The mechanism of kaolin clay flocculation by a cation-independent bioflocculant produced by <em>Chryseobacterium daeguense </em>W6

Weijie Liu; Liu Cong; Hongli Yuan; Jinshui Yang

doi:10.3934/environsci.2015.2.169

AIMS Environmental Science, 2015, 2(2): 169-179. doi: 10.3934/environsci.2015.2.169. Research article

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The mechanism of kaolin clay flocculation by a cation-independent bioflocculant produced by Chryseobacterium daeguense W6

Weijie Liu, Liu Cong, Hongli Yuan, Jinshui Yang

1 School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu Province, China;
2 State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China

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In recent years, several novel cation-independent bioflocculants have been reported, which can avoid the secondary contamination caused by addition of cations. However, compared with cation-dependent bioflocculants, the flocculating mechanism of cation-independent bioflocculants is largely unknown. In this study, a cation-independent bioflocculant MBF-W6 produced by Chryseobacterium daeguense W6 was used as a model to investigate the flocculating mechanism. The results showed that the major flocculating component of MBF-W6 is a complex of proteins and polysaccharides. The zeta potential results indicated that kaolin clay particles were not precipitated due to charge neutralization and the bridging mediated by cations did not play a major role in the flocculating process. These results are consistent with the fact that MBF-W6 is a cation-independent bioflocculant. Further scanning electron microscopic observation showed that MBF-W6 induced flocs formed tight packed structure, suggesting that the kaolin clay particles maybe directly attached and bridged by bioflocculant MBF-W6. In addition, we also found out that Fe³⁺ ions inhibit the flocculating activity of MBF-W6 by affecting –COO^- and –NH groups. Therefore this study can improve our understanding on flocculating mechanism of cation-independent bioflocculants.

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Keywords: bioflocculant; cation-independent; flocculating mechanism; polysaccharide; Chryseobacterium daeguense

Citation: Weijie Liu, Liu Cong, Hongli Yuan, Jinshui Yang. The mechanism of kaolin clay flocculation by a cation-independent bioflocculant produced by Chryseobacterium daeguense W6. AIMS Environmental Science, 2015, 2(2): 169-179. doi: 10.3934/environsci.2015.2.169

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Copyright Info: 2015, Weijie Liu, Jinshui Yang, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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