Assessment of heavy metal tolerance and hexavalent chromium reducing potential of <em>Corynebacterium paurometabolum</em> SKPD 1204 isolated from chromite mine seepage

Satarupa Dey; Amal Kanti Paul; Satarupa Dey; Amal Kanti Paul

doi:10.3934/bioeng.2016.3.337

AIMS Bioengineering

2016, Volume 3, Issue 3: 337-351. doi: 10.3934/bioeng.2016.3.337

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Assessment of heavy metal tolerance and hexavalent chromium reducing potential of Corynebacterium paurometabolum SKPD 1204 isolated from chromite mine seepage

Satarupa Dey ,
Amal Kanti Paul ^,

Microbiology Laboratory, Department of Botany, University of Calcutta, Kolkata 700019, India

Received: 14 May 2016 Accepted: 20 July 2016 Published: 27 July 2016

Corynebacterium paurometabolum SKPD 1204 (MTCC 8730), a heavy metal tolerant and chromate reducing bacterium isolated from chromite mine seepage of Odisha, India has been evaluated for chromate reduction under batch culture. The isolate was found to tolerate metals like Co(II), Cu(II), Ni(II), Mn(II), Zn(II), Fe(III) and Hg(II) along with Cr(VI) and was resistant to different antibiotics as evaluated by disc-diffusion method. The isolate, SKPD 1204 was found to reduce 62.5% of 2 mM Cr(VI) in Vogel Bonner broth within 8 days of incubation. Chromate reduction capability of SKPD 1204 decreased with increase in Cr(VI) concentration, but increased with increase in cell density and attained its maximum at 10¹⁰cells/mL. Chromate reducing efficiency of SKPD 1204 was promoted in the presence of glycerol and glucose, while the highest reduction was recorded at pH 7.0 and 35 °C. The reduction process was inhibited by divalent cations Zn(II), Cd(II), Cu(II), and Ni(II), but not by Mn(II). Anions like nitrate, phosphate, sulphate and sulphite was found to be inhibitory to the process of Cr(VI) reduction. Similarly, sodium fluoride, carbonyl cyanide m-chlorophenylhydrazone, sodium azide and N, N,-Di cyclohexyl carboiimide were inhibitory to chromate reduction, while 2,4-dinitrophenol appeared to be neither promotive nor inhibitory to the process.

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Citation: Satarupa Dey, Amal Kanti Paul. Assessment of heavy metal tolerance and hexavalent chromium reducing potential of Corynebacterium paurometabolum SKPD 1204 isolated from chromite mine seepage[J]. AIMS Bioengineering, 2016, 3(3): 337-351. doi: 10.3934/bioeng.2016.3.337

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Abstract

Due to a high surface to volume ratio, significant transport properties, and confinement effects resulting from the nanoscale dimensions, nanostructured materials are extensively studied for energy-related applications such as solar cells, catalysts, thermoelectrics, lithium ion batteries, super-capacitors, and hydrogen storage systems. Therefore extensive studies in this field are necessary to accomplish this challenge for the next decades. The editors of the Special Issue on Nanomaterials for energy and environmental applications in AIMS Materials Science tried to bring together experts in this field to describe the current state of the art of the study and to formulate new research directions. The Special issue contains eleven manuscripts from research groups in different countries presenting relevant results on energy related materials such as transparent conducting nanostructures (TiO₂, SnO₂, ZnO, Ga₂O₃, CuO, In₂O₃, GeO₂, In₂Ge₂O₇, ZnGa₂O₄, Zn₂GeO₄,Sb₂O₃),semiconductors (Si, Ge, CdSe, Ge₃N₄, InN) and organics. The contributors are at the forefront of the discipline, and their insightful scientific presentations will surely affect the next generations of materials scientists. The publications reflect the strong international interactions among physicists, chemists, engineers, materials scientists and technologists working in this field as well as the advantages, limitations and challenges of applications of nanomaterials. We hope that readers will find the papers interesting and stimulating further discussions in the field.

As guest editors of the special issue, we want to thank Dr. Shengjiao Pang, Editor and the technical staff who supported our initiative and made this special issue possible. Also, we thank all contributors of selected papers presented herein.

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