Export file:

Format

  • RIS(for EndNote,Reference Manager,ProCite)
  • BibTex
  • Text

Content

  • Citation Only
  • Citation and Abstract

Biofilm formation in Hafnia alvei HUMV-5920, a human isolate

Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Cantabria, Spain

Topical Section: Microbial biofilms

Hafnia alvei is a Gram-negative, rodshaped, facultative anaerobic bacterium of the family Enterobacteriaceae that has been isolated from various mammals, fish, insects and birds. In humans, case reports of Hafnia-associated enteric infections have been chiefly reported in Spain. Although H. alvei shares some virulence mechanisms with other Gram-negative enteropathogens little is known about the factors that contribute to its pathogenesis or virulence factors and regulatory circuits that may enhance the establishment and survival of H. alvei in the environment. The goal of the present study was to analyze the capacity of a H. alvei clinical isolate (strain HUMV-5920) to form biofilms. Biofilm formation by this strain increases during growth at 28 °C compared to 37 °C. Investigation of multicellular behavior by confocal microscopy, crystal violet and calcofluor staining in this strain showed biofilm formation associated with the production of cellulose. Importantly, several genes related to cellulose production including bcsABZC and yhjQ are present in the H. alvei HUMV-5920 chromosome. The ability of H. alvei to adhere to abiotic surfaces and to form biofilms likely contributes to its persistence in the hospital environment or food processing environments, increasing the probability of causing infections. Therefore, a better understanding of the adherence properties of this species will provide greater insights into the diseases it causes.
  Figure/Table
  Supplementary
  Article Metrics

Keywords biofilm; Hafnia alvei; cellulose

Citation: Itziar Chapartegui-González, María Lázaro-Díez, Santiago Redondo-Salvo, Elena Amaro-Prellezo, Estefanía Esteban-Rodríguez, José Ramos-Vivas. Biofilm formation in Hafnia alvei HUMV-5920, a human isolate. AIMS Microbiology, 2016, 2(4): 412-421. doi: 10.3934/microbiol.2016.4.412

References

  • 1. Ercolini D, Russo F, Ferrocino I, et al. (2009) Molecular identification of mesophilic and psychrotrophic bacteria from raw cow's milk. Food Microbiol 26: 228–231.    
  • 2. Bruhn JB, Christensen AB, Flodgaard LR, et al. (2004) Presence of acylated homoserine lactones (AHLs) and AHL-producing bacteria in meat and potential role of AHL in spoilage of meat. Appl Environ Microb 70: 4293–4302.    
  • 3. Mace S, Cardinal M, Jaffres E, et al. (2012) Evaluation of the spoilage potential of bacteria isolated from spoiled cooked whole tropical shrimp (Penaeus vannamei) stored under modified atmosphere packaging. Food Microbiol 40: 9–17.
  • 4. Rodriguez LA, Vivas J, Gallardo CS, et al. (1999) Identification of Hafnia alvei with the MicroScan WalkAway system. J Clin Microbiol 37: 4186–4188.
  • 5. Klapholz A, Lessnau KD, Huang B, et al. (1994) Hafnia alvei. Respiratory tract isolates in a community hospital over a three-year period and a literature review. Chest 105: 1098–1100.
  • 6. Rodriguez-Guardado A, Boga JA, Diego ID, et al. (2005) Clinical characteristics of nosocomial and community-acquired extraintestinal infections caused by Hafnia alvei. Scand J Infect Dis 37: 870–872.    
  • 7. Laupland KB, Church DL, Ross T, et al. (2006) Population-based laboratory surveillance of Hafnia alvei isolates in a large Canadian health region. Ann Clin Microbiol Antimicrob 5: 683–688.
  • 8. Skurnik D, Nucci A, Ruimy R, et al. (2010) Emergence of carbapenem-resistant Hafnia: the fall of the last soldier. Clin Infect Dis 50: 1429–1431.    
  • 9. Padilla D, Remuzgo-Martinez S, Acosta F, et al. (2013) Hafnia alvei and Hafnia paralvei. Taxonomy defined but still far from virulence and pathogenicity. Vet Microbiol 163: 200–201.
  • 10. Tian B, Moran NA (2016) Genome Sequence of Hafnia alvei bta3_1, a Bacterium with Antimicrobial Properties Isolated from Honey Bee Gut. Genome Announc 4.
  • 11. Viana ES, Campos ME, Ponce AR, et al. (2009) Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk. Biol Res 42: 427–436.
  • 12. Padilla D, Acosta F, Garcia JA, et al. (2009) Temperature influences the expression of fimbriae and flagella in Hafnia alvei strains: an immunofluorescence study. Arch Microbiol 191: 191–198.    
  • 13. Vivas J, Padilla D, Real F, et al. (2008) Influence of environmental conditions on biofilm formation by Hafnia alvei strains. Vet Microbiol 129: 150–155.    
  • 14. Vickery K, Deva A, Jacombs A, et al. (2012) Presence of biofilm containing viable multiresistant organisms despite terminal cleaning on clinical surfaces in an intensive care unit. J Hosp Infect 80: 52–55.    
  • 15. Donlan RM (2001). Biofilms and device-associated infections. Emerg Infect Dis 7: 277–281.
  • 16. Lazaro-Diez M, Redondo-Salvo S, Arboleya-Agudo A, et al. (2016) Whole-Genome Sequence of Hafnia alvei HUMV-5920, a Human Isolate. Genome Announc 4.
  • 17. Romling U, Sierralta WD, Eriksson K, et al. (1998) Multicellular and aggregative behaviour of Salmonella typhimurium strains is controlled by mutations in the agfD promoter. Mol Microbiol 28: 249–264.    
  • 18. Bokranz W, Wang X, Tschape H, et al. (2005) Expression of cellulose and curli fimbriae by Escherichia coli isolated from the gastrointestinal tract. J Med Microbiol 54: 1171–1182.    
  • 19. O’Toole GA, Kolter R (1998) Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol 28: 449–461.    
  • 20. Latasa C, Roux A, Toledo-Arana A, et al. (2005) BapA, a large secreted protein required for biofilm formation and host colonization of Salmonella enterica serovar Enteritidis. Mol Microbiol 58: 1322–1339.    
  • 21. Savini V, Santarelli A, Polilli E, et al. (2013) Hafnia alvei from the farm to the delivery room. Vet Microbiol 163: 202–203.    
  • 22. Beloin C, Roux A, Ghigo JM (2008) Escherichia coli biofilms. Curr Top Microbiol Immunol 322: 249–289.
  • 23. Liu Z, Niu H, Wu S, et al. (2014) CsgD regulatory network in a bacterial trait-altering biofilm formation. Emerg Microbes Infect 3: 159–175.
  • 24. Romling U, Galperin MY (2015) Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions. Trends Microbiol 23: 545–557.    
  • 25. Flemming HC, Wingender J, Szewzyk U, et al. (2016) Biofilms: an emergent form of bacterial life. Nat Rev Microbiol 14: 563–575.    
  • 26. Payne DE, Boles BR (2016) Emerging interactions between matrix components during biofilm development. Curr Genet 62: 137–141.    
  • 27. Zogaj X, Nimtz M, Rohde M, et al. (2001) The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix. Mol Microbiol 39: 1452–1463.    
  • 28. Tan JY, Yin WF, Chan KG (2014) Gene clusters of Hafnia alvei strain FB1 important in survival and pathogenesis: a draft genome perspective. Gut Pathog 6: 29–29.    

 

This article has been cited by

Reader Comments

your name: *   your email: *  

Copyright Info: 2016, José Ramos-Vivas, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

Download full text in PDF

Export Citation

Copyright © AIMS Press All Rights Reserved