Loading [MathJax]/jax/output/SVG/jax.js
Research article Special Issues

Antimicrobial activities encountered by sulfur nanoparticles combating Staphylococcal species harboring sccmecA recovered from acne vulgaris

  • Received: 02 October 2021 Accepted: 22 November 2021 Published: 30 November 2021
  • Over decades, sulfur has been employed for treatment of many dermatological diseases, several skin and soft tissue, and Staphylococcus infections. Because of its abuse, resistant bacterial strains have emerged. Nanotechnology has presented a new horizon to overcome abundant problems including drug resistance. Nano-sized sulfur has proven to retain bactericidal activity. Consequently, the specific aims of this study are exclusively directed to produce various sulfur nanoparticles formulations with control of particle size and morphology and investigate the antibacterial activity response specifically classified by the category of responses of different formulations, for the treatment of acne vulgaris resistant to conventional antibiotics. In this study, we produced uncoated sulfur nanoparticles (SNPs), sulfur nano-composite with chitosan (CS-SNPs), and sulfur nanoparticles coated with polyethylene glycol (PEG-SNPs) and evaluate their bactericidal impact against Staphylococcus aureus and Staphylococcus epidermidis isolated from 173 patients clinically diagnosed acne vulgaris. Accompanied with molecular investigations of ermB and mecA resistance genes distribution among the isolates. Sulfur nanoparticles were synthesized using acid precipitation method and were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), energy dispersed x-ray spectroscopy (EDX), and Fourier transform infrared spectroscopy (FTIR). Moreover, agar diffusion and broth micro-dilution methods were applied to determine their antibacterial activity and their minimum inhibitory concentration. PCR analysis for virulence factors detection. Results: TEM analysis showed particle size of SNPs (11.7 nm), PEG-SNPs (27 nm) and CS-SNPs (33 nm). Significant antibacterial activity from nanoparticles formulations in 100% dimethyl sulfoxide (DMSO) with inhibition zone 30 mm and MIC at 5.5 µg/mL. Furthermore, the prevalence of mecA gene was the most abundant among the isolates while ermB gene was infrequent. Conclusions: sulfur nanoparticles preparations are an effective treatment for most Staphylococcus bacteria causing acne vulgaris harboring multi-drug resistance virulence factors.

    Citation: Noha M. Hashem, Alaa El-Din M.S. Hosny, Ali A. Abdelrahman, Samira Zakeer. Antimicrobial activities encountered by sulfur nanoparticles combating Staphylococcal species harboring sccmecA recovered from acne vulgaris[J]. AIMS Microbiology, 2021, 7(4): 481-498. doi: 10.3934/microbiol.2021029

    Related Papers:

    [1] Ahmad Mohammed Alghamdi, Sadek Gala, Maria Alessandra Ragusa . A regularity criterion of weak solutions to the 3D Boussinesq equations. AIMS Mathematics, 2017, 2(3): 451-457. doi: 10.3934/Math.2017.2.451
    [2] Wei Zhang . A priori estimates for the free boundary problem of incompressible inviscid Boussinesq and MHD-Boussinesq equations without heat diffusion. AIMS Mathematics, 2023, 8(3): 6074-6094. doi: 10.3934/math.2023307
    [3] Zhaoyang Shang . Osgood type blow-up criterion for the 3D Boussinesq equations with partial viscosity. AIMS Mathematics, 2018, 3(1): 1-11. doi: 10.3934/Math.2018.1.1
    [4] Sadek Gala, Maria Alessandra Ragusa . A logarithmically improved regularity criterion for the 3D MHD equations in Morrey-Campanato space. AIMS Mathematics, 2017, 2(1): 16-23. doi: 10.3934/Math.2017.1.16
    [5] Xinli Wang, Haiyang Yu, Tianfeng Wu . Global well-posedness and optimal decay rates for the n-D incompressible Boussinesq equations with fractional dissipation and thermal diffusion. AIMS Mathematics, 2024, 9(12): 34863-34885. doi: 10.3934/math.20241660
    [6] Feng Cheng . On the dissipative solutions for the inviscid Boussinesq equations. AIMS Mathematics, 2020, 5(4): 2869-2876. doi: 10.3934/math.2020184
    [7] Ahmad Mohammad Alghamdi, Sadek Gala, Jae-Myoung Kim, Maria Alessandra Ragusa . The anisotropic integrability logarithmic regularity criterion to the 3D micropolar fluid equations. AIMS Mathematics, 2020, 5(1): 359-375. doi: 10.3934/math.2020024
    [8] Xuemin Xue, Xiangtuan Xiong, Yuanxiang Zhang . Two fractional regularization methods for identifying the radiogenic source of the Helium production-diffusion equation. AIMS Mathematics, 2021, 6(10): 11425-11448. doi: 10.3934/math.2021662
    [9] Oussama Melkemi, Mohammed S. Abdo, Wafa Shammakh, Hadeel Z. Alzumi . Yudovich type solution for the two dimensional Euler-Boussinesq system with critical dissipation and general source term. AIMS Mathematics, 2023, 8(8): 18566-18580. doi: 10.3934/math.2023944
    [10] Ailing Ban . Asymptotic behavior of non-autonomous stochastic Boussinesq lattice system. AIMS Mathematics, 2025, 10(1): 839-857. doi: 10.3934/math.2025040
  • Over decades, sulfur has been employed for treatment of many dermatological diseases, several skin and soft tissue, and Staphylococcus infections. Because of its abuse, resistant bacterial strains have emerged. Nanotechnology has presented a new horizon to overcome abundant problems including drug resistance. Nano-sized sulfur has proven to retain bactericidal activity. Consequently, the specific aims of this study are exclusively directed to produce various sulfur nanoparticles formulations with control of particle size and morphology and investigate the antibacterial activity response specifically classified by the category of responses of different formulations, for the treatment of acne vulgaris resistant to conventional antibiotics. In this study, we produced uncoated sulfur nanoparticles (SNPs), sulfur nano-composite with chitosan (CS-SNPs), and sulfur nanoparticles coated with polyethylene glycol (PEG-SNPs) and evaluate their bactericidal impact against Staphylococcus aureus and Staphylococcus epidermidis isolated from 173 patients clinically diagnosed acne vulgaris. Accompanied with molecular investigations of ermB and mecA resistance genes distribution among the isolates. Sulfur nanoparticles were synthesized using acid precipitation method and were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), energy dispersed x-ray spectroscopy (EDX), and Fourier transform infrared spectroscopy (FTIR). Moreover, agar diffusion and broth micro-dilution methods were applied to determine their antibacterial activity and their minimum inhibitory concentration. PCR analysis for virulence factors detection. Results: TEM analysis showed particle size of SNPs (11.7 nm), PEG-SNPs (27 nm) and CS-SNPs (33 nm). Significant antibacterial activity from nanoparticles formulations in 100% dimethyl sulfoxide (DMSO) with inhibition zone 30 mm and MIC at 5.5 µg/mL. Furthermore, the prevalence of mecA gene was the most abundant among the isolates while ermB gene was infrequent. Conclusions: sulfur nanoparticles preparations are an effective treatment for most Staphylococcus bacteria causing acne vulgaris harboring multi-drug resistance virulence factors.



    1. Introduction and main result

    This paper is concerned with the regularity criterion of the 3D Boussinesq equations with the incompressibility condition :

    {tu+uuΔu+π=θe3,tθ+uθΔθ=0,u=0,(u,θ)(x,0)=(u0,θ0)(x),xR3, (1.1)

    where u=u(x,t) and θ=θ(x,t) denote the unknown velocity vector field and the scalar function temperature, while u0, θ0 with u0=0 in the sense of distribution are given initial data. e3=(0,0,1)T. π=π(x,t) the pressure of fluid at the point (x,t)R3×(0,). The Boussinesq equation is one of important subjects for researches in nonlinear sciences [14]. There are a huge literatures on the incompressible Boussinesq equations such as [1,2,3,4,6,8,9,10,17,19,20,21,22] and the references therein.

    When θ=0, (1.1) reduces to the well-known incompressible Navier-Stokes equations and many results are available. Besides their physical applications, the Navier-Stokes equations are also mathematically significant. From that time on, much effort has been devoted to establish the global existence and uniqueness of smooth solutions to the Navier-Stokes equations.

    However, similar to the classic Navier-Stokes equations, the question of global regularity of the weak solutions of the 3D Boussinesq equations still remains a big open problem and the system (1.1) has received many studies. Based on some analysis technique, some regularity criteria via the velocity of weak solutions in the Lebesgue spaces, multiplier spaces and Besov spaces have been obtained in [5,17,19,20,22,23].

    More recently, the authors of the present paper [7] showed that the weak solution becomes regular if

    T0u(,t)21r.Br,+θ(,t)21r.Br,1+log(e+u(,t)Hs+θ(,t)Hs)dt< for some 0r<1 and s12, (1.2)

    where .Br, denotes the homogeneous Besov space. Definitions and basic properties of the Sobolev spaces and the Besov spaces can be find in [18]. For concision, we omit them here.

