This study evaluated the color stability, bioactive compounds, and antioxidant activity of roselle calyx powders dissolved at different pHs. The results showed that purple roselle calyx powders revealed better color than red roselle, and foam-mat dried powders were better than the conventionally dried powder. The best solution without pH adjustment was found in foam-mat dried purple calyces. pH values significantly affected color stability and quality, bioactive compounds, and antioxidant activity of roselle powder solutions. The lightness, redness, and yellowness of the solutions at different pH values ranged from 27.00 to 74.45, from 0.70 to 52.20, and from 3.9 to 47.50, respectively. The non-adjusted pH solution had a lightness of 33.80–49.05, redness of 19.15–42.45, and yellowness of 7.10–18.35. The color quality, indicated by color intensity at different pHs, ranged from 0.03 to 15.24; the non-adjusted pH solution had a color intensity of 1.37–33.48, revealing that pH decreased color quality. Retention of phenolics and flavonoids was 8.02%–99.69% and 0.00%–81.05%, respectively, indicating the instability of bioactive compounds. Roselle antioxidant activity was observed at different pHs, ranging from 0.00 to 1.11 mg Trolox equivalent (TE)/g for DPPH (2, 2-diphenyl-1-picrylhydrazyl) scavenging activity and 0.46–104.16 mg TE/g for the ABTS [2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] assay. Acidic pHs preserved better all dependent parameters. In practice, the pH value should be considered when using roselle calyx powders in food products. This study used a solution as a model for a watery food system. Suitable products to use roselle calyx powders are water-based products with an acidic pH, such as syrups, yogurts and yogurt-based products, fermented milk, and sour-taste beverages. Roselle calyx powders are suitable to use as coloring agents or as bioactive compounds to provide healthy functional properties. The use of foam-mat dried powders is suggested due to the better antioxidant activity and color preservation than conventionally dried powder.
Citation: Teti Estiasih, Jatmiko Eko Witoyo, Khofifah Putri Wulandari, Fadhillah Dwi Juniati, Widiastuti Setyaningsih, Hanifah Nuryani Lioe, Miguel Palma, Kgs Ahmadi, Hamidie Ronald Daniel Ray, Elya Mufidah. Stability comparison of conventional and foam-mat red and purple dried roselle calyces powder as a function of pH[J]. AIMS Agriculture and Food, 2025, 10(1): 177-198. doi: 10.3934/agrfood.2025010
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This study evaluated the color stability, bioactive compounds, and antioxidant activity of roselle calyx powders dissolved at different pHs. The results showed that purple roselle calyx powders revealed better color than red roselle, and foam-mat dried powders were better than the conventionally dried powder. The best solution without pH adjustment was found in foam-mat dried purple calyces. pH values significantly affected color stability and quality, bioactive compounds, and antioxidant activity of roselle powder solutions. The lightness, redness, and yellowness of the solutions at different pH values ranged from 27.00 to 74.45, from 0.70 to 52.20, and from 3.9 to 47.50, respectively. The non-adjusted pH solution had a lightness of 33.80–49.05, redness of 19.15–42.45, and yellowness of 7.10–18.35. The color quality, indicated by color intensity at different pHs, ranged from 0.03 to 15.24; the non-adjusted pH solution had a color intensity of 1.37–33.48, revealing that pH decreased color quality. Retention of phenolics and flavonoids was 8.02%–99.69% and 0.00%–81.05%, respectively, indicating the instability of bioactive compounds. Roselle antioxidant activity was observed at different pHs, ranging from 0.00 to 1.11 mg Trolox equivalent (TE)/g for DPPH (2, 2-diphenyl-1-picrylhydrazyl) scavenging activity and 0.46–104.16 mg TE/g for the ABTS [2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] assay. Acidic pHs preserved better all dependent parameters. In practice, the pH value should be considered when using roselle calyx powders in food products. This study used a solution as a model for a watery food system. Suitable products to use roselle calyx powders are water-based products with an acidic pH, such as syrups, yogurts and yogurt-based products, fermented milk, and sour-taste beverages. Roselle calyx powders are suitable to use as coloring agents or as bioactive compounds to provide healthy functional properties. The use of foam-mat dried powders is suggested due to the better antioxidant activity and color preservation than conventionally dried powder.
