Research article Special Issues

Changes in Spontaneous Working-memory, Memory-recall and Approach-avoidance following “Low Dose” Monosodium Glutamate in Mice

  • The study investigated the effects of ‘low doses’ of monosodium glutamate (MSG) on hippocampal-related (spontaneous working-memory, memory-recall and anxiety) behaviours, and hippocampal glutamate/glutamine levels. A two-trial Y-maze test and 8-arm radial-arm maze spontaneous working-memory test were used to assess the effects of acute and repeated administration of MSG, on novel-arm choice on retrial and spatial working-memory; while anxiety-related behaviors were assessed in the elevated plus maze. In the elevated plus maze, radial-arm maze and Y-maze, MSG administration was associated with significant anxiolytic and memory-enhancing effects at 10 mg/kg (after both acute and repeated dosing); however, higher doses used in this study were associated with significant anxiogenesis and memory retardation. Hippocampal glutamate and glutamine levels did not increase significantly at any of the doses of MSG. In conclusion, MSG administration at low doses was associated with significant changes in hippocampal-dependent behaviours without a concomitant significant shift in hippocampal glutamate/glutamine levels.

    Citation: Olakunle J. Onaolapo, Adejoke Y. Onaolapo, Moses A. Akanmu, Gbola Olayiwola. Changes in Spontaneous Working-memory, Memory-recall and Approach-avoidance following “Low Dose” Monosodium Glutamate in Mice[J]. AIMS Neuroscience, 2016, 3(3): 317-337. doi: 10.3934/Neuroscience.2016.3.317

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  • The study investigated the effects of ‘low doses’ of monosodium glutamate (MSG) on hippocampal-related (spontaneous working-memory, memory-recall and anxiety) behaviours, and hippocampal glutamate/glutamine levels. A two-trial Y-maze test and 8-arm radial-arm maze spontaneous working-memory test were used to assess the effects of acute and repeated administration of MSG, on novel-arm choice on retrial and spatial working-memory; while anxiety-related behaviors were assessed in the elevated plus maze. In the elevated plus maze, radial-arm maze and Y-maze, MSG administration was associated with significant anxiolytic and memory-enhancing effects at 10 mg/kg (after both acute and repeated dosing); however, higher doses used in this study were associated with significant anxiogenesis and memory retardation. Hippocampal glutamate and glutamine levels did not increase significantly at any of the doses of MSG. In conclusion, MSG administration at low doses was associated with significant changes in hippocampal-dependent behaviours without a concomitant significant shift in hippocampal glutamate/glutamine levels.


    1. Introduction

    Volatile Organic Compounds (VOCs) include a large group of compounds which are named according to their boiling points, from very volatile organic compounds (VVOCs) with boiling point around 50 °C to semi-volatile organic compounds (SVOCs) with boiling points of 220-260 °C [1]. VOCs consist of different functional groups such as aliphatic hydrocarbons, aromatic hydrocarbons, halogens etc. In terms of health, some VOCs have severe adverse health effects due to acute or chronic exposure [2]. Among them, benzene is one of the most worrying compounds due to its confirmed human carcinogenicity [2,3]; thus its limit value in air has been regulated by many countries (mostly around <5 µg/m3 annually).

    Sources of VOCs indoors are ubiquitous such as building and decoration products, air fresheners, household cleaning agents, some cooking and heating fuels, many consumer products, office equipment etc. [4,5,6,7]. Indoor VOCs are influenced by climatic factors, such as humidity and temperature, as well as occupants' behaviours, such as smoking indoors, household cleaning activity (both frequency, duration and household cleaning agent preferences etc.), ventilation activity (type of ventilator i.e., natural/mechanical and ventilation amount) [8,9].

    In addition to the direct effect of indoor temperature fluctuations, season also has an indirect effect on observed indoor VOC levels by means of heating or cooling intentions of the occupants. Thus, occupants tend to close indoor environments during wintertime in order to prevent heat loss, while they tend to cool their indoor environments during the summertime by any means of ventilation. Studies have shown that indoor VOC levels vary seasonally similar to outdoor VOC levels, depending on the predominant source at the time of measurements such as type of domestic heating fuel/industrial fuel or photochemical activity [6,7,10,11,12,13].

    There are plenty of studies in the literature on indoor VOC levels, yet few of them focus on their potential sources or factors influencing observed indoor VOCs [14]. Residential VOC levels and their sources are not associated with a single source or a single factor; they are due to a mixture of multiple sources. Thus, all potential sources and factors that may influence indoor VOC levels should be taken into account to obtain a basic indoor air pollution index (IAPi).

    Self-Organizing Maps (SOM) are competitive and unsupervised forms of artificial neural networks (ANNs), pioneered by the Finnish professor Teuvo Kohonen (1981) [15]. One of the most widely used ANN algorithms is SOM, which is used extensively for information extraction without prior knowledge and efficiency of visualization [16]. In this study, SOM is used for overall air pollution classification including all target VOCs.

