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Review

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications

  • Exopolysaccharides (EPSs) are biological polymers secreted by microorganisms including Lactic acid bacteria (LAB) to cope with harsh environmental conditions. EPSs are one of the main components involved in the formation of extracellular biofilm matrix to protect microorganisms from adverse factors such as temperature, pH, antibiotics, host immune defenses, etc.. In this review, we discuss EPS biosynthesis; the role of EPSs in LAB stress tolerance; the impact of environmental stresses on EPS production and on the expression of genes involved in EPS synthesis. The evaluation results indicated that environmental stresses can alter EPS biosynthesis in LAB. For further studies, environmental stresses may be used to generate a new EPS type with high biological activity for industrial applications.

    Citation: Phu-Tho Nguyen, Tho-Thi Nguyen, Duc-Cuong Bui, Phuoc-Toan Hong, Quoc-Khanh Hoang, Huu-Thanh Nguyen. Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications[J]. AIMS Microbiology, 2020, 6(4): 451-469. doi: 10.3934/microbiol.2020027

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  • Exopolysaccharides (EPSs) are biological polymers secreted by microorganisms including Lactic acid bacteria (LAB) to cope with harsh environmental conditions. EPSs are one of the main components involved in the formation of extracellular biofilm matrix to protect microorganisms from adverse factors such as temperature, pH, antibiotics, host immune defenses, etc.. In this review, we discuss EPS biosynthesis; the role of EPSs in LAB stress tolerance; the impact of environmental stresses on EPS production and on the expression of genes involved in EPS synthesis. The evaluation results indicated that environmental stresses can alter EPS biosynthesis in LAB. For further studies, environmental stresses may be used to generate a new EPS type with high biological activity for industrial applications.


    In this paper, we are concerned with the existence of positive solutions for the following Kirchhoff equation in RN:

    (a+bRN|u|2dx)Δu+λu=μ|u|q2u+|u|p2u,(K)

    having prescribed mass

    RN|u|2dx=c2, (1.1)

    where c>0 is prescribed, N4, μR, a>0 and b>0 are positive parameters, 2<q<p2, 2 is the Sobolev critical exponent, 2=2NN2 if N3, 2=+ if N=1,2.

    Problem (K) is analogous to the stationary case of the following equation:

    utt(a+bΩ|u|2dx)Δu=f(x,u),(x,t)Ω×R+,

    where ΩRN is a smooth domain, u stands for the displacement, fC(Ω×R,R) is the external force, a is the initial tension and b is related to the intrinsic properties of the string. Such a hyperbolic equation is a general version of the following equations

    ρ2ut2(P0h+E2LL0|ux|2dx)2ux2=0,

    proposed by Kirchhoff [1] as an extension of the classical D'Alembert's wave equation for transversal oscillations of a stretched string, particularly, taking into account the subsequent change in string length during the oscillations. In addition, nonlocal problems similar to (K) also arise in biological systems that can be applied to describe the growth and movement of a specific species[2]. In this situation, u may describe a process that depends on the average of itself, for example, the population density.

    After the pioneering work of [3], problem (K) with fixed λ, or even with an additional external and fixed potential V(x) has been extensively studied in the last ten years, see e.g., [2,4,5,6,7] and the references therein. Compared with Schrödinger type problem, the main difficulty for Kirchhoff type problem is how to obtain the compactness of the Palais-Smale sequence. Tang-Cheng [5] proposed a new approach to recover the compactness for the Palais-Smale sequences. Tang-Chen [6] proposed a new approach to recover the compactness for the minimizing sequences.

    In this situation, it is well known that solutions of (K) are the critical points of the associated energy functional

    Φλ(u):=12RN(a|u|2+λ|u|2)dx+b4(RN|u|2dx)2μqRN|u|qdx1pRN|u|pdx.

