specific CO2 flow rate entering the reactor [mol L-1h-1]; CSTR = continuous stirred tank reactor; CUR = carbon dioxide uptake rate [mol L-1h-1]; Dmed = specific rate of medium added to the reactor [Lmedium L-1reactor volume h-1]; Dout = dilution rate [Lleaving the system L-1reactor volume h-1]; DWER = specific rate of water generated by the reaction [L L-1reactor volume h-1]; Effferm = efficiency of reactor considering compression of Comp1, QH2 and QCH4; Efftot1 = total efficiency of the system considering 2-step compression, stirring, QH2 and QCH4; Efftot2 = total efficiency of the system considering 2-step compression, stirring, QH2 , QCH4 and Qheat; ΔG° = Gibbs free energy; HTR = hydrogen transfer rate [mol L-1h-1]; HUR = hydrogen uptake rate [mol L-1h-1]; H2Liq = equilibrium concentration of H2 in the liquid phase [mol L-1]; Hu = net calorific value [MJ kg-1]; Ks = the specific substrate concentration at which the reactor rate is half of the maximum rate; MER = methane evolution rate [mol L-1h-1]; QCH4 = heat content of CH4 generated in the process [kW L-1]; QH2 = heat content of H2 provided to the process [kW L-1]; Qheat = heat generated during the compression process [kW L-1]; VLE = vapor liqui.equilibrium; vvh = volume of gas per volume of liquid and hour [L L-1h-1]; WComp1 = work used for compression at compressor 1 pressurizing the core process [kW L-1]; WComp2= work used for compression at compressor 2 pressurizing the purification unit [kW L-1]; WStirrer= work used for stirring [kW L-1]; Y x/s = biomass yield on substrate [C-mol mol-1].
The chemical storage of energy in the form of CH4 generated from renewable resources transforming H2 to CH4 by CO2 fixation is a topic which emerged as the storage of H2 at an appropriate energy density is difficult [1,30]. The here introduced technology enables to gain an energy carrier with a high energy content which can be introduced in the existing natural gas infrastructures. In addition it proposes a biological alternative to perform chemical methanation reactions. The so-called Sabatier reaction is performed at high temperatures and pressures therefore impacting process economy and carbon balance. Biology operating at milder conditions, the bioprocessing route might be more attractive.
Hydrogenotrophic methanogens are Archaea microorganisms which can use hydrogen for reducing i.e. formate, methanol or carbon dioxide to methane [2,3]. Methanogens metabolizing CO2 hold a great potential for the development of biological gas conversion processes. To achieve CO2 neutrality, a bioprocess can be designed where such a microorganism catalyzes the gaseous transformation of H2 and CO2 to CH4 and H2O The chemical storage of electrical energy in the form of CH4 with the intermediate step of H2 production by electrolysis (H2O→H2+0.5O2
The core this process is the reaction described in the following stoichiometri.equation (1). The selectivity towards CH4 for the used microorganism, able to metabolize 95% of the gaseous substrate to CH4 and only 5% to biomass, suggested an efficient overall process [6,27,33]
[CO2, Liq + 4 H2, Liq → CH4, gas + 2 H2OLiq]; | (1) |
The process simulation software Aspen Plus [4] was used to analyze the process in terms of mass and energy balances. Aspen Plus is a simulation tool regularly used in chemical process engineering [5]. Although applications to bioprocesses are rare, it is also a suitable tool for bioprocess balancing. In line with the current contribution the possibility to perform mass and energy balance calculations as well as process integration studies was reported for other bioprocesses such as the production of bio-hydrogen [7,8,9]. Another study described the economic comparison of ethanol production by Z. mobilis and Saccharomyces and was performed in Aspen Plus [10]. But, so far, no publication concerning process evaluation with Aspen Plus exists for a biological methanogenesis process.
In this work, the focus was not the simulation of physiologic aspects such as biomass formation. As a preliminary input to this contribution, the physiology was studied experimentally at lab-scale in order to retrieve the production rates and scalable parameters that were used for the kinetic model of the reaction unit [6,27,33].
The aim of the simulation was to identify key process related factors and their influence on the overall process efficiency. The experimental kinetic model ensures the conservative basis of the simulation as it limits speed of reaction and CH4 productivity. At large scale, economic, energy and safety requirements have to be fulfilled. Therefore this work focuses on a basic understanding of the proposed process and how parameters such as operating pressure, dilution of reactant gases or stripping and scrubbing of media components influence the process performance.
The following section describes the proposed process, the general assumptions, the reaction model and the parameters used within the simulation. The integrated process implemented in the simulation tool Aspen Plus includes: mixing of reactants prior to the reactor entry, the reaction step and the purification of the produced gas to a pipeline quality.
Based on the developed simulation model, the overall energy efficiency of the integrated process is then analyzed.
The proposed overall process is shown in Figure 1. The reactant gases H2 and CO2 are mixed in the unit “MIXINGAS” using a mixer model. The resulting stream is compressed to the desired pressure in the reactor block by an isentropic compressor “COMPR1”. Compressed gas is adjusted to 65 ℃ by the unit “HEAT1”. In the equilibrium reactor unit “REQUIL” the gas is mixed with medium and the pH of reaction is adjusted with NaOH addition. Finally, in the reactor block “REQUIL” incoming gases H2 and CO2 are converted to CH4, H2O and biomass. The reactor used i.experiments was a continuous stirred tank bioreactor replaced in the simulation by an equilibrium reactor model, in which the kinetic model was implemented. The reactor model allows a flexible implementation of rates, conversion and selectivity of reaction based on experimental data as well as the simultaneous calculation of vapor and liqui.equilibrium.
As the microorganism acts as a biocatalyst it is of interest to limit biomass washout from the reactor as well as the effluent rate. Strategies for reducing dilution rate were investigated and are further explained in the results section. A cell retention system mimicking a tangential flow filtration step (units “MIX1”, “CELLRET” and “SEP1”) was implemented in the flow-sheet.
The gas phase stream leaving the reactor “REQUIL” contains the produced CH4 and H2O as well as excess H2, H2S, NH3 and CO2. After leaving the reactor, the gas is cooled at 25 ℃ in the flash unit “FCSR25”, which removes condensate assuming thermodynami.equilibrium between liquid and gas phase. Subsequently, the dried gas is compressed in unit “COMPR2” to a pressure of 21 bar, assumed to be necessary for operating the gas permeation unit. The compressed gas finally enters the gas permeation unit “MEMBRANE”, where CH4 is separated from H2 and CO2. Gas permeation unit “MEMBRANE” at the moment is implemented via a simple component split unit. The permeating H2 and CO2 are recycled and mixed to the fresh H2 and CO2 in the upstream mixer unit “MIXINGAS”. All heat streams generated in the core reactor unit or at the different compressors are collected and summed up in the unit “GMIX”.
Design specifications, working similar to a feedback controller, were used in the simulation model, to control pH as well as to adjust the ratio between H2 and CO2 to 4:1 at the reactor inlet. These parameters where chosen in order to match physiologic optimum predetermined i.earlier studies [6,27,33].
