Citation: Constance Jeffery. Intracellular proteins moonlighting as bacterial adhesion factors[J]. AIMS Microbiology, 2018, 4(2): 362-376. doi: 10.3934/microbiol.2018.2.362
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Bacterial adherence factors, also known as adhesins, are proteins on the cell surface that form and maintain physical interactions with host cells and tissues. They are important in both health and disease as they are needed by pathogens for infection and by commensal or “good” bacteria to maintain a symbiotic relationship with the host. Surprisingly, several dozen of these proteins were previously identified as ubiquitous intracellular enzymes that have a canonical function in essential cellular processes and are sometimes referred to as “housekeeping enzymes” [1,2,3,4,5]. The first intracellular/surface moonlighting protein (ISMP) to be identified was an enzyme in glycolysis, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which has a second role on the surface of pathogenic streptococci [6]. Other intracellular/surface moonlighting proteins include other metabolic enzymes that are also widespread in evolution and function in glycolysis, the citric acid cycle, or DNA and protein metabolism, for example, phosphoglycerate kinase and enolase. Intracellular chaperones (Hsp60/GroEL, Hsp70/DnaK), and protein synthesis elongation factors (EF-Tu, EF-G) have also been found to serve as adhesins in bacteria (Table 1).
In general, moonlighting proteins comprise a subset of multifunctional proteins that perform two or more distinct and physiologically relevant biochemical or biophysical functions that are not due to gene fusions, multiple RNA splice variants, or pleiotropic effects [1]. The MoonProt Database includes information about hundreds of moonlighting proteins for which biochemical or biophysical evidence supports the presence of at least two biochemical functions in one polypeptide chain [7]. Of these, over 30 types of proteins have one function inside the cell and another function as an adhesin on the cell surface. Some are found to moonlight on the surface of multiple species, so there are over 100 ISMPs. The bacterial ISMPs (Table 1) are found in typical Gram-positive and Gram-negative species, as well as mycobacteria, spirochetes, and mycoplasma.
An ISMP can have different extracellular functions in different species. Enolase converts the reversible conversion of 2-phosphoglycerate to phosphoenolpyruvate in the cytoplasm in glycolysis and gluconeogenesis and has been found to have many moonlighting functions in addition to being an adhesin on the bacterial cell surface. As a bacterial adhesin, enolase binds host proteins in the extracellular matrix, mucin, and other proteins and plays an important role in infection of mammalian and avian hosts [8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24] (Figure 1). Some ISMPs also have third (or more) functions as secreted soluble proteins, in many cases with roles in modulation of the immune system [2,3].
In pathogenic bacteria the extracellular function often plays a key role in infection or virulence [2,3]. ISMPs have been found to be involved in aiding the bacteria to bind directly to host cells, including fructose-1,6-bisphosphate aldolase from Neisseria meningitidis [25] and Streptococcus pneumoniae [26] and the Hsp60 chaperone from Clostridium difficile [27], Helicobacter pylori [28], Chlamydia pneumoniae [29], Legionella pneumophila [30] and several other species. In some cases a specific receptor on the host cell surface has been identified. Listeria monocytogenes alcohol acetaldehyde dehydrogenase binds to Hsp60 (another moonlighting protein) on the surface of several human cell lines [31,32]. Streptococcus pyogenes GAPDH binds to the uPAR/CD87 receptor [33]. Streptococcus pneumoniae fructose 1,6-bisphosphate aldolase binds to the flamingo cadherin receptor (FCR) [26]. Haemophilus ducreyi Hsp60 binds to membrane glycosphingolipids [34,35].
Other ISMPs bind to extracellular matrix or secreted mucins in the mucosal layer of the intestines and airway. Mycoplasma pneumoniae EF-Tu and pyruvate dehydrogenase, Mycobacterium tuberculosis malate synthase, and Streptococcus mutans autolysin AltA, Staphylococcus caprae autolysin AltC, and Staphylococcus aureus autolysin Aaa bind to one or more of the extracellular matrix components fibronectin, laminin, and/or collagen [36,37,38,39,40]. Mycoplasma genitalium GAPDH, Salmonella typhimurium Hsp60, and Streptococcus gordonii enolase, EF-Tu, and the beta subunit of the DNA-directed RNA polymerase bind mucin [18,41,42]. Other examples are given in Table 1.
Figure 1. Intracellular enzymes and chaperones can function as adhesins on the bacterial cell surface. An ISMP can function as an enzyme inside of the cell and an adhesin when located on the cell surface. Enolase is found in the cytoplasm in almost all species where it converts 2-phosphoglycerate to phosphoenolpyruvate as the ninth step of glycolysis. In many species of bacteria, it is also found on the cell surface where it can bind to the host's extracellular matrix or airway mucins. For pathogenic bacteria, this attachment can be important for invading host tissues and promoting infection. In most cases, how the intracellular enzyme is transported outside the cell and how it becomes attached to the cell surface are not known (curved arrow). There might be a receptor for the protein on the bacterial cell surface (hexagon), but the nature of the surface attachment is known for only a few ISMPs.The ability of many pathogenic bacteria to use surface proteins to bind to the soluble host protein plasminogen also assists in invasion of host tissues [11,12,13,14,43,44]. Plasminogen is a precursor to plasmin, which is a broad-spectrum serine protease present in blood that helps break down fibrin clots [45]. When an invading pathogen uses a receptor on its surface to bind plasminogen from the host, the plasminogen can be converted to plasmin, the active form of the protease, by using an endogenous protease or subverting the host's tissue-type plasminogen (tPA) activators and urokinase-type plasminogen activators [46]. The active plasmin that is then attached to the surface of the invading organism can be used as a general protease to degrade host extracellular matrix and basement membrane, thereby facilitating migration through tissues. In the case of Mycoplasma hyopenumoniae, a swine-specific pathogen with a reduced genome that lacks genes for building amino acids, having an active protease on the surface enables cleavage of a variety of host proteins to produce peptides and amino acids that can be taken up by the bacterium as nutrients [47,48]. Other ISMPs also aid in infection and virulence by serving as receptors on the bacterial cell surface to acquire nutrients from the host. Staphylococcal GAPDH serves as a transferrin binding protein to acquire iron from the host [49].
The use of moonlighting proteins in adherence to host cells and tissues is not seen only in pathogenic species. Bacterial species that are sometimes referred to as “good” bacteria or probiotics, in other words nonpathogenic symbionts that help promote health and well-being, use ISMP in commensal interactions with host species, especially in the intestines. Lactobacillus plantarum GAPDH and enolase were shown to aid the bacterium in binding to mammalian cells and could play a role for this probiotic species to bind to the lining of the gut [23,50]. Lactobacillus johnsonii EF-Tu and Hsp60 also bind to human cells and to mucin [51,52]. Lactobacillus acidophilus GAPDH also binds mucin [53].