    The aims of this study are: (i) assessment of long-term indoor VOC exposure levels in six different homes in three different towns in Çanakkale, Turkey; (ii) characterizing the indoor environments by scoring the building/environmental factors and occupants' habits; (iii) estimating the associations between indoor VOC levels and building/environmental factors and occupants' habits to form a IAPi, and (iv) creating an indoor air pollution index for observed VOC levels (IAPvoc) by categorizing the target VOC levels from good towards bad by using basic numerical methods (i.e. SOM, rounded average, and maximum value).


    2. Materials and Methods


    2.1. Study design

    Indoor VOC levels were measured in homes (n = 6) located in three different towns which are Çanakkale city center (urban and marine-road traffic), Çan (semi-urban and industrial), and Lapseki (rural and marine-road traffic) in Çanakkale, Turkey. Sampling points are shown in Figure 1. Three of the homes were located in the city center, one home was located in Lapseki, and two homes were located in Çan. All indoor environments were selected randomly from among volunteer participants of a previous health survey of the Çanakkale HealthAir Study [10]. Indoor VOC samples were collected every month from living rooms of the sampling sites for a year. Also, temperature and relative humidity values were recorded at the time of sampling. All sampling sites were naturally ventilated homes.

    Figure 1. Sampling sites (*denotes the sampling sites 1: Center, 2: Lapseki, and 3: Can).

    2.2. VOCs sampling and analyses

    Monthly indoor VOC samples were collected from the sampling sites using a passive sampling principle throughout one year [17]. VOC samples were collected on Tenax TA-Carbograph1TD dual-bed sorbent tubes and analyzed by Thermal Desorber (Unity-2, Markes Inc., USA) - Gas Chromatography-Flame Ionization Detector (7890A, Agilent Technologies Inc., USA). A capillary column (DB-VRX, 75 m × 0.45 mm × 2.55 mm, Agilent Technologies Inc., USA) was used to detect VOCs. Concentrations of benzene, toluene, xylenes, i.e. BTX, and total volatile organic compounds (TVOC) were assessed. TVOC concentrations were quantified as toluene-equivalent from C6 (n-hexane) to C16 (n-hexadecane) [10,18,19]. A certified standard VOC mixture (Volatile Arom. + Unsat. Org. Comp. Mix 1, Dr. Ehrenstorfer, Germany) was used for five-point calibration. The limit of quantification (LOQ) for individual VOCs was calculated [10,20] to be around 1.0 µg m−3 on average. Quality Assurance/Quality Control (QA/QC) procedures followed in this study were the same as those in Mentese et al. [10].


    2.3. Home inventory

    A detailed questionnaire was given to an occupant of each sampling site, including questions about building and environmental parameters (i.e. floor number, room area, flooring material type, last floor covering/wall painting time, and amount of carpet/wooden product in the room), as well as occupants' habits (i.e. home cleaning frequency, pesticide/naphthalene-air freshener usage, cooking/heating fuel type, and average daily ventilation duration) [21]. The sites were investigated visually to monitor the building/environmental related factors for IAPi scoring [14].


    2.4. Evaluation of the data

    Bivariate regression analyses and other basic computations were applied using MS Excel. Indoor VOC concentrations were analyzed as independent variables; while scores of building/environmental factors, occupants' habits and total factors were dependent variables to find the associations between IAQ parameters and other factors. VOC levels below LOQ were assigned with 1/2 LOQ values to increase the precision of the computations.

    Three different basic numerical methods were used to classify the quality of indoor environments in terms of target compounds of TVOC, benzene, toluene, and xylenes: They are (i) self-organizing map (SOM), (ii) rounded average approach and (iii) maximum value approach.

    Artificial Neural Networks (ANNs), divided into supervised and unsupervised learning, are widely used in environmental models. Supervised learning is generally used for data estimation based on prior knowledge, while unsupervised learning is useful for classification of problems without previous knowledge [22]. In this study, Self-Organizing Map (SOM), an unsupervised form of artificial neural networks, was used for indoor air quality (IAQ) classification. There are three procedures for applying SOM: data normalization, training, and extracting information.

    In the normalization step, we transformed the pollutants within the range of 0-1, since all parameters had equal importance. The formula used for normalization is given in the equation below:

    Ni=XiXminXmaxXmin

    where Xi is the value of pollutant, Xmin, and Xmax are minimum and maximum values of pollutant, respectively. In this study, MATLABR2014a's Neural Clustering toolbox was used for SOM modelling with 10x10 neurons on the output layer (Scheme 1). The training strategy is based on "winner takes all". After the training of SOM, the results can be post-processed based on visualization and clustering [23].Indoor environments were classified into three categories by SOM: (i) good, (ii) moderate, and (iii) bad.