The reaction kinetic model is based on the following assumptions:
a) Production of CH4 depends, according to (1) on CO2 and H2 dissolved in the liquid phase. As CO2 solubility is much higher than H2, only H2 concentration in the liquid phase (H2Liq) is regarded as limiting for the kinetic model.
c) Equilibrium concentration of H2Liq happens according to Henry equation and hence changes with pH, temperature and pressure.
d) Aspen Plus assumes ideal mixing in the reactor unit. Methane productivity was limited by the kinetic model based on experimentally verified values achieved in a lab-scale bioreactor [27,33].
Calculations performed within this paper are based on the following parameters, assumptions and simplifications:
• Electrolyte-NRTL activity coefficient model [11], ELECNRTL, was used for the calculation of activity coefficients in the liquid phase, capable to consider the electrolyte character of the reaction medium. Vapor phase is described via the Redlich-Kwong equation of state [4]. Gas solubility calculations are based on Henry’s law.
• For methanogenesis reaction (1), the equilibrium reactor model was chosen as explained in section 2.1.1. Methane evolution rate (MER), (2) was implemented with a calculator block using the kinetic model.
• Biological methanogenesis is a bioprocess performing tendentiously under gas transfer limitation. Biomass is seen as a byproduct of reaction. Biomass is assumed, based on experimental results for the simulated range of parameters to be always sufficient to turn over all the dissolved H2 [6,27,33].
• All side reactions to (1), except biomass formation are neglected.
• Equilibrium was assumed for gas absorption and desorption.
• Reaction temperature was set to 65 ℃.
• A working pH of 7 was applied except if not specified differently
• CO2 and H2 inlet flow rates were held at a ratio of 1:4 according to reaction (1) stoichiometry in the stream MIXEDGAS by using a design specification.
• The total inlet flow-rate was 0.73 L L-1min-1 for all calculations, if not indicated otherwise.
An overview of further process parameters and their variation range is given in Table 1. The default media composition implemented has been extracted from previously published results [6,27,33].
Unit | Type | Temperature | Pressure |
Reactor | equilibrium | 65℃ | 1 bar-11 bar |
Compressor 1 | isentropic | 65℃ | 1 bar-11 bar |
Compressor 2 | isentropic | 65℃ | 21 bar |
The kinetic of this bioprocess is known to be dependent on many chemical as well as biological parameters and so far no kinetic model for MER is available. The main reason is the gas limited character deriving from the poor solubility of H2 at ambient pressure. The process operates tendentiously under the limitation of a gas to liquid H2 mass transfer [2,6,12,27,33]. One reason is that most bioreactors available, despite high kLax values, are limited in terms of operating pressures, which would guarantee improved H2 solubility.
Due to the complexity of the biological system, a simple first order enzymatic reaction kineti.equation was used as model for obtaining mass and energy balances [31]. This simplified approach, can account for the selectivity of reaction towards biomass. In fact, the cell metabolism was implemented assuming no side reactions except for biomass formation. In this approach 95% of the C is available for CH4 production and so the maximum MER cannot exceed 95% of the CO2 inlet rate. The remaining 5% of carbon is attributed to biomass formation using the yield Yx/s = 0.05 [C-mol mol-1] which was reported constant under gas limited conditions [6,27,33]. The MER model is presented in (2) and (3). The gas to liquid transfer is based on equilibrium calculations following Henryxs law and affected by pH, temperature and pressure [13]. Aspen Plus calculates H2Liq also as a function of the system pressure (Figure 2). Therefore, pressure dependence emerged in MER calculations but the CO2 inlet determines the maximum MER and was set according to experiments.
MER=(1−Yx/s)∙CO2[molL−1h−1]∙H2Liq[molL−1]KM[molL−1]+H2Liq[molL−1] |
(2) |
KM=0.00769[molL−1] |
(3) |
In thi.empiric model the parameter KM is a constant determined by fitting experimental and simulated MERs obtained at different H2Liq concentrations. H2Liq was varied experimentally by increasing operating pressure for a fixed CO2 inlet. This allowed an increase in MERs but never reaching the total conversion of CO2 and H2 [2,6,27,33].
The model was validated for various cases by comparing MERs obtained by the model with values obtained experimentally (Figure 3). It can be clearly seen that MERs calculated with the used reaction model closely match with experimentally obtained MERs. The fit between reaction model and experimental results was within 5% error. Hence, all MER values from simulation are considered to be close estimates. Detailed information on the method for experimentally obtained data as well as maximum specific methane productivity per gram of biomass is available in literature [6,27,33].
In addition, verification of the thermodynamic aspects of simulation was investigated. To prove, that the obtained heat of reaction is calculated correctly, ΔG° of reaction was examined. In literature a ΔG° value of -130.7 kJ mol-1 CH4 is reported at 298.15 K [19]. A slightly elevated ΔG° was expected, as the reaction temperature is higher than in Dolfing. approach [19]. Using ΔH and ΔS obtained with Aspen Plus, a ΔG = -126.6 kJ mol-1 was determined at ambient pressure and 338.15 K. Therefore, the simulation is considered thermodynamically sound.
The impact of pressure on the system is important for two reasons. First, the effect of pressure on gas solubility is well known and contributes to increase the amount of dissolved gas available for the microorganisms. In this simulation the model was used to extrapolate slightly higher MERs at higher pressures but always being limited by the kinetic model as high-pressure experiments were not accessible with the experimental setup. Secondly, for evaluating compression duty for pure and diluted reactant gas mixtures and how process efficiency is influenced by gas feed carrying different amounts of inert gas.
During manual confirmation of compressor results (data not shown) some discrepancies between manually calculated and results obtained by simulation were found. However, in the overall process efficiency this discrepancy is minimized to some percent. In addition, Aspen Plus was overestimating the power of compression. Hence, only the Aspen Plus calculations are used for the energy balance and further guarantee the conservative basis applied in this bioprocess efficiency simulation.
According to the aims of this study the energy balance of the process was investigated and calculated based on values obtained with the process simulation.
The theoretical energy potential can be calculated easily based on the net calorific value (Hu):
Hu, CH4= 50.013MJ kg-1[20]
Hu, H2= 119.972MJ kg-1[20]
Based on equation (1) 1 mol CH4 (0.2233 kWh) can be produced out of 4 mol H2 (0.268 kWh). The maximum stoichiometri.efficiency calculated by 0.223 kWh / 0.268 kWh= 83.2%. Considering that about 5% of carbon is used for cell growth the maximum efficiency can be calculated by (0.223 kWh•0.95) / 0.268 kWh = 79.2%.
From this theoretical efficiency the following values have to be subtracted:
Energy input for stirring: Wstirrer
Energy input for compression: WComp1+WComp2
A value of around 5 kW m-³ of non-aerated reactor volume, commonly used in industrial process technology, is taken as stirring energy input [29].
An additional, however not directly usable heat stream is the heat of compression. Qheat generated by the cooling of the two compressors.
The heat of reaction from biological methanogenesis can be significant and the reactor vessels would need to be cooled. However, reaction heat was not taken into account for the efficiency calculations. The temperature level of this heat stream corresponds, at the maximum, to the reaction temperature (65℃) and may only be recuperated locally.
To get a ranged estimate for the accessible energy (in form of accessible heat or CH4 product) both calculations with and without including Qheat are shown. The results give the higher and the lower bound of the process efficiency regardless of the real thermodynamic of transformation.
Hence, the overall efficiency of the system was obtained using the following correlations:
![]() |
(4) |
![]() |
(5) |
In contrast, the core reaction efficiency (Effferm) only includes H2 input, compressor 1 duty and CH4 output.
