AIMS Geosciences, 2016, 2(3): 245-258. doi: 10.3934/geosci.2016.3.245.

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A Novel Method to Quantify Bioavailable Elements and Mobile ATP on Rock Surfaces and Lichens

1 Eawag, Swiss Federal Institute of Aquatic Science and Technology, CH-6047 Kastanienbaum, Switzerland
2 Department of Chemistry, University of Basel, CH-4056 Basel, Switzerland
3 Institute of Biogeochemistry and Pollution Dynamics, ETH Zurich, CH-8092 Zürich, Switzerland
4 Department of Evolutionary Biology and Environmental Studies, University of Zürich, CH-8057 Zurich, Switzerland

The quantification of mobile ions on rock surfaces is essential for the investigation of mineral weathering. A need for such measurements arises from the study of initial soil formation in pioneering environments, the biogeochemical weathering of monuments and buildings, and the chemical reactivity of minerals in general. In the case of mineral surfaces covered by lichens, the quantification of adenosine triphosphate (ATP) is a measure of the vitality of the organisms. To date, non-destructive investigations of rock surfaces and growth of biofilms have generally been limited to visual methods. We evaluated a new technique for the analysis of readily available ions and ATP. For this, a single drop of pure water is spread on bare mineral surfaces or rock-based crustose lichens. The solution is recollected and analyzed for dissolved ions and ATP using a portable capillary electrophoresis instrument and a luminometer, respectively. We illustrate the natural heterogeneity of available ions on freshly broken granite surfaces and the effects of subsequent wetting, freezing, and thawing. In addition, the influence of humidity and age of crustose lichens is demonstrated by ion and ATP analysis.
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Keywords capillary electrophoresis; adenosine triphosphate; rock surface; weathering; granite; lichens; Rhizocarpon geographicum

Citation: Natascha T. Torres, Thomas Steinsberger, Helen Droz-Georget, Beat Müller, Helmut Brandl, Peter C. Hauser, Gerhard Furrer. A Novel Method to Quantify Bioavailable Elements and Mobile ATP on Rock Surfaces and Lichens. AIMS Geosciences, 2016, 2(3): 245-258. doi: 10.3934/geosci.2016.3.245