    In the present paper, motivated by the fact that physicists are more interested in solutions satisfying the prescribed mass (1.1) which are called normalized solutions, we search for solutions of (K) having a prescribed L2-norm. This type of solutions can be obtained as a constrained critical point of the following functional

    I(u)=a2RN|u|2dx+b4(RN|u|2dx)2μqRN|u|qdx1pRN|u|pdx, (1.2)

    on the L2-sphere

    S(c)={uH1(RN):uL2(RN)=c},

    where the Hilbert space H1(RN) is defined as

    H1(RN):={uL2(RN):RN|u|2dx<},

    with the inner product and norm given by

    (u,v)H1(RN)=RN(uv+uv)dx,uH1(RN)=(u2L2(RN)+u2L2(RN))12.

    When a=1,b=μ=0, problem (K) is reduced to the classical Schrödinger equation with pure power nonlinearity:

    Δu+λu=|u|p2u,xRN, (1.3)

    where N1, p(2,2). It is well known that the L2-critical exponent

    ˉp=2+4N,

    plays an important role in dealing with the Schrödinger equation. For the pure power nonlinearity |u|p2u satisfying L2-subcritical growth, i.e., 2<p<ˉp, then the energy functional associated to (1.3) is bounded from below. Cazenave-Lions [8] and Shibata [9] proved the existence of normalized solution via minimizing methods. We also refer the reader to the references [10,11]. While for L2-supercritical and Sobolev subcritical case ˉp<p<2, the energy functional associated to (1.3) cannot be minimized on the L2-sphere S(c). Jeanjean [12] proved for the first time the existence of a normalized solution in this case. When a=1,b=0 and μ0, problem (K) is reduced to the Schrödinger equation with combined power nonlinearities:

    Δu+λu=μ|u|q2u+|u|p2u,xRN, (1.4)

    where N1, μR, the nonlinearity satisfies the mixed growth, i.e., 2<qˉpp2. Recently, a very complete analysis of the various cases that may occur for (1.4), depending on the values of p and q, has been studied exhaustively in [13,14]. The paper [13] deals with the Sobolev subcritical case and [14] with Sobolev critical case.

    It is well known that two exponents play a special role for existence and properties of the solutions for the Kirchhoff equation, in addition to the energy (Sobolev) critical exponent

    2=2NN2,

    we have the mass (L2-) critical one

    2+8N.

    The mass and energy critical exponents define the thresholds for the existence of global minima and H1-solution, respectively. It follows from

    2+8N<2NN2

    that N3. For this reason possibly, there are many results on normalized solution of Kirchhoff problem in low dimensions N3, we refer the readers to [15,16,17,18,19] and references therein. Howover, to the best of our knowledge, the Kirchhoff type problems with prescribed L2-norm in high dimensions N4 remains unsolved in the existing literature. This motivates us to study this kind of problem in the present paper for the case of N=4,6,8. When N4, since 24, it is difficult to study the geometric structure of the energy functional. Also, it is not easy to prove the boundedness and convergence of the Palais-Smale sequence.

    Particularly, in dimension N=4, there is a special phenomenon for Kirchhoff problems that will never happen for the Schrödinger equation, i.e., the mass critical exponent 2+8N is the same as the energy critical exponent 2=2NN2. We call this special phenomenon the new doubly critical case, which is first considered to happen for the Kirchhoff equation in this paper. For other types of double critical exponents, we refer the readers to [20,21] and references therein. Moreover, it is worth mentioning that when N4, if we consider the mass (L2-) supercritical case, the problem will no longer have a variational structure owing to the fact that the mass critical exponent is larger than the energy critical exponent. Thus, there will be no mass supercritical and mixed cases.

    Motivated by the works mentioned above, our contribution in this paper is to find appropriate transformation to get the following equivalent system of (K) in different dimension spaces with respect to (v,β)RN×R+:

    {Δv+λv=μ|v|q2v+|v|p2v,βabβN22RN|v|2dx=0,RN|v|2dx=˜βN2Nc2,(PN)

    where

    ˜βN={a1d,N=4,1±14ad2d,N=6,23dcos(θ+2jπ3)(j=0,2),N=8, (1.5)

    θ=13arccos(323da)(π6,π3), and

    d=bv2L2(RN). (1.6)

    We take two steps to get the existence of solutions for system (PN). First, we solve the equation

    {Δv+λv=μ|v|q2v+|v|p2v,RN|v|2dx=˜βN2Nc2, (1.7)

    for the special given ˜βN. Then, when v is known, coincidentally, ˜βN can solve the algebra equation

    βabβN22RN|v|2dx=0. (1.8)

    Therefore, Some known difficulties can be overcome by establishing equivalent results, which is the key ingredient to prove the nonexistence, existence and multiplicity of normalized solutions for the Kirchhoff equation (K).

