Over decades, sulfur has been employed for treatment of many dermatological diseases, several skin and soft tissue, and Staphylococcus infections. Because of its abuse, resistant bacterial strains have emerged. Nanotechnology has presented a new horizon to overcome abundant problems including drug resistance. Nano-sized sulfur has proven to retain bactericidal activity. Consequently, the specific aims of this study are exclusively directed to produce various sulfur nanoparticles formulations with control of particle size and morphology and investigate the antibacterial activity response specifically classified by the category of responses of different formulations, for the treatment of acne vulgaris resistant to conventional antibiotics. In this study, we produced uncoated sulfur nanoparticles (SNPs), sulfur nano-composite with chitosan (CS-SNPs), and sulfur nanoparticles coated with polyethylene glycol (PEG-SNPs) and evaluate their bactericidal impact against Staphylococcus aureus and Staphylococcus epidermidis isolated from 173 patients clinically diagnosed acne vulgaris. Accompanied with molecular investigations of ermB and mecA resistance genes distribution among the isolates. Sulfur nanoparticles were synthesized using acid precipitation method and were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), energy dispersed x-ray spectroscopy (EDX), and Fourier transform infrared spectroscopy (FTIR). Moreover, agar diffusion and broth micro-dilution methods were applied to determine their antibacterial activity and their minimum inhibitory concentration. PCR analysis for virulence factors detection. Results: TEM analysis showed particle size of SNPs (11.7 nm), PEG-SNPs (27 nm) and CS-SNPs (33 nm). Significant antibacterial activity from nanoparticles formulations in 100% dimethyl sulfoxide (DMSO) with inhibition zone 30 mm and MIC at 5.5 µg/mL. Furthermore, the prevalence of mecA gene was the most abundant among the isolates while ermB gene was infrequent. Conclusions: sulfur nanoparticles preparations are an effective treatment for most Staphylococcus bacteria causing acne vulgaris harboring multi-drug resistance virulence factors.
Citation: Noha M. Hashem, Alaa El-Din M.S. Hosny, Ali A. Abdelrahman, Samira Zakeer. Antimicrobial activities encountered by sulfur nanoparticles combating Staphylococcal species harboring sccmecA recovered from acne vulgaris[J]. AIMS Microbiology, 2021, 7(4): 481-498. doi: 10.3934/microbiol.2021029
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Over decades, sulfur has been employed for treatment of many dermatological diseases, several skin and soft tissue, and Staphylococcus infections. Because of its abuse, resistant bacterial strains have emerged. Nanotechnology has presented a new horizon to overcome abundant problems including drug resistance. Nano-sized sulfur has proven to retain bactericidal activity. Consequently, the specific aims of this study are exclusively directed to produce various sulfur nanoparticles formulations with control of particle size and morphology and investigate the antibacterial activity response specifically classified by the category of responses of different formulations, for the treatment of acne vulgaris resistant to conventional antibiotics. In this study, we produced uncoated sulfur nanoparticles (SNPs), sulfur nano-composite with chitosan (CS-SNPs), and sulfur nanoparticles coated with polyethylene glycol (PEG-SNPs) and evaluate their bactericidal impact against Staphylococcus aureus and Staphylococcus epidermidis isolated from 173 patients clinically diagnosed acne vulgaris. Accompanied with molecular investigations of ermB and mecA resistance genes distribution among the isolates. Sulfur nanoparticles were synthesized using acid precipitation method and were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), energy dispersed x-ray spectroscopy (EDX), and Fourier transform infrared spectroscopy (FTIR). Moreover, agar diffusion and broth micro-dilution methods were applied to determine their antibacterial activity and their minimum inhibitory concentration. PCR analysis for virulence factors detection. Results: TEM analysis showed particle size of SNPs (11.7 nm), PEG-SNPs (27 nm) and CS-SNPs (33 nm). Significant antibacterial activity from nanoparticles formulations in 100% dimethyl sulfoxide (DMSO) with inhibition zone 30 mm and MIC at 5.5 µg/mL. Furthermore, the prevalence of mecA gene was the most abundant among the isolates while ermB gene was infrequent. Conclusions: sulfur nanoparticles preparations are an effective treatment for most Staphylococcus bacteria causing acne vulgaris harboring multi-drug resistance virulence factors.