    The purpose of this paper is to improve the regularity criterion (1.2) in the following form.

    Theorem 1.1. Let (u,θ) be a smooth solution to (1.1) in [0,T) with the initial data (u0,θ0)H3(R3)×H3(R3) with divu0=0 in R3. Suppose that the solution (u,θ) satisfies

    T0u(,t)21r.Br,log(e+u(,t).Br,)dt< for some r with 0r<1. (1.3)

    Then it holds

    sup0tT(u(,t)2H3+θ(,t)2H3)<.

    That is, the solution (u,θ) can be smoothly extended after time t=T. In other word, if T is the maximal time existence of the solution, then

    T0u(,t)21r.Br,log(e+u(,t).Br,)dt<.

    Then the solution can be smoothly extended after t=T.

    Remark 1.1. The condition (1.3) can be regarded as a logarithmically improved version of the assumption

    T0u(,t)21r.Br,dt< for some r with 0r<1.

    For the case r=1, we have the following result.

    Theorem 1.2. Let (u,θ) be a smooth solution to (1.1) in [0,T) with the initial data (u0,θ0)H3(R3)×H3(R3) with divu0=0 in R3. Suppose that there exists a small positive constant η such that

    u(,t)L(0,T;.B1,(R3))η, (1.4)

    then solution (u,θ) can be smoothly extended after time t=T.

    Remark 1.2. Theorem 1.2 can be regarded as improvements and limiting cases of those in [7]. It is worth to point out all conditions are valid for the usual Navier-Stokes equations. We refer to a recent work [7] and references therein.

    Remark 1.3. For the case r=0, see [23].


    2. Proof of Theorem 1.1

    In this section, we will prove Theorem 1.1 by the standard energy method.

    Let T>0 be a given fixed time. The existence and uniqueness of local smooth solutions can be obtained as in the case of the Navier-Stokes equations. Hence, for all T>0 we assume that (u,θ) is a smooth solution to (1.1) on [0,T) and we will establish a priori bounds that will allow us to extend (u,θ) beyond time T under the condition (1.3).

    Owing to (1.3) holds, one can deduce that for any small ϵ>0, there exists T0=T0(ϵ)<T such that

    TT0u(,t)21r.Br,log(e+u(,t).Br,) dtϵ<<1. (2.1)

    Thanks to the divergence-free condition u=0, from (1.1)2, we get immediately the global a priori bound for θ in any Lebesgue space

    θ(,t)LqCθ0Lq for all q[2,] and all t[0,T].

    Now, multiplying (1.1)2 by θ and using integration by parts, we get

    12ddtθ2L2+θ2L2=0.

    Hence, we obtain

    θL(0,T;L2(R3))L2(0,T;H1(R3)). (2.2)

    Next, multiplying (1.1)1 by u, we have after integration by part,

    12ddtu2L2+u2L2=R3(θe3)udxθL2uL2CuL2,

    which yields

    uL(0,T;L2(R3))L2(0,T;H1(R3)), (2.3)

    where we used (2.2) and

    R3(uu)udx=12R3(u)u2dx=12R3(u)u2dx=0

    by incompressibility of u, that is, u=0.

    Now, apply operator to the equation of (1.1)1 and (1.1)2, then taking the inner product with u and θ, respectively and using integration by parts, we get

    12ddt(u2L2+θ2L2)+Δu2L2+Δθ2L2=R3(u)uudx+R3(θe3)udxR3(u)θθdx=I1+I2+I3. (2.4)

    Employing the Hölder and Young inequalities, we derive the estimation of the first term I1 as

    I1=R3(u)uΔudx(uu)L2ΔuL2Cu.Br,uHrΔuL2Cu.Br,u1rL2Δu1+rL212Δu2L2+Cu21r.Br,u2L212Δu2L2+Cu21r.Br,(u2L2+θ2L2),

    where we have used the inequality due to [16] :

    uuH1Cu.Br,uHr

    and the interpolation inequality

    w.Hs=|ξ|sˆwL2w1sL2wsL2 for all 0s1.

    The term I3 can be estimated as

    I3CuL2θ2L4CuL2θ.B1,ΔθL2CuL2θ.B0,ΔθL212Δθ2L2+Cθ2Lu2L212Δθ2L2+Cθ2L(u2L2+θ2L2),

    where we have used

    θ.B1,Cθ.B0,CθL.