    Scheme 1. Neural network flow chart.

    Besides SOM, two other basic numerical methods were used to classify the indoor environments according to their VOC levels. For the rounded average and the maximum value approaches, self-performed VOC classes tending from good towards bad for each target VOC were used (see Table 1). To set the upper and lower limits of each class for the target VOCs, available guideline values, building certification/rating system limit values, suggested levels for health, and the typical values of the VOCs observed in extensive studies were taken into account as a whole.

    For the rounded average method, exceeding or non-exceeding the upper limits of each class ("good" = 0, "moderate" = 1, and "bad" = 2) was scored for each target compound, given in Table 1. Thus, the total score for an environment for four target pollutants ranged between zero (all of the target compounds are in "good" class) and eight (all of the target compounds are in "bad" class). Final IAPvoc for the rounded average method is computed using total average score of the environment and rounding the IAPvoc, if it is a floating point number. Estimating the IAPvoc class by the maximum value method is quite similar to the rounded average method. The only difference is the maximum score value amongst the four target pollutants' scores for each environment is used to estimate the final IAPvoc class, instead of rounding the average score.

    Table 1. IAPvoc criteria* used for the rounded average and the maximum value methods.
    IAPvoc Concentration (µg/m3)
    TVOC Benzene Toluene Xylenes
    Good < 200 < 2 < 15 < 5
    Moderate ≥ 200-1000 ≥ 2-5 ≥ 15-30 ≥ 5-10
    Bad > 1000 > 5 > 30 > 10
    *coding: "good" = 0, "moderate" = 1, "bad" = 2
     | Show Table
    DownLoad: CSV

    3. Results and Discussion


    3.1. Indoor VOC levels

    The highest levels of TVOC, benzene, toluene, and xylenes occurred in industrial, rural, and urban sites, in descending order. In general, VOC levels were found to be higher throughout the heating season (October-March) compared to other months (Figures 2-5). Exceptionally, summertime VOC levels were higher in Ind-1 sampling site than wintertime levels, probably due to the enhanced reactions due to photochemical activity together with the industrial emissions. TVOC levels were lower than 1000 µg/m3 in urban and rural sampling sites, while it was over 1000 µg/m3 for the industrial ones (see Figure 2).

    Benzene levels exceeded the limit value of 2008/50/EC, 5 µg/m3 [24], only at Ind-1 sampling site during the heating period, while the rest of the time it was below its limit value in all sampling sites (see Figure 3). The lowest benzene levels occurred during the non-heating period in all sampling sites, and in general, the highest benzene levels occurred during the heating period.

    Similar to the trends were observed for TVOC and benzene, levels of toluene and xylenes were found to be higher in the industrial town, particularly Ind-1 sampling site, compared to other towns (see Figures 4-5). Similar to TVOC levels trend occurred in Ind-1, the highest toluene/xylene levels were found during the non-heating period, probably due to more frequent ventilation with outdoors where the enhanced photochemistry of toluene/xylenes might have occurred due to industrial sources.

    Figure 2. Levels (µg/m3) of Indoor TVOC (Urb: urban, Rur: rural, and Ind: industrial).
    Figure 3. Levels (µg/m3) of Indoor Benzene (Urb: urban, Rur: rural, and Ind: industrial).
    Figure 4. Levels (µg/m3) of Indoor Toluene (Urb: urban, Rur: rural, and Ind: industrial).
    Figure 5. Levels (µg/m3) of Indoor Xylenes (Urb: urban, Rur: rural, and Ind: industrial).

    Similar to this study results, toluene was the first ranked compound among BTEX in another study conducted in Ankara, Turkey [6]. Also, BTEX levels were related to the proximity of outdoor BTEX sources such as traffic and gas stations or availability of VOC sources indoors such as activities related to construction/renovation, kitchen, and smoking [7,25,26,27,28].

    As mentioned earlier, among BTX, benzene is a concern due to it carcinogenicity [2,29]. The limit value for annual average benzene concentration (5 µg/m3), set by European Union [24], was only exceeded in one of the sampling sites located in the industrial region only during the heating season not for annual average value. Thus, it can be said that benzene levels can be assumed to be "safe" at the time of sampling, but definitely must be improved particularly in industrial sites. Mentese et al. (2012) also observed the average benzene concentration as > 5 µg/m3 in some sampling sites with smokers and sites that were close to high density traffic sources in wintertime [6]. Smoking and traffic related activities are the major sources of benzene exposure [30,31]. Benzene levels ranged widely in the EXPOLIS study and the highest benzene levels were observed at sampling sites in Greece, Czech Republic and Italy [32].