ISMPs may also assist in symbiotic relationships with other species, including a symbiotic relationship between lactic acid bacteria and yeast. The bacteria break down starch and other carbohydrates to produce lactic acid that is used by the yeast. In return they receive nutrients made by the yeast. This symbiotic relationship is found in several kinds of fermented foods like kefir, a drink made from cow's milk. Lactococcus lactis GAPDH, pyruvate kinase, Hsp60/GroEL, DnaK/Hsp70, and 6-phosphofructokinase have been shown to bind to invertase on the surface of the yeast Saccharomyces cerevisiae to help maintain this inter-species symbiotic interaction [54].
One of the benefits of probiotic bacteria has been suggested to be that they compete with pathogens for binding sites or nonspecific binding to the surface of epithelial cells lining the gut. Several moonlighting proteins were found to aid Lactobacillus species in competing with pathogenic species for binding to epithelial cell lines in vitro. Some of the same ISMPs may be involved in the competition of pathogenic and commensal bacteria for binding to epithelial cells. Several of the moonlighting proteins have been found to perform the same combination of enzyme and adhesion functions in both pathogenic and commensal bacteria, for example, enolases from Lactobacillus, Staphylococcus, Streptococcus and several other species bind human plasminogen [14].
The adhesion functions of the ISMPs in Table 1 were mainly found through experiments to identify proteins that bind to a specific molecular target, such as collagen, fibronectin, or other extracellular matrix proteins or through studies of proteins involved in binding to a specific target cell type. In recent years, many more intracellular proteins have been found to have a second location on the cell surface through surface proteomics, or “surfomics”, studies that aimed to identify all the proteins on a cell surface [55]. Surface proteomics studies employ variations of three types of experimental approaches to identify cell surface proteins. The main difference in the methods is in how the candidate proteins are isolated: through fractionating the cells to isolate components of the cell membrane and/or cell wall, surface “shaving” or using proteases to digest proteins on the cell surface without damaging the cell membrane, or labeling proteins on the surface with biotin or O18 before disrupting the cells and isolating the proteins. In each case the surface proteins are then identified using mass spectrometry. Although these methods might incorrectly identify some strictly intracellular proteins as being part of the cell surface proteome due to experimental artifacts inherent in the challenges of cell fractionation, and even some intracellular proteins that are correctly found to have a second location on the cell surface might have a different function other than as adhesins, at least some of the known intracellular/surface adhesins were correctly found to be localized to the cell surface, and it is possible that some of the additional cytoplasmic proteins found in these studies are also moonlighting as adhesins. Additional experiments are needed to determine if the intracellular proteins identified as being on the cell surface through proteomics methods are indeed involved in bacterial adhesion and were not found on the surface because they have another role on the surface or perhaps they were artifacts of the experimental methods.
It might at first seem unlikely that so many intracellular chaperones and enzymes required for central metabolism evolved to function also as cell surface binding proteins. Acquiring the new function required (1) evolution of a new protein-protein interaction site as well as (2) mechanisms for secretion and cell surface attachment, all while maintaining the first function of the protein. Satisfying the first requirement can be surprisingly simple. In general, most of the amino acid residues on a protein's surface are not directly connected to the protein's main function and are therefore not under significant selective pressure during evolution. In fact, surface amino acids vary a great deal even among close homologues. Having just a small number of these surface residues in a correct three-dimensional arrangement can be sufficient for formation of a novel protein-protein interaction site. In fact, Ehinger and coworkers showed that a nine amino acid sequence on the surface of enolase was sufficient for its interaction with plasminogen [56]. In general, for an average protein comprised of 300 or 400 amino acids, there is ample space and material for development of a new protein-protein binding site. In addition, most of these proteins are essential housekeeping proteins that first evolved billions of years ago and are expressed in many species and cell types, providing both the time and variety of cellular conditions for evolution of the protein surface to include a new binding function.
A more difficult question is how most of the ISMPs are secreted and become attached to the cell surface. The ISMPs do not contain a signal sequence or the twin arginine motif found in most proteins secreted by the canonical Sec or TAT secretion systems, respectively. For these reasons, the ISMPs are sometimes referred to as anchorless surface proteins or surface-associated housekeeping enzymes and are said to be secreted through non-classical, noncanonical, or unconventional secretion pathways. It is not clear if any of the known non-canonical secretion systems are involved in the secretion of ICMS, but most still require a kind of secretion signal, and they tend to be involved in the secretion of a few specific proteins [57].
Although it has been suggested that these intracellular proteins could become released from dead or damaged cells, several lines of evidence support the idea that at least some of them do require a secretion system [58,59]. First, the ISMPs are not the most abundant proteins in the cell, and those proteins that are most abundant are not often found on the cell surface. Second, a large portion of the pool of each protein type remains inside the cell while only some of the pool of the protein is partitioned to the cell surface. Why only part of the cytoplasmic pool of these specific proteins become targeted to the cell surface is not known.
For some individual proteins, there is additional evidence that a secretion system is probably involved. Yang and coworkers concluded that the release of GroEL, DnaK, enolase, pyruvate dehydrogenase subunits PdhB and PdhD, and superoxide dismutase SodA, by Bacillus subtilis is not due to gross cell lysis based on observing a constant cell density, no change in secretion in the presence of chloramphenicol, constant cell viability count, negligible amounts of two highly expressed cytoplasmic proteins EF-Tu and SecA in the culture medium, and the lack of effect of deleting lytC and lytD autolysins on the amount of the proteins in the media [60]. They also showed that these proteins were not released into the medium by membrane vesicles and there was no N-terminal cleavage (which might have suggested the presence of a signal sequence). Also, a mutant form of enolase with a hydrophobic helix replaced with a more neutral helix was retained in the cell when the wild type protein was found in the media, which also supports the model that it is not due to cell lysis. They followed up by showing that in Bacillus subtilis enolase, the internal hydrophobic helical domain was essential but not sufficient for export of enolase [61], although a larger portion of the N-terminal domain (residues 1-140) was sufficient for export of GFP in B subtilis and E coli. Boel and colleagues found that Lys341 of E. coli, Enterococcus faecalis, and Bacillus subtilis enolase becomes spontaneously modified with the substrate 2-phosphoglycerate (2PG), and this post-translational modification is required for export form the cell [62]. Substitution of Lys341 with other amino acids (Ala, Arg, Glu, Gln) prevented modification and secretion even though the Lys341Glu mutant enzyme was enzymatically active, showing that enzyme activity was not sufficient for secretion (and also that secretion was not due to cell leakage, because a single amino acid change can cause a decrease in secretion).