This paper is concerned with the regularity criterion of the 3D Boussinesq equations with the incompressibility condition :
{∂tu+u⋅∇u−Δu+∇π=θe3,∂tθ+u⋅∇θ−Δθ=0,∇⋅u=0,(u,θ)(x,0)=(u0,θ0)(x),x∈R3, | (1.1) |
where u=u(x,t) and θ=θ(x,t) denote the unknown velocity vector field and the scalar function temperature, while u0, θ0 with ∇⋅u0=0 in the sense of distribution are given initial data. e3=(0,0,1)T. π=π(x,t) the pressure of fluid at the point (x,t)∈R3×(0,∞). The Boussinesq equation is one of important subjects for researches in nonlinear sciences [14]. There are a huge literatures on the incompressible Boussinesq equations such as [1,2,3,4,6,8,9,10,17,19,20,21,22] and the references therein.
When θ=0, (1.1) reduces to the well-known incompressible Navier-Stokes equations and many results are available. Besides their physical applications, the Navier-Stokes equations are also mathematically significant. From that time on, much effort has been devoted to establish the global existence and uniqueness of smooth solutions to the Navier-Stokes equations.
However, similar to the classic Navier-Stokes equations, the question of global regularity of the weak solutions of the 3D Boussinesq equations still remains a big open problem and the system (1.1) has received many studies. Based on some analysis technique, some regularity criteria via the velocity of weak solutions in the Lebesgue spaces, multiplier spaces and Besov spaces have been obtained in [5,17,19,20,22,23].
More recently, the authors of the present paper [7] showed that the weak solution becomes regular if
∫T0‖u(⋅,t)‖21−r.B−r∞,∞+‖θ(⋅,t)‖21−r.B−r∞,∞1+log(e+‖u(⋅,t)‖Hs+‖θ(⋅,t)‖Hs)dt<∞ for some 0≤r<1 and s≥12, | (1.2) |
where .B−r∞,∞ denotes the homogeneous Besov space. Definitions and basic properties of the Sobolev spaces and the Besov spaces can be find in [18]. For concision, we omit them here.
The purpose of this paper is to improve the regularity criterion (1.2) in the following form.
Theorem 1.1. Let (u,θ) be a smooth solution to (1.1) in [0,T) with the initial data (u0,θ0)∈H3(R3)×H3(R3) with divu0=0 in R3. Suppose that the solution (u,θ) satisfies
∫T0‖u(⋅,t)‖21−r.B−r∞,∞log(e+‖u(⋅,t)‖.B−r∞,∞)dt<∞ for some r with 0≤r<1. | (1.3) |
Then it holds
sup0≤t≤T(‖u(⋅,t)‖2H3+‖θ(⋅,t)‖2H3)<∞. |
That is, the solution (u,θ) can be smoothly extended after time t=T. In other word, if T∗ is the maximal time existence of the solution, then
∫T∗0‖u(⋅,t)‖21−r.B−r∞,∞log(e+‖u(⋅,t)‖.B−r∞,∞)dt<∞. |
Then the solution can be smoothly extended after t=T.
Remark 1.1. The condition (1.3) can be regarded as a logarithmically improved version of the assumption
∫T0‖u(⋅,t)‖21−r.B−r∞,∞dt<∞ for some r with 0≤r<1. |
For the case r=1, we have the following result.
Theorem 1.2. Let (u,θ) be a smooth solution to (1.1) in [0,T) with the initial data (u0,θ0)∈H3(R3)×H3(R3) with divu0=0 in R3. Suppose that there exists a small positive constant η such that
‖u(⋅,t)‖L∞(0,T;.B−1∞,∞(R3))≤η, | (1.4) |
then solution (u,θ) can be smoothly extended after time t=T.