The hippocampus plays a central role to form new episodic memory in various species including humans [1]. The hippocampal neurons seem to process variety of information,such as spatial location [2],temporal information [3],and emotional state [4] within specific episodes [5]. However,the critical mechanism how to sustain a piece of specific memory or what associates the memory fragment each other is still largely unknown.
Since selective blockade of long-term potentiation(LTP)induction by NMDA receptor antagonist impairs hippocampal learning [6],LTP has been considered as a cellular model of hippocampal memory [7]. In 2006,in vivo field EPSC recording study showed that hippocampal learning induces LTP in CA1 region of hippocampus [8]. Further,we revealed that learning-dependent synaptic delivery of AMPA receptors into the CA3-CA1 synapses is required for hippocampal learning [9]. Since there is no tetanus electrode in brain,endogenous trigger and /or the mechanism inducing the learning-dependent LTP were unknown.
I hypothesized acetylcholine(ACh)as an endogenous trigger of LTP,since the ACh release in the hippocampus increases during learning or exploration. Moreover,without electrode for tetanus stimulation,bath treatment of ACh agonist not only induces specific bursts [10] but also forms LTP in CA1 region of hippocampal slices [11]. Based on the hypothesis,I found that i)cholinergic trigger drives learning-dependent synaptic plasticity at excitatory and inhibitory synapses,and ii)learning requires the diversity of synaptic inputs in CA1 pyramidal neurons [12].
ACh seems to be necessary to strengthen the information-specific tagged CA1 synapses [13],depicted by hippocampal-cortical networks [14].
A number of studies suggest that ACh plays an important role in orchestrating majorhippocampal functions(Figure 1). In behavioural studies,ACh release increases during learning [15,16,17] and is positively correlated with learning performance [18,19]. Bilateral injections of scopolamine into the dorsal hippocampus impair spatial learning ability [20],suggesting that muscarinic ACh receptors mediate the formation of spatial memory. At the network level,ACh generates a theta rhythm [21] that modulates the induction of long-term potentiation(LTP)in hippocampal CA1 neurons [22]. Studies exploring a genetic deficiency of muscarinic ACh receptors(M1 or M2)further show the impairment of LTP in the CA1 region [23,24]. At the cellular level,both pyramidal and non-pyramidal neurons in the hippocampal CA1 area receive direct cholinergic afferents mediated by muscarinic receptors [25,26,27]. In vitro studies showed that bath application of carbachol,a cholinergic agonist,induces LTP in CA1 pyramidal neurons without electrical stimulus,suggesting that ACh in the hippocampus plays a principal role in the synaptic plasticity of the CA1 pyramidal neurons [11]. Furthermore,a recent study revealed an intracellular mechanism of ACh: focal activation of muscarinic ACh receptors in one CA1 pyramidal neuron induces Ca2+ release from inositol 1,4,5-trisphosphate-sensitive stores to induce LTP [28].
Figure 1. Schematic illustration of septo-hippocampal cholinergic neurons in rats. Exposure to serious episode induces ACh release in the hippocampus that activates hippocampal functions(Mitsushima et al,2008). I know,you remember where you were and what you were doing when serious events occur.
ACh in the hippocampus not only enhances plasticity at CA1 synapses,but also contribute to adult neurogenesis in the dentate gyrus,that is required for contextual memory [29]. Neurotoxic lesions of forebrain cholinergic neurons or long-term scopolamine treatment decreases the number of newborn cells in the dentate gyrus [30,31].
Although it is well established that cholinergic inputs from the medial septum modulate learning and memory,evidence for the cholinergic regulation of learning-induced synaptic plasticity is lacking. To investigate learning-induced synaptic modification in the hippocampus,we used the IA task(Figure 2). In this paradigm,rats are allowed to cross from an illuminated box to a dark box where an electric foot shock is delivered. Thus,rats learn to avoid the dark box and stay in the lighted one,which they would normally not prefer [8,9]. The tendency to avoid the dark box therefore indicates the acquisition of contextual memories. The rats avoided entering the dark box when it was associated with a mild electric shock(IA-trained),but not those given foot shock without any contextual experience(unpaired),or those allowed to simply explore the experimental cage(walk-through). Untrained control rats were kept in their home cages and were not exposed to the IA apparatus.