Secretion of some ISMPs may involve an as yet unknown secretion pathway, or their secretion might utilize an alternative version of one or more of the known secretion systems. If the latter is true, there are several possibilities, which are not mutually exclusive: One or more of the known secretion systems could be leaky. Post translational modifications (PTMs), possibly transient PTMs, can render some subset of the ISMP to be passable substrates. Alternative versions of the secretion systems might exist that require additional proteins such as a chaperone that have not yet been identified. An alternative system's secretion could be in competition with folding/unfolding or only rare conformations of an ISMP might be competent for secretion. It's possible that some combination of these factors could result in an inefficient secretion process, or that the alternative version requires induction of the expression of an unknown protein component of a known secretion system or an enzyme involved in adding PTMs. A search for shared characteristics might suggest what protein features singled out these intracellular proteins for adoption to play a second role on the cell surface, but a study of 98 ISMPs found that they share physical characteristics typical of intracellular proteins [63]. A couple studies have identified peptides on the cell surface that are the results of proteolytic cleavage of intracellular proteins, including EF-Tu [64,65], and the authors suggested that cleavage might yield peptides that are better at binding to some host proteins than the intact ISMPs. Because intact versions of these proteins are also found on the cell surface, the proteolytic cleavage is likely to take place after transport across the membrane and not as part of the secretion mechanism.
After the intracellular proteins are transported to the extracellular milieu, they become anchored to the surface of the bacterial cells, but in most cases, the mechanism for cell surface anchoring is also not known. For surface proteins in general, known anchoring mechanisms involve an N-terminal signal sequence for secretion and/or a C-terminal sorting motif, such as the LPXTG motif that is recognized by sortase A, for anchoring to the peptidoglycan network on the cell surface [66]. A smaller number of surface proteins have been found to be targeted to the cell surface due to the presence of additional motifs [67,68,69], including the GW repeat, the choline binding motif, and the LysM domain, but these are not found in the majority of the ISMPs in Table 1. Studies with purified proteins have shown that some intracellular/surface moonlighting proteins can adhere to the cell surface by re-association in both Gram-positive and Gram-negative bacteria, so it is possible that some of the ISMPs are secreted and then re-associate with the cell surface of after secretion. An increase in extracellular pH has been shown to cause some Lactobacillus crispatus ISMPs to be released from the cell surface [70]. Some ISMPs may also be released from the surface during cell-wall renewal that occurs during exponential growth phase [71]. In most cases it is not known to which components of the cell surface—proteins, lipids, etc.—the proteins bind, but it was shown recently that extracellular enolase is bound to a rhamnose residue in cell membrane of mycoplasma [72], and enolase and GAPDH bind covalently to lipotectoic acid on Lactobacillus crispatus [73].
With the increasing problem of antibiotic resistance [74,75], new methods for inhibiting bacterial infections and virulence are needed, and studies of ISMPs might provide new targets for the development of novel therapeutics. But it's not the moonlighting proteins themselves that might be the best targets. The catalytic mechanisms of most of these ISMP are conserved between bacteria and their human hosts, which makes sense because they play key roles in central metabolic pathways such as glycolysis. Instead of targeting the ISMPs, elucidating how these proteins are targeted to the bacterial cell surface might identify processes and proteins that are involved in the novel secretion systems (or new versions of known secretion systems) or surface attachment mechanisms and that could serve as novel targets for developing new strategies for controlling infection.
Learning how pathogenic and commensal bacteria adhere to host cells and tissues could also lead to better understanding of how these species colonize host tissues and compete with each other. This information can be important in treatment of diseases that involve an imbalance of pathogenic and probiotic bacterial species, for example ulcerative colitis and Crohn's disease [76], which are autoimmune diseases of the gut that affect over a million people in the US alone [77]. Understanding bacterial adhesion could potentially lead to information about how probiotic species could be used to displace pathogens and improve the balance of bacterial species.
| Protein | Species | UniProt ID | Extracellular function | References |
| 6-phosphofructokinase | Lactococcus lactis | P0DOB5 | yeast invertase | [54] |
| Streptococcus oralis | E6KMA1 | plasminogen | [78] | |
| Aaa autolysin | Staphylococcus aureus | Q2YVT4 | fibronectin | [37] |
| Aae autolysin | Staphylococcus epidermis | Q8CPQ1 | fibrinogen, fibronectin, vitronectin | [79] |
| Aspartase | Haemophilus influenzae | P44324 | plasminogen | [80] |
| Atla autolysin | Streptococcus mutans | U3SW74 | fibronectin | [39] |
| AtlC autolysin | Staphylococcus caprae | Q9AIS0 | fibronectin | [40] |
| Bile salt hydrolase | Bifidobacterium lactis | Q9KK62 | plasminogen | [81] |