![]() |
(6) |
Providing nutrients to avoid limitation is a crucial task in bioprocess development and was found to be of high importance for biological methanogenesis [9]. Furthermore, accurate calculation of the solubility equilibrium of media components and their becoming in multi component mixtures is important to assist media development for different process conditions. This information can for example be used for elemental balancing of components that are not directly accessible with the available analytics or to predict certain limitations due to changing process conditions. In addition this knowledge can be used for the development of a proper feeding strategy. The capability of Aspen Plus to calculate stripping rates was used here for volatile substrates formed from non-volatile salts contained in the medium. Different process conditions (temperature, pH, and gassing rate) in the bioreactor were evaluated by calculating the involved gas-liqui.equilibrium.
Nitrogen is usually fed to the microorganisms in the form of NH4Cl. In order to check nitrogen losses from the bioreactor medium in the form of NH3, simulations in Aspen Plus were performed for different NH4Cl flow rates as function of the reaction pH at a fix dilution rate. At higher pH value more NH3 is released to the gas phase and lost from the bioreactor media (Figure 4A). However, only a small amount below 0.6% of nitrogen fed to the system in form of NH4Cl is stripped out from the liquid phase as gaseous NH3. The simulated values match data determined by the dissociation equilibrium and Henry’s constant from literature [21,22]. Figure 4A, shows a decreasing percentage of the fed nitrogen stripped to the gas phase at increasing NH4Cl flow rates, with constant pH and gassing flow rate. This i.explained by increased NH4Cl concentration and in absolute more mol stripped from the bioreactor broth but a smaller relative percentage. Due to these small losses of nitrogen, no special attention needs to be paid with respect to NH3 stripping for media development but have to be considered for the development of a downstream purification process.
The process, as described previously, is not limited by CO2 but it however remains an important substrate as it is the sole carbon source in this bioprocess. A closer investigation was therefore required for the CO2 solubility which is dependent on temperature and pressure according to Henry law and on pH due to the dissociation in HCO3- and CO32- [23]. To check the accuracy of solubility calculations, results were compared with literature. Furthermore it was investigated, whether a difference in dissociation and solubility of CO2 can be seen when dissolved in pure H2O or in defined medium. The simulation results were compared with values obtained by using the Henry coefficient kH determined by Harned and Davis, [24] and matched closely (Figure 4B). Furthermore, it was shown that solubility and dissociation in the defined media is close to the CO2 behavior in H2O due to the relatively low overall salt concentration. The microorganism has an optimal growth at pH 7 and thereby far from the pKa of H2CO3 (Figure 4B). The results were used for improving the C-balance of the bioprocess because of different stripping and scrubbing rates depending on the pH value applied.
The medium component Na2S is the sulfur source of this bioprocess and limitation has to be avoided. In aqueous solution it hydrolyzes into NaOH and NaHS. These components immediately dissociate forming HS- (pKa1= 6.9) and S2- (pKa2 > 14) as well as the volatile and toxic component H2S metabolized by the microorganism [26]. At the process pH mainly H2S and HS- are present, and almost no S2-. The solubility equilibrium of sulfur species was simulated in a similar way to CO2. The relationship of S stripping as function of pH, but also as function of gassing rate was here investigated. It was shown in literature that with increasing pH more sulfur stays in the liquid phase, but toxicity increases due to trace element complexation [25]. On the other hand, at lower pH major amount of S is stripped in the gas phase. Results obtained by simulation in H2O show the same relation as with defined media (Figure 4C). At process pH values, most of the added sulfur leaves the system via the gas phase and a change in medium pH has an impact on S availability. Hence, H2S stripping has to be closely regarded and adjusted depending on process conditions.
Analyzing stripping rates behavior for C, N, and S bioprocess substrates demonstrate that Aspen Plus is suitable for predicting pH dependent dissociation and gas solubility in defined media and can serve as a valuable tool for media design and development of gas producing bioprocesses. These results seem trivial considering the thermodynamical basis of Aspen Plus. However, to our knowledge, this has not been reported for complex media composition as it occurs in the anaerobic bioprocess studied in this contribution.
Hydrogen mass transfer is the limitation faced in a biological methanogenesis process. While CO2 is well accessible in the liquid phase due to its good solubility in H2O, H2 is hardly soluble in H2O at ambient pressure. According to Henry’s law, the two parameters temperature and partial pressure influence solubility of gases in the liquid phase. Lowering the reactor temperature would increase the concentration of H2Liq. However, this parameter cannot be significantly lowered because of the microorganism’s temperature dependent growth. Therefore, the effect of increasing pressure was investigated to obtain a better H2 solubility. On the other hand, increasing pressure has considerable impact on compressor duty.
The effect of pressure on reaction efficiency (6) was investigated by comparing energy input to energy output (QCH4) in the range of atmospheric pressure up to 11 bar in the reactor unit. Input energy was determined by summing up QH2 in the reactor and the energy applied for compression (WComp1). The first estimate obtained by simulation shows that higher pressure gives an increased MER compared to the compressor duty (Figure 5A). However, thi.effect is limited by the total amount of gaseous substrate in the feed. At 11 bar about 80% of the total CO2 fed take part in the reaction. Due to the plateau shape of the regression model for estimating MER and a crude linear extrapolation of WComp1 using the simulated values a theoretical optimal pressure of 11 bar is suggested for maximizing the reaction efficiency. The biological effect of high reactor pressures has so far not been published for the specific microorganism used as reference for this bioprocess simulation but was reported already for similar microorganisms [32].
In a real process the sole use of pure gases leads to considerable economic limitations. Hence the impact of using gas mixtures containing increased non-reactive gas proportions was evaluated on the obtained MER, compressor duty and process efficiency. The effects of several emission gases on physiology and productivity was already published [27]. The threshold for acceptable losses in the reaction efficiency due to the use of diluted gas mixtures was set to 20%. As a model for mimicking gas mixtures increasing amounts of the inert gas N2 were added to the reaction mixture of CO2 and H2. While low amounts of N2 show only a small increase in compressor duty, simulation shows that 20% efficiency is lost at about 70% of non-reactive gas. At a simulated gas composition of 70% N2 about 45% of the energy stored in the form of CH4 had to be used for compression (Figure 5B).
This result suggests that the addition of gas not participating to the reaction should be limited to a maximum of 70% to avoid high efficiency losses in the reactor unit. The effect of additional gas on the microorganisms need to be done case by case and can be evaluated in respect to inhibition and toxi.effects according to a published methodology [27]. Purification efficiency for separation of CH4 from non-reactive gas in the permeation unit was not investigated within this work and would depend mostly on the gas composition.
A high feed of H2O poses the risk of cell washout, which works as biocatalyst as well as increasing the amount of effluents. For biological methanogenesis, H2O is fed continuously to the system in the form of medium but is also generated during the production of CH4 (1). To avoid washout of cells and minimize wastewater, feeding of medium has to be minimized. A solution might be to maximize the removal of H2O via the reactor headspace. Therefore, H2O added with the liquid medium feed was set in relation with H2O generated by the reaction through the ratio Dmed/Dout given in %.
