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Review

Advancing sustainable practices with Paenibacillus polymyxa: From soil health to medical applications and molecular engineering

  • Received: 17 March 2025 Revised: 10 May 2025 Accepted: 13 May 2025 Published: 19 May 2025
  • Paenibacillus polymyxa is a multifaceted bacterium with widespread applications in agriculture, environmental management, medicine, and industry. In agricultural settings, it plays a crucial role in soil enhancement, plant growth promotion, and natural pathogen control, reducing the need for chemical interventions. Additionally, P. polymyxa exhibits promising potential in medical applications by aiding in infection prevention and supporting gastrointestinal health. In the realm of environmental management, this bacterium contributes to pollution remediation through biodegradation processes. Industrially, P. polymyxa is involved in producing enzymes, biofertilizers, bioplastics, and platform chemicals, offering sustainable alternatives that underscore its importance in driving sustainability initiatives. Despite these valuable attributes, widespread utilization of bioresources derived from naturally occurring P. polymyxa has been hampered by limited genetic manipulation capabilities and tools. In this comprehensive analysis, we aimed to provide a thorough understanding of P. polymyxa's characteristics, genetic resources, and metabolic capabilities, while highlighting its potential as a versatile platform for protein expression, metabolic engineering, and synthetic biology. We delved into the diverse sustainable applications of P. polymyxa in these domains, emphasizing its benefits, challenges, and future outlook in advancing sustainable practices. Furthermore, we underscore the critical need for continued research and development of advanced engineering techniques and genetic editing technologies tailored specifically for this bacterium.

    Citation: Imen Zalila-Kolsi, Ray Al-Barazie. Advancing sustainable practices with Paenibacillus polymyxa: From soil health to medical applications and molecular engineering[J]. AIMS Microbiology, 2025, 11(2): 338-368. doi: 10.3934/microbiol.2025016

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  • Paenibacillus polymyxa is a multifaceted bacterium with widespread applications in agriculture, environmental management, medicine, and industry. In agricultural settings, it plays a crucial role in soil enhancement, plant growth promotion, and natural pathogen control, reducing the need for chemical interventions. Additionally, P. polymyxa exhibits promising potential in medical applications by aiding in infection prevention and supporting gastrointestinal health. In the realm of environmental management, this bacterium contributes to pollution remediation through biodegradation processes. Industrially, P. polymyxa is involved in producing enzymes, biofertilizers, bioplastics, and platform chemicals, offering sustainable alternatives that underscore its importance in driving sustainability initiatives. Despite these valuable attributes, widespread utilization of bioresources derived from naturally occurring P. polymyxa has been hampered by limited genetic manipulation capabilities and tools. In this comprehensive analysis, we aimed to provide a thorough understanding of P. polymyxa's characteristics, genetic resources, and metabolic capabilities, while highlighting its potential as a versatile platform for protein expression, metabolic engineering, and synthetic biology. We delved into the diverse sustainable applications of P. polymyxa in these domains, emphasizing its benefits, challenges, and future outlook in advancing sustainable practices. Furthermore, we underscore the critical need for continued research and development of advanced engineering techniques and genetic editing technologies tailored specifically for this bacterium.



    In many imaging applications, images inevitably contain noise. Most of the literatures deal with the reconstruction of images corrupted by additive Gaussian noise, for instance [1,2,3,4,5,6]. However, in many engineering applications the noise has an impulsive characteristic, which is different from Gaussian noise and cannot be modeled by Gaussian noise. Based on [7], a type of impulsive degradation is given by Cauchy noise, which follows Cauchy distribution and appears frequently in radar and sonar applications, atmospheric and underwater acoustic images, wireless communication systems. For more details, we refer to [8,9]. Recently, much attention has been paid to dealing with Cauchy noise and several approaches have been proposed. In [10], Chang et al. employed recursive Markov random field models to reconstruct images corrupted by Cauchy noise. Based on non-Gaussian distributions, Loza et al. [11] proposed a statistical approach in the wavelet domain. By combining statistical methods with denoising techniques, Wan et al. [12] developed a segmentation approach for RGB images corrupted by Cauchy noise. Sciacchitano et al. [13] proposed a total variation (TV)-based variational method for reconstructing images corrupted by Cauchy noise. The variational model in [13] (called as SDZ model) is