    Theorem 1.1. Let N=4,6,8. Then equation (K) has at least one positive, radially symmetric normalized solution uS(c) if and only if the system (PN) has at least one solution (v,˜βN)S(˜βN4Nc)×R+ such that u is positive and radially symmetric, where ˜βN is defined by (1.5).

    To state our another main results, we introduce some frequently used constants. For p(2,2), we use the notation

    γp:=N(p2)2p,

    and we denote by CN,p the best constant in the Gagliardo-Nirenberg inequality[22]

    uLp(RN)CN,puγpL2(RN)u1γpL2(RN),uH1(RN).

    In the special case p=ˉp, we denote by ˉCN(β)=(ˉpβ2CˉpN,ˉp)N4 the critical mass. For 2<qˉp<p<2 and μ>0, we denote:

    μ(β)=(p(2γqq)2CpN,p(γppγqq))2γqqγpp2(q(γpp2)2CqN,q(γppγqq))((βN4c)(1γq)q)((βN4c)(1γp)p)2γqqγpp2,μ(β)=ˉpβ2CˉpN,ˉpc4N.

    For 2<qˉp<p<2 and μ<0, we denote:

    μ(β)=(1γpCqN,q(γpγq))(1γpCpN,p)2γqqγpp2((βN4c)(1γq)q)((βN4c)(1γp)p)2γqqγpp2.

    For 2<q<ˉp<p=2 and μ>0, we denote:

    ˆμ(β)=(2S22(2γqq)2(2γqq))2γqq22(q(22)2CqN,q(2γqq)(βN4c)(1γq)q),ˇμ(β)=22NγqCqN,q(2γqq)(βN4c)(1γq)q(γqqSN22γqq)2γqq2.

    For q=ˉp<p=2 and μ>0, we denote:

    ˉμ(β)=ˉp2CˉpN,ˉpc4N(βN4c)(1γq)q.

    For ˉp<q<p=2 and μ>0, we denote:

    μ(β)=+ if N=4, and μ(β)=SN4(1γq)qγq(βN4c)(1γq)q if N5,

    where the optimal Sobolev embedding constant S[23] is given by

    S=infuD1,2(RN){0}u2L2(RN)u2L2(RN),

    where D1,2(RN) denotes the completion of C0(RN) with respect to the semi-norm uD1,2(RN):=uL2(RN).

    Applying Theorem 1.1, we can prove the following results. Firstly, in the Sobolev subcritical case 2<q<p=ˉp or 2<qˉp<p<2, we have:

    Theorem 1.2. Suppose that a,b>0 and 2<q<p<2 hold. Let ˜βN and d be defined by (1.5) and (1.6), respectively. If one of the following conditions holds:

    (1)2<q<p=ˉp, 0<c<ˉCN(˜βN) and μ>0;

    (2)2<qˉp<p<2, c>0 and 0<μ<min{μ(˜βN),μ(~βN)};

    (3)2<qˉp<p<2, c>0 and μ(˜βN)<μ<0;

    then we have the following conclusions:

    (i) for N=4, equation (K) has a positive, radially symmetric solution uS(c) if d(0,1), and has no nontrivial solution if d[1,+);

    (ii) for N=6,8, equation (K) has two positive, radially symmetric solutions u1, u2S(c) if d(0,dN), has one positive, radially symmetric solution uS(c) if d=dN, and has no nontrivial solution if d(dN,+), where dN=14a when N=6 and dN=427a2 when N=8.