| C5a peptidase | Streptococcus agalactiae | Q8E4T9 | fibronectin | [82] |
| DNA-directed RNA polymerase beta subunit | Streptococcus gordonii | A0EKJ1 | Muc7 | [18] |
| DnaK | Bifidobacterium | Q8G6W1 | plasminogen | [81] |
| Lactococcus lactis | P0A3J0 | yeast invertase | [54] | |
| Mycobacterium tuberculosis | A0A0H3L5C8 | plasminogen | [75] | |
| Neisseria meningitidis | A9M296 | plasminogen | [20] | |
| EF-Tu | Lactobacillus johnsonii | Q74JU6 | cells, mucins | [51] |
| Mycoplasma pneumoniae | P23568 | fibronectin, epithelial cells, plasminogen, heparin, fetuin, actin, fibrinogen, vitronectin, laminin | [36,64] | |
| Pseudonomas aeruginosa | P09591 | plasminogen | [43] | |
| Streptococcus gordonii | A8AWA0 | Muc7 | [18] | |
| Elongation factor G | Streptococcus gordonii | A8AUR6 | Muc7 | [18] |
| Endopeptidase O | Streptococcus pneumoniae | Q8DNW9 | plasminogen, fibronectin | [83] |
| Enolase | Aeromonas hydrophila | Q8GE63 | plasminogen | [22] |
| Bacillus anthracis | D8H2L1 | plasminogen, laminin | [8] | |
| Bifidobacterium lactis | B7GTK2 | plasminogen | [11] | |
| Borrelia burgdorferi | B7J1R2 | plasminogen | [16] | |
| Lactobacillus crispatus | Q5K117 | plasminogen, laminin | [14] | |
| Lactobacillus johnsonii | Q74K78 | plasminogen, laminin | [14] | |
| Lactobacillus plantarum | Q88YH3 | fibronectin | [23] | |
| Leishmania mexicana | Q3HL75 | plasminogen | [13] | |
| Mycoplasma fermentans | C4XEI3 | plasminogen | [24] | |
| Mycoplasma suis | F0QRW4 | red blood cells | [84] | |
| Mycoplasma synoviae | Q4A740 | plasminogen, fibronectin | [9] | |
| Neisseria meningitidis | E0N8L2 | plasminogen | [20] | |
| Staphylococcus aureus | Q6GB54 | plasminogen, laminin | [14,21] | |
| Streptococcus canis | I7WI49 | plasminogen | [17] | |
| Streptococcus gordonii | A8AY46 | Muc7 | [18] | |
| Streptococcus mutans | Q8DTS9 | plasminogen | [12] | |
| Streptococcus oralis | A0A1F1EC06 | plasminogen | [19] | |
| Streptococcus pneumoniae | Q97QS2 | plasminogen | [14] | |
| Streptococcus pyogenes | Q1JML5 | plasminogen | [14] | |
| Streptococcus suis | A4W2T1 | fibronectin, plasminogen | [15] | |
| Fructose 1,6-bisphosphate aldolase | Neisseria meningitidis | F0N9L0 | cells | [25] |
| GAPDH | Bacillus anthracis | Q81X74 | plasminogen | [85] |
| Lactobacillus acidophilus | Q5FL51 | mucin | [53] | |
| Lactobacillus plantarum | F9UM10 | mucin, Caco-2 cells | [50] | |
| Lactococcus lactis | P52987 | yeast invertase | [54] | |
| Mycoplasma genitalium | P47543 | mucin | [41] | |
| Staphylococcus aureus | Q6GIL8 | transferrin | [49] | |
| Streptococcus agalactiae | Q9ALW2 | plasminogen | [86] | |
| Streptococcus oralis | A0A0F2E7M6 | plasminogen | [78] | |
| Streptococcus pneumoniae | A0A0H2US80 | plasminogen | [87] | |
| Streptococcus pyogenes | P68777 | uPAR/CD87 receptor on human cells, plasminogen | [33,88] | |
| Streptococcus suis | Q3Y454 | plasminogen | [89] | |
| Glucose 6-phosphate isomerase | Lactobacillus crispatus | K1MKZ7 | laminin, collagen | [90] |
| Glutamine synthetase | Lactobacillus crispatus | D5GYN9 | fibronectin, laminin, collagen I, plasminogen | [90] |
| Mycobacterium tuberculosis | A0A0H3LHU4 | plasminogen, fibronectin | [91] | |
| Bifidobacterium lactis | C2GUH0 | plasminogen | [81] | |
| Hsp60 | Chlamydiae pneumoniae | P31681 | adhesin | [29] |
| Lactococcus lactis | P37282 | yeast invertase | [54] | |
| Legionella pneumophila | Q5X762 | adhesin | [30] | |
| Clostridium difficile | Q9KKF0 | adhesin | [27] | |
| Haemophilus ducreyi | P31294 | glycosphinngolipids | [34,35] | |
| Helicobacter pylori | Q8RNU2 | adhesin | [28] | |
| Lactobacillus johnsonii | F7SCR2 | adhesin | [52] | |
| Listeria | Q8KP52 | adhesin | [32] | |
| Salmonella typhimurium | P0A1D3 | mucus | [42] | |
| Hsp65/Cpn60.2/GroEL2 | Mycobacterium tuberculosis | A0A0H3LCC3 | CD43 on macrophage surface | [92] |
| Leucyl aminopeptidase | Mycoplasma hyopneumoniae | Q4A9M4 | heparin | [93] |
| Malate synthase | Mycobacterium tuberculosis | P9WK17 | fibronectin, laminin, epithelial cells | [38] |
| Glutamyl aminopeptidase | Mycoplasma hyopneumoniae | Q4AAK4 | plasminogen, heparin | [47] |
| Leucyl aminopeptidase | Mycoplasma hyopneumoniae | Q4A9M4 | plasminogen, heparin, DNA | [48] |
| Ornithine carbamoyltransferase | Staphylococcus epidermidis | P0C0N1 | fibronectin | [94] |
| Peroxiredoxin | Neisseria meningitidis | A0A125WDU3 | plasminogen | [20] |
| Streptococcus agalactiae | E7S2A7 | heme | [95] | |
| Phosphoglycerate kinase | Streptococcus oralis | A0A0G7HBY7 | plasminogen | [77] |
| Streptococcus agalactiae | Q8DXT0 | plasminogen, actin | [83,96] | |
| Streptococcus pneumoniae | Q8DQX8 | plasminogen | [97] | |
| Phosphoglycerate mutase | Bifidobacterium lactis | P59159 | plasminogen | [81] |
| Streptococcus oralis | E6IYJ0 | plasminogen | [78] | |
| Pyruvate dehydrogenase | Mycoplasma pneumoniae | P75391 | fibrinogen | [36] |
| Pyruvate kinase | Lactococcus lactis | Q07637 | yeast invertase | [54] |
| Superoxide dismutase | Mycobacterium avium | P53647 | adhesin | [98] |
| Triose phosphate isomerase | Streptococcus oralis | E6J203 | plasminogen | [78] |
DownLoad: CSVThe large number of ISMPs, the variety of bacterial species, and the different host proteins targeted suggests that this phenomenon of intracellular housekeeping proteins moonlighting as adhesins on the bacterial cell surface is widespread. There is still a great deal to learn about these proteins, especially how these intracellular proteins are secreted and attached to the bacterial cell surface. Studies of ISMP that serve as adhesins could help in identifying novel targets for development of therapeutics because their mechanisms of secretion and membrane attachment are likely to involve new proteins and cellular processes.