    minu BV(Ω)Ω|Du|+λ2(Ωlog(γ2+(uf)2)dx+ηΩ(uu0)2dx), (1.1)

    where Ω is a bounded connected domain in R2,  BV(Ω) is the space of functions of bounded variation, u BV(Ω) (for more details, see (2.1)) represents the restored image and γ>0 is the scale parameter of Cauchy distribution. In (1.1), λ is a positive number, which controls the trade-off between the TV regularization term and the fidelity term, u0 is the image obtained by applying the median filter [14] to the noisy image f, and η>0 is a penalty parameter. If 8ηγ21, the objective functional in (1.1) is strictly convex and its solution is unique. The term η||uu0||22 in (1.1) results in the solution being close to the median filter result, but the median filter does not always perform well as to Cauchy noise removal. In order to avoid this, in [15], the authors developed the the alternating direction method of multipliers (ADMM) to solve the following non-convex variational model (called as MDH model) directly

    minu BV(Ω)Ω|Du|+λ2Ωlog(γ2+(Kuf)2)dx, (1.2)

    where K represents a linear operator. As we know, solutions of variational problems with  TV regularization have many desirable properties, such as the feature of preserving sharp edges. However, these solutions are always accompanied by blocking artifacts due to the property of  BV space.

    In order to overcome blocking artifacts, we will employ TGV as a regularization term. In [16], Bredies et al. proposed the concept of TGV, and they applied TGV to mathematical imaging problems to overcome blocking artifacts. For more details of TGV, we refer interested readers to [17,18]. In order to overcome the defect of the median filter result, based on the proximal algorithm idea, we will use the term ||uz||22 to convexify the non-convex fidelity term Ωlog(γ2+(uf)2)dx. To simplify computing, for the TGV regularization term, we employ the proximal method. Based on these, we propose the following model

    minz BGV2α(Ω),uL2(Ω){ TGV2α(z)+λ(Ωlog(γ2+(uf)2)dx}. (1.3)

    with the constraint u=z. Meanwhile, we compare the proposed model (1.3) with the following model

    minz BGV2α(Ω),uL2(Ω){ TGV2α(u)+λ(Ωlog(γ2+(uf)2)dx+η2Ω(uu0)2dx)}, (1.4)

    where u0 is the image obtained by applying the median filter [14] to the noisy image. According to Table 1, the numerical results show that the proposed model (1.3) is better than the model (1.4). Compared with previous reports, the main novelty of our proposed approach has been condensed into the following points:

    Table 1.  SSIM and PSNR measures for different methods, γ=5.
    SSIM PSNR
    Image Noisy Model (1.4) Ours Noisy Model (1.4) Ours
    Montage 0.3230 0.9213 0.9312 19.14 28.70 30.25
    lena 0.5377 0.9252 0.9287 17.94 31.01 31.24
    Vehicle 0.5707 0.9278 0.9322 19.20 30.83 31.14
    Saturn 0.2080 0.8729 0.9125 19.04 36.01 36.49
    parrot 0.3999 0.8732 0.8757 19.08 28.41 29.28

     | Show Table
    DownLoad: CSV

    1. Compared with the BV regularization term, we employ the TGV regularization term which preserves the image structure and we prove that the proposed model admits a unique solution.

    2. Different from the constraint by applying the median filter, we use the constraint by applying the proximal approach and experiment results show better performance.

    3. The previous literature used ADMM algorithm but we employ non-expansive operator and the fixed point algorithm such that the convergence of the proposed algorithm is more efficiently proved.

    The next part is organized as below. We propose a new model and show the model has a unique solution in Section 2. In Section 3, we employ a minimization scheme to deal with the new model. We show the convergence of the proposed algorithm in Section 4. The performance of the new method is demonstrated by numerical results in Section 5. Some remarks are concluded in Section 6.

    Similar to [13], we propose a new non-convex TGV model for denoising Cauchy noise. For completeness, firstly, a review of BV space and TGV space is given. For more details on TGV models and Cauchy noise removal, we refer to [19,20,21,22].