The medium feed was varied from 0.005 h-1 to 0.1 h-1 and investigated by process simulation. It was shown, that at a medium dilution rate of about Dmed= 0.005 h-1 only 29% of H2O leaving the system in the liquid phase comes from the feed, while the remaining 71% is H2O produced by the reaction stoichiometry (Figure 6A) finally giving a total dilution rate Dout=0.016 h-1. At higher medium feeds the contribution to total dilution rate Dout rises drastically, showing a large impact on the effluent rate. At standard feeding rate Dmed= 0.05 h-1 H2O formed in the reaction (DWER= 0.011 h-1), contributes only by a small part to the total dilution rate Dout= 0.061 h-1. During experiments a dilution rate (Dmed) of 0.05 h-1 never encountered washout situation at these gassing rates [6,33].
Water leaves the reactor not only in liquid form, but also via the reactor headspace. At standard conditions about 6% of H2O leave via headspace (Figure 6B). So an increase of gas flow rate would increase the amount of H2O removed from the system via the reactor headspace. Since an increase in amount of reacting gases would also increase the amount of stoichiometrically produced H2O, the gas added to the process was increased by addition of inert N2. In Figure 6B the ratio between H2O removed over the headspace and total amount of excess H2O (Dout) was plotted against the concentration of N2 in the gas feed. For these simulation runs no H2O was added by medium feed; all components were added in their native form.
A content of around 80% of inert gas in the gas feed allows the removal of about 50% of H2O generated by the reaction over the headspace (Figure 6 B). In section 3.2 an addition of no more than 70% of inert gas was proposed to keep the energy input for reaction gas compression below 20% of total energy yield. At 70% N2 in the gas feed, only 35% of the formed H2O can be removed via the headspace. Hence, if Dout has to be decreased, a combined approach of minimizing liquid medium feed to 0.005 h-1 and increasing gas flow by addition of 70% of inert gas could be used. The total dilution rate is then lowered from Dout = 0.06 h-1 to Dout = 0.012 h-1. However, lowering dilution rates increase the residence time of media components, which may affect process stability and would need to be investigated separately. In a real application a membrane filtration unit could also be implemented for cell retention. Finally, H2O after condensation might also be considered as an additional byproduct of reaction and a potential renewable H2O source, which could be collected by pervaporation [28].
In the last section, all previous findings were combined to generate an estimation of the overall efficiency for the integrated process. This simulation consists of: the reaction step, the gas purification unit as well as the gas recirculation including all compression steps.
A rise of reactor pressure leads to an increase in MER and a higher efficiency (Section 3.2). The integrated process with unreacted gas recycling also includes gas purification at high pressure. Due to the significant volume contraction of the reaction (1 mol of CO2 and 4 mol of H2 give 1 mol of CH4, (1)) the amount of gas entering is much higher than the amount of gas leaving the reactor. Therefore about 5 times more gas has to be compressed before the bioreactor than at the gas purification step. Thus, the main goal of this section was to find an optimum pressure for the different process steps maximizing the overall process efficiency. It was investigated, whether the increase in MER by increased reactor pressure compensate the higher compression energy.
To investigate the effect of different pressure levels on overall process efficiency, MER and power demand for different process options were calculated considering a maximum pressure of 11 bar in the reactor and 21 bar for the gas purification step. No pressure losses are assumed in the different process steps.
The investigated cases are summarized in Table 2 and Table 3 shows the simulation results in terms of Efftot1(4) and Efftot2 (5) respectively ignoring or considering the heat released by the different process steps.
Case | Pressure at reactor [bar] | Pressure at separation unit [bar] | Efftot1 (Eq. 4) [%] | Efftot2 (Eq. 5) [%] |
A) | 1 | 21 | 67.91 | 73.18 |
B) | 2 | 21 | 70.16 | 75.74 |
C) | 6 | 21 | 68.09 | 73.93 |
D) | 11 | 21 | 66.62 | 74.21 |
Path | Efficiency | Conditions |
Electricity to gas | ||
electricity →H2 | 54-72% | at compression to 200 bar (working pressure of most gas storage plants) |
electricity →CH4 (SNG) | 49-64% | |
electricity →H2 | 57-73% | at compression to 80 bar (feed long distance/transmission pipeline) |
electricity →CH4 (SNG) | 50-64% | |
electricity →H2 | 64-77% | without compression |
electricity →CH4 (SNG) | 51-65% | |
electricity to gas to electricity | ||
electricity →H2→ electricity | 34-44% | at electrification with 60% and compression to 80 bar |
electricity →CH4 (SNG) → electricity | 30-38% | |
electricity to gas to electricity (cogeneration, combined heat and power, CHP) | ||
electricity →H2→ electricity (CHP) | 48 - 62% | at 40% conversion efficiency for electricity, 45% efficiency for heat and compression to 80 bar |
electricity →CH4 (SNG) → electricity (CHP) | 43 - 54% |
Based on the results shown in Figure 7 and the calculated efficiencies it can be seen that an optimum is found at 2 bar for reactor pressure and 21 bar for gas purification. This result is surprising, as the core process efficiency is highest at 11 bar. However, thi.effect can be explained, as previously mentioned, by reaction volume contraction. The first compressor is compressing 5 mol of gas (4 mol H2 and 1 mol CO2) for 1 mol of CH4 generated, whereas the second compressor only has to compress the lower volume of highly converted gas.
This study is a rare attempt to use Aspen Plus simulation environment in the field of bioprocess technology. Furthermore, to our knowledge, no simulation has been published so far which investigate the overall process efficiency for a biological methanogenesis process.
Based on experimental results published by Rittmann et al. [6] and Seifert et al. [27,33] an experimentally derived empiric kinetic model was used. The reaction unit was simulated using a model linking conversion rate to the limiting substrate concentration in order to obtain an estimation of the overall process efficiency using experimentally verified reaction rates.
In this simulation approach it was shown that Aspen Plus was useful to calculate vapor-liqui.equilibrium, and dissociation of investigated elements such as S, N and C source contained in the defined mineral media. This simulation concept is a valuable tool for improving elemental balancing of bioprocesses by estimating scrubbing or stripping rates for the substrates of interest. In later stages Aspen Plus simulation might be used as a tool for media design and adaptation to varying process conditions.
Furthermore, it was shown that Aspen Plus can be used as a valuable tool for estimation of bioprocess efficiency. The optimum pressure for the efficiency of the core reaction unit was found at 11 bar and differs from the optimum obtained for the integrated process, being at 2 bar. This large discrepancy can be explained by the fact, that 5 mol of reaction gas react to 1 mol of product gas, giving a 5 times higher volume for compression at compressor 1 compared to compressor 2. However, change in overall process efficiency with changing core unit pressure is small, varying from 66% to 70% if only the produced gas is taken into account and 73 to 76% if Qheat is added on the product side. Hence, optimum pressure in the core unit will rise in the integrated process, if a lower pressure at the purification unit is used. For more detailed efficiency analysis, a suitable compressor unit has to be modeled and loss of H2 in the purification step has to be considered. However, these results show the utility of Aspen Plus for similar bioprocess development as it account for the energetic aspect of an integrated process and not only individual steps.
State of the art “power to gas” technologies show similar efficiencies for formation of methane (Table 3). Since our study focuses only on the production of CH4 with H2 and CO2, the conversion of power to H2 has to be considered when comparing process efficiencies. Assuming maximum efficiency for the conversion of power to H2 (77%) and an efficiency of biological methanogenesis process (70%) obtained without considering obtained process heat gives an overall efficiency of about 54% for conversion of power to CH4, which is perfectly in line with data available for other technologies. Considering the heat obtained in the process a total efficiency of conversion could go as high as 65% which aligns with the best existing technologies for “Power to Gas” while performing at a much lower range of temperature and pressure than chemical processes. Therefore milder condition applied to this process will benefit the cost of investment.