The author declares no conflict of interest in this paper.
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| 20. | Yanina Lamberti, Kristin Surmann, The intracellular phase of extracellular respiratory tract bacterial pathogens and its role on pathogen-host interactions during infection, 2021, 34, 0951-7375, 197, 10.1097/QCO.0000000000000727 | |
| 21. | Wanderson Marques da Silva, Nubia Seyffert, Artur Silva, Vasco Azevedo, A journey through the Corynebacterium pseudotuberculosis proteome promotes insights into its functional genome, 2021, 9, 2167-8359, e12456, 10.7717/peerj.12456 | |
| 22. | Mahalingam Srinivasan, Subramanian Muthukumar, Durairaj Rajesh, Vinod Kumar, Rajamanickam Rajakumar, Mohammad Abdulkader Akbarsha, Balázs Gulyás, Parasuraman Padmanabhan, Govindaraju Archunan, The Exoproteome of Staphylococcus pasteuri Isolated from Cervical Mucus during the Estrus Phase in Water Buffalo (Bubalus bubalis), 2022, 12, 2218-273X, 450, 10.3390/biom12030450 | |
| 23. | A. Paula Domínguez Rubio, Cecilia L. D’Antoni, Mariana Piuri, Oscar E. Pérez, Probiotics, Their Extracellular Vesicles and Infectious Diseases, 2022, 13, 1664-302X, 10.3389/fmicb.2022.864720 | |
| 24. | Fei Hao, Xing Xie, Zhixin Feng, Rong Chen, Yanna Wei, Jin Liu, Qiyan Xiong, Guoqing Shao, Johnson Lin, NADH oxidase of Mycoplasma hyopneumoniae functions as a potential mediator of virulence, 2022, 18, 1746-6148, 10.1186/s12917-022-03230-7 | |
| 25. | Xia Liu, Ting Luan, Wanqing Zhou, Lina Yan, Hua Qian, Pengyuan Mao, Lisha Jiang, Jingyan Liu, Can Rui, Xinyan Wang, Ping Li, Xin Zeng, Denise Monack, The Role of 17β-Estrogen in Escherichia coli Adhesion on Human Vaginal Epithelial Cells via FAK Phosphorylation, 2021, 89, 0019-9567, 10.1128/IAI.00219-21 | |
| 26. | Pramod Yadav, Raja Singh, Souvik Sur, Sandhya Bansal, Uma Chaudhry, Vibha Tandon, Moonlighting proteins: beacon of hope in era of drug resistance in bacteria, 2023, 49, 1040-841X, 57, 10.1080/1040841X.2022.2036695 | |
| 27. | Teresa Requena, Gaspar Pérez Martínez, 2022, 9780128220368, 197, 10.1016/B978-0-12-819265-8.00094-2 | |
| 28. | Weng Yu Lai, Zhenpei Wong, Chiat Han Chang, Mohd Razip Samian, Nobumoto Watanabe, Aik-Hong Teh, Rahmah Noordin, Eugene Boon Beng Ong, Identifying Leptospira interrogans putative virulence factors with a yeast protein expression screen, 2022, 106, 0175-7598, 6567, 10.1007/s00253-022-12160-1 | |
| 29. | Cecile El-Chami, Rawshan Choudhury, Walaa Mohammedsaeed, Andrew J. McBain, Veera Kainulainen, Sarah Lebeer, Reetta Satokari, Catherine A. O’Neill, Multiple Proteins of Lacticaseibacillus rhamnosus GG Are Involved in the Protection of Keratinocytes From the Toxic Effects of Staphylococcus aureus, 2022, 13, 1664-302X, 10.3389/fmicb.2022.875542 | |
| 30. | Paola San-Martin-Galindo, Emil Rosqvist, Stiina Tolvanen, Ilkka Miettinen, Kirsi Savijoki, Tuula A. Nyman, Adyary Fallarero, Jouko Peltonen, Modulation of virulence factors of Staphylococcus aureus by nanostructured surfaces, 2021, 208, 02641275, 109879, 10.1016/j.matdes.2021.109879 | |
| 31. | Xing Xie, Fei Hao, Rong Chen, Jingjing Wang, Yanna Wei, Jin Liu, Haiyan Wang, Zhenzhen Zhang, Yun Bai, Guoqing Shao, Qiyan Xiong, Zhixin Feng, Nicotinamide Adenine Dinucleotide-Dependent Flavin Oxidoreductase of Mycoplasma hyopneumoniae Functions as a Potential Novel Virulence Factor and Not Only as a Metabolic Enzyme, 2021, 12, 1664-302X, 10.3389/fmicb.2021.747421 | |
| 32. | Olga S. Savinova, Olga A. Glazunova, Konstantin V. Moiseenko, Anna V. Begunova, Irina V. Rozhkova, Tatyana V. Fedorova, Exoproteome Analysis of Antagonistic Interactions between the Probiotic Bacteria Limosilactobacillus reuteri LR1 and Lacticaseibacillus rhamnosus F and Multidrug Resistant Strain of Klebsiella pneumonia, 2021, 22, 1422-0067, 10999, 10.3390/ijms222010999 | |
| 33. | Natalie A. Harrison, Christopher L. Gardner, Danilo R. da Silva, Claudio F. Gonzalez, Graciela L. Lorca, Identification of Biomarkers for Systemic Distribution of Nanovesicles From Lactobacillus johnsonii N6.2, 2021, 12, 1664-3224, 10.3389/fimmu.2021.723433 | |
| 34. | Keita Nishiyama, Cheng-Chung Yong, Nobuko Moritoki, Haruki Kitazawa, Toshitaka Odamaki, Jin-Zhong Xiao, Takao Mukai, Danilo Ercolini, Sharing of Moonlighting Proteins Mediates the Symbiotic Relationship among Intestinal Commensals, 2023, 0099-2240, 10.1128/aem.02190-22 | |
| 35. | Przemysław Sałański, Magdalena Kowalczyk, Jacek K. Bardowski, Agnieszka K. Szczepankowska, Health-Promoting Nature of Lactococcus lactis IBB109 and Lactococcus lactis IBB417 Strains Exhibiting Proliferation Inhibition and Stimulation of Interleukin-18 Expression in Colorectal Cancer Cells, 2022, 13, 1664-302X, 10.3389/fmicb.2022.822912 | |
| 36. | Jia Wang, Yao Li, Longji Pan, Jun Li, Yanfei Yu, Beibei Liu, Muhammad Zubair, Yanna Wei, Bala Pillay, Ademola Olufolahan Olaniran, Thamsanqa E. Chiliza, Guoqing Shao, Zhixin Feng, Qiyan Xiong, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation, 2021, 52, 1297-9716, 10.1186/s13567-021-00952-8 | |
| 37. | Tao Shi, Xi Guo, Yuqin Liu, Tingting Zhang, Xiangnan Wang, Zongjun Li, Yu Jiang, Rumen Metaproteomics Highlight the Unique Contributions of Microbe-Derived Extracellular and Intracellular Proteins for In Vitro Ruminal Fermentation, 2022, 8, 2311-5637, 394, 10.3390/fermentation8080394 | |
| 38. | Thomas B. Irving, Burcu Alptekin, Bailey Kleven, Jean‐Michel Ané, A critical review of 25 years of glomalin research: a better mechanical understanding and robust quantification techniques are required, 2021, 232, 0028-646X, 1572, 10.1111/nph.17713 | |