Remark 1.2. Theorem 1.2 can be regarded as improvements and limiting cases of those in [7]. It is worth to point out all conditions are valid for the usual Navier-Stokes equations. We refer to a recent work [7] and references therein.
Remark 1.3. For the case r=0, see [23].
In this section, we will prove Theorem 1.1 by the standard energy method.
Let T>0 be a given fixed time. The existence and uniqueness of local smooth solutions can be obtained as in the case of the Navier-Stokes equations. Hence, for all T>0 we assume that (u,θ) is a smooth solution to (1.1) on [0,T) and we will establish a priori bounds that will allow us to extend (u,θ) beyond time T under the condition (1.3).
Owing to (1.3) holds, one can deduce that for any small ϵ>0, there exists T0=T0(ϵ)<T such that
∫TT0‖u(⋅,t)‖21−r.B−r∞,∞log(e+‖u(⋅,t)‖.B−r∞,∞) dt≤ϵ<<1. | (2.1) |
Thanks to the divergence-free condition ∇⋅u=0, from (1.1)2, we get immediately the global a priori bound for θ in any Lebesgue space
‖θ(⋅,t)‖Lq≤C‖θ0‖Lq for all q∈[2,∞] and all t∈[0,T]. |
Now, multiplying (1.1)2 by θ and using integration by parts, we get
12ddt‖θ‖2L2+‖∇θ‖2L2=0. |
Hence, we obtain
θ∈L∞(0,T;L2(R3))∩L2(0,T;H1(R3)). | (2.2) |
Next, multiplying (1.1)1 by u, we have after integration by part,
12ddt‖u‖2L2+‖∇u‖2L2=∫R3(θe3)⋅udx≤‖θ‖L2‖u‖L2≤C‖u‖L2, |
which yields
u∈L∞(0,T;L2(R3))∩L2(0,T;H1(R3)), | (2.3) |
where we used (2.2) and
∫R3(u⋅∇u)⋅udx=12∫R3(u⋅∇)u2dx=−12∫R3(∇⋅u)u2dx=0 |
by incompressibility of u, that is, ∇⋅u=0.
Now, apply ∇ operator to the equation of (1.1)1 and (1.1)2, then taking the inner product with ∇u and ∇θ, respectively and using integration by parts, we get
12ddt(‖∇u‖2L2+‖∇θ‖2L2)+‖Δu‖2L2+‖Δθ‖2L2=−∫R3∇(u⋅∇)u⋅∇udx+∫R3∇(θe3)⋅∇udx−∫R3∇(u⋅∇)θ⋅∇θdx=I1+I2+I3. | (2.4) |