The high efficiency of over 70% also demonstrates that biological methanogenesis is a promising alternative to the chemical transformation which offers as well a higher tolerance towards impurities.
Finally, Aspen Plus was proved to be an adaptable and useful tool for performing adaptive bioprocess efficiency simulation while implementing product formation kinetic models obtained experimentally at lab-scale. Although the simulated model cannot give insight into the biological process itself, it is highly useful for the layout and investigation of the integrated bioprocess. A detailed simulation could not only be used for process scale up, but also for optimization. Also the use for balancing similar bioprocesses could be of great interest.
The combination of Aspen Plus simulation with physiological investigation under bioreactor conditions is a new approach for bioprocess scale up and summing up the results, it was shown that Aspen Plus, although rarely used in this field, is a valuable tool in bioprocess technology.
The authors sincerely thank Dr. Simon Rittmann and Dr. Arne Seifert for their support and for providing the necessary experimental data.
All authors disclose to have no conflict of interests.
[1] | 2. Institute of Medicine. (2008) Retooling for an aging American: Building the healthcare workforce. Washington, DC: Academies Press. |
[2] |
3. Fisher E, Brownson C, O'Toole M, et al. (05) Ecological approaches to self-management: The case of diabetes. Am J Public Health 95: 1523-1535. doi: 10.2105/AJPH.2005.066084
![]() |
[3] | 4. Battersby M, Von Korff M, Schaefer J, et al. (2010) Twelve evidence-based principles for implementing self-management support in primary care. Joint Commis J Quality Patient Safety : 561-570. |
[4] | 5. Mercer S, Green L, Rosenthal A, et al. (2003) Possible lessons from the tobacco experience for obesity control. Am J Clin Nutr 77: 1073S-1082S. |
[5] |
6. Bodenheimer T, Lorig K, Holman H, et al. (2002) Patient self-management of chronic disease in primary care. J Am Med Assoc 288: 2469-247 doi: 10.1001/jama.288.19.2469
![]() |
[6] |
7. Goldzweig C, Orshansky G, Paige N, et al. (2013) Electronic patient portals: Evidence on health outcomes, satisfaction, efficiency, and attitudes: A systematic review. Ann Int Med 159: 7-687. doi: 10.7326/0003-4819-159-10-201311190-00006
![]() |
[7] |
8. Coughlin J, Pope J, Leedle B. (2006) Old age, new technology, and future innovations in disease management and home health care. Home Health Care Manag 18: 196-20 doi: 10.1177/1084822305281955
![]() |
[8] | 9. National Academy of Sciences. (2005) Facilitating interdisciplinary research. Washington, DC: National Academies Press. |
[9] | 10. Frey W. (2010) Baby boomers and the new demographics of America's seniors Generations 34: 28-37. |
[10] | 11. Robinson K, Reinhard S. (2009) Looking ahead in long-term care: The next 50 years. Nurs Clin North Am 44 (2): 253-262. |
[11] | 12. Stokols D. (1992) Establishing and maintaining healthy environments. Toward a social ecology of health promotion. Am Psychol 47: 6-22. |
[12] | 13. Hopkins D, Fielding J, Task Force on Community Preventive Services. (2001) The guide to community preventive services: Tobacco use prevention and control: Reviews, recommendations, and expert commentary. Am J Prev Med 20: S1-S88. |
[13] | 14. Patrick K, Intille S, Zabinski M. (2005) An ecological framework for cancer communication: Implications for research. J Med Int Res 7: e23. |
[14] |
15. Wagner E, Austin B, Von Korff M. (1996) Organizing care for patients with chronic illness. Milbank Quart 74: 511-544. doi: 10.2307/3350391
![]() |
[15] |
16. Coleman K, Austin B, Brach C, et al. (2009) Evidence on the Chronic Care Model in the new millennium. Health Affairs 28: 75-85. doi: 10.1377/hlthaff.28.1.75
![]() |
[16] | 17. Vincent G, Velkoff V. (2010) The next four decades: The older population in the United States, 2010 to 2050. Washington, DC: US Census Bureau. |
[17] |
18. Pruchno R. (2012) Not your mother's old age: Baby boomers at age 65. Gerontol 52: 149-152. doi: 10.1093/geront/gns038
![]() |
[18] | 19. Frey W. (2010) Baby boomers and the new demographics of American's seniors. Generations 34: 28-37. |
[19] | 20. Gassoumis Z, Wilber K, Baker L, et al. (2010) Who are the Latino baby boomers? Demographic and economic characteristics of a hidden population. J Aging Soc Policy 22: 53-68. |
[20] |
21. Lipschultz J, Hilt M, Reilly H. (7) Organizing the baby boomer construct: An exploration of marketing, social systems, and culture. Educ Gerontol 33: 759-773. doi: 10.1080/03601270701364511
![]() |
[21] |
22. Knickman JR, Snell EK. (2002) The 2030 problem: caring for aging baby boomers. Health Services Res 37: 849-884. doi: 10.1034/j.1600-0560.2002.56.x
![]() |
[22] | 23. Mochris G, Mathur A. (2007) Baby boomers and their parents: Surprising findings about their lifestyles, mindsets and well-being. Amarillo: Paramount Publishing. |
[23] |
24. Koch S. (2010) Healthy ageing supported by technology—a cross-disciplinary research challenge. Inform Health Soc Care 35: 81-91. doi: 10.3109/17538157.2010.528646
![]() |
[24] | 25. Barns P, Blooom B, Nihin R. (2008) Complementary and alternative use among adults and children: United States 2007. |
[25] |
26. Robinson K, Reinhard S. (2009) Looking ahead in long-term care: The next 50 years. Nurs Clin North Am 44: -262. doi: 10.1016/j.cnur.2009.02.004
![]() |
[26] |
27. Zwijsen SA, Niemeijer AR, Hertogh CM. (2011) Ethics of using assistive technology in the care for community-dwelling elderly people: an overview of the literature. Aging Ment Health 15: 419-427. doi: 10.1080/13607863.2010.543662
![]() |
[27] |
28. Steel DM, Gray MA. (2009) Baby boomers' use and perception of recommended assistive technology. International J Ther Rehab 16: 546-556. doi: 10.12968/ijtr.2009.16.10.44564
![]() |
[28] |
29. Mihailidis A, Cockburn A, Longley C, et al. (2008) The acceptability of home monitoring technology among community-dwelling older adults and baby boomers. Assist Technol 20: 1-12. doi: 10.1080/10400435.2008.10131927
![]() |
[29] | 30. Smith SP, Barefield AC. (2007) Patients meet technology. Health Care Manag 26: 354-362. |
[30] | 31. Zickuhr K. (2011) Generations and their gadgets. Washington: Pew Research Center's Internet & American Life Project, 1-20 |
[31] |
32. Andreassen H, Bujnowska-Fedak M, Chronaki C, et al. (2007) European citizens' use of e-health services: A study of seven countries. BMC Public Health 7: 53. doi: 10.1186/1471-2458-7-53