| 39. | Inés Reigada, Paola San-Martin-Galindo, Shella Gilbert-Girard, Jacopo Chiaro, Vincenzo Cerullo, Kirsi Savijoki, Tuula A. Nyman, Adyary Fallarero, Ilkka Miettinen, Surfaceome and Exoproteome Dynamics in Dual-Species Pseudomonas aeruginosa and Staphylococcus aureus Biofilms, 2021, 12, 1664-302X, 10.3389/fmicb.2021.672975 | |
| 40. | Jack A. Doolan, George T. Williams, Kira L. F. Hilton, Rajas Chaudhari, John S. Fossey, Benjamin T. Goult, Jennifer R. Hiscock, Advancements in antimicrobial nanoscale materials and self-assembling systems, 2022, 51, 0306-0012, 8696, 10.1039/D1CS00915J | |
| 41. | Yao Li, Jia Wang, Beibei Liu, Yanfei Yu, Ting Yuan, Yanna Wei, Yuan Gan, Jia Shao, Guoqing Shao, Zhixin Feng, Zhigang Tu, Qiyan Xiong, DnaK Functions as a Moonlighting Protein on the Surface of Mycoplasma hyorhinis Cells, 2022, 13, 1664-302X, 10.3389/fmicb.2022.842058 | |
| 42. | Yu Sun, Xuhang Wang, Qianwen Gong, Jin Li, Haosheng Huang, Feng Xue, Jianjun Dai, Fang Tang, Joanna B. Goldberg, Extraintestinal Pathogenic Escherichia coli Utilizes Surface-Located Elongation Factor G to Acquire Iron from Holo-Transferrin , 2022, 10, 2165-0497, 10.1128/spectrum.01662-21 | |
| 43. | Dorota Satala, Grzegorz Satala, Marcin Zawrotniak, Andrzej Kozik, Candida albicans and Candida glabrata triosephosphate isomerase – a moonlighting protein that can be exposed on the candidal cell surface and bind to human extracellular matrix proteins, 2021, 21, 1471-2180, 10.1186/s12866-021-02235-w | |
| 44. | Teresa Faddetta, Giovanni Renzone, Alberto Vassallo, Emilio Rimini, Giorgio Nasillo, Gianpiero Buscarino, Simonpietro Agnello, Mariano Licciardi, Luigi Botta, Andrea Scaloni, Antonio Palumbo Piccionello, Anna Maria Puglia, Giuseppe Gallo, Gladys Alexandre, Streptomyces coelicolor Vesicles: Many Molecules To Be Delivered, 2022, 88, 0099-2240, 10.1128/AEM.01881-21 | |
| 45. | Alain Filloux, Bacterial protein secretion systems: Game of types , 2022, 168, 1350-0872, 10.1099/mic.0.001193 | |
| 46. | Sébastien Massier, Brandon Robin, Marianne Mégroz, Amy Wright, Marina Harper, Brooke Hayes, Pascal Cosette, Isabelle Broutin, John D. Boyce, Emmanuelle Dé, Julie Hardouin, Phosphorylation of Extracellular Proteins in Acinetobacter baumannii in Sessile Mode of Growth, 2021, 12, 1664-302X, 10.3389/fmicb.2021.738780 | |
| 47. | Ana Luísa Matos, Pedro Curto, Isaura Simões, Moonlighting in Rickettsiales: Expanding Virulence Landscape, 2022, 7, 2414-6366, 32, 10.3390/tropicalmed7020032 | |
| 48. | Atsushi Kurata, Shimpei Takeuchi, Ryo Fujiwara, Kento Tamura, Tomoya Imai, Shino Yamasaki-Yashiki, Hiroki Onuma, Yasuhisa Fukuta, Norifumi Shirasaka, Koichi Uegaki, Activation of the toll-like receptor 2 signaling pathway by GAPDH from bacterial strain RD055328, 2023, 1347-6947, 10.1093/bbb/zbad059 | |
| 49. | Duoyi Hu, Irina Laczkovich, Michael J. Federle, Donald A. Morrison, Tina M. Henkin, Identification and Characterization of Negative Regulators of Rgg1518 Quorum Sensing in Streptococcus pneumoniae, 2023, 0021-9193, 10.1128/jb.00087-23 | |
| 50. | Jiah Yeom, Seongho Ma, Dong Joon Yim, Young-Hee Lim, Surface proteins of Propionibacterium freudenreichii MJ2 inhibit RANKL-induced osteoclast differentiation by lipocalin-2 upregulation and lipocalin-2-mediated NFATc1 inhibition, 2023, 13, 2045-2322, 10.1038/s41598-023-42944-y | |
| 51. | Ariana Casas-Román, María-José Lorite, Juan Sanjuán, María-Trinidad Gallegos, Two glyceraldehyde-3-phosphate dehydrogenases with distinctive roles in Pseudomonas syringae pv. tomato DC3000, 2024, 278, 09445013, 127530, 10.1016/j.micres.2023.127530 | |
| 52. | Dawei Chen, Congcong Guo, Chenyu Ren, Zihan Xia, Haiyan Xu, Hengxian Qu, Yunchao Wa, Chengran Guan, Chenchen Zhang, Jianya Qian, Ruixia Gu, Screening of Lactiplantibacillus plantarum 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying, 2023, 12, 2304-8158, 3604, 10.3390/foods12193604 | |
| 53. | Rivesh Maharajh, Manormoney Pillay, Sibusiso Senzani, A computational method for the prediction and functional analysis of potential Mycobacterium tuberculosis adhesin-related proteins , 2023, 1478-9450, 1, 10.1080/14789450.2023.2275678 | |
| 54. | Nicole J. Curtis, Krupa J. Patel, Amina Rizwan, Constance J. Jeffery, Moonlighting Proteins: Diverse Functions Found in Fungi, 2023, 9, 2309-608X, 1107, 10.3390/jof9111107 | |
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| 58. | Wenqian Liu, Zhen Wang, Shengjia Wang, Minghui Liu, Jian Zhang, Xuepeng Li, Hongye Wang, Jixing Feng, Identification of moonlighting adhesins of highly-adhesive Lactobacillus plantarum PO23 isolated from the intestine of Paralichthys olivaceus, 2024, 590, 00448486, 741044, 10.1016/j.aquaculture.2024.741044 | |
| 59. | Samuel A. Adeleye, Srujana S. Yadavalli, Jue D. Wang, Queuosine biosynthetic enzyme, QueE moonlights as a cell division regulator, 2024, 20, 1553-7404, e1011287, 10.1371/journal.pgen.1011287 | |
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Figures(1) / Tables(1)
Constance Jeffery. Intracellular proteins moonlighting as bacterial adhesion factors[J]. AIMS Microbiology, 2018, 4(2): 362-376. doi: 10.3934/microbiol.2018.2.362
| Protein | Species | UniProt ID | Extracellular function | References |
| 6-phosphofructokinase | Lactococcus lactis | P0DOB5 | yeast invertase | [54] |
| Streptococcus oralis | E6KMA1 | plasminogen | [78] | |
| Aaa autolysin | Staphylococcus aureus | Q2YVT4 | fibronectin | [37] |
| Aae autolysin | Staphylococcus epidermis | Q8CPQ1 | fibrinogen, fibronectin, vitronectin | [79] |
