AIMS Bioengineering, 2016, 3(3): 389-399. doi: 10.3934/bioeng.2016.3.389

Research article

Export file:

Format

  • RIS(for EndNote,Reference Manager,ProCite)
  • BibTex
  • Text

Content

  • Citation Only
  • Citation and Abstract

Switchgrass storage effects on the recovery of carbohydrates after liquid hot water pretreatment and enzymatic hydrolysis

1 Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR, USA
2 Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK, USA
3 University of Nebraska-Lincoln, 218 L.W. Chase Hall, P.O. Box 830726, Lincoln, NE 68583-0726 USA

Perennial grasses that would be used for bioenergy and bioproducts production will need to be stored for various periods of time to ensure a continual feedstock supply to a bioprocessing facility. The effects of storage practices on grass composition and the response of grasses to subsequent bioprocesses such as pretreatment and enzymatic hydrolysis needs to be understood to develop the most efficient storage protocols. This study examined the effect of outdoor storage of round switchgrass bales on composition before and after liquid hot water pretreatment (LHW) and enzymatic hydrolysis. This study also examined the effect of washing LHW pretreated biomass prior to enzymatic hydrolysis. It was determined that switchgrass composition after baling was stable. As expected, glucan and lignin contents increased after LHW due to decreases in xylan and galactan. Washing biomass prior to enzymatic hydrolysis reduced saccharification, especially in samples from the interior of the bale, by at least 5%.
  Figure/Table
  Supplementary
  Article Metrics

References

1. Emery IR, Mosier NS (2012) The impact of dry matter loss during herbaceous biomass storage on net greenhouse gas emissions from biofuels production. Biomass Bioenerg 39: 237–246.    

2. Shi J, Chinn MS, Sharma-Shivappa RR (2008) Microbial pretreatment of cotton stalks by solid state cultivation of Phanerochaete chrysosporium. Bioresource Technol 99: 6556–6564.    

3. Sanderson MA, Egg RP, Wiselogel AE (1997) Biomass losses during harvest and storage of switchgrass. Biomass Bioenerg 12: 107–114.    

4. Djioleu AC, Sverzut CB, Martin EM, et al. (2014) Effects of harvest and storage of switchgrass on the recovery of carbohydrates during dilute acid pretreatment and enzymatic hydrolysis. Forage Grazinglands 12.

5. Sluiter J, Sluiter A (2010) Summative Mass Closure. Laboratory Analytical Procedure Review and Integration: Feedstocks. NREL/TP-510-48087. Golden, Colorado: National Renewable Energy Laboratory.

6. Sluiter A, Hyman D (2008) Determination of total solids in biomass and total dissolved solids in liquid process samples. NREL Technical Report No. NREL/TP-510-42621. Golden, Colorado: National Renewable Energy Laboratory.

7. Hyman D, Sluiter A, Crocker D, et al. (2007) Determination of Acid Soluble Lignin Concentration Curve by UV-Vis Spectroscopy; Laboratory Analytical Procedure (LAP). NREL Technical Report NREL/TP-510-42620. Golden, Colorado: National Renewable Energy Laboratory.

8. Sluiter A, Hames B, Ruiz R, et al. (2005) Determination of sugars, byproducts, and degradation products in liquid fraction process samples Golden, CO: National Renewable Energy Laboratory.

9. Frederick N, Zhang N, Djioleu A, et al. (2013) The effect of washing dilute acid pretreated poplar biomass on ethanol yields. In: Chandel AK, Silva SS, editors. Sustainable degradation of lignocellulosic biomass—techniques, applications, and commercialization. Rijeka, Croatia: InTech, 105–117.

10. Dogaris I, Karapati S, Mamma D, et al. (2009) Hydrothermal processing and enzymatic hydrolysis of sorghum bagasse for fermentable carbohydrates production. Bioresource Technol 100: 6543–6549.    

11. Fisher RA (1960) The Design of Experiments. New York: Hafner.

12. Wiselogel AE, Agblevor FA, Johnson DK, et al. (1996) Compositional changes during storage of large round switchgrass bales. Bioresource Technol 56: 103–109.    

13. Laser M, Schulman D, Allen SG, et al. (2002) A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanol. Bioresource Technol 81: 33–44.    

14. Perez JA, Ballesteros I, Ballesteros M, et al. (2008) Optimizing liquid hot water pretreatment conditions to enhance sugar recovery from wheat straw for fuel-ethanol production. Fuel 87: 3640–3647.    

15. Mosier N, Wyman C, Dale B, et al. (2005) Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresource Technol 96: 673–686.

16. Suryawati L, Wilkins MR, Bellmer DD, et al. (2009) Effect of hydrothermolysis process conditions on pretreated switchgrass composition and ethanol yield by SSF with Kluyveromyces marxianus IMB4. Process Biochem 44: 540–545.    

17. Werner K, Pommer L, Brostrom M (2014) Thermal decomposition of hemicelluloses. J Anal Appl Pyrol 110: 130–137.    

Copyright Info: © 2016, Mark R. Wilkins, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

Download full text in PDF

Export Citation

Article outline

Show full outline
Copyright © AIMS Press All Rights Reserved