![]() |
[32] | 33. Tang PC, Lee HT. (2009) Your doctor's office or the internet? two paths to personal health records. New Engl J Med 360: 1276-1278. |
[33] | 34. Health on the Net Foundation. (1999) HON's fourth survey on the use of internet for medical and health purposes. . |
[34] |
35. Reisenwitz T, Iyer R. (2007) A comparison of younger and older baby boomers: investigating the viability of cohort segmentation. J Consum Marketing 24: 202-212. doi: 10.1108/07363760710755995
![]() |
[35] | 36. Sinden D, Wister AV. (2008) E-health promotion for aging baby boomers in north america. Gerontech J 7: 271-278. |
[36] | 37. Arora N, Hesse B, Rimer B, et al. (2007) Frustrated and confused: The American pubic rates it's cancer-related information-seeking experience J Gener Int Med 23: 223-228. |
[37] | 38. Tripp C, Straub L. (2008) Search for drug information: technology implications for rural consumers and pharmacies. Health Market Quart 18: 103-117. |
[38] |
39. Koch S. (2010) Healthy ageing supported by technology, a cross-disciplinary research challenge. Inform Health Soc Care 35: 81-91. doi: 10.3109/17538157.2010.528646
![]() |
[39] |
40. Chatterjee S, Price A. (2009) Health living with persuasive technologies: framework, issues and challenges. J Am Med Inform Assoc 16: 171-178. doi: 10.1197/jamia.M2859
![]() |
[40] | 41. Civan A, Skeels M, Stolyar A, et al. (2006) Personal health information management: Consumers' perspectives. AMIA Annual Symposium Proceedings: 156-160. |
[41] | 42. Agarwal R, Khuntia J. (2009) Personal health information and the design of consumer health information technology: Background report. Rockville: Agency for Healthcare Research and Quality. |
[42] | 43. Wilson C, Peterson A. (2010) Managing personal health information: An action agenda Rockville, MD: Insight Policy Research AHRQ Publication No. 10-0048- |
[43] |
44. Noh H-I, Lee JM, Yun YH, et al. (2009) Cervical cancer patient information-seeking behaviors, information needs, and information sources in South Korea. Supp Care Cancer 17: 1277-1283. doi: 10.1007/s00520-009-0581-y
![]() |
[44] |
45. Caiata-Zufferey M, Abraham A, Sommerhalder K, et al. (2010) Online health information seeking in the context of the medical consultation in Switzerland. Qualit Health Res 20: 1050-1061. doi: 10.1177/1049732310368404
![]() |
[45] |
46. Manafo E, Wong S. (2012) Exploring older adults' health information seeking behaviors. J Nutr Educ Behav 44: 85-89. doi: 10.1016/j.jneb.2011.05.018
![]() |
[46] |
47. Kim K, Kwon N. (2010) Profile of e-patients: analysis of their cancer information-seeking from a national survey. J Health Commun 15: 712-733. doi: 10.1080/10810730.2010.514031
![]() |
[47] | 48. Higgins O, Sixsmith J, Barry M, et al. (2011) A literature review on healht information seeking behaviour on teh web: A health consumer and health professional perspective. Stockholm: European Center for Disease Prevention and Control. |
[48] |
49. Eheman CR, Berkowitz Z, Lee J, et al. (2009) Information-seeking styles among cancer patients before and after treatment by demographics and use of information sources. J Health Commun 14: -502. doi: 10.1080/10810730903032945
![]() |
[49] | 50. Kelly KM, Sturm AC, Kemp K, et al. (2009) How can we reach them? Information seeking and preferences for a cancer family history campaign in underserved communities. J Health Commun 14: 573-589. |
[50] |
51. Kelly B, Hornik R, Romantan A, et al. (2010) Cancer information scanning and seeking in the general population. J Health Commun 15: 734-753. doi: 10.1080/10810730.2010.514029
![]() |
[51] |
52. Galarce EM, Ramanadhan S, Weeks J, et al. (2011) Class, race, ethnicity and information needs in post-treatment cancer patients. Patient Educ Counsel 85: 432-439. doi: 10.1016/j.pec.2011.01.030
![]() |
[52] |
53. Sung VW, Raker CA, Myers DL, et al. (2010) Treatment decision-making and information-seeking preferences in women with pelvic floor disorders. Int Urogynecol J 21: 1071-1078. doi: 10.1007/s00192-010-1155-8
![]() |
[53] | 54. Fox S. (2007) E-patients with disability or chronic disease. Washington, DC: Pew Internet & American Life Project. |
[54] | 55. Fox S, Jones S. (2009) The social lifew of health information. Washington, DC: Pew Internet & American Life Project. |
[55] | 56. Radina ME, Ginter AC, Brandt J, et al. (2011) Breast cancer patients' use of health information in decision making and coping. Cancer Nurs 34: E1-12. |
[56] |
57. Smith SK, Dixon A, Trevena L, et al. (2009) Exploring patient involvement in healthcare decision making across different education and functional health literacy groups. Soc Sci Med 69: 1805-1812. doi: 10.1016/j.socscimed.2009.09.056
![]() |
[57] |
58. Weaver J 3rd, Mays D, Weaver S, et al. (2010) Health information-seeking behaviors, health indicators, and health risks. American J Public Health 100: 1520-1525. doi: 10.2105/AJPH.2009.180521
![]() |
[58] | 59. Agarwal R, Angst C. (2006) Technology-enabled transformations in health care: Early findings on personal health records and invidual use In: Galletta D, Zhang P, editors. Human-computer interaction and management information systems: Application. Armonk, NY: M. E. Sharp, 357-378. |
[59] |
60. Tawara S, Yonemochi Y, Kosaka T, et al. (2013) Use of patients' mobile phones to store and share personal health information: Results of a questionnaire survey. Int Med 52: 751-756. doi: 10.2169/internalmedicine.52.9030
![]() |
[60] |
61. Siek K, Khan D, Ross S, et al. (2011) Designing a personal health application for older adults to manage medications: a comprehensive case study. J Med Syst 35: 1099-1121. doi: 10.1007/s10916-011-9719-9
![]() |
[61] |
62. Moen A, Brennan PF. (2005) Health@Home: the work of health information management in the household (HIMH): implications for consumer health informatics (CHI) innovations. J Am Me Inform Assoc 12: 648-656. doi: 10.1197/jamia.M1758
![]() |
[62] | 63. Marchionini G, Rimer B, Wildemuth B. (2007) Evidence base for personal health record usability: final report to the National Cancer Institute. Chapel Hill: University of North Carolina Chapel Hill. |
[63] |
64. Maiorana A, Steward W, Koester K, et al. (2012) Trust, confidentiality, and the acceptability of sharing HIV-related patient data: lessons learned from a mixed methods study about health information exchanges. Implem Sci 7: 34. doi: 10.1186/1748-5908-7-34