| Aspartase | Haemophilus influenzae | P44324 | plasminogen | [80] |
| Atla autolysin | Streptococcus mutans | U3SW74 | fibronectin | [39] |
| AtlC autolysin | Staphylococcus caprae | Q9AIS0 | fibronectin | [40] |
| Bile salt hydrolase | Bifidobacterium lactis | Q9KK62 | plasminogen | [81] |
| C5a peptidase | Streptococcus agalactiae | Q8E4T9 | fibronectin | [82] |
| DNA-directed RNA polymerase beta subunit | Streptococcus gordonii | A0EKJ1 | Muc7 | [18] |
| DnaK | Bifidobacterium | Q8G6W1 | plasminogen | [81] |
| Lactococcus lactis | P0A3J0 | yeast invertase | [54] | |
| Mycobacterium tuberculosis | A0A0H3L5C8 | plasminogen | [75] | |
| Neisseria meningitidis | A9M296 | plasminogen | [20] | |
| EF-Tu | Lactobacillus johnsonii | Q74JU6 | cells, mucins | [51] |
| Mycoplasma pneumoniae | P23568 | fibronectin, epithelial cells, plasminogen, heparin, fetuin, actin, fibrinogen, vitronectin, laminin | [36,64] | |
| Pseudonomas aeruginosa | P09591 | plasminogen | [43] | |
| Streptococcus gordonii | A8AWA0 | Muc7 | [18] | |
| Elongation factor G | Streptococcus gordonii | A8AUR6 | Muc7 | [18] |
| Endopeptidase O | Streptococcus pneumoniae | Q8DNW9 | plasminogen, fibronectin | [83] |
| Enolase | Aeromonas hydrophila | Q8GE63 | plasminogen | [22] |
| Bacillus anthracis | D8H2L1 | plasminogen, laminin | [8] | |
| Bifidobacterium lactis | B7GTK2 | plasminogen | [11] | |
| Borrelia burgdorferi | B7J1R2 | plasminogen | [16] | |
| Lactobacillus crispatus | Q5K117 | plasminogen, laminin | [14] | |
| Lactobacillus johnsonii | Q74K78 | plasminogen, laminin | [14] | |
| Lactobacillus plantarum | Q88YH3 | fibronectin | [23] | |
| Leishmania mexicana | Q3HL75 | plasminogen | [13] | |
| Mycoplasma fermentans | C4XEI3 | plasminogen | [24] | |
| Mycoplasma suis | F0QRW4 | red blood cells | [84] | |
| Mycoplasma synoviae | Q4A740 | plasminogen, fibronectin | [9] | |
| Neisseria meningitidis | E0N8L2 | plasminogen | [20] | |
| Staphylococcus aureus | Q6GB54 | plasminogen, laminin | [14,21] | |
| Streptococcus canis | I7WI49 | plasminogen | [17] | |
| Streptococcus gordonii | A8AY46 | Muc7 | [18] | |
| Streptococcus mutans | Q8DTS9 | plasminogen | [12] | |
| Streptococcus oralis | A0A1F1EC06 | plasminogen | [19] | |
| Streptococcus pneumoniae | Q97QS2 | plasminogen | [14] | |
| Streptococcus pyogenes | Q1JML5 | plasminogen | [14] | |
| Streptococcus suis | A4W2T1 | fibronectin, plasminogen | [15] | |
| Fructose 1,6-bisphosphate aldolase | Neisseria meningitidis | F0N9L0 | cells | [25] |
| GAPDH | Bacillus anthracis | Q81X74 | plasminogen | [85] |
| Lactobacillus acidophilus | Q5FL51 | mucin | [53] | |
| Lactobacillus plantarum | F9UM10 | mucin, Caco-2 cells | [50] | |
| Lactococcus lactis | P52987 | yeast invertase | [54] | |
| Mycoplasma genitalium | P47543 | mucin | [41] | |
| Staphylococcus aureus | Q6GIL8 | transferrin | [49] | |
| Streptococcus agalactiae | Q9ALW2 | plasminogen | [86] | |
| Streptococcus oralis | A0A0F2E7M6 | plasminogen | [78] | |
| Streptococcus pneumoniae | A0A0H2US80 | plasminogen | [87] | |
| Streptococcus pyogenes | P68777 | uPAR/CD87 receptor on human cells, plasminogen | [33,88] | |
| Streptococcus suis | Q3Y454 | plasminogen | [89] | |
| Glucose 6-phosphate isomerase | Lactobacillus crispatus | K1MKZ7 | laminin, collagen | [90] |
| Glutamine synthetase | Lactobacillus crispatus | D5GYN9 | fibronectin, laminin, collagen I, plasminogen | [90] |
| Mycobacterium tuberculosis | A0A0H3LHU4 | plasminogen, fibronectin | [91] | |
| Bifidobacterium lactis | C2GUH0 | plasminogen | [81] | |
| Hsp60 | Chlamydiae pneumoniae | P31681 | adhesin | [29] |
| Lactococcus lactis | P37282 | yeast invertase | [54] | |
| Legionella pneumophila | Q5X762 | adhesin | [30] | |
| Clostridium difficile | Q9KKF0 | adhesin | [27] | |
| Haemophilus ducreyi | P31294 | glycosphinngolipids | [34,35] | |
| Helicobacter pylori | Q8RNU2 | adhesin | [28] | |
| Lactobacillus johnsonii | F7SCR2 | adhesin | [52] | |
| Listeria | Q8KP52 | adhesin | [32] | |
| Salmonella typhimurium | P0A1D3 | mucus | [42] | |
| Hsp65/Cpn60.2/GroEL2 | Mycobacterium tuberculosis | A0A0H3LCC3 | CD43 on macrophage surface | [92] |
| Leucyl aminopeptidase | Mycoplasma hyopneumoniae | Q4A9M4 | heparin | [93] |
| Malate synthase | Mycobacterium tuberculosis | P9WK17 | fibronectin, laminin, epithelial cells | [38] |
| Glutamyl aminopeptidase | Mycoplasma hyopneumoniae | Q4AAK4 | plasminogen, heparin | [47] |
| Leucyl aminopeptidase | Mycoplasma hyopneumoniae | Q4A9M4 | plasminogen, heparin, DNA | [48] |
| Ornithine carbamoyltransferase | Staphylococcus epidermidis | P0C0N1 | fibronectin | [94] |
| Peroxiredoxin | Neisseria meningitidis | A0A125WDU3 | plasminogen | [20] |
| Streptococcus agalactiae | E7S2A7 | heme | [95] | |
| Phosphoglycerate kinase | Streptococcus oralis | A0A0G7HBY7 | plasminogen | [77] |
| Streptococcus agalactiae | Q8DXT0 | plasminogen, actin | [83,96] | |
| Streptococcus pneumoniae | Q8DQX8 | plasminogen | [97] | |
| Phosphoglycerate mutase | Bifidobacterium lactis | P59159 | plasminogen | [81] |
| Streptococcus oralis | E6IYJ0 | plasminogen | [78] | |
| Pyruvate dehydrogenase | Mycoplasma pneumoniae | P75391 | fibrinogen | [36] |
| Pyruvate kinase | Lactococcus lactis | Q07637 | yeast invertase | [54] |
| Superoxide dismutase | Mycobacterium avium | P53647 | adhesin | [98] |
| Triose phosphate isomerase | Streptococcus oralis | E6J203 | plasminogen | [78] |
DownLoad: CSV| Protein | Species | UniProt ID | Extracellular function | References |
| 6-phosphofructokinase | Lactococcus lactis | P0DOB5 | yeast invertase | [54] |