    For convenience, we introduce the following notations. The function u BV(Ω) iff uL1(Ω) and its TV is finite, where TV of u is

    Ω|Du|=sup{Ωudivϕdx:ϕC0(Ω,R2),||ϕ||1}. (2.1)

    The space  BV(Ω) is a Banach space with the norm ||u|| BV(Ω)=||u||L1(Ω)+Ω|Du| [23,24].

    Throughout the paper, we denote the dimension by d, which is typically 2 or 3. For convenience, Ckc(Ω,Symk(Rd)) expresses the space of compactly supported symmetric tensor field, where Symk(Rd) represents the symmetric tensors space on Rd, which can be written as [16]

    Symk(Rd)={w:Rd××RdkR|w  is  multilinear  and  symmetric}. (2.2)

    The TGV of order k with positive weights α=(α0,α1,,αk1) is defined as [16]

    TGVkα(u)=supϕ{Ωu divk(ϕ)dx  |  ϕCkc(Ω,Symk(Rd)),|| divjϕ||αj,j=0,,k1}. (2.3)

    When k=1,α=1, Sym1(Rd)=Rd, TGV1α(u)=TV(u). When k=2, Sym2(Rd) represents all symmetric Sd×d matrices as follows, for ξSym2(Rd),

    ξ=(ξ11ξ1dξd1ξdd)

    for more details, we refer to [16]. In the following part, we mainly use second-order TGV as

    TGV2α(u)=supϕ{Ωudiv2(ϕ)dx  |  ϕC2c(Ω,Sym2(Rd)),||ϕ||α0,||divϕ||α1}, (2.4)

    where

    (divϕ)i=nj=1ϕijxj,    div2ϕ=ni,j=12ϕijxixj,  ||ϕ||=supxΩ(ni,j=1|ϕi,j|2)12,

    and

    || divϕ||= supxΩ(ni=1|(divϕ)i|2)12.

    Following the notation in [25], we define the discretized grid as

    Ωh={(ih,jh)|i,jN,1iN1,1jN2},

    for some positive N1,N2N, where h denotes the grid width and we take h=1 for convenience. For convenience, we define U,W,Z as

    U={u:ΩhR},W={u:ΩhR2},Z={u:ΩhR2×2}. (2.5)

    For simplicity, the TGV2α functional will be discretized by finite differences with step-size 1. Based on [16], TGV2α(u) can be reformulated as

    TGV2α(u)=minwW{α0||uw||1+α1||ε(w)||1}. (2.6)

    where w=(w1,w2)TW, ε(w)=12(w+Tw). The operators and ε, respectly, denote

    :  UW,  u=(+xu+yu),

    ε:  WZ,  ε(w)=(+xw112(+yw1++xw2)12(+yw1++xw2)+yw2),

     div:WU, divw=xw1+yw2,

     divh:  ZW,   divhz=(xz11+yz12xz21+yz22).

    For more details on the above discretion, we refer to [26].

    By [27], the Cauchy distribution can be written as

    P(x)=γπ((xμ)2+γ2), (2.7)

    where x represents a random variable which obeys the Cauchy distribution, μ represents the peak location, γ>0 is a scale parameter. The scale parameter is similar to the role of the variance. Here, we denote Cauchy distribution by C(μ,γ).

    Following [13], we denote random variables by f,u,v and the respective instances by f,u,v. Denote the noisy image by f=u+v, where v follows the Cauchy noise. We assume that the v follows the Cauchy distribution with μ=0 and its density function is defined as follows

    gV(v)=1πγγ2+v2.

    The MAP estimator of u is obtained by maximizing the conditional probability of u with given f. Based on Bayes' rule, we have

    argmaxuP(u|f)=argmaxuP(f|u)P(u)P(f). (2.8)

    Equation (2.8) is equivalent to

    argminulogP(f|u)logP(u) (2.9)
    =argminuΩlogP(f(x)|u(x))dxlogP(u), (2.10)

    where the term logP(f(x)|u(x)) presents the degradation process between f and u, and logP(u) denotes the prior information on u. For the Cauchy distribution C(0,γ) and each xΩ, we have

    P(f(x)|u(x))=γπ((u(x)f(x))2+γ2).