    Next, in the Sobolev critical case 2<q<p=2, we have the following result:

    Theorem 1.3. Suppose that a,b,c>0 and 2<q<p=2 hold. Let ˜βN and d be defined by (1.5) and (1.6), respectively. If one of the following conditions holds:

    (1)2<q<ˉp<p=2 and 0<μ<min{ˇμ(˜βN),ˆμ(˜βN)};

    (2)ˉp=q<p=2 and 0<μ<ˉμ(˜βN);

    (3)ˉp<q<p=2 and 0<μ<μ(˜βN);

    then we have the following conclusions:

    (i) for N=4, equation (K) has a positive, radially symmetric solution uS(c) if d(0,1), and has no nontrivial solution if d[1,+);

    (ii) for N=6,8, equation (K) has two positive, radially symmetric solutions u1, u2S(c) if d(0,dN), has one positive, radially symmetric solution uS(c) if d=dN, and has no nontrivial solution if d(dN,+), where dN=14a when N=6 and dN=427a2 when N=8.

    Remark 1.1. Theorem 1.3 covers the doubly critical case that the mass critical exponent is the same as the energy critical exponent i.e., in dimension N=4,

    p=2=2+8N.

    Remark 1.2. As far as we know, Theorems 1.2 and 1.3 seem to be the first results on the existence and multiplicity of normalized solutions for equation (K) in high dimensions N=4,6,8. We also remark that it is interesting to know whether (K) has a normalized solution for aN42b small in more general dimensions N4. However, we can not solve this question now. So far, we just know that (K) has at least a positive normalized solution for aN42b small, and has no nontrivial solution for aN42b large in the special dimensions N=4,6,8. Moreover, it is reasonable to regard a and b as parameters in (K) since, as already noted before, a is the initial tension and b is related to the intrinsic properties of the string.

    Finally, in the doubly Sobolev critical case q=p=2, based on the Pohožaev identity, we have the following nonexistence result, which is not related to the equivalent results but interesting in itself.

    Theorem 1.4. Suppose that p=q=2 and N=4 holds, then equation (K) has no positive solutions uS(c) for any μ>0.

    This paper is organized as follows: In Section 2, we present some preliminaries. In Section 3, we show some results of (PN). In Section 4, we give the proofs of Theorems 1.1–1.4.

    In this section, we introduce some preliminaries that will be used to prove Theorems 1.2–1.4.

    Lemma 2.1. Let μ>0, N4 and uS(c) be a solution of (K). If u0 and u0, then λ>0.

    Proof. Arguing indirectly, suppose that λ0. It follows from u0 that

    (a+bRN|u|2dx)Δu=λu+μ|u|q2u+|u|p2u0,

    Thus Δu0. Moreover, from standard regularity results we have that uC2(RN). By the Liouville's result [24,Lemma A.2], we obtain that u0. This contradicts with u0. Thus λ>0.

    Lemma 2.2. Let a,b>0, p,q(2,2] and μ,λR. If uH1(RN) is a weak solution of the Kirchhoff equation (K), then the following Pohožaev identity holds:

    P(u)=au2L2(RN)+bu4L2(RN)μγquqLq(RN)γpupLp(RN)=0. (2.1)

    Proof. Let M=u2L2(RN), then uH1(RN) is a weak solution of the following problem

    Δu=1a+bM(μ|u|q2u+|u|p2uλu).

    Then the elliptic regularity theory implies that uC2(RN). Combining

    au2L2(RN)+bu4L2(RN)=μuqLq(RN)+upLp(RN)λu2L2(RN)

    with

    au2L2(RN)+bu4L2(RN)=2NN2[μquqLq(RN)+1pupLp(RN)λ2u2L2(RN)],

    we conclude that (2.1) holds.

    Lemma 2.3. For m,nR and (n2)2+(m3)3<0, then the following cubic

    x3+mx+n=0, (2.2)

    has three distinct real roots, which can be written in the form

    xj=23rcos(θ+2jπ3)(j=0,1,2), (2.3)

    where r=(m3)3 and θ=13arccos(n2r).