Employing the Hölder and Young inequalities, we derive the estimation of the first term I1 as
I1=∫R3(u⋅∇)u⋅Δudx≤‖∇⋅(u⊗u)‖L2‖Δu‖L2≤C‖u‖.B−r∞,∞‖∇u‖⋅Hr‖Δu‖L2≤C‖u‖.B−r∞,∞‖∇u‖1−rL2‖Δu‖1+rL2≤12‖Δu‖2L2+C‖u‖21−r.B−r∞,∞‖∇u‖2L2≤12‖Δu‖2L2+C‖u‖21−r.B−r∞,∞(‖∇u‖2L2+‖∇θ‖2L2), |
where we have used the inequality due to [16] :
‖u⊗u‖⋅H1≤C‖u‖.B−r∞,∞‖∇u‖⋅Hr |
and the interpolation inequality
‖w‖.Hs=‖|ξ|sˆw‖L2≤‖w‖1−sL2‖∇w‖sL2 for all 0≤s≤1. |
The term I3 can be estimated as
I3≤C‖∇u‖L2‖∇θ‖2L4≤C‖∇u‖L2‖∇θ‖.B−1∞,∞‖Δθ‖L2≤C‖∇u‖L2‖θ‖.B0∞,∞‖Δθ‖L2≤12‖Δθ‖2L2+C‖θ‖2L∞‖∇u‖2L2≤12‖Δθ‖2L2+C‖θ‖2L∞(‖∇u‖2L2+‖∇θ‖2L2), |
where we have used
‖∇θ‖.B−1∞,∞≤C‖θ‖.B0∞,∞≤C‖θ‖L∞. |
The term I2 can be estimated as
I2≤‖∇u‖L2‖∇θ‖L2≤12(‖∇u‖2L2+‖∇θ‖2L2). |
Plugging all the estimates into (2.4) yields that
ddt(‖∇u‖2L2+‖∇θ‖2L2)+‖Δu‖2L2+‖Δθ‖2L2≤C(12+‖u‖21−r.B−r∞,∞+‖θ‖2L∞)(‖∇u‖2L2+‖∇θ‖2L2). |
Hence, we obtain
ddt(‖∇u(⋅,t)‖2L2+‖∇θ(⋅,t)‖2L2)+‖Δu‖2L2+‖Δθ‖2L2≤C[12+‖u‖21−r.B−r∞,∞+‖θ‖2L∞log(e+‖u‖.B−r∞,∞)](‖∇u‖2L2+‖∇θ‖2L2)log(e+‖u‖.B−r∞,∞)≤C[12+‖u‖21−r.B−r∞,∞+‖θ‖2L∞log(e+‖u‖.B−r∞,∞)](‖∇u‖2L2+‖∇θ‖2L2)log(e+‖u‖H3+‖θ‖H3)≤C[12+‖u‖21−r.B−r∞,∞+‖θ‖2L∞log(e+‖u‖.B−r∞,∞)](‖∇u‖2L2+‖∇θ‖2L2)log(e+κ(t)) |
where κ(t) is defined by
κ(t)=supT0≤τ≤t(‖u(⋅,τ)‖H3+‖θ(⋅,τ)‖H3)forallT0<t<T. |
It should be noted that the function κ(t) is nondecreasing. Moreover, we have used the following fact :
‖u‖.B−r∞,∞≤C‖u‖H3. |
Integrating the above inequality over [T0,t] and applying Gronwall's inequality, we have
‖∇u(⋅,t)‖2L2+‖∇θ(⋅,t)‖2L2+∫tT∗‖Δu(⋅,τ)‖2L2+‖Δθ(⋅,τ)‖2L2dτ≤(‖∇u(⋅,T0)‖2L2+‖∇θ(⋅,T0)‖2L2)×exp(C∫tT0‖u‖21−r.B−r∞,∞log(e+‖u(⋅,τ)‖.B−r∞,∞)log(e+κ(τ))dτ)≤(‖∇u(⋅,T0)‖2L2+‖∇θ(⋅,T0)‖2L2)×exp(Clog(e+κ(t))∫tT0‖u‖21−r.B−r∞,∞log(e+‖u(⋅,τ)‖.B−r∞,∞)dτ)≤˜Cexp(Cϵlog(e+κ(t)))=˜C(e+κ(t))Cϵ | (2.5) |
where ˜C is a positive constant depending on ‖∇u(⋅,T0)‖2L2, ‖∇θ(⋅,T0)‖2L2, T0, T and θ0.