    3.2. Indoor air pollution index (IAPi)

    Building and environmental factors together with occupants' habits were scored to obtain a basic IAPi for the homes (Table 2). A total of 14 factors were scored between 5 to 25; building/environmental factors were scored between 4 and 16, and the occupants' habits were scored between 1 and 9. The higher score the environment has, the worse the indoor air quality (higher IAPi value). Some questions have yes/no answers (coded as 0 and 1), while some are multiple choice and thus they were classified logically according to their potential to influence the indoor air pollution, e.g. as the home is located on the lowest floors, contribution of VOC emissions from traffic sources assumed is assumed to be higher. Thus, answers with high scores (2 or 3) are expected to influence the IAQ more, when compared to answers with low scores (0 or 1). For some of the questions scores start from 0 (e.g. no observable effect or negligible effect), while some start from 1 (e.g. this factor has a certain degree of effect anyway).

    Table 3 shows the indoor air pollution indexing criteria applied in this study. Accordingly, the environments were classified into three groups: "good", "moderate", and "bad" with the IAPi approach. IAPi is includes two main sub-groups that affect the observed IAQ, which are building/environmental factors and occupants' habits. Also, these two subgroups were evaluated separately in addition to the total score computed for each environment (IAPi value) to find the predominant group with most influence on IAQ. Notice that one of the factors is available or active only during the heating period (i.e. heating fuel type). Hence, the contribution of this factor during the non-heating period was computed as well.

    Table 3 also shows the scores for each sampling site (n = 6). Similar to indoor VOC levels, the highest IAPi scores were found in industrial, rural, and urban sites in descending order. Only one of the sampling sites (Urb-2) was classified as "good" in terms of total IAPi, building/environmental conditions and present occupants' habits, while the other sampling sites fell mostly in "moderate" indoor air quality class. One of the sampling sites located in an industrial region was "bad" in terms of occupants' habits during the heating season (Ind-1). Finally, IAPi value of both sites located in industrial areas were in the "bad" class for the heating period (i.e. Ind 1-2) and in "bad" class in Ind-1 for the annual IAPi value.

    Table 2. Ranges and scoring criteria for the basic indoor air pollution index (IAPi).
    Building/Environmental factors IAPi range Scoring
    Floor number 0-2 0: > 2nd floor 1: 2nd flor 2: ≤ 1st floor
    Distance to traffic 1-3 1: less 2: moderate 3: much
    Room area 1-2 1: > 15 m2 2: < 15 m2
    Flooring material type 0-1 0: concrete 1: wooden
    Last floor covering time 0-1 0: > 1 year 1: < 1 year
    Carpeting amount in the room 1-3 1: < 1/4 of the room 2: 1/4-1/2 of the room 3: > 1/2 of the room
    Wooden product amount in the room 1-3 1: < 1/4 of the room 2: 1/4-1/2 of the room 3: > 1/2 of the room
    Last wall painting time 0-1 0: > 1 year 1: ≤ 1 year
    Total score for building/environmental factors 4-16 4 12 +11
    Occupants' habits
    House cleaning frequency 1-2 1: maximum once a week 2: more than once a week
    Pesticide usage 0-1 0: no 1: yes
    Naphthalane/air freshener usage 0-1 0: no 1: yes
    Cooking fuel type 0-2 0: natural gas 1: butane-propane cylinder
    Heating fuel type 0-2 0: natural gas 1: coal 2: coal & wood
    Average daily ventilation duration 0-1 0:> 3 h d−1 1:>3 h d−1
    Total score for occupants' habits 1-9 1 7 +2
    Total score 5-25
     | Show Table
    DownLoad: CSV
    Table 3. Indexing Criteria and IAPi of the sampling sites.
    Building/Environmental factors Range Class code Urb-1 Urb-2 Urb-3 Rur-1 Ind-1 Ind-2
    Good 4-7 0 6
    Moderate 10-12 1 10 10 9 10 12
    Bad 13-16 2
    Occupants' habits
    Good 1-3 0 3* 3 (3*) 2 (2*)
    Moderate 4-6 1 4 5 (4*) 6* 5 (4*)
    Bad 7-9 2 8
    IAPi (total score)
    Good 5-10 0 9 (9*)
    Moderate 11-16 1 14 (13*) 12 (12*) 14 (13*) 16*
    Bad 17-25 2 (17*) 17
    *refers to non-heating season score
     | Show Table
    DownLoad: CSV

    3.3. Association between observed VOC levels and indoor air pollution index (IAPi)

    In addition to indexing the indoor environments according to their building/environmental conditions as well as occupants' habits, associations between IAPi and observed VOC levels were examined. For this aim, average levels of annual, heating-period, and non-heating period TVOC, benzene, toluene, and xylenes gathered from a total of 112 samples (see Table 4) and scores of building/environmental factors, occupants' habits, and total factors were analyzed with bivariate regression analyses. Table 5 shows the bivariate regression analysis results with correlation coefficients (r2). In terms of correlations between the scores and observed VOC levels, there were strong correlations (r2 > 0.7) for annual/heating period TVOC levels and annual benzene levels with total score/occupants' habits; with xylenes during annual/non-heating period occupants' habits; and with occupants' habits and toluene levels during the non-heating period and benzene levels for all three periods. As mentioned earlier, heating activity both at the sampling sites and around their outer surroundings can influence the observed VOC composition and therefore it might have a masking effect on the correlations. Thus, in addition to annual averages, non-heating period averages of the VOCs were used for the regression analyses as well. Finally, only a good relationship was found (r2 > 0.6) between building/environmental factors and heating period TVOC levels. Hence, these results indicate that the most significant factors affecting observed VOC levels are occupants' habits, other than building/environmental factors.