    The term I2 can be estimated as

    I2uL2θL212(u2L2+θ2L2).

    Plugging all the estimates into (2.4) yields that

    ddt(u2L2+θ2L2)+Δu2L2+Δθ2L2C(12+u21r.Br,+θ2L)(u2L2+θ2L2).

    Hence, we obtain

    ddt(u(,t)2L2+θ(,t)2L2)+Δu2L2+Δθ2L2C[12+u21r.Br,+θ2Llog(e+u.Br,)](u2L2+θ2L2)log(e+u.Br,)C[12+u21r.Br,+θ2Llog(e+u.Br,)](u2L2+θ2L2)log(e+uH3+θH3)C[12+u21r.Br,+θ2Llog(e+u.Br,)](u2L2+θ2L2)log(e+κ(t))

    where κ(t) is defined by

    κ(t)=supT0τt(u(,τ)H3+θ(,τ)H3)forallT0<t<T.

    It should be noted that the function κ(t) is nondecreasing. Moreover, we have used the following fact :

    u.Br,CuH3.

    Integrating the above inequality over [T0,t] and applying Gronwall's inequality, we have

    u(,t)2L2+θ(,t)2L2+tTΔu(,τ)2L2+Δθ(,τ)2L2dτ(u(,T0)2L2+θ(,T0)2L2)×exp(CtT0u21r.Br,log(e+u(,τ).Br,)log(e+κ(τ))dτ)(u(,T0)2L2+θ(,T0)2L2)×exp(Clog(e+κ(t))tT0u21r.Br,log(e+u(,τ).Br,)dτ)˜Cexp(Cϵlog(e+κ(t)))=˜C(e+κ(t))Cϵ (2.5)

    where ˜C is a positive constant depending on u(,T0)2L2, θ(,T0)2L2, T0, T and θ0.

    H3norm. Next, we start to obtain the H3estimates under the above estimate (2.5). Applying Λ3=(Δ)32 to (1.1)1, then taking L2 inner product of the resulting equation with Λ3u, and using integration by parts, we obtain

    12ddtΛ3u(,t)2L2+Λ4u(,t)2L2=R3Λ3(uu)Λ3udx+R3Λ3(θe3)Λ3udx (2.6)

    Similarly, applying Λ3=(Δ)32 to (1.1)2, then taking L2 inner product of the resulting equation with Λ3θ, and using integration by parts, we obtain

    12ddtΛ3θ(,t)2L2+Λ4θ(,t)2L2=R3Λ3(uθ)Λ3θdx, (2.7)

    Using u=0, we deduce that

    12ddt(Λ3u(,t)2L2+Λ3θ(,t)2L2)+Λ4u(,t)2L2+Λ4θ(,t)2L2=R3[Λ3(uu)uΛ3u]Λ3udx+R3Λ3(θe3)Λ3udx3R3[Λ3(uθ)uΛ3θ]Λ3θdx=Π1+Π2+Π3. (2.8)

    To bound Π1, we recall the following commutator estimate due to [12]:

    Λα(fg)fΛαgLpC(Λα1gLq1fLp1+ΛαfLp2gLq2), (2.9)

    for α>1, and 1p=1p1+1q1=1p2+1q2. Hence Π1 can be estimated as

    Π1CuL3Λ3u2L3Cu34L2Λ3u14L2u13L2Λ4u53L216Λ4u2L2+Cu132L2Λ3u32L2, (2.10)

    where we used (2.9) with α=3,p=32, p1=q1=p2=q2=3, and the following Gagliardo-Nirenberg inequalities

    {uL3Cu34L2Λ3u14L2,Λ3uL3Cu16L2Λ4u56L2. (2.11)

    If we use the existing estimate (2.1) for T0t<T, (2.10) reduces to

    Π112Λ4u2L2+˜C(e+κ(t))32+132Cϵ. (2.12)

    Using (2.11) again, we get

    Π3C(uL3Λ3θL3+θL3Λ3uL3)Λ3θL3C(uL3+θL3)(Λ3u2L3+Λ3θ2L3)16(Λ4u2L2+Λ4θ2L2)+˜C(e+κ(t))32+132Cϵ.

    For Π2, we have

    Π212(Λ3u2L2+Λ3θ2L2)˜C(e+κ(t))2.