H3−norm. Next, we start to obtain the H3−estimates under the above estimate (2.5). Applying Λ3=(−Δ)32 to (1.1)1, then taking L2 inner product of the resulting equation with Λ3u, and using integration by parts, we obtain
12ddt‖Λ3u(⋅,t)‖2L2+‖Λ4u(⋅,t)‖2L2=−∫R3Λ3(u⋅∇u)⋅Λ3udx+∫R3Λ3(θe3)⋅Λ3udx | (2.6) |
Similarly, applying Λ3=(−Δ)32 to (1.1)2, then taking L2 inner product of the resulting equation with Λ3θ, and using integration by parts, we obtain
12ddt‖Λ3θ(⋅,t)‖2L2+‖Λ4θ(⋅,t)‖2L2=−∫R3Λ3(u⋅∇θ)⋅Λ3θdx, | (2.7) |
Using ∇⋅u=0, we deduce that
12ddt(‖Λ3u(⋅,t)‖2L2+‖Λ3θ(⋅,t)‖2L2)+‖Λ4u(⋅,t)‖2L2+‖Λ4θ(⋅,t)‖2L2=−∫R3[Λ3(u⋅∇u)−u⋅Λ3∇u]⋅Λ3udx+∫R3Λ3(θe3)⋅Λ3udx−∫3R3[Λ3(u⋅∇θ)−u⋅Λ3∇θ]⋅Λ3θdx=Π1+Π2+Π3. | (2.8) |
To bound Π1, we recall the following commutator estimate due to [12]:
‖Λα(fg)−fΛαg‖Lp≤C(‖Λα−1g‖Lq1‖∇f‖Lp1+‖Λαf‖Lp2‖g‖Lq2), | (2.9) |
for α>1, and 1p=1p1+1q1=1p2+1q2. Hence Π1 can be estimated as
Π1≤C‖∇u‖L3‖Λ3u‖2L3≤C‖∇u‖34L2‖Λ3u‖14L2‖∇u‖13L2‖Λ4u‖53L2≤16‖Λ4u‖2L2+C‖∇u‖132L2‖Λ3u‖32L2, | (2.10) |
where we used (2.9) with α=3,p=32, p1=q1=p2=q2=3, and the following Gagliardo-Nirenberg inequalities
{‖∇u‖L3≤C‖∇u‖34L2‖Λ3u‖14L2,‖Λ3u‖L3≤C‖∇u‖16L2‖Λ4u‖56L2. | (2.11) |
If we use the existing estimate (2.1) for T0≤t<T, (2.10) reduces to
Π1≤12‖Λ4u‖2L2+˜C(e+κ(t))32+132Cϵ. | (2.12) |
Using (2.11) again, we get
Π3≤C(‖∇u‖L3‖Λ3θ‖L3+‖∇θ‖L3‖Λ3u‖L3)‖Λ3θ‖L3≤C(‖∇u‖L3+‖∇θ‖L3)(‖Λ3u‖2L3+‖Λ3θ‖2L3)≤16(‖Λ4u‖2L2+‖Λ4θ‖2L2)+˜C(e+κ(t))32+132Cϵ. |
For Π2, we have
Π2≤12(‖Λ3u‖2L2+‖Λ3θ‖2L2)≤˜C(e+κ(t))2. |
Inserting all the inequalities into (2.8) and absorbing the dissipative terms, one finds
ddt(‖Λ3u(⋅,t)‖2L2+‖Λ3θ(⋅,t)‖2L2)≤˜C(e+κ(t))32+132Cϵ+˜C(e+κ(t))2, | (2.13) |
with together with the basic energy (2.2)-([2.3]) yields
ddt(‖u(⋅,t)‖2H3+‖θ(⋅,t)‖2H3)≤˜C(e+κ(t))32+132Cϵ+˜C(e+κ(t))2, | (2.14) |
Choosing ϵ sufficiently small provided that 132Cϵ<12 and applying the Gronwall inequality to (2.14), we derive that
supT0≤τ≤t(‖u(⋅,τ)‖2H3+‖θ(⋅,τ)‖2H3)≤˜C<∞, | (2.15) |
where ˜C depends on ‖∇u(⋅,T0)‖2L2 and ‖∇θ(⋅,T0)‖2L2.
Noting that the right-hand side of (2.15) is independent of t for , we know that (u(⋅,T),θ(⋅,T))∈H3(R3)×H3(R3). Consequently, (u,θ) can be extended smoothly beyond t=T. This completes the proof of Theorem 1.1.
In order to prove Theorem 1.2, we first recall the following local existence theorem of the three-dimensional Boussinesq equations.
Lemma 3.1. Suppose (u,θ)∈Lα(R3), for some α≥3 and ∇⋅u=0. Then, there exists T0>0 and a unique solution of (1.1) on [0,T0) such that
(u,θ)∈BC([0,T0);Lα(R3))∩Ls([0,T0);Lr(R3)),t1su∈BC([0,T0);Lα(R3)) | (3.1) |
Moreover, let (0,T∗) be the maximal interval such that (u,θ) solves (1.1) in C((0,T∗);Lα(R3)), α>3. Then for any t∈(0,T∗)
‖u(⋅,t)‖Lα≥C(T∗−t)α−32α and ‖θ(⋅,t)‖Lα≥C(T∗−t)α−32α, |
with the constant C independent of T∗ and α.