    Another study, investigating the source apportionments of indoor VOC levels used a factor analysis approach and found a significant effect of VOC including product usage indoors as a main mechanism, but they did not include the occupants' habits directly in the analyses [6].


    3.4. Basic numerical methods for classification of indoor environments according to observed indoor VOC levels (IAPvoc)

    Three basic numerical methods, namely SOM, the rounded average approach, and the maximum value approach were used here to classify the indoor environments in terms of VOC abundance (IAPvoc). Similar to IAPi approach, the higher score the environment has, the worse the IAQ of the environment regarding VOC pollution (higher IAPvoc score). The other similarity of IAPvoc approach with IAPi approach is that indoor environments were classified into three classes: "good", "moderate, and "bad".

    Table 4. Number of data used for the data analysis at each sampling point.
    Sampling point N
    Urb-1 12
    Urb-2 10
    Urb-3 12
    Rur-1 10
    Ind-1 11
    Ind-2 12
    Total 114
     | Show Table
    DownLoad: CSV
    Table 5. R2 values between the average VOC concentrations and IAPi scores.
    TVOC Total score Occupants' habits Building/Environmental factors
    annual concentrations 0.94 (0.92*) 0.87 0.40
    heating concentrations 0.73 0.37 0.61
    non-heating concentrations 0.36* 0.93* 0.17
    Benzene
    annual concentrations 0.67 (0.71*) 0.81 0.18
    heating concentrations 0.64 0.73 0.18
    non-heating concentrations 0.55* 0.73* 0.30
    Toluene
    annual concentrations 0.41 (0.46*) 0.68 0.04
    heating concentrations 0.23 0.56 0.01
    non-heating concentrations 0.57* 0.84* 0.09
    Xylenes
    annual concentrations 0.49 (0.54*) 0.70 0.08
    heating concentrations 0.08 0.01 0.26
    non-heating concentrations 0.48* 0.84* 0.05
    *refers to non-heating season scores; n = 114; R2 ≥ 0.70 were underlined.
     | Show Table
    DownLoad: CSV

    Figure 6 depicts the SOM results. In Figure 6 (a) represents SOM Neighbor Weight Distances that displays air quality classes, and (B) represents SOM Sample Hits that display how sample unit presents 10 × 10 neuron units. Evaluated classes (Class 1: "good", class 2 = "moderate", class 3 = "bad") are visualized on Figure 6. Similar classes are shown with the same color. Where the disparity of light color shows small differences, the disparity of darker color shows huge differences. Figure 7 shows the classes of indoor environments (n = 6) estimated by SOM, the rounded average, and the maximum value approaches for (A) annual; (B) heating period; and (C) non-heating period average values of TVOC and BTX. According to Figure 7, all three approaches are strictly consistent for Ind-1 sampling site during all sampling periods, while SOM underestimated the class of environment in urban sampling sites as annual averages, and also SOM overestimated the class of the environment in Rur-1 for annual average and heating period; in Urb-2 during non-heating period; and in Urb-1 during heating period. When we compare the rounded average and the maximum value approaches, they were in good agreement except in Urb-2 during the heating period and in Ind-2 during the non-heating period.

    Figure 6. Clustering of Data According to SOM at the sampling sites for IAPvoc: A) SOM Neighbor Weight Distances, B) SOM Sample Hits (10 × 10 matrix; the lighter nests: Class 1 "good", grey nests: Class 2 "moderate", the darkest nests: Class 3 "bad").

    Table 6 shows the summary of the estimation of the three methods as a pairwise comparison. As can be seen from both Figure 7 and Table 6, the rounded average and the maximum value approaches have 88.9% agreement while classifying the indoor environments according to their VOC composition. When it comes to the SOM classification, only around 30% match was found with the other two methods. Also, the environments were in better classes with SOM methods than other two methods.