    Inserting all the inequalities into (2.8) and absorbing the dissipative terms, one finds

    ddt(Λ3u(,t)2L2+Λ3θ(,t)2L2)˜C(e+κ(t))32+132Cϵ+˜C(e+κ(t))2, (2.13)

    with together with the basic energy (2.2)-([2.3]) yields

    ddt(u(,t)2H3+θ(,t)2H3)˜C(e+κ(t))32+132Cϵ+˜C(e+κ(t))2, (2.14)

    Choosing ϵ sufficiently small provided that 132Cϵ<12 and applying the Gronwall inequality to (2.14), we derive that

    supT0τt(u(,τ)2H3+θ(,τ)2H3)˜C<, (2.15)

    where ˜C depends on u(,T0)2L2 and θ(,T0)2L2.

    Noting that the right-hand side of (2.15) is independent of t for , we know that (u(,T),θ(,T))H3(R3)×H3(R3). Consequently, (u,θ) can be extended smoothly beyond t=T. This completes the proof of Theorem 1.1.


    3. Proof of Theorem 1.2

    In order to prove Theorem 1.2, we first recall the following local existence theorem of the three-dimensional Boussinesq equations.

    Lemma 3.1. Suppose (u,θ)Lα(R3), for some α3 and u=0. Then, there exists T0>0 and a unique solution of (1.1) on [0,T0) such that

    (u,θ)BC([0,T0);Lα(R3))Ls([0,T0);Lr(R3)),t1suBC([0,T0);Lα(R3)) (3.1)

    Moreover, let (0,T) be the maximal interval such that (u,θ) solves (1.1) in C((0,T);Lα(R3)), α>3. Then for any t(0,T)

    u(,t)LαC(Tt)α32α and θ(,t)LαC(Tt)α32α,

    with the constant C independent of T and α.

    Let (u,θ) be a strong solution satisfying

    (u,θ)Lα((0,T);Lβ(R3)) for 2α+3β=1 and β>3.

    Then (u,θ) belongs to C(R3×(0,T)).

    Proof. For all T>0, we assume that (u,θ) is a smooth solution to (1.1) on [0,T) and we will establish a priori bounds that will allow us to extend (u,θ) beyond time T under the condition (1.4).

    Similar to the proof of Theorem 1.1, we can show that

    (u,θ)L(0,T;L2(R3))L2(0,T;H1(R3)). (3.2)

    The proof of Theorem 1.2 is divided into steps.

    Step Ⅰ. H1estimation. In order to get the H1estimates, we apply operator to the equation of (1.1)1 and (1.1)2, multiply by u and θ, respectively to obtain

    12ddt(u(,t)2L2+θ(,t)2L2)+Δu(,t)2L2+Δθ(,t)2L2=R3(u)uudx+R3(θe3)udxR3(u)θθdx=I1+I2+I3. (3.3)

    Next we estimate I1,I2 and I3 in another way. Hence,

    I1u3L3Cu.B2,Δu2L2Cu.B1,Δu2L2,

    where we have used the following interpolation inequality due to [16] :

    wL3Cw23L2w13.B2,.

    By means of the Hölder and Young inequalities, the term I3 can be estimated as

    I3CuL2θ2L4CuL2θ.B1,ΔθL2Cθ2.B0,Δθ2L2+Cu2L2Cθ2LΔθ2L2+Cu2L2,

    where we have used the following interpolation inequality due to [16] :

    θ2L4Cθ.B1,ΔθL2.

    The term I2 can be estimated as

    I2uL2θL212(u2L2+θ2L2).

    Plugging all the estimates into (3.3) yields that

    12ddt(u(,t)2L2+θ(,t)2L2)+Δu(,t)2L2+Δθ(,t)2L2Cu.B1,Δu2L2+Cθ2LΔθ2L2+C(u2L2+θ2L2).

    Under the assumption (1.4), we choose η small enough so that

    Cu.B1,12 .

    Hence, we find that

    ddt(u2L2+θ2L2)+Δu2L2+Δθ2L2C(u2L2+θ2L2).