Figure 2.(a)Schema of inhibitory avoidance(IA)task. On the day of IA training,rats were placed in the light side of the box. When rats moved into the dark side box,we closed the door and applied electrical foot shock(1.6 mA)for 2 sec. The rats were returned to the home cage soon after the shock.(b)Even one training,rats well remember the episode,spending much longer time in the light box after training [12].
Cholinergic neurons within the basal forebrain provide the major projection to the neocortex and hippocampus [32]. Cortical regions receive cholinergic inputs mainly from the nucleus basalis magnocellularis(NBM)or the diagonal b and of Broca,whereas the hippocampus receivescholinergic inputs mostly from the medial septum and horizontal limb of the diagonal b and of
Broca [32]. Because the cholinergic projections are necessary to maintain learning and
memory [33,34],we hypothesized that in vivo monitoring of ACh release in the hippocampus is necessary to elucidate learning function. To measure ACh release,we have performed in vivo microdialysis studies in freely moving male rats. Briefly,a microdialysis probe with a semi-permeable membrane(0.5 mm in length)was inserted into a specific brain area via a surgically pre-implanted guide cannula. We perfused the inside of the membrane with artificial cerebrospinal fluid,and assayed ACh in dialysates using a high-performance liquid chromatography system. As a result,we observed long-lasting(≈60 min)ACh release in CA1 in IA-trained rats but not in untrained,unpaired,or walk-through controls(Figure 3)[12].
Figure 3.In vivoACh levels in the rat hippocampal CA1 region under different learning conditions.(a)Locations of the in vivo microdialysis probe in CA1.(b)Extracellular ACh levels increased significantly during IA training,and remained high for 60 min. In the unpaired or walk-through control animals,the ACh level increased,but only transiently [12]. Squares indicate the timing of the behavioral task. Error bars indicate ± sem.
Although it is well established that cholinergic inputs from the medial septum modulate learning and memory,evidence for the cholinergic regulation of learning-induced synaptic plasticity is lacking. By combining HSV-mediated in vivo gene delivery with in vitro patch-clamp recordings,we reported that contextual learning drives GluA1-containing AMPA receptors into hippocampal CA3-CA1 synapses. Double IA-training using two different contexts further drove AMPA receptors into the CA3-CA1 synapses [35]. More importantly,the synaptic delivery is required for contextual learning,since bilateral expression of AMPA receptor delivery blocker(MPR-DD)successfully impaired the contextual learning [9].
To further examine the learning-dependent synaptic plasticity,we recorded miniature EPSC(mEPSC at -60 mV) and miniature IPSC(mIPSC at 0 mV)from the same CA1 neuron under the presence of TTX(0.5 µM). Although control rats(untrained,unpaired,or walk through)show small mEPSC and mIPSC amplitudes,IA trained rats show significantly higher mEPSC and mIPSC amplitudes with much wider variation(Figure 4). These results suggest that each CA1 neuron has different excitatory or inhibitory synaptic inputs with wide electrophysiological variation.
Figure 4.A hour after the training,we prepared acute brain slices to analyze synaptic inputs in each CA1 neuron using patch clamp method. Each dot represents mean miniature EPSC and miniature IPSC responses in each CA1 neuron. The results suggest that IA training enhanced electrophysiological diversity of excitatory and inhibitory synapses. Muscarinic M1 ACh receptor antagonist blocked the training-dependent diversity of mEPSCs. In contrast,nicotinic a7 ACh receptor antagonist blocked the diversity of mIPSCs [12](. Red crosses represent means ± sem.).
Since I hypothesized that ACh is an intrinsic trigger of the synaptic plasticity,cholinergic receptor antagonist was microinjected into the CA1 neurons 15 min before the contextual learning. Microinjection of muscarinic M1 receptor antagonist(pirenzepine)into the CA1 successfully blocked the learning-dependent increase in mEPSC amplitude but not mIPSC amplitude. Conversely,microinjection of nicotinic a7receptor antagonist(methyllycaconitine)successfully blocked the learning-dependent increase in mIPSC amplitude but not mEPSC amplitude. In behaving rats,bilateral microinjections of pirenzepine or methyllycaconitine into CA1 successfully block the learning.