    Table 6. Comparison table of three IAPvoc methods* for the estimated classes of the sampling sites.
    Method pairs N n (%n)
    Identical Worse in SOM Worse in rounded average Worse in maximum value
    SOM vs. Rounded average 18 6 (33.4) 4 (22.2) 8 (44.4) -
    SOM vs. Maximum value 18 5 (27.8) 4 (22.2) - 9 (50)
    Rounded average vs. Maximum value 18 16 (88.9) - 0 2 (11.1)
    *annual, heating period, and non-heating period IAPvoc scores were used (N = 6 sampling sites × 3 periods = 18; n: number of data for each group, %n: frequency of each group (n/N × 100))
     | Show Table
    DownLoad: CSV
    Figure 7. IAPvoc classes estimated by SOM, the rounded average, and the maximum value methods at the sampling sites for target VOC concentrations: A) annual avg, B) heating period, and C) non-heating period.

    4. Conclusions

    Levels of TVOC, benzene, toluene, and xylenes were measured in six different homes in Çanakkale city, Turkey throughout a year. Levels of TVOC and BTX were found to be higher in industrial areas, particularly in Ind-1 sampling site, compared to other towns. VOC levels were higher during the heating season except in the industrial region, probably due to more frequent ventilation with the outdoor air where the enhanced photochemistry of toluene/xylenes might have occurred due to additional industrial sources.

    IAPi for the factors affecting IAQ can be a useful tool in typical indoor environments with natural ventilation in terms of assessing the availability of potential sources and triggering factors for VOCs. This study showed that both building/environmental factors and occupants' habits contributed to observed indoor VOC levels. Among those factors, occupants' habits were found to be the most significant group according to the bivariate regression results (r2 > 0.70).

    Ecological data analysis is very complex. Similar to IAPi, the IAPvoc approach was used to classify the indoor environments into three classes (good-moderate-bad) according to their VOC composition. For this aim, three different basic numerical methods were utilized here. Among them, SOM is a very powerful tool for analyzing environmental data. Although SOM is frequently used for water and surface water modelling etc., it is not commonly used for indoor air pollution modelling. In this study, SOM gave an overall class for IAQ. With the SOM method, the sampled environments were in better classes than the other two methods, which were the rounded average and the maximum value methods. The rounded average and the maximum value approaches were in a very good agreement (88.9%) in terms of classifying the indoor environments according to their VOC composition. When it comes to the SOM classification, only around 30% similarity was found with other two methods.

    Even so, SOM is a good visualization method but less data may not provide good results. Since plenty of studies are available in the literature measuring the VOCs in different types of indoor environments, there is no indexing study for the potential sources and/or factors of observed VOCs. Although there are some limitations in this study such as small sample size for indoor environments, low number of individual VOCs and occupants, it gives a basic but start for further studies. Increasing the number of sampled environments and occupants would result in more precise and significant conclusions for numerical analyses than those obtained from this study.


    Acknowledgments

    This study was financially supported by The Scientific and Technological Council of Turkey (TUBITAK). Project no: 112Y059. The authors also thank to Mrs. Tülay Sabaz Orak and Mrs. Catherine Yigit for proofreading.