    In order to overcome blocking artifacts, we employ the prior P(u)=exp(2λTGV2α(u)). Then we obtain the TGV model for denoising as

    minu BGV2α(Ω){TGV2α(u)+λΩlog(γ2+(uf)2)dx}, (2.11)

    where λ>0 is the regularization parameter.

    Next, we show that problem (2.11) admits at least one solution.

    Theorem 2.1. The problem (2.11) has at least one solution in  BGV2α(Ω), if γ1,λ>0.

    Proof. Clearly, if γ1, there exists a lower bound of the model (2.11). Assume that {uk}kN is a minimizing sequence for problem (2.11).

    By contradiction, we show that {uk} is bounded in L2(Ω) and therefore bounded in L1(Ω). Assume that ||uk||2=+, so there exists a set EΩ and measure(E)0, such that for any xE, uk(x)=+. With fL2(Ω), we have log(γ2+(ukf)2)=+ for all xE, which contradicts to Ωlog(γ2+(uf)2)dx<+.

    Noting that ||u||1 and ||ε(w)||1 are both bounded, we obtain that {uk} is a bounded sequence in  BGV2α(Ω). According to Rellich-Kondrachov compactness theorem, there exists a function uL1(Ω) such that uku. Because TGV2α(u) is proper, semi-continuous and convex in  BGV2α(Ω) [16], we obtain that liminfk+TGV2α(uk)TGV2α(u). Meanwhile, according to the Fatou lemma, we can deduce that

    inf{TGV2α(u)+λΩlog(γ2+(uf)2)dx}   lim infk{TGV2α(uk)+λΩlog(γ2+(ukf)2)dx}TGV2α(u)+λΩlog(γ2+(uf)2)dx,

    which means that u is the minimum point of (2.11), i.e., the problem (2.11) has at least one solution in  BGV2α(Ω). Noting that the model (2.11) is strictly convex, based on the standard arguments in convex analysis[28,29], we obtain that the minimum u is unique.

    In order to obtain a convergent algorithm, we employ the alternating minimization algorithm for the variational model (2.11). The model (2.11) can be discretized as

    minz,u{E(z,u)=TGV2α(z)+λ(Ni=1log(γ2+(uif)2)+η2||uz||22)}. (3.1)

    Remark 3.1. The proximal operator [30] proxf:RnRn of f is defined as

    proxf(v)=argminx(f(x)+12||xv||22).

    The definition indicates that proxf(v) is the trade-off between minimizing f and being near to v. Based on this idea, we convexify the model (2.11) by adding a proximal term. The advantages are as follows:

    (1) A strictly convex model is obtained due to the proximal term.

    (2) The result of each iteration is near to the previous one.

    In order to simplify the alternating minimization algorithm, we first introduce the following notations and definitions:

    S(uk1) (3.2)
    (3.3)

    Now, we solve z-subproblem by (3.2). Based on [31], can be represented by

    (3.4)

    Equation (3.4) will make the calculation of the primal dual method very easy. Note that (for more details, refer to [2,32]), where are positive constant parameters. Therefore the min-max problem of (3.2) can be reformulated as

    (3.5)

    where are the dual variables associated with the sets given by

    Similar to [25], the solution of the min-max problem (3.5) can be solved as follows

    (3.6)
    (3.7)
    (3.8)
    (3.9)
    (3.10)

    where the projection can be computed as

    are positive parameters such that , and represent iteration numbers.

    The optimality condition for (3.3) is

    (3.11)

    Based on the proximal-operator idea, we can take such that the result of each iteration is near to the previous one. Multiplying both sides of (3.11) by , one can obtain that (3.11) is equivalent to

    (3.12)

    where

    (3.13)
    (3.14)
    (3.15)
    (3.16)

    In order to solve (3.12), we need the following proposition.