![]() |
[64] | 65. Avtgis T, Polack E, Staggers S, et al. (2011) Health provider-recipient interactions: Is “online interaction” the next best thing to “being there?” In: Wright K, Webb L, editors. Computer-medicated communication in interpersonal relationships London: Peter Lang. |
[65] | 66. Grande N, Mitra N, Shah A, et al. (2013) Public preferences about secondary uses of electronic health information. J Am Med Assoc Int Med 173: 1798-1806. |
[66] |
67. Hunter I, Whiddett R, Norris A, et al. (2009) New Zealanders' attitudes towards access to their electronic health records: Preliminary results from a national study using vignettes. Health Inform J 15: 212-228. doi: 10.1177/1460458209337435
![]() |
[67] |
68. King T, Brankovic L, Gillard P. (2012) Perspectives of Australian adults about protecting the privacy of their health information in statistical databases. Int J Med Inform 81: 279-289. doi: 10.1016/j.ijmedinf.2012.01.005
![]() |
[68] | 69. Page S, Mitchell I. (2006) Patients' opinions on privacy, consent and the disclosure of health information for medical research. Chron Dis Canada 27: 60-67. |
[69] |
70. Caine K, Hanania R. (2013) Patients want granular privacy control over health information in electronic medical records. J Am Med Inform Assoc AMIA 20: 7-15. doi: 10.1136/amiajnl-2012-001023
![]() |
[70] | 71. Reason J. (1990) Human Error. New York: Cambridge University Press. |
[71] |
72. Street R Jr. (2007) Aiding medical decision making: A communication perspective. Med Dec Making 27: 550-553. doi: 10.1177/0272989X07307581
![]() |
[72] | 73. Gochman DS. (1997) Handbook of health behavior research. New York: Plenum Press. |
[73] | 74. Glanz K, Rimer B, Viswanath K. (2008) Health behavior and health education: Theory, research, and practice. San Francisco: Jossey-Bass. |
[74] | 75. Agency for Healthcare Research and Quality. (2000) Outcomes research fact sheet. Agency for Healthcare Research and Quality, 0-11. |
[75] | 76. Attias-Confut C, Wolff F. (2000) The redistributive effects of generational transfers. In: Arbur S, Attias-Confut C, editors. The myth of generational conflict: The family and state in ageing societies. New York: Routledge, 22-46. |
[76] | 77. Lin I. (2008) Consequences of parental divorce for adult children's support of their frail parents J Marr Family 70: 113-128. |
[77] | 79. Centers for Disease Control and Prevention. (2013) The state of aging and health in America 2013. Atlanta: U. S. Department of Health and Human Services. |
[78] | 80. Ahn S, Smith M, Dicerhson J, et al. (2012) Health and health care utilization among obese and diabetic baby boomers and older adults Am J Health Prom 27: 123-132. |
[79] | 81. Martin L, Freedman V, Schoeni R, et al. (2009) Health and functioning of the Baby Boom approaching 60. J Gerontol Soc Sci 63: 369-377. |
[80] | 82. King D, Matheson E, Chirina S, et al. (2013) The status of Baby Boomers' health in the United States: The healthiest generation? J Am Med Assoc Int Med 173: 385-386. |
[81] |
83. Wagner E, Austin B, Von Korff M. (1996) Organizing care for patients with chronic illness. Milbank Quart 74: 511-544. doi: 10.2307/3350391
![]() |
[82] | 84. Civan A, Skeels M, Stolyar A, et al. (2006) Personal health information management: Consumers' perspectives. AMIA Annual Symposium Proceedings, 156-160. |
[83] |
85. Miller N, Berra K, Long J. (2010) Hypertension 2008--awareness, understanding, and treatment of previously diagnosed hypertension in baby boomers and seniors: A survey conducted by Harris interactive on behalf of the Preventive Cardiovascular Nurses Association. J Clin Hypert 12: 328-334. doi: 10.1111/j.1751-7176.2010.00267.x
![]() |
[84] | 86. Koh H, Baur C, Brach C, et al. (2013) Toward a systems approach to health literacy research. J Health Commun 18: 1-5. |
[85] |
87. Rudd R. (2010) Improving Americans' health literacy. New Engl J Med 363: 2283-22 doi: 10.1056/NEJMp1008755
![]() |
[86] |
88. Bell RA, Hu X, Orrange SE, et al. (2011) Lingering questions and doubts: online information-seeking of support forum members following their medical visits. Patient Educ Counsel 85: 525-528. doi: 10.1016/j.pec.2011.01.015
![]() |
[87] | 89. Tustin N. (2010) The role of patient satisfaction in online health information seeking. J Health Commun 15: 3-17. |
[88] | 90. Court D, Farrell D, Forsyth J. (2007) Serving aging baby boomers. McKinsey Quart 104: 102-113. |
[89] | 91. Ganong L, Coleman M. (1999) Changing families, changing responsibilities: Family obligations following divorce and remarriage. Mahwah, NJ: Lawrence Erlbaum. |
[90] |
92. Fingerman K, Pillemer K, Silverstein M, et al. (2012) The Baby Boomers' intergenerational relationships. Gerontologist 52: 199-209. doi: 10.1093/geront/gnr139
![]() |
[91] | 93. Nussbaum J (1994) Friendship in older adulthood. In: Hummert M, Weimann J, Nussbaum J, editors. Interpersonal communication in older adulthood. Thousand Oaks, CA: Sage, 209-225. |
[92] | 94. Brennan P, Safran C. (2005) Empowered consumers. In: Lewis D, Eysenbach G, Kukafka R, et al. , editors. Consumer health informatics: Informing consumers and improving health care New York: Springer, 8-21. |
[93] |
95. Longo DR, Schubert SL, Wright BA, et al. (2010) Health information seeking, receipt, and use in diabetes self-management. Ann Family Med 8: 334-340. doi: 10.1370/afm.1115
![]() |
[94] |
96. Kreuter M, Alcaraz K, Pfeiffer D, et al. (2008) Using dissemination research to identify optimal community settings for tailored breast cancer information kiosks. J Public Health Manag Pract 14: 160-169. doi: 10.1097/01.PHH.0000311895.57831.02
![]() |
[95] | 97. Fogg B. (2003) Persuasive technology, using computers to change what we think and do. San Francisco, CA: Morgan Kaufmann. |
1. | Bernhard Lecker, Lukas Illi, Andreas Lemmer, Hans Oechsner, Biological hydrogen methanation – A review, 2017, 245, 09608524, 1220, 10.1016/j.biortech.2017.08.176 | |
2. | Yan Rafrafi, Léa Laguillaumie, Claire Dumas, Biological Methanation of H2 and CO2 with Mixed Cultures: Current Advances, Hurdles and Challenges, 2020, 1877-2641, 10.1007/s12649-020-01283-z | |
3. | Sébastien Bernacchi, Simon Rittmann, Arne H. Seifert, Alexander Krajete, Christoph Herwig, Experimental methods for screening parameters influencing the growth to product yield (Y(x/CH4)) of a biological methane production (BMP) process performed with Methanothermobacter marburgensis, 2014, 1, 2375-1495, 72, 10.3934/bioeng.2014.2.72 | |