    Conflict of Interest

    All authors declare no conflicts of interest in this paper


    [1] Maragakis NJ, Rothstein JD (2001) Glutamate Transporters in Neurologic Disease. Arch Neurol 58: 365-370.
    [2] Stanton PK (1996) Long term depression, Long term potentiation, and the sliding threshold for long-term synaptic plasticity. Hippocampus 6: 35-42.
    [3] Meldrum BS (2000) Glutamate as a neurotransmitter in the brain: review of physiology and pathology. J Nutr 130: 1007S-1015.
    [4] Fagg GE, Foster AC (1983) Amino acid neurotransmitters and their pathways in the mammalian central nervous system. Neurosci 9: 701–719.
    [5] Cotman CW, Monaghan DT, Ottersen OP, et al. (1987) Anatomical organization of excitatory amino acid receptors and their pathways. Trends Neurosci 10: 273-280. doi: 10.1016/0166-2236(87)90172-X
    [6] Peinado JM, Mora F (1986) Glutamic acid as a putative transmitter of the interhemispheric corticocortical connections in the rat. J Neurochem 47: 15498-16003.
    [7] Hlinák Z, Gandalovicová D, Krejcí I (2005) Behavioural deficits in adult rats treated neonatally with glutamate. Neurotox Teratol 27: 465-473. doi: 10.1016/j.ntt.2005.03.006
    [8] Onaolapo OJ, Onaolapo AY, Akanmu MA, et al. (2016) Evidence of alterations in brain structure and antioxidant status following ‘low-dose’ monosodium glutamate ingestion. Pathophysiol (in press). http://dx.doi.org/10.1016/j.pathophys.2016.05.001
    [9] Geha RS, Beiser A, Ren C, et al. (2000) Review of Alleged Reaction to Monosodium Glutamate and Outcome of a Multicenter Double-Blind Placebo-Controlled Study. J Nutr 130: 1058S-1062S.
    [10] National Academy of Sciences, National Research Council (1979) The 1977 Survey of the Industry on the Use of Food Additives: Estimates of Daily Intake. Vol.3, Washington D.C.: National Academy Press.
    [11] Beyreuther K, Biesalski HK, Fernstrom JD, et al. (2007) Consensus meeting: mono sodium glutamate—An update. Eur J Clin Nutr 61: 304-313. doi: 10.1038/sj.ejcn.1602526
    [12] Shi Z, Luscombe-Marsh ND, Wittert GA, et al. (2010) Monosodium Glutamate is not associated with obesity or a greater prevalence of weight gain over 5 years: findings from the Jiangsu Nutrition Study. Br J Nutr 104: 457-463. doi: 10.1017/S0007114510000760
    [13] Insawang T, Selmi C, Cha'on U, et al. (2012) Monosodium glutamate (MSG) intake is associated with the prevalence of metabolic syndrome in a rural Thai population. Nutr Metab (Lond) 9: 50.
    [14] Collison KS, Makhoul NJ, Inglis A, et al (2010) Dietary trans-fat combined with monosodium glutamate induces dyslipidemia and impairs spatial memory. Physiol Behav 99: 334-342. doi: 10.1016/j.physbeh.2009.11.010
    [15] Olvera-Cortes E, Lopez-Vazquez MA, Beas-Zarate C, et al. (2005) Neonatal exposure to MSG disrupts place learning ability in adult rats. Pharmacol Biochem Behav 82: 247-251.
    [16] Wong PT, Neo H, Teo WL, et al. (1997) Deficits in water escape performance and alterations in hippocampal cholinergic mechanisms associated with neonatal monosodium glutamate treatment in mice. Pharmacol Biochem Behav 57: 383-388.
    [17] Frieder B, Grimm VE (1984) Prenatal monosodium glutamate (MSG) treatment given through the mother's diet causes behavioral deficits in rat offspring. Int J Neurosci 23: 117-126.
    [18] Narayanan SN, Paval KJ, Nayak S (2010) Effect of ascorbic acid on monosodium glutamate induced neurobehavioral changes in periadolescent rats. Bratisel Lek Listy 111: 247-252.
    [19] Choi DW (1988) Glutamate neurotoxicity and diseases of the nervous system. Neuron 1: 623-634. doi: 10.1016/0896-6273(88)90162-6
    [20] Ankarcrona M, Dypbukt JM, Bonfoco E (1995) Glutamate induced neuronal death: a succession of necrosis or apoptosis depending on mitochondrial function. Neuron 15: 961-973. doi: 10.1016/0896-6273(95)90186-8
    [21] Olney JW, Wozniak DF, Farber NB (1997) Excitotoxic neurodegeneration in Alzheimer disease. New hypothesis and new therapeutic strategies. Arch Neurol 54: 1234-1240.
    [22] Lemkey-Johnston N, Reynolds WA (1974) Nature and extent of brain lesions in mice related to ingestion of monosodium glutamate: a light and electron microscope study. J Neuropathol Exper Neurol 3: 74-97.
    [23] Takasaki Y, Matsuzawa Y, Iwata S, et al. (1979) Toxicological studies of monosodium L-glutamate in rodents; relationship between routes of administration and neurotoxicity. In: Glutamic Acid: Advances in Biochemistry (Filer LJ. Garattini S. Kare MR. Reynolds WA. Wurtman RJ. Eds.): Raven Press, New York. 255-275.
    [24] Onaolapo OJ, Onaolapo AY (2011) Acute low dose monosodium glutamate retards novelty induced behaviours in male Swiss albino mice. JNBH 3: 51-56.
    [25] Onaolapo AY, Onaolapo OJ, Mosaku TJ, et al. (2013) Histological Study of the Hepatic and Renal Effects of Subchronic Low Dose Oral Monosodium Glutamate in Swiss Albino Mice. BJMMR 3: 294-306. doi: 10.9734/BJMMR/2013/2065
