Degradation of poly aromatic fractions of crude oil and detection of catabolic genes in hydrocarbon-degrading bacteria isolated from Agbabu bitumen sediments in Ondo State

Temitayo O. Olowomofe; J. O. Oluyege; B.I. Aderiye; O. A. Oluwole; Temitayo O. Olowomofe; J. O. Oluyege; B.I. Aderiye; O. A. Oluwole

doi:10.3934/microbiol.2019.4.308

AIMS Microbiology

2019, Volume 5, Issue 4: 308-323. doi: 10.3934/microbiol.2019.4.308

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Research article

Degradation of poly aromatic fractions of crude oil and detection of catabolic genes in hydrocarbon-degrading bacteria isolated from Agbabu bitumen sediments in Ondo State

1.
Department of Microbiology, Ekiti State University, Ado-Ekiti
2.
Department of Science and Laboratory Technology, Ekiti State University, Ado-Ekiti

Received: 17 June 2019 Accepted: 09 September 2019 Published: 22 October 2019

Pollution due to release of Poly aromatic hydrocarbons (PAHs) are a major environmental issue especially in oil producing communities. This study investigates the polyaromatic hydrocarbon degradation potentials of some bacteria: Campylobacter hominis, Bacillus cereus, Dyadobacter koreensis, Pseudomonas aeruginosa and Micrococcus luteus isolated from Agbabu bitumen sediments in Ondo State. The isolates were used singly and in consortium for the degradation of Bonny light crude oil. Concentrations of residual aromatic hydrocarbons in crude oil degraded by these isolates were determined by Gas chromatography/Mass Spectroscopy with flame ionization detector (FID). Detection of catabolic genes (nahH, CatA and AlkB) in the isolates was determined by PCR amplification of their specific primers. The GC-MS analyses showed degradation of poly aromatic hydrocarbons (PAHs) by these isolates. The consortium exhibited the highest PAH reduction (73%) while C. hominis had the least PAH reduction (56%). Dyadobacter koreensis, P. aeruginosa, Micrococcus luteus and B. cereus, displayed 66%, 60%, 59% and 58% PAH reduction respectively. The catabolic gene nahH gene was present in B. cereus, D. koreensis, P. aeruginosa and M. luteus, alkB gene was present in B. cereus, C. hominis, and D. koreensis while CatA was not detected in any of the isolates. The findings of this study affirmed the hydrocarbon-degrading abilities and presence of catabolic genes in these bacteria, these make them potential tools in oil prospecting and cleaning up of hydrocarbon contaminated sites.

Keywords:

Citation: Temitayo O. Olowomofe, J. O. Oluyege, B.I. Aderiye, O. A. Oluwole. Degradation of poly aromatic fractions of crude oil and detection of catabolic genes in hydrocarbon-degrading bacteria isolated from Agbabu bitumen sediments in Ondo State[J]. AIMS Microbiology, 2019, 5(4): 308-323. doi: 10.3934/microbiol.2019.4.308

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Abstract

Acknowledgments

We are grateful to Ekiti State government for the funds given to us through the Ekiti State Scholarship Board Scheme to finance the project.

Conflict of interest

The authors declare that there is no conflict of interests regarding the publication of this article.

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