    Integrating in time and applying the Gronwall inequality, we infer that

    u(,t)2L2+θ(,t)2L2+T0(Δu(,τ)2L2+Δθ(,τ)2L2)dτC. (3.4)

    Step Ⅱ. H2estimation. Next, we start to obtain the H2estimates under the above estimate (3.4). Applying Δ to (1.1)1, then taking L2 inner product of the resulting equation with Δu, and using integration by parts, we obtain

    12ddtΔu(,t)2L2+Λ3u(,t)2L2=R3Δ(uu)Δudx+R3Δ(θe3)Δudx (3.5)

    Similarly, applying Δ to (1.1)2, then taking L2 inner product of the resulting equation with Δθ, and using integration by parts, we obtain

    12ddtΔθ(,t)2L2+Λ3θ(,t)2L2=R3Δ(uθ)Δθdx. (3.6)

    Adding (3.5) and (3.6), we deduce that

    12ddt(Δu(,t)2L2+Δθ(,t)2L2)+Λ3u(,t)2L2+Λ3θ(,t)2L2=R3Δ(uu)Δudx+R3Δ(θe3)ΔudxR3Δ(uθ)Δθdx=K1+K2+K3. (3.7)

    Using Hölder's inequality and Young's inequality, K1 can be estimated as

    K1=R3Δ(uu)ΔudxΔ(uu)L2ΔuL2CuLΔuL2Λ3uL212Λ3u2L2+Cu2LΔu2L2.

    Here we have used the bilinear estimates due to Kato-Ponce [12] and Kenig-Ponce-Vega [13]:

    Λα(fg)LpC(ΛαgLq1fLp1+ΛαfLp2gLq2),

    for α>0, and 1p=1p1+1q1=1+1q2.

    From the incompressibility condition, Hölder's inequality and Young's inequality, one has

    K3=R3Δ(uθ)ΔθdxΔ(uθ)L2ΔθL2C(uLΔθL2+θLΔuL2)Λ3θL212Λ3θ2L2+C(u2L+θ2L)(Δu2L2+Δθ2L2).

    For K2, we have

    K212(Δu2L2+Δθ2L2)

    Inserting all the inequalities into (3.7) and absorbing the dissipative terms, one finds

    ddt(Δu(,t)2L2+Δθ(,t)2L2)+Λ3u(,t)2L2+Λ3θ(,t)2L2C(u2L+θ2L)(Δu2L2+Δθ2L2). (3.8)

    Using the following interpolation inequality

    fLCf14L2Δf34L2,

    together with the key estimate (3.4) yield that

    T0(u(,τ)2L+θ(,τ)2L)dτC<.

    Applying the Gronwall inequality to (3.8), we derive that

    Δu(,t)2L2+Δθ(,t)2L2+T0(Λ3u(,t)2L2+Λ3θ(,t)2L2)dtC. (3.9)

    By estimates (3.4) and (3.9) as well as the following Gagliardo-Nirenberg's inequality

    fL6Cf12L2Δf12L2,

    it is easy to see that

    (u,θ)L4(0,T;L6(R3)),

    from which and Lemma 3.1 the smoothness of (u,θ) follows immediately. This completes the proof of Theorem 1.2.


    Acknowledgments

    Part of the work was carried out while the first author was long term visitor at University of Catania. The hospitality and support of Catania University are graciously acknowledged.

    All authors would like to thank Professor Bo-Qing Dong for helpful discussion and constant encouragement. They also would like to thank the anonymous reviewers for their valuable comments and suggestions to improve the quality of the paper.


    Conflict of Interest

    All authors declare no conflicts of interest in this paper.




    Declaration of interest



    The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.