    [26] Falalieieva TM, Kukhars'ky? VM, Berehova TV (2010) Effect of long-term monosodium glutamate administration on structure and functional state of the stomach and body weight in rats. Fiziol Zh 56: 102-110.
    [27] Hodgson AS (2001) Some facts about monosodium glutamate (MSG). Foods Nutr 8.
    [28] Ma MX, Chen YM, He J, et al. (2007) Effects of morphine and its withdrawal on Y-maze spatial recognition memory in mice. Neurosci 147: 1059-1065. doi: 10.1016/j.neuroscience.2007.05.020
    [29] Jung WR, Kim HG, Kim KL (2008) Ganglioside GQ1b improves spatial learning and memory of rats as measured by the Y-maze and the Morris water maze tests. Neurosci Letters 439: 220-225. doi: 10.1016/j.neulet.2008.05.020
    [30] Itoh J, Nabaeshima T, Kameyama T (1990) Utility of an elevated plus maze for the evaluation of nootropics, scopolamine and electro convulsion shock. Psychopharmacol 101: 27-33. doi: 10.1007/BF02253713
    [31] Onaolapo OJ, Onaolapo AY, Akinola OR, et al. (2014) Dexamethasone regimens alter spatial memory and anxiety levels in mice. J Behav Brain Sc 4: 159-167. doi: 10.4236/jbbs.2014.44019
    [32] Onaolapo OJ, Onaolapo AY, Awe EO, et al. (2013) Oral artesunate-amodiaquine combination causes anxiolysis and impaired cognition in healthy Swiss mice. IOSR:JPBS 7: 97-102.
    [33] San Gabriel AM, Maekawa T, Uneyama H, et al. (2007) mGluR1 in the fundic glands of rat stomach. FEBS Lett581: 11191123.
    [34] Uneyama H, Niijima A, San Gabriel A, et al. (2006) Luminal amino acid sensing in the rat gastric mucosa. Am J Physiol Gastrointest Liver Physiol291:G1163-1170.
    [35] Li X, Staszewski L, Xu H, et al. (2002)Human receptors for sweet and umami taste. Proc Natl Acad Sci USA99: 4692-4696.
    [36] Kondoh T, Mallick H.N, Torii K(2009) Activation of the gut-brain axis by dietary glutamate and physiologic significance in energy homeostasis. Am J Clin Nutr 90:832S837S.
    [37] Niijima A (2002) Reflex effects of oral, gastrointestinal and hepatoportal glutamate sensors on vagal nerve activity. J Nutr130:971S973S.
    [38] Kondoh T, Torii K (2008) MSG intake suppresses weight gain, fat deposition and plasma leptin levels in male Sprague–Dawley rats. Physiol Behav 95: 135-144.
    [39] Oleksandra AS, Oleksandr VVT, Falalyeyeva TM, et al. (2014) The efficacy of probiotics for monosodium glutamate-induced obesity: Dietology concerns and opportunities for prevention EPMA J 5(1): 2.
    [40] Tordoff MG, Aleman TR, Murphy MC (2012) No effects of monosodium glutamate consumption on the body weight or composition of adult rats and mice. Physiol Behav 107: 338-345. doi: 10.1016/j.physbeh.2012.07.006
    [41] Park CH, Choi SH, Piaoa Y, et al. (2000) Glutamate and aspartate impair memory retention and damage hypothalamic neurons in adult mice. Toxicol Lett 115: 117-125. doi: 10.1016/S0378-4274(00)00188-0
    [42] Kubo T, Kohira R, Okano T, et al. (1993) Neonatal glutamate can destroy the hippocampal CA1 structure and impair discrimination learning in rats. Brain Res 616: 311-314. doi: 10.1016/0006-8993(93)90223-A
    [43] Morris RG, Anderson E, Lynch GS, et al (1986) Selective impairment of learning and blockade of long-term potentiation by an N methyl-D-aspartate receptor antagonist, AP5. Nature 27: 774-776.
    [44] Onaolapo OJ, Onaolapo AY, Mosaku TJ, et al. (2012) Elevated plus maze and Y-maze behavioural effects of subchronic, oral low dose monosodium glutamate in Swiss albino mice IOSR-JPBS 3: 21-27.
    [45] Shivasharan BD, Nagakannan P, Thippeswamy BS, et al. (2013) Protective Effect of Calendula officinalis L. Flowers Against Monosodium Glutamate Induced Oxidative Stress and Excitotoxic Brain Damage in Rats. Indian J Clin Biochem 28: 292-298.
    [46] Meyer-Gerspach AC, Suenderhauf C, Bereiter L, et al. (2016) Gut Taste Stimulants Alter Brain Activity in Areas Related to Working Memory: a Pilot Study. Neurosignals 24: 59-70. doi:10.1159/000442612.
    [47] Buzescu A, Cristea NA, Chiriac A, et al. (2014) Experimental research on the interactions between some anxiolytics and dietary sodium monoglutamate. Acta Medica Marisiensis 60: 260-264.
    [48] Cortese BM, Phan KL (2005) The role of glutamate in anxiety and related disorders. CNS Spectr 10: 820. doi: 10.1017/S1092852900010427
    [49] Simon AB, Gorman JM (2006) Advances in the treatment of anxiety: targeting glutamate. Neuro Rx 3: 57-68. doi: 10.1016/j.nurx.2005.12.005
    [50] Letizia B, Maricla T, Sara C, et al. (2008) Magnetic resonance imaging volumes of the hippocampus in drug-naive patients with post-traumatic stress disorder without comorbidity conditions. J Psych Res 42: 752-762. doi: 10.1016/j.jpsychires.2007.08.004
    [51] Amiel JM, Mathew SJ (2007) Glutamate and anxiety disorders. Curr Psychiatry Rep 9: 278-283. doi: 10.1007/s11920-007-0033-7
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