    Proof. This lemma is a special case of Cardano's formulas, and for the convenience of the reader, we give a simple proof. Set

    m=3yz and n=y3z3, (2.4)

    it follows from the Binomial Theorem that x=y+z always satisfies (2.2). From (2.4), we can obtain that y3 and z3 are the two roots of the quadratic

    X2+nXm327=0.

    Furthermore, by (n2)2+(m3)3<0, without loss of generality, we may assume that

    y3=n2+i(n2)2(m3)3=r(cos(3θ)+isin(3θ)),

    and

    z3=n2i(n2)2(m3)3=r(cos(3θ)isin(3θ)),

    where r=(m3)3 and θ=13arccos(n2r)(0,π3). It follows that

    y=3r(cos(θ+2jπ3)+isin(θ+2jπ3))(j=0,1,2),

    and

    z=3r(cos(θ+2jπ3)isin(θ+2jπ3))(j=0,1,2).

    Note that x=y+z, we then conclude that (2.2) has three distinct real roots, which can be expressed as in (2.3).

    We devote to solve system (PN) in this section. We will prove the following results which will be used to conclude Theorems 1.2 and 1.3.

    Firstly, in the Sobolev subcritical case 2<q<p=ˉp or 2<qˉp<p<2, we have:

    Proposition 3.1. Suppose that a>0 and 2<q<p<2 hold. Let ˜βN and d be defined by (1.5) and (1.6), respectively. If one of the following conditions holds:

    (1)2<q<p=ˉp, 0<c<ˉCN(˜βN) and μ>0;

    (2)2<qˉp<p<2, c>0 and 0<μ<min{μ(˜βN),μ(˜βN)};

    (3)2<qˉp<p<2, c>0 and μ(˜βN)<μ<0;

    then we can obtain the following results:

    (i) for N=4, system (PN) has a positive, radially symmetric solution (v,a1d) if d(0,1), and has no nontrivial solution if d[1,+);

    (ii) for N=6,8, system (PN) has two positive, radially symmetric solutions (v,˜β1N) and (v,˜β2N) if d(0,dN), has one positive, radially symmetric solution (v,12d) if d=dN, and has no nontrivial solution if d(dN,+), where dN=14a when N=6 and dN=427a2 when N=8.

    Next, in the Sobolev critical case 2<q<p=2, we have the following result.

    Proposition 3.2. Suppose that c>0 and 2<q<p=2 hold. Let ˜βN and d be defined by (1.5) and (1.6), respectively. If one of the following conditions holds:

    (1)2<q<ˉp<p=2 and 0<μ<min{ˇμ(˜βN),ˆμ(˜βN)};

    (2)ˉp=q<p=2 and 0<μ<ˉμ(˜βN);

    (3)ˉp<q<p=2 and 0<μ<μ(˜βN);

    then we have the following results:

    (i) for N=4, system (PN) has a positive, radially symmetric solution (v,a1d) if d(0,1), and has no nontrivial solution if d[1,+).

    (ii) for N=6,8, system (PN) has two positive, radially symmetric solutions (v,˜β1N) and (v,˜β2N) if d(0,dN), has one positive, radially symmetric solution (v,12d) if d=dN, and has no nontrivial solution if d(dN,+), where dN=14a when N=6 and dN=427a2 when N=8.

    In order to obtain conclusions of Proposition 3.1 and 2, we first recall some results about existence of normalized solutions for the equation (1.7).

    Theorem 3.1. [13] Suppose that c,˜βN>0, N4 and 2<q<p<2 hold. If one of the following conditions holds:

    (1)2<q<p=ˉp, 0<c<ˉCN(˜βN) and μ>0;

    (2)2<qˉp<p<2, c>0 and 0<μ<min{μ(˜βN),μ(˜βN)};

    (3)2<qˉp<p<2, c>0 and μ(˜βN)<μ<0;

    then Eq (1.7) has at least one positive, radially symmetric solution.

    Theorem 3.2. [14,Theorem 1.1] Suppose that c,˜βN>0, N4 and 2<q<p=2 hold. If one of the following conditions holds:

    (1)2<q<ˉp<p=2 and 0<μ<min{ˇμ(˜βN),ˆμ(˜βN)};

    (2)ˉp=q<p=2 and 0<μ<ˉμ(˜βN);

    (3)ˉp<q<p=2 and 0<μ<μ(˜βN);

    then Eq (1.7) has at least one positive, radially symmetric solution.

