Production, optimization and probiotic characterization of potential lactic acid bacteria producing siderophores

Smita H. Panda; Jyothsna Khanna Goli; Sushrirekha Das; NakulanandaMohanty

doi:10.3934/microbiol.2017.1.88

AIMS Microbiology, 2017, 3(1): 88-107. doi: 10.3934/microbiol.2017.1.88. Research article

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Production, optimization and probiotic characterization of potential lactic acid bacteria producing siderophores

Smita H. Panda, Jyothsna Khanna Goli, Sushrirekha Das, NakulanandaMohanty

1 Department of Zoology, North Orissa University, Baripada, Odisha-757003, India
2 Department of Microbiology, Osmania University, Hyderabad, Andhra Pradesh-500007, India

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The aim of the study was to characterize the probiotic qualities and siderophore production of Enterococcus and Bacillus isolates for possible application for iron nutrition in human and animals strains were selectively isolated from different dairy sources and infant faecal matter. Isolates SB10, JC13 and IFM22 were found to produce maximum siderophore ranging from 65–90% at an optimum pH 7, incubation period of 96 h, agitation speed of 150 rpm and inoculum volume of 15%. SB10 and JC13 were found to show high homology with Enterococcus spp. and IFM22 with Bacillus spp., using partial 16S rRNA sequencing and biochemical characterization. All the three isolates produced hydroxymate type of siderophores under iron stressed conditions and screened for probiotic characters as per WHO guidelines. Strains have shown excellent tolerance to acid, bile salt, sodium chloride and phenol. They were non-haemolytic in nature and exhibited high hydrophobicity and autoaggregation. Our isolates proved to be potent probiotic strains due to their survival under highly acidic conditions and higher tolerance to bile salt. In addition, its colonization efficiency was proved by exhibiting high autoaggregation and hydrophobicity.

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Keywords: probiotic; siderophore; Enterococcus; autoaggregation; hydrophobicity

Citation: Smita H. Panda, Jyothsna Khanna Goli, Sushrirekha Das, NakulanandaMohanty. Production, optimization and probiotic characterization of potential lactic acid bacteria producing siderophores. AIMS Microbiology, 2017, 3(1): 88-107. doi: 10.3934/microbiol.2017.1.88

References:

1. Shanahan F (2004) Probiotics in inflammatory bowel disease—therapeutic rationale and role. Adv Drug Del Rev 56: 809-818.
2. Patel AK, Ahire JJ, Pawar S, et al. (2010) Evaluation of the probiotic characteristics of siderophoregenic Bacillus spp. isolated from dairy waste. App Biochem Biotechnol 160: 140-155.
3. Amagase H (2008) Current marketplace for probiotics: A Japanese perspective. Clin Infec Dis 46: 73-75.
4. Franz CMAP, Melanie HHA, Wilhelm H, Antonio G (2011) Enterococci as probiotics and their implications in food safety. Int J Food Microbiol 151: 125-140.
5. Nichols AW (2007) Probiotics and athletic performance: A systematic review. Curr Sports Med Rep 6: 269-273.
6. Gomes BC, deMelo FBDG, DeMartinis ECP (2010) Dualistic aspects of Enterococcus spp. in foods. Appl Microbial Biotechnol 1119-1125.
7. Hoa NT, Baccigalupi L, HuxhamA, et al. (2000) Characterization of Bacillus species used for oral bacteriotherapy and bacterioprophylaxis of gastrointestinal disorders. Appl Environ Microbiol 66: 5241-5247.
8. Szabo I, Wieler LH, Tedin K, Lydia S, et al. (2009) Influence of a probiotic strain of Enterococcus faecium on Salmonella enteric Serovar Typhimurium DT104 infection in a porcine animal infection model. Appl Environ Microbiol 75: 2621-2628.
9. Duc LH, Hong HA, Barbosa TM, et al. (2004) Characterization of Bacillus probiotics available for human use. Appl Environ Microbiol 70: 2161-2171.
10. Lee JY, Janes BK, Passalacqua KD, et al. (2007) Biosynthetic analysis of the petrobactin siderophore pathway from Bacillus anthracis. J Bacteriol 189: 1698-1710.
11. Patel AK, Deshattiwar MK, Chaudhari BL, et al. (2009a) Production, purification and chemical characterization of the catecholate siderophore from potent probiotic strains of Bacillus spp. Biores Technol 100: 368-373.
12. Ahire JJ, Patil KP, ChaudhariBL, et al. (2011a) A potential probiotic culture ST2 produces siderophore 2,3-dihydroxybenzoylserine under intestinal conditions. Food Chem 127: 387-393.
13. Chincholkar SB, Chaudhari BL, Rane MR (2007) Microbial siderophores in human and plant health care, In: Microbial siderophores, Springer Berlin Heidelberg: 205-214.
14. Sharpe M, Elizabeth-pyer TF (1996) Identification of lactic acid bacteria, In:Gibbs BM & Skinner FA, Identification Methods for Microbiologists, New York: Academic Press, 65-79.
15. Meyer JM, Abdallah MA (1978) The fluorescent pigment of Pseudomonas fluorescens: biosynthesis, purification and physicochemical properties. J General Microbiol 107: 319-328.
16. Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160: 47-56.
17. Payne SM (1994) Detection, isolation and characterization of siderophores. Method Enzymol 235: 329-344.
18. Arnow LE (1937) Colorimetric determination of the components of 3,4 dihydroxyphenyl alanine-tyrosine mixtures. J Biological Chem 118: 531-537.
19. Csaky TZ (1948) On the estimation of bound hydroxylamine in biological materials. Acta Chemica Scandinavica 2: 450-454.
20. Tamura K, Dudley J, Nei M, et al. (2007) MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596-1599.
21. Saitou N, Nei M (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406-425.
22. Tajima F, Nei M (1984) Estimation of evolutionary distance between nucleotide sequences. Mol Biol Evol 1: 269-285.
23. Del Re BS, Miglioli B, Palenzona MD (2000) Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum. Lett Appl Microbiol 31: 438-444.
24. Rosenberg M, Gutnick D, Rosenberg E (1980) Adherence of bacteria to hydrocarbons: A simple method for measuring cell-surface hydrophobicity. FEMS Microbiol Lett 9: 29-33.
25. Holt JG, Krieg RN, Sneath PHA, et al. (1994) Bergey's manual of determinative bacteriology, 9 Eds., Baltimore: Williams and Wilkins.
26. Winkelmann G (2007) Ecology of siderophores with special reference to the fungi. Biometals 20: 379-392.
27. Bendale MS, Chaudhari BL, Chincholkar SB (2010) Influence of environmental factors on siderophore production by Streptomyces fulvissimus ATCC 27431. Pharmainfo Net 16: 36.
28. Lisiecki P, Wysocki P, Mikucki J (1999) Occurrence of siderophores in Enterococci. ZentblBakteriol 289: 807-815.
29. Patel AK, Ahire JJ, Pawar S, Chaudhari BL, et al. (2009b) Comparative accounts of probiotic characteristics of Bacillus spp. isolated from food wastes. Food Res Int 42: 505-510.
30. Sayyed R, Badgujar MD, Sonawane HM, et al. (2005) Production of microbial iron chelators (siderophores) by fluorescent Pseudomonads. Indian J Biotechnol l4: 484-490.
31. Sayyed RZ, Chincholkar SB (2010) Growth and siderophores production in Alcaligenes faecalis is regulated by metal ions. Indian J Microbiol 50: 179-182.
32. Bhakta JN, Ohnishi K, Munekage Y, et al. (2012) Characterization of lactic acid bacteria-based probiotics aspotential heavy metal sorbents. J Appl Microbiol 112: 1193-1206.
33. Ahire JJ, PatilKP, Chaudhari BL, et al. (2011b) Bacillus spp. of human origin: A potential siderophoregenic probiotic bacteria. Appl Biochem Biotechnol 164:386-400.
34. Gangadharan D, Sivaramakrishnan S, Pandey A, et al. (2010) Folate-producing lactic acid bacteria from cow's milk with probiotic characteristics. Int J Dairy Technol 63: 339-348.
35. Kumar P, Ferzin S, Chintan S, et al. (2009) Isolation and characterization of potential probiotic Escherichia coli strains from rat faecal samples. American J Infec Dis 5: 119-124.
36. Pennacchia C, Ercolini D, Blaiotta G, et al. (2004) Selection of Lactobacillus strains fromfermented sausages for their potential use as probiotics. Meat Sci 67: 309-317.
37. Erkkila S, Petaja E (2000) Screening of commercial meat starter cultures at low pH in the presence of bile salts for potential probiotic use. Meat Sci 55: 297-300.
38. Pérez-Miranda S, Cabirol N, George-Téllez R, et al. (2007) O-CAS, a fast and universal method for siderophore detection. J Microbiol Meth 70: 127-131.
39. Aswathy RG, Ismail B, JohnRP, et al. (2008) Evaluation of the probiotic characteristics of newly isolated lactic acid bacteria. App Biochem Biotech 151: 244-255.
40. Qing HU, Dou M, Qi H, et al. (2007) Detection, isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE. J Environ Sci 19: 1114-1119.
41. Gonzalez L, Sandoval H, Sacristan N, et al. (2007) Identification of lactic acid bacteria isolated from Genestoso cheese throughout ripening and study of their antimicrobial activity. Food Cont 18: 716-722
42. Moreno F, Sarantinopoulos MR, Tsakalidou PE, et al. (2006) Therole of application of Enterococci in food and health. Int J Food Microbiol 106: 1-24.
43. Espeche MC, Otero MC, Sesma F, et al. (2009) Screening of surface properties and antagonistic substances production by lactic acid bacteria isolated from the mammary gland of healthy and mastitis cows. Vet Microbiol 135: 346-357.
44. KosB, Suskovic J, Vokavic S, et al. (2003) Adhesion and autoaggregation ability of probiotic strain Lactobacillus acidophilus M92. J Appl Microbiol 94: 981-987.
45. Hamadi F, Latrache H (2008) Comparison of contact angle measurement and microbial adhesion to solvents for assaying electron donor-electron acceptor (acid-base) properties of bacterial surface. Colloid Surface B 65 134-139.

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Copyright Info: 2017, Smita H. Panda, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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