    [1] Brook I, Frazier EH, Cox ME, et al. (1995) The aerobic and anaerobic microbiology of pustular acne lesions. Anaerobe 1: 305-307. doi: 10.1006/anae.1995.1031
    [2] Del Rosso JQ, Leyden JJ, Thiboutot D, et al. (2008) Antibiotic use in acne. Cutis 82: 5-12.
    [3] Ito T, Katayama Y, Hiramatsu K (1999) Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315. Antimicrob Agents Chemother 43: 1449-1458. doi: 10.1128/AAC.43.6.1449
    [4] Coutinho V, de LS, Paiva RM, et al. (2010) Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates. Braz J Infect Dis 14: 564-568.
    [5] Farit KH, Urakaev, Botagoz B, et al. (2018) Sulfur nanoparticles stabilized in the presence of water soluble polymer. Mendeleev Commun 28: 161-163. doi: 10.1016/j.mencom.2018.03.017
    [6] Gupta AK, Nicol K (2004) The use of sulfur in dermatology. J Drugs Dermatology 3: 427-431.
    [7] Rai M, Ingle AP, Paralikar P (2016) Sulfur and sulfur nanoparticles as potential antimiocrobial:from traditional medicine to nanomedicine. Expert Rev Anti Infect Ther 14: 969-978. doi: 10.1080/14787210.2016.1221340
    [8] Schneider T, Baldauf A, Ba LA, et al. (2011) Selective antimicrobial activity associated with sulfur nanoparticles. J Biomed Nanotechnol 7: 395-405. doi: 10.1166/jbn.2011.1293
    [9] Choudhury SR, Roy S, Goswami A, et al. (2012) Polyethylene glycol-stabilized sulphur nanoparticles: an effective antimicrobial agent against multidrug-resistant bacteria. J Antimicrob Chemother 67: 1134-1137. doi: 10.1093/jac/dkr591
    [10] Choudhury SR, Mandal A, Ghosh M, et al. (2013) Investigation of antimicrobial physiology of orthorhombic and monoclinic nanoallotropes of sulfur at the interface of transcriptome and metabolome. Appl Microbiol Biotechnol 97: 5965-5978. doi: 10.1007/s00253-013-4789-x
    [11] Choudhury SR, Ghosh M, Goswami A (2012) Inhibitory effects of sulfur nanoparticles on membrane lipids of Aspergillus niger: a novel route of fungistasis. Curr Microbiol 65: 91-97. doi: 10.1007/s00284-012-0130-7
    [12] Mohammed MA, Syeda JTM, Wasan KM, et al. (2017) An overview of chitosan nanoparticles and its application in non-parenteral drug delivery. Pharmaceutics 9: 53. doi: 10.3390/pharmaceutics9040053
    [13] Gutha Y, Pathak JL, Zhang WJ, et al. (2017) Antibacterial and wound healing properties of chitosan/poly(vinyl alcohol)/zinc oxide beads (CS/PVA/ZnO). Int J Biolog Macromol 103: 234-241. doi: 10.1016/j.ijbiomac.2017.05.020
    [14] Qi LF, Xu ZR, Jiang X, et al. (2004) Preparation and antibacterial activity of chitosan nanoparticles. Carbohydrate Research 339: 2693-2700. doi: 10.1016/j.carres.2004.09.007
    [15] Clinical Laboratory Standards Institute CLSI (2019)  Supplement M100, Ed Wayne, PA, USA.
    [16] Shankar S, Pangeni R, Park JW, et al. (2018) Preparation of sulfur nanoparticles and their antibacterial activity and cytotoxic effect. Mater Sci Eng C 92: 508-517. doi: 10.1016/j.msec.2018.07.015
    [17] Kim YH, Kim GH, Yoon KS, et al. (2020) Comparative antibacterial and antifungal activities of sulfur nanoparticles capped with chitosan. Microb Pathog 144: 104178. doi: 10.1016/j.micpath.2020.104178
    [18] Rajeshkumar S, Malarkodi C (2014) In vitro antibacterial activity and mechanism of silver Nanoparticles against foodborne pathogens. Bioinorg Chem Appl 2014: 581890. doi: 10.1155/2014/581890
    [19] Caetano-Anollés (2013) Polymerase Chain Reaction. Brenner's Encyclopedia of Genetics New York: Academic Press, 392-395.
    [20] Ibadin EE, Enabulele IO, Muinah F (2017) Prevalence of mecA gene among staphylococci from clinical samples of a tertiary hospital in Benin City, Nigeria. Afri Health Sci 17: 1000-1010. doi: 10.4314/ahs.v17i4.7
    [21] Wr̨eczycki J, Bielínski DM, Kozanecki M, et al. (2020) Anionic copolymerization of styrene sulfide with elemental sulfur (S8). Materials 13: 2597. doi: 10.3390/ma13112597
    [22] Choudhury SR, Mandal A, Chakravorty D, et al. (2011) Surface-modified sulfur nanoparticles: an effective antifungal agent against Aspergillus niger and Fusarium oxysporum. Appl Microbiol Biotechnol 90: 733-743. doi: 10.1007/s00253-011-3142-5
    [23] Gao ML, Sang RR, Wang G, et al. (2019) Association of pvl gene with incomplete hemolytic phenotype in clinical Staphylococcus aureusInfect Drug Resist 12: 1649-1656. doi: 10.2147/IDR.S197167