Let (u,θ) be a strong solution satisfying
(u,θ)∈Lα((0,T);Lβ(R3)) for 2α+3β=1 and β>3. |
Then (u,θ) belongs to C∞(R3×(0,T)).
Proof. For all T>0, we assume that (u,θ) is a smooth solution to (1.1) on [0,T) and we will establish a priori bounds that will allow us to extend (u,θ) beyond time T under the condition (1.4).
Similar to the proof of Theorem 1.1, we can show that
(u,θ)∈L∞(0,T;L2(R3))∩L2(0,T;H1(R3)). | (3.2) |
The proof of Theorem 1.2 is divided into steps.
Step Ⅰ. H1−estimation. In order to get the H1−estimates, we apply ∇ operator to the equation of (1.1)1 and (1.1)2, multiply by ∇u and ∇θ, respectively to obtain
12ddt(‖∇u(⋅,t)‖2L2+‖∇θ(⋅,t)‖2L2)+‖Δu(⋅,t)‖2L2+‖Δθ(⋅,t)‖2L2=−∫R3∇(u⋅∇)u⋅∇udx+∫R3∇(θe3)⋅∇udx−∫R3∇(u⋅∇)θ⋅∇θdx=I1+I2+I3. | (3.3) |
Next we estimate I1,I2 and I3 in another way. Hence,
I1≤‖∇u‖3L3≤C‖∇u‖.B−2∞,∞‖Δu‖2L2≤C‖u‖.B−1∞,∞‖Δu‖2L2, |
where we have used the following interpolation inequality due to [16] :
‖w‖L3≤C‖∇w‖23L2‖w‖13.B−2∞,∞. |
By means of the Hölder and Young inequalities, the term I3 can be estimated as
I3≤C‖∇u‖L2‖∇θ‖2L4≤C‖∇u‖L2‖∇θ‖.B−1∞,∞‖Δθ‖L2≤C‖θ‖2.B0∞,∞‖Δθ‖2L2+C‖∇u‖2L2≤C‖θ‖2L∞‖Δθ‖2L2+C‖∇u‖2L2, |
where we have used the following interpolation inequality due to [16] :
‖∇θ‖2L4≤C‖∇θ‖.B−1∞,∞‖Δθ‖L2. |
The term I2 can be estimated as
I2≤‖∇u‖L2‖∇θ‖L2≤12(‖∇u‖2L2+‖∇θ‖2L2). |
Plugging all the estimates into (3.3) yields that
12ddt(‖∇u(⋅,t)‖2L2+‖∇θ(⋅,t)‖2L2)+‖Δu(⋅,t)‖2L2+‖Δθ(⋅,t)‖2L2≤C‖u‖.B−1∞,∞‖Δu‖2L2+C‖θ‖2L∞‖Δθ‖2L2+C(‖∇u‖2L2+‖∇θ‖2L2). |
Under the assumption (1.4), we choose η small enough so that
C‖u‖.B−1∞,∞≤12 . |
Hence, we find that
ddt(‖∇u‖2L2+‖∇θ‖2L2)+‖Δu‖2L2+‖Δθ‖2L2≤C(‖∇u‖2L2+‖∇θ‖2L2). |
Integrating in time and applying the Gronwall inequality, we infer that
‖∇u(⋅,t)‖2L2+‖∇θ(⋅,t)‖2L2+∫T0(‖Δu(⋅,τ)‖2L2+‖Δθ(⋅,τ)‖2L2)dτ≤C. | (3.4) |
Step Ⅱ. H2−estimation. Next, we start to obtain the H2−estimates under the above estimate (3.4). Applying Δ to (1.1)1, then taking L2 inner product of the resulting equation with Δu, and using integration by parts, we obtain
12ddt‖Δu(⋅,t)‖2L2+‖Λ3u(⋅,t)‖2L2=−∫R3Δ(u⋅∇u)⋅Δudx+∫R3Δ(θe3)⋅Δudx | (3.5) |
Similarly, applying Δ to (1.1)2, then taking L2 inner product of the resulting equation with Δθ, and using integration by parts, we obtain