These results suggest that i)cholinergic trigger drives learning-dependent synaptic plasticity at excitatory and inhibitory synapses and ii)learning requires the diversity of synaptic inputs in CA1 pyramidal neurons [12]. Based on the results,I hypothesized that the diversity probably depicts cell-specific outputs processing experienced episodes after training(Figure 5). The mechanism of synaptic tagging at inhibitory synapses should be revealed in future.
Figure 5.Current hypothesis of learning & memory. Note that each CA1 neuron expresses barcode-like output after learning. Acetylcholine triggers learning-dependent synaptic plasticity of excitatory and inhibitory synapses,inducing electrophysiological diversity of excitatory and inhibitory synapses. To process experienced episodes,the diversity probably depends on information-specific-tagged CA1 synapses depicted by hippocampal-cortical networks.
We recently monitored in vivo multiple-unit spike activity of CA1 neurons before,during,and after exposure to a strong episode using male rats. Although spontaneous firing rate was low in habituated home cage,spontaneous high frequency firing suddenly observed for seconds during or soon after the strong episode. Then,minutes after the episode,short term but high frequency ripple-like(on/off)synchronized firing was clearly observed and sustained [36]. These observations provide crucial evidence supporting our hypothesis of learning and memory(Figure 5).
To determine a possible location of contextual memory,we made acute brain slices in four different CA1 areas(dorsal right,dorsal left,ventral right and ventral left)in untrained or IA-trained rats. We found that IA training induced synaptic plasticity in both side of dorsal hippocampus,but not in ventral hippocampus [37]. Non-stationary noise-analysis techniques further revealed that IA training significantly increased the number of open channels in dorsal hippocampus,but not in ventral hippocampus. These results suggest that learning-dependent synaptic plasticity occur in dorsal hippocampus bilaterally,but not in ventral hippocampus [38]. Considering our previous study to block AMPA receptor delivery [9],encoded contextual information seems to locate in both side of dorsal hippocampus.
To determine whether the encoding of context or retrieval induces synaptic change,we made acute brain slices in 6 different timing after IA training. We found that i)the encoding of context rather than retrieval induces both excitatory and inhibitory synaptic change and ii)the encoding quickly induces the plastic change within 10 min after the training [39].
We recently reported the developmental change in hippocampal ACh levels and contextual learning. Extracellular ACh levels in the dorsal hippocampus are low in juveniles,but increase significantly in adults. Simultaneous monitoring of ACh levels and spontaneous locomotor activity further demonstrated the development of ACh release. Although both juvenile and adult rats exhibited significant correlations between ACh levels and spontaneous activity,juveniles exhibited much more spontaneous activity than adults when they showed equivalent ACh levels. Similarly,low contextual learning performance in juveniles significantly increased,demonstrating a developmental trajectory of hippocampal function. In this report,we further revealed a developmental relationship between contextual learning and ACh level in the hippocampus [Freezing(sec)= 1171 ACh(pmol/20 min)+ 36].
More importantly,the ACh release in juveniles seems to be important to development of hippocampus and learning,since long-term treatment of scopolamine after weaning specifically impaired contextual learning without changing pain sensitivity,emotional state,and spontaneous activity. The findings,together with previous reports,lead to the hypothesis that juveniles require more spontaneous activity than adults to activate hippocampal functions. Our findings support the notion that every boy and girl requires sufficient spontaneous play in parks or nature to promote brain activity as well as physical activity [40].
In humans,aging seems to attenuate the ACh levels. Moreover,a reduction in ACh synthesis is known as a common feature of Alzheimer's disease [41,42,43]. The disease is the most common form of dementia [44] and is frequently accompanied by insomnia,poor concentration,and day night confusion [45,46]. The centrally active acetylcholinesterase inhibitor(donepezil)is effective in not only mild,but also moderate to severe cases [47,48],proving the