| Streptococcus oralis | E6KMA1 | plasminogen | [78] | |
| Aaa autolysin | Staphylococcus aureus | Q2YVT4 | fibronectin | [37] |
| Aae autolysin | Staphylococcus epidermis | Q8CPQ1 | fibrinogen, fibronectin, vitronectin | [79] |
| Aspartase | Haemophilus influenzae | P44324 | plasminogen | [80] |
| Atla autolysin | Streptococcus mutans | U3SW74 | fibronectin | [39] |
| AtlC autolysin | Staphylococcus caprae | Q9AIS0 | fibronectin | [40] |
| Bile salt hydrolase | Bifidobacterium lactis | Q9KK62 | plasminogen | [81] |
| C5a peptidase | Streptococcus agalactiae | Q8E4T9 | fibronectin | [82] |
| DNA-directed RNA polymerase beta subunit | Streptococcus gordonii | A0EKJ1 | Muc7 | [18] |
| DnaK | Bifidobacterium | Q8G6W1 | plasminogen | [81] |
| Lactococcus lactis | P0A3J0 | yeast invertase | [54] | |
| Mycobacterium tuberculosis | A0A0H3L5C8 | plasminogen | [75] | |
| Neisseria meningitidis | A9M296 | plasminogen | [20] | |
| EF-Tu | Lactobacillus johnsonii | Q74JU6 | cells, mucins | [51] |
| Mycoplasma pneumoniae | P23568 | fibronectin, epithelial cells, plasminogen, heparin, fetuin, actin, fibrinogen, vitronectin, laminin | [36,64] | |
| Pseudonomas aeruginosa | P09591 | plasminogen | [43] | |
| Streptococcus gordonii | A8AWA0 | Muc7 | [18] | |
| Elongation factor G | Streptococcus gordonii | A8AUR6 | Muc7 | [18] |
| Endopeptidase O | Streptococcus pneumoniae | Q8DNW9 | plasminogen, fibronectin | [83] |
| Enolase | Aeromonas hydrophila | Q8GE63 | plasminogen | [22] |
| Bacillus anthracis | D8H2L1 | plasminogen, laminin | [8] | |
| Bifidobacterium lactis | B7GTK2 | plasminogen | [11] | |
| Borrelia burgdorferi | B7J1R2 | plasminogen | [16] | |
| Lactobacillus crispatus | Q5K117 | plasminogen, laminin | [14] | |
| Lactobacillus johnsonii | Q74K78 | plasminogen, laminin | [14] | |
| Lactobacillus plantarum | Q88YH3 | fibronectin | [23] | |
| Leishmania mexicana | Q3HL75 | plasminogen | [13] | |
| Mycoplasma fermentans | C4XEI3 | plasminogen | [24] | |
| Mycoplasma suis | F0QRW4 | red blood cells | [84] | |
| Mycoplasma synoviae | Q4A740 | plasminogen, fibronectin | [9] | |
| Neisseria meningitidis | E0N8L2 | plasminogen | [20] | |
| Staphylococcus aureus | Q6GB54 | plasminogen, laminin | [14,21] | |
| Streptococcus canis | I7WI49 | plasminogen | [17] | |
| Streptococcus gordonii | A8AY46 | Muc7 | [18] | |
| Streptococcus mutans | Q8DTS9 | plasminogen | [12] | |
| Streptococcus oralis | A0A1F1EC06 | plasminogen | [19] | |
| Streptococcus pneumoniae | Q97QS2 | plasminogen | [14] | |
| Streptococcus pyogenes | Q1JML5 | plasminogen | [14] | |
| Streptococcus suis | A4W2T1 | fibronectin, plasminogen | [15] | |
| Fructose 1,6-bisphosphate aldolase | Neisseria meningitidis | F0N9L0 | cells | [25] |
| GAPDH | Bacillus anthracis | Q81X74 | plasminogen | [85] |
| Lactobacillus acidophilus | Q5FL51 | mucin | [53] | |
| Lactobacillus plantarum | F9UM10 | mucin, Caco-2 cells | [50] | |
| Lactococcus lactis | P52987 | yeast invertase | [54] | |
| Mycoplasma genitalium | P47543 | mucin | [41] | |
| Staphylococcus aureus | Q6GIL8 | transferrin | [49] | |
| Streptococcus agalactiae | Q9ALW2 | plasminogen | [86] | |
| Streptococcus oralis | A0A0F2E7M6 | plasminogen | [78] | |
| Streptococcus pneumoniae | A0A0H2US80 | plasminogen | [87] | |
| Streptococcus pyogenes | P68777 | uPAR/CD87 receptor on human cells, plasminogen | [33,88] | |
| Streptococcus suis | Q3Y454 | plasminogen | [89] | |
| Glucose 6-phosphate isomerase | Lactobacillus crispatus | K1MKZ7 | laminin, collagen | [90] |
| Glutamine synthetase | Lactobacillus crispatus | D5GYN9 | fibronectin, laminin, collagen I, plasminogen | [90] |
| Mycobacterium tuberculosis | A0A0H3LHU4 | plasminogen, fibronectin | [91] | |
| Bifidobacterium lactis | C2GUH0 | plasminogen | [81] | |
| Hsp60 | Chlamydiae pneumoniae | P31681 | adhesin | [29] |
| Lactococcus lactis | P37282 | yeast invertase | [54] | |
| Legionella pneumophila | Q5X762 | adhesin | [30] | |
| Clostridium difficile | Q9KKF0 | adhesin | [27] | |
| Haemophilus ducreyi | P31294 | glycosphinngolipids | [34,35] | |
| Helicobacter pylori | Q8RNU2 | adhesin | [28] | |
| Lactobacillus johnsonii | F7SCR2 | adhesin | [52] | |
| Listeria | Q8KP52 | adhesin | [32] | |
| Salmonella typhimurium | P0A1D3 | mucus | [42] | |
| Hsp65/Cpn60.2/GroEL2 | Mycobacterium tuberculosis | A0A0H3LCC3 | CD43 on macrophage surface | [92] |
| Leucyl aminopeptidase | Mycoplasma hyopneumoniae | Q4A9M4 | heparin | [93] |
| Malate synthase | Mycobacterium tuberculosis | P9WK17 | fibronectin, laminin, epithelial cells | [38] |
| Glutamyl aminopeptidase | Mycoplasma hyopneumoniae | Q4AAK4 | plasminogen, heparin | [47] |
| Leucyl aminopeptidase | Mycoplasma hyopneumoniae | Q4A9M4 | plasminogen, heparin, DNA | [48] |
| Ornithine carbamoyltransferase | Staphylococcus epidermidis | P0C0N1 | fibronectin | [94] |
| Peroxiredoxin | Neisseria meningitidis | A0A125WDU3 | plasminogen | [20] |
| Streptococcus agalactiae | E7S2A7 | heme | [95] | |
| Phosphoglycerate kinase | Streptococcus oralis | A0A0G7HBY7 | plasminogen | [77] |
| Streptococcus agalactiae | Q8DXT0 | plasminogen, actin | [83,96] | |
| Streptococcus pneumoniae | Q8DQX8 | plasminogen | [97] | |
| Phosphoglycerate mutase | Bifidobacterium lactis | P59159 | plasminogen | [81] |
| Streptococcus oralis | E6IYJ0 | plasminogen | [78] | |
| Pyruvate dehydrogenase | Mycoplasma pneumoniae | P75391 | fibrinogen | [36] |
| Pyruvate kinase | Lactococcus lactis | Q07637 | yeast invertase | [54] |
| Superoxide dismutase | Mycobacterium avium | P53647 | adhesin | [98] |
| Triose phosphate isomerase | Streptococcus oralis | E6J203 | plasminogen | [78] |