12ddt‖Δθ(⋅,t)‖2L2+‖Λ3θ(⋅,t)‖2L2=−∫R3Δ(u⋅∇θ)⋅Δθdx. | (3.6) |
Adding (3.5) and (3.6), we deduce that
12ddt(‖Δu(⋅,t)‖2L2+‖Δθ(⋅,t)‖2L2)+‖Λ3u(⋅,t)‖2L2+‖Λ3θ(⋅,t)‖2L2=−∫R3Δ(u⋅∇u)⋅Δudx+∫R3Δ(θe3)⋅Δudx−∫R3Δ(u⋅∇θ)⋅Δθdx=K1+K2+K3. | (3.7) |
Using Hölder's inequality and Young's inequality, K1 can be estimated as
K1=∫R3Δ(u⊗u)⋅Δ∇udx≤‖Δ(u⊗u)‖L2‖Δ∇u‖L2≤C‖u‖L∞‖Δu‖L2‖Λ3u‖L2≤12‖Λ3u‖2L2+C‖u‖2L∞‖Δu‖2L2. |
Here we have used the bilinear estimates due to Kato-Ponce [12] and Kenig-Ponce-Vega [13]:
‖Λα(fg)‖Lp≤C(‖Λαg‖Lq1‖f‖Lp1+‖Λαf‖Lp2‖g‖Lq2), |
for α>0, and 1p=1p1+1q1=1+1q2.
From the incompressibility condition, Hölder's inequality and Young's inequality, one has
K3=∫R3Δ(uθ)⋅Δ∇θdx≤‖Δ(uθ)‖L2‖Δ∇θ‖L2≤C(‖u‖L∞‖Δθ‖L2+‖θ‖L∞‖Δu‖L2)‖Λ3θ‖L2≤12‖Λ3θ‖2L2+C(‖u‖2L∞+‖θ‖2L∞)(‖Δu‖2L2+‖Δθ‖2L2). |
For K2, we have
K2≤12(‖Δu‖2L2+‖Δθ‖2L2) |
Inserting all the inequalities into (3.7) and absorbing the dissipative terms, one finds
ddt(‖Δu(⋅,t)‖2L2+‖Δθ(⋅,t)‖2L2)+‖Λ3u(⋅,t)‖2L2+‖Λ3θ(⋅,t)‖2L2≤C(‖u‖2L∞+‖θ‖2L∞)(‖Δu‖2L2+‖Δθ‖2L2). | (3.8) |
Using the following interpolation inequality
‖f‖L∞≤C‖f‖14L2‖Δf‖34L2, |
together with the key estimate (3.4) yield that
∫T0(‖u(⋅,τ)‖2L∞+‖θ(⋅,τ)‖2L∞)dτ≤C<∞. |
Applying the Gronwall inequality to (3.8), we derive that
‖Δu(⋅,t)‖2L2+‖Δθ(⋅,t)‖2L2+∫T0(‖Λ3u(⋅,t)‖2L2+‖Λ3θ(⋅,t)‖2L2)dt≤C. | (3.9) |
By estimates (3.4) and (3.9) as well as the following Gagliardo-Nirenberg's inequality
‖f‖L6≤C‖f‖12L2‖Δf‖12L2, |
it is easy to see that
(u,θ)∈L4(0,T;L6(R3)), |
from which and Lemma 3.1 the smoothness of (u,θ) follows immediately. This completes the proof of Theorem 1.2.
Part of the work was carried out while the first author was long term visitor at University of Catania. The hospitality and support of Catania University are graciously acknowledged.
All authors would like to thank Professor Bo-Qing Dong for helpful discussion and constant encouragement. They also would like to thank the anonymous reviewers for their valuable comments and suggestions to improve the quality of the paper.
All authors declare no conflicts of interest in this paper.
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