    [24] Zhang H, Zheng Y, Gao H, et al. (2016) Identification and Characterization of Staphylococcus aureus Strains with an Incomplete hemolytic phenotype. Front Cell Infect Microbio 6: 146.
    [25] MOON SH, ROH SH, KIM YH, et al. (2012) Antibiotic resistance of microbial strains isolated from Korean acne patients. J Dermatol 39: 833-837. doi: 10.1111/j.1346-8138.2012.01626.x
    [26] Nakase K, Nakaminami H, Takenaka Y, et al. (2014) Relationship between the severity of acne vulgaris and antimicrobial resistance of bacteria isolated from acne lesions in a hospital in Japan. J Med Microbiol 63: 721-728. doi: 10.1099/jmm.0.067611-0
    [27] Doss RW, Abbas Mostafa AM, El-Din Arafa AE, et al. (2016) Relationship between lipase enzyme and antimicrobial susceptibility of Staphylococcus aureus-positive and Staphylococcus epidermidis-positive isolates from acne vulgaris. J Egypt Women's Dermatol Soc 14: 167-172. doi: 10.1097/01.EWX.0000516051.01553.99
    [28] Massalimov IA, Shainurova AR, Khusainov AN, et al. (2012) Production of Sulfur Nanoparticles from Aqueous Solution of Potassium Polysulfi de. Russ J Appl Chem 85: 1832-1837. doi: 10.1134/S1070427212120075
    [29] Deshpande AS, Khomane RB, Vaidya BK, et al. (2008) Sulfur nanoparticles synthesis and characterization from H2S gas, using novel biodegradable iron chelates in W/O microemulsion. Nanoscales Res Lett 3: 221. doi: 10.1007/s11671-008-9140-6
    [30] Suleiman M, Al-Masri M, Ali AA, et al. (2015) Synthesis of nano-sized sulfur nanoparticles and their antibacterial activities. J Mater Environ Sci 2: 513-518.
    [31] Wongwanich S, Tishyadhigama P, Paisomboon S, et al. (2000) Epidemiological analysis of methicillin resistant Staphylococcus aureus in Thailand. Southeast Asian J Trop Med Public Health 31: 72-76.
    [32] Mehndiratta PL, Bhalla P, Ahmed A, et al. (2009) Molecular typing of methicillin-resistant Staphylococcus aureus strains by PCR-RFLP of SPA gene: a reference laboratory perspective. Indian J Med Microbiol 27: 116-122. doi: 10.4103/0255-0857.45363
    [33] Maimona A, Eliman E, Suhair R, et al. (2014) Emergence of vancomycin resistant and methcillin resistant Staphylococus aureus in patients with different clinical manifestations in Khartoum State. J Amer Sci 10: 106-110.
    [34] Cloney L, Marlowe C, Wong A, et al. (1999) Rapid detection of mecA in methicillin resistant Staphylococcus aureus using cycling probe technology. Mol Cell Probes 13: 191-197. doi: 10.1006/mcpr.1999.0235
    [35] Graham JC, Murphy OM, Stewart D, et al. (2000) Comparison of PCR detection of mecA with methicillin and oxacillin disc susceptibility testing in coagulase-negative staphylococci. J Antimicrob Chemother 45: 111-113. doi: 10.1093/jac/45.1.111
    [36] Olayinka BO, Olayinka AT, Obajuluwa AF, et al. (2009) Absence of mecA gene in methicillin-resistant Staphylococcus aureus isolates. Afr J Infect Dis 3: 49-56.
    [37] El-Mahdy TS, Abdalla S, El-Domany R, et al. (2010) Investigation of MLSB and tetracycline resistance in coagulase-negative staphylococci isolated from the skin of Egyptian acne patients and controls. J Am Sci 6: 880-888.
    [38] Gatermann SG, Koschinski T, Friedrich S (2007) Distribution and expression of macrolide resistance genes in coagulase-negative staphylococci. Clin Microbiol Infect 13: 777-781. doi: 10.1111/j.1469-0691.2007.01749.x
    [39] Thakur S, Barua S, Karak N (2015) Self-healable castor oil based tough smart hyper branched polyurethane nanocomposite with antimicrobial attributes. RSC Adv 5: 2167-2176. doi: 10.1039/C4RA11730A
  • This article has been cited by:

    1. Sadek Gala, Maria Alessandra Ragusa, A Regularity Criterion of Weak Solutions to the 3D Boussinesq Equations, 2020, 51, 1678-7544, 513, 10.1007/s00574-019-00162-z
    2. Zhouyu Li, Wenjuan Liu, Qi Zhou, Conditional Regularity for the 3D Damped Boussinesq Equations with Zero Thermal Diffusion, 2024, 55, 1678-7544, 10.1007/s00574-024-00411-w
  • Reader Comments
  • © 2021 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Metrics

Article views(4797) PDF downloads(230) Cited by(18)

Article outline

Figures and Tables

Figures(11)  /  Tables(4)

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return

Catalog