    Remark 3.1.

    (i) Theorem 3.1 can be obtained from [13] with some minor modifications. Specifically, (i), (ii) and (iii) of Theorem 3.1 can be derived from Theorems 1.1, 1.3 and 1.6, 1.9 of [13], respectively.

    (ii) In dimension N=4, the right hand side of the condition (1.6) in [14] is positive infinity. In dimension N5, instead, the value is finit. For more detail, we refer to the formula (6.1) in [14].

    Proof of Proposition 3.1. By Theorem 3.1, equation (1.7) has at least one positive, radially symmetric solution v when the assumptions in any case are satisfied. We define the function fv(β) as

    fv(β)=βabβN22v2L2(RN). (3.1)

    Case 1: N=4. In this case, by (1.6), (3.1), we have

    fv(β)=(1d)βa.

    It follows that if d(0,1), then

    ˜β4=a1d,

    defined as in (1.5), is a solution of fv(β)=0, and (v,˜β4) is a solution of (PN). If d[1,+), (PN) has no nontrivial solution. This implies that Proposition 3.1 (i) holds.

    Case 2: N=6. In this case, by (1.6), (3.1), we have

    fv(β)=dβ2+βa.

    Then it is easy to know that limβ+fv(β)= for any a,b>0 and fv(β)<0 for any β(0,a]. If d(0,14a), then there exist

    ˜β6,1=114ad2d(a,12d),

    and

    ˜β6,2=1+14ad2d(12d,+),

    such that (v,˜β6,1), (v,˜β6,2) solve (PN). If d=14a, then (v,12d) solves (PN). If d(14d,+), then (PN) has no nontrivial solution. Thus, we can obtain that Proposition 3.1 (ii) holds.

    Case 3: N=8. In this case, by (1.6), (3.1), we have

    fv(β)=dβ3+βa.

    Then it is easy to know that limβ+fv(β)= for any a,b>0 and fv(β)<0 for any β(0,a]. For each v0, we have

    ddβfv(β)=13dβ2.

    It is easy to see that fv(β) has a unique maximum point βv=13d>0, and

    maxβR+fv(β)=fv(βv)=233da.

    If d(0,427a2), it follows from Lemma 2.3 that the equation fv(β)=0 has three distinct real solutions

    ˜β8,j=23dcos(θ+2jπ3)(j=0,1,2),

    where

    θ=13arccos(3a23d). (3.2)

    It is easy to see that one of which is negative and two of which are positive. Due to β>0, we next wish to exclude the negative solution. Note that (3.2) and d(0,427a2), it follows that

    3a23d(1,0),

    and then

    θ(π6,π3).

    This leads to ˜β8,0,˜β8,2>0 and ˜β8,1<0. Moreover, it is easy to see that ˜β8,0>˜β8,2. Therefore, we conclude that if d(0,427a2), then there exist

    ˜β8,0=23dcosθ(13d,1d),

    and

    ˜β8,2=23dcos(θ+4π3)(a,13d),

    such that (v,˜β8,0), (v,˜β8,2) solve (PN). If d=427a2, the equation fv(β)=0 has a unique positive real solution

    ˜β8=13d,

    then (v,˜β8) solves (PN). If d(427a2,+), then (PN) has no nontrivial solution. Thus, we can obtain that Proposition 3.1 (iii) hold.

    Proof of Proposition 3.2. Similar to the proof of Proposition 3.1, and applying Theorem 3.2, we can prove Proposition 3.2.

    Proof of Theorem 1.1. Firstly, it is clear that u and v have the same sign and radial symmetry. We next prove the equivalence.

    Necessary condition. Suppose the equation (K) has a normalized solution uS(c), we then have

    (a+bu2L2(RN))RNuφdx+λRNuφdx=μRN|u|q2uφdx+RN|u|p2uφdx,φH1(RN).