    Proposition 3.2. [33] A generic cubic equation with real coefficients

    (3.17)

    has at least one solution among the real numbers. Let

    (3.18)

    If there exists a unique real solution of (3.17), the discriminant, has to be positive. Furthermore, if , the only real root of (3.17) is given by

    (3.19)

    Since the problem (3.3) is strictly convex with respect to , then there exists a unique real solution for (3.3) and it can be obtained by (3.19). Instead of the method presented above, the subproblem (3.3) can be solved by the Newton method because the objective function in (3.3) is twice continuously differentiable.

    The alternating minimization algorithm for Cauchy noise removal is given in Algorithm 1, where represents the maximum iteration number.

    Algorithm 1. The alternating minimization algorithm for (3.1).
    input .
    Repeat
    step 1: Update . Initialization:,
    when , .
      Repeat for l = 1:Kz
      step 1.1: Update by (3.6),
      step 1.2: Update by (3.7),
      step 1.3: Update by (3.8),
      step 1.4: Update by (3.9),
      step 1.5: Update by (3.10),
    Define the next iterate as ,
    step 2: Update by (3.3),
    Until or , end.
    step 3: Output -An optimal solution of (3.1).

     | Show Table
    DownLoad: CSV

    In the following section, we prove the convergence of the proposed Algorithm 1.

    Definition 4.1. ([34]). An operator is non-expansive, if for , there holds .

    Clearly, the identity map for all is non-expansive. One can easily check that the product and the sum of two non-expansive operators are also non-expansive respectively. For any fixed , the maps and are non-expansive.

    Definition 4.2. ([34]). Given a non-expansive operator , , for some , is said to be -averaged non-expansive.

    Definition 4.3. ([34]). An operator is called firmly non-expansive, if for any , there holds

    Remark 4.4. An operator is firmly non-expansive if and only if it is -averaged non-expansive.

    Lemma 4.5. ([35]). Let be convex and lower semi-continuous, and . Suppose is defined as follows

    Then is -averaged non-expansive.

    Since is convex and lower semi-continuous, based on Lemma 4.5, it is obvious that is -averaged non-expansive. Note that

    Let , we have

    Noting that is convex and by Lemma 4.5, we have that is -averaged non-expansive.

    Lemma 4.6. ([36]) Let and be -averaged and -averaged non-expansive operators respectively.

    By Lemma 4.5, we obtain that is -averaged non-expansive.

    Definition 4.7. ([37]). A function is proper over a set if for at least one and for all .

    Definition 4.8. ([37]). A function is coercive over a set if for every sequence such that , we have .

    The following Lemma 4.9 can be shown easily, and we omit its proof here.

    Lemma 4.9. The functional in (3.1) is coercive.

    Lemma 4.10. ([28]). Let be a closed, proper and coercive function. Then the set of the minimizers of over is nonempty and compact.

    Lemma 4.11. The set of the fixed points of is non-empty.

    Proof. By Lemma 4.9, the objective function is coercive. Based on Lemma 4.10, the set of minimizers of is non-empty. Set is a minimizer of . Therefore we have

    It indicates that

    Thus we have .

    According to the Krasnoselskii-Mann (KM) theorem [38], noting that is non-expansive and the set of the fixed points of is nonempty, one has that the sequence converges weakly to a fixed point of , for any initial point . Since is strictly convex and differentiable with , one has that the minimizer of is unique. Clearly, the fixed points of are just the minimizers of . Thus the sequence converges to the unique minimizer of . Therefore, we have the following theorem.

    Theorem 4.12. The sequence converges to the unique minimizer of as , for any initial point .

    In this section we provide numerical results to show the performance of the proposed method for image restoration problems under Cauchy noise. Here, we compare our method with existing models as follows: ROF model[1], the median filter[39], SDZ model[13] and MDH model[15]. For ROF model, we use the primal dual method proposed in [31]. For SDZ model and MDH model, we use the source codes of [13] and [15] respectively.

    Considering the quality of the restoration results, we measure them by different evaluation metrics: The peak-signal-to-noise ratio (PSNR) value and the structural similarity (SSIM) value, which are defined as

    where denotes the original signal with mean and is the denoised signal, is the image size,

    where denote, respectively, mean, variance, co-variance of the image and , and are small positive constants. To compare with different approaches easily, we use the same stopping criterion for all the algorithms, that is

    or the maximum number of iterations.

    Combined with relevant reports[13,15,16,17,25,26], we adjust each parameter one by one. For each image in Figure 1, we try our best to tune the parameters of the compared algorithms to obtain the highest PSNR and SSIM. Based on hundreds of experiments, we observe that is the key parameter to control the restoration quality and convergence speed. For the proposed model, ROF model, MDH model and the median filter, the grey level range is [0,255]. For SDZ model, the grey level range is normalized to [0, 1]. For the proposed model, the range of is [0.3, 0.7], , the range of is [15,30], and the range of is [0.9, 3]. For MDH model, the range of is [25,50]. For SDZ model, the range of is [2,9] and the range of is [0.3, 3]. For ROF model, the range of is [1,8].

    Figure 1.  Original images.

    According to the images in Figures 24, images visual quality of our method is better than others. Compared with TV-based methods, block-effects can be more significantly reduced by our method. The reason is that the solution of kernel space of second-order is first-order polynomial, but not the piecewise constant function in space. In Tables 2 and 3, we list PSNR, SSIM values of numerical results. Clearly, PSNR, SSIM values of our method are better than others. According to the zoomed images in Figures 24, our method enhances the image quality and reduces noise more significantly while there is much more noise residual in images of other methods. According to the images in Figure 4, the structure around the eye is better preserved in the proposed method than others.

    Figure 2.  The noisy image, restored images and the locally zoomed images, respectively. For ROF method, . For SDZ method, . For MDH method, . For the proposed method, .
    Figure 3.  The noisy image, restored images and the locally zoomed images, respectively. For ROF method, . For SDZ method, . For MDH method, . For the proposed method, .
    Figure 4.  The noisy image, restored images and the locally zoomed images, respectively. For ROF method, . For SDZ method, . For MDH method, . For the proposed method, .
    Table 2.  PSNR measures for different methods, .
    Image Noisy ROF Median SDZ MDH Ours
    Lena 18.31 26.52 27.91 28.38 30.26 30.81
    Boat 18.01 24.62 26.03 27.12 28.07 29.44
    Montage 19.14 25.88 27.52 28.06 29.88 30.25
    Bridge 19.18 22.17 22.63 24.32 25.25 26.12
    House 17.94 24.56 24.84 25.69 26.71 27.48
    Vehicle 19.20 28.54 28.05 30.98 30.68 31.14
    Saturn 19.04 32.24 34.15 35.65 35.42 36.49
    Parrot 19.08 24.02 27.20 27.19 29.06 29.28

     | Show Table
    DownLoad: CSV
    Table 3.  SSIM measures for deferent methods, .
    Image Noisy ROF Median SDZ MDH Ours
    Lena 0.5377 0.8187 0.8766 0.9061 0.9126
    Boat 0.3252 0.4659 0.7782 0.8276 0.8545
    Montage 0.3230 0.8671 0.8772 0.9210 0.9152
    Bridge 0.4354 0.7354 0.6325 0.7857 0.8112
    House 0.2356 0.7332 0.7510 0.7786 0.8326
    Vehicle 0.5707 0.8129 0.9012 0.9121 0.9236
    Saturn 0.2080 0.8376 0.8636 0.9063 0.9041
    Parrot 0.3999 0.7736 0.8353 0.8471 0.8729

     | Show Table
    DownLoad: CSV

    Based on the Moreau envelop[30] idea and TGV regularization, we propose a new approach to Cauchy noise removal. We show that the solution of the proposed model is unique. In order to solve the new model, an alternating minimization method is employed and its convergence is proved. Numerical results demonstrate that the images quality of our method is better than that of some earlier restoration methods.

    We really appreciate authors of [13] and [15] for their codes.

    We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.



    Conflict of interest



    The authors declare no conflict of interest.

    Author contributions



    I.Z.K. conceived and designed the study, analyzed the data, drafted and finalized the manuscript. R.A.B. contributed to manuscript preparation of the section the importance and sustainable applications of the Paenibacillus polymyxa. All authors read and approved the final manuscript.

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