4. | Birgit Hoff, Jens Plassmeier, Matthew Blankschien, Anne‐Catrin Letzel, Lauralynn Kourtz, Hartwig Schröder, Walter Koch, Oskar Zelder, Unlocking Nature's Biosynthetic Power—Metabolic Engineering for the Fermentative Production of Chemicals, 2021, 133, 0044-8249, 2288, 10.1002/ange.202004248 | |
5. | Annalisa Abdel Azim, Christian Pruckner, Philipp Kolar, Ruth-Sophie Taubner, Debora Fino, Guido Saracco, Filipa L. Sousa, Simon K.-M.R. Rittmann, The physiology of trace elements in biological methane production, 2017, 241, 09608524, 775, 10.1016/j.biortech.2017.05.211 | |
6. | Grazia Leonzio, Process analysis of biological Sabatier reaction for bio-methane production, 2016, 290, 13858947, 490, 10.1016/j.cej.2016.01.068 | |
7. | Lisa-Maria Mauerhofer, Patricia Pappenreiter, Christian Paulik, Arne H. Seifert, Sébastien Bernacchi, Simon K.-M. R. Rittmann, Methods for quantification of growth and productivity in anaerobic microbiology and biotechnology, 2019, 64, 0015-5632, 321, 10.1007/s12223-018-0658-4 | |
8. | Johanny Pestalozzi, Claudia Bieling, Dirk Scheer, Cordula Kropp, Integrating power-to-gas in the biogas value chain: analysis of stakeholder perception and risk governance requirements, 2019, 9, 2192-0567, 10.1186/s13705-019-0220-5 | |
9. | Simon K.-M.R. Rittmann, Arne H. Seifert, Sébastien Bernacchi, Kinetics, multivariate statistical modelling, and physiology of CO2-based biological methane production, 2018, 216, 03062619, 751, 10.1016/j.apenergy.2018.01.075 | |
10. | Simon K.-M. R. Rittmann, Arne H. Seifert, Alexander Krajete, Biomethanisierung — ein Prozess zur Ermöglichung der Energiewende?, 2014, 20, 0947-0867, 816, 10.1007/s12268-014-0521-3 | |
11. | Birgit Hoff, Jens Plassmeier, Matthew Blankschien, Anne‐Catrin Letzel, Lauralynn Kourtz, Hartwig Schröder, Walter Koch, Oskar Zelder, Unlocking Nature's Biosynthetic Power—Metabolic Engineering for the Fermentative Production of Chemicals, 2021, 60, 1433-7851, 2258, 10.1002/anie.202004248 | |
12. | Sébastien Bernacchi, Alexander Krajete, Christoph Herwig, Experimental workflow for developing a feed forward strategy to control biomass growth and exploit maximum specific methane productivity of Methanothermobacter marburgensis in a biological methane production process (BMPP), 2016, 2, 2471-1888, 262, 10.3934/microbiol.2016.3.262 | |
13. | Joanna Kazimierowicz, Marcin Dębowski, Marcin Zieliński, Effectiveness of Hydrogen Production by Bacteroides vulgatus in Psychrophilic Fermentation of Cattle Slurry, 2022, 4, 2571-8797, 806, 10.3390/cleantechnol4030049 | |
14. | Eduardo Sánchez Nocete, Javier Pérez Rodríguez, A Simple Methodology for Estimating the Potential Biomethane Production in a Region: Application in a Case Study, 2022, 14, 2071-1050, 15978, 10.3390/su142315978 | |
15. | Eike Janesch, Joana Pereira, Peter Neubauer, Stefan Junne, Phase Separation in Anaerobic Digestion: A Potential for Easier Process Combination?, 2021, 3, 2673-2718, 10.3389/fceng.2021.711971 | |
16. | Pauls P. Argalis, Kristine Vegere, Perspective Biomethane Potential and Its Utilization in the Transport Sector in the Current Situation of Latvia, 2021, 13, 2071-1050, 7827, 10.3390/su13147827 | |
17. | Monika Vítězová, Vladimír Onderka, Iva Urbanová, Anna Molíková, Nikola Hanišáková, Iva Buriánková, Tomáš Vítěz, David Novák, Jan Lochman, Markéta Machálková, Jakub Javůrek, In situ field experiment shows the potential of methanogenic archaea for biomethane production from underground gas storage in natural rock environment, 2023, 23521864, 103253, 10.1016/j.eti.2023.103253 | |
18. | 2024, 9783527352753, 1, 10.1002/9783527843954.ch1 | |
19. | Zohreh Safari, Rouhollah Fatehi, Reza Azin, Developing a numerical model for microbial methanation in a depleted hydrocarbon reservoir, 2024, 09601481, 120426, 10.1016/j.renene.2024.120426 |
Unit | Type | Temperature | Pressure |
Reactor | equilibrium | 65℃ | 1 bar-11 bar |
Compressor 1 | isentropic | 65℃ | 1 bar-11 bar |
Compressor 2 | isentropic | 65℃ | 21 bar |
Case | Pressure at reactor [bar] | Pressure at separation unit [bar] | Efftot1 (Eq. 4) [%] | Efftot2 (Eq. 5) [%] |
A) | 1 | 21 | 67.91 | 73.18 |
B) | 2 | 21 | 70.16 | 75.74 |
C) | 6 | 21 | 68.09 | 73.93 |
D) | 11 | 21 | 66.62 | 74.21 |
Path | Efficiency | Conditions |
Electricity to gas | ||
electricity →H2 | 54-72% | at compression to 200 bar (working pressure of most gas storage plants) |
electricity →CH4 (SNG) | 49-64% | |
electricity →H2 | 57-73% | at compression to 80 bar (feed long distance/transmission pipeline) |
electricity →CH4 (SNG) | 50-64% | |
electricity →H2 | 64-77% | without compression |
electricity →CH4 (SNG) | 51-65% | |
electricity to gas to electricity | ||
electricity →H2→ electricity | 34-44% | at electrification with 60% and compression to 80 bar |
electricity →CH4 (SNG) → electricity | 30-38% | |
electricity to gas to electricity (cogeneration, combined heat and power, CHP) | ||
electricity →H2→ electricity (CHP) | 48 - 62% | at 40% conversion efficiency for electricity, 45% efficiency for heat and compression to 80 bar |
electricity →CH4 (SNG) → electricity (CHP) | 43 - 54% |
Unit | Type | Temperature | Pressure |
Reactor | equilibrium | 65℃ | 1 bar-11 bar |
Compressor 1 | isentropic | 65℃ | 1 bar-11 bar |
Compressor 2 | isentropic | 65℃ | 21 bar |
Case | Pressure at reactor [bar] | Pressure at separation unit [bar] | Efftot1 (Eq. 4) [%] | Efftot2 (Eq. 5) [%] |
A) | 1 | 21 | 67.91 | 73.18 |
B) | 2 | 21 | 70.16 | 75.74 |
C) | 6 | 21 | 68.09 | 73.93 |
D) | 11 | 21 | 66.62 | 74.21 |
Path | Efficiency | Conditions |
Electricity to gas | ||
electricity →H2 | 54-72% | at compression to 200 bar (working pressure of most gas storage plants) |
electricity →CH4 (SNG) | 49-64% | |
electricity →H2 | 57-73% | at compression to 80 bar (feed long distance/transmission pipeline) |
electricity →CH4 (SNG) | 50-64% | |
electricity →H2 | 64-77% | without compression |
electricity →CH4 (SNG) | 51-65% | |
electricity to gas to electricity | ||
electricity →H2→ electricity | 34-44% | at electrification with 60% and compression to 80 bar |
electricity →CH4 (SNG) → electricity | 30-38% | |
electricity to gas to electricity (cogeneration, combined heat and power, CHP) | ||
electricity →H2→ electricity (CHP) | 48 - 62% | at 40% conversion efficiency for electricity, 45% efficiency for heat and compression to 80 bar |
electricity →CH4 (SNG) → electricity (CHP) | 43 - 54% |