    Let β=a+bu2L2(RN) and v(x)=u(β12x)=u(y), we conclude that

    β=a+bβN22v2L2(RN). (4.1)

    Let ˜βN be defined by (1.5), i.e.,

    ˜βN={a1d,N=4,1±14ad2d,N=6,23dcos(θ+2jπ3)(j=0,2),N=8,

    where d=bv2L2(RN), by a similar argument as used in the proof of Proposition 3.1, we obtain that β=˜βN>0 can solve (4.1) in dimensions N=4,6,8. Furthermore, let ϕ(x)=φ(β12x)=φ(y), we have

    RNv(x)ϕ(x)dx+λRNv(x)ϕ(x)dx=˜βN2N(˜βNRNu(y)φ(y)dy+λRNu(y)φ(y)dy)=˜βN2N(a+bRN|u(y)|2dy)RNu(y)φ(y)dy+λ˜βN2NRNu(y)φ(y)dy=˜βN2NμRN|u(y)|q2u(y)φ(y)dy+˜βN2NRN|u(y)|p2u(y)φ(y)dy=μRN|v(x)|q2v(x)ϕ(x)dx+RN|v(x)|p2v(x)ϕ(x)dx.

    Moreover, RN|v|2dx=˜βN2NRN|u(y)|2dy=˜βN2Nc2, which implies that (v,˜βN)S(˜βN4Nc)×R+ is a solution of (PN).

    Sufficient condition. Suppose the system (PN) has a solution (v,˜βN)S(˜βN4Nc)×R+. At least in a weak sense, we then have, in dimensions N=4,6,8,

    RNvϕdx+λRNvϕdx=μRN|v|q2vϕdx+RN|v|p2vϕdx,ϕH1(RN),

    and

    ˜βN=a+b˜βN22NRN|v|2dx.

    Let u(x)=v(˜β12Nx)=v(y) and φ(x)=ϕ(˜β12Nx)=ϕ(y) we can obtain that

    (a+bRN|u(x)|2dx)RNu(x)φ(x)dx+λRNu(x)φ(x)dx=˜βN2N(˜β1N(a+b˜βN22NRN|v(y)|2dy)RNv(y)ϕ(y)dy+λRNv(y)ϕ(y)dy)=˜βN2N(RNv(y)ϕ(y)dy+λRNv(y)ϕ(y)dy)=˜βN2N(μRN|v(y)|q2v(y)ϕ(y)dy+RN|v(y)|p2v(y)ϕ(y)dy)=μRN|u(x)|q2u(x)φ(x)dx+RN|u(x)|p2u(x)φ(x)dx,

    and

    RN|u|2dx=˜βN2NRN|v(y)|2dy=c2,

    which implies that uS(c) is a solution of (K).

    Proof of Theorem 1.2. The conclusions follow directly from Theorem 1.1 and Proposition 3.1.

    Proof of Theorem 1.3. The conclusions follow directly from Theorem 1.1 and Proposition 3.2.

    Proof of Theorem 1.4. Suppose that uS(c) is a positive solution of (K) with p=q=2, then

    au2L2(RN)+bu4L2(RN)+λu2L2(RN)=μuqLq(RN)+upLp(RN).

    It follows from Lemma 2.2 and uS(c) that

    λc2=μ(1γq)uqLq(RN)+(1γp)upLp(RN).

    Since p=q=2, we have γp=γq=1, it follows that λc2=0. However, by Lemma 2.1, we have λ>0, which is a contradiction. Hence, equation (K) has no positive solutions uS(c) for any μ>0.

    This work is supported by National Natural Science Foundation of China (No. 12071486).

    The authors declare there is no conflicts of interest.


    Acknowledgments



    Phu-Tho Nguyen is a graduate student researcher of Graduate University of Sciences and Technology, Vietnam Academy of Science and Technology, Vietnam. The authors would like to acknowledge the Science and Technology Program for the Southwestern Sustainable Development, Vietnam National University Ho Chi Minh City for the financial support. This publication's contents and interpretations are the sole responsibility of the authors.

    Conflicts of interest



    The authors declare no conflict of interest.

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