  • 1. Moses C, Robinson D, Barlow J (2014) Methods for measuring rock surface weathering and erosion: A critical review. Earth-Sci Rev 135: 141-161.    
  • 2. Parker A (1970) An index of weathering for silicate rocks. Geol Mag 107: 501-504.
  • 3. Harnois L (1988) The new CIW index: a new chemical index of weathering. Sediment Geol 55: 319-322.    
  • 4. Matthews JA, Owen G, Winkler S, et al. (2016) A rock-surface microweathering index from Schmidt hammer R-values and its preliminary application to some common rock types in southern Norway. Catena 143: 35-44.    
  • 5. Mohanty B, Gupta A, Das BS (2016) Estimation of weathering indices using spectral reflectance over visible to mid-infrared region. Geoderma 266: 111-119.    
  • 6. Flemming HC, Wingender J (2010) The biofilm matrix. Nat Rev Microbiol 8: 623-633.
  • 7. Paytan A (2012) Mountains, weathering, and climate. Science 335: 810-811.    
  • 8. Zhu B, Wang T, You X, et al. (2008) Nutrient release from weathering of purplish rocks in the Sichuan Basin, China. Pedosphere 18: 257-264.    
  • 9. Yokoyama T, Matsukura Y (2006) Field and laboratory experiments on weathering rates of granodiorite: Separation of chemical and physical processes. Geology 34: 809-812.    
  • 10. Brehm U, Gorbushina A, Mottershead D (2005) The role of microorganisms and biofilms in the breakdown and dissolution of quartz and glass. Palaeogeogr, Palaeoclimatol, Palaeoecol 219: 117-129.    
  • 11. Beschel RE (1961) Dating rock surfaces by lichen growth and its application to the glaciology and physiography (Lichenometry). In: Raasch GO (ed.) Geology of the Arctic. Univ. of Toronto Press, Toronto, 1044-1062.
  • 12. Armstrong R, Bradwell T (2010) Growth of crustose lichens: a review. Geogr Ann 92: 3-17.
  • 13. Conti M, Cecchetti G (2001) Biological monitoring: lichens as bioindicators of air pollution assessment—a review. Environ Pollut 114: 471-492.    
  • 14. Garty J, Kardish N, Hagemeyer J, et al. (1988) Correlations between the concentration of adenosine triphosphate, chlorophyll degradation and the amounts of airborne heavy metals and sulphur in a transplanted lichen. Arch Environ Contam Toxicol 17: 601-611.
  • 15. Kardish N, Ronen R, Bubrick P, et al. (1987) The influence of air pollution on the concentration of ATP and on chlorophyll degradation in the lichen, Ramalina duriaei (De Not.) Bagl. New Phytol 106: 697-706.    
  • 16. Silberstein L, Siegel B, Siegel S, et al. (1996) Comparative studies on Xanthoria parietina, a pollution resistant lichen, and Ramalina duriaei, a sensitive species. I. Effects of air pollution on physiological processes. Lichenologist 28: 355-365.
  • 17. Deer WA, Howie RA, Zussman J (1992) An introduction to the rock forming minerals. 2nd ed., Longman, London.
  • 18. Frey B, Rieder SR, Brunner I (2010) Weathering-associated bacteria from the Damma glacier forefield: physiological capabilities and impact on granite dissolution. Appl Environ Microbiol 76: 4788-4796.    
  • 19. Wongfun N, Götze J, Furrer G, et al. (2013) Effect of water regime and vegetation on initial granite weathering in a glacier forefield: Evidences from CL, SEM, and Nomarski DIC microscopy. Geoderma 211: 116-127.
  • 20. Bernasconi SM, Bauder A, Bourdon B, et al. (2011). Chemical and biological gradients along the Damma glacier soil chronosequence, Switzerland. Vadose Zone J 10: 867-883.    
  • 21. Schaltegger U (1990) The Central Aar granite: highly differentiated calc-alkaline magmatism in the Aar Massif (Central Alps, Switzerland). Eur J Mineral 2: 245-260.    
  • 22. HenssenA, Jahns HM, Santesson J (1974) Lichenes: Eine Einführung in die Flechtenkunde. Thieme, Stuttgart.
  • 23. Proctor M (1983) Sizes and growth-rates of thalli of the lichen Rhizocarpon geographicum on the moraines of the Glacier de Valsorey, Valais, Switzerland. Lichenologist 15: 249-261.    
  • 24. Kubáň P, Reinhardt M, Müller B, et al. (2004) On-site simultaneous determination of anions and cations in drainage water using a flow injection-capillary electrophoresis system with contactless conductivity detection. J Environ Monitoring 6: 169-174.    
  • 25. Kubáň P, Nguyen HTA, Macka M, et al. (2007) New fully portable instrument for the versatile determination of cations and anions by capillary electrophoresis with contactless conductivity detection. Electroanalysis 19: 2059-2065.    
  • 26. Torres NT, Och LM, Hauser PC, et al. (2014) Early diagenetic processes generate iron and manganese oxide layers in the sediments of Lake Baikal, Siberia. Environ Sci: Processes Impacts 16: 879-889.    
  • 27. Torres NT, Hauser PC, Furrer G, et al. (2013) Sediment porewater extraction and analysis combining filter tube samplers and capillary electrophoresis. Environ Sci: Processes Impacts 15: 715-720.    
  • 28. Bisdom E, Stoops G, Delvigne J, et al. (1982) Micromorphology of weathering biotite and its secondary products. Pedologie 32: 225-252.
  • 29. Furrer G, Sollins P, Westall JC (1990) The study of soil chemistry through quasi-steady-state models: II. Acidity of soil solution. Geochim Cosmochim Acta 54: 2363-2374.    
  • 30. Armstrong RA (1983) Growth curve of the lichen Rhizocarpon geographicum. New Phytol 94: 619-622.    
  • 31. Armstrong RA, Smith S (1997) Factors associated with degeneration of the thallus centre in foliose lichens. Symbiosis 22: 293-302.
  • 32. Lee M, Parsons I (1999) Biomechanical and biochemical weathering of lichen-encrusted granite: textural controls on organic–mineral interactions and deposition of silica-rich layers. Chem Geol 161: 385-397.    
  • 33. Silva B, Prieto B (2004) Deteriorative effects of lichens on granite monuments. In: St. Clair L, Seaward M (eds.) Biodeterioration of Stone Surfaces. Springer, Netherlands, 69-77.


This article has been cited by

  • 1. Pavel Kubáň, Peter C. Hauser, Contactless conductivity detection for analytical techniques: Developments from 2016 to 2018, ELECTROPHORESIS, 2018, 10.1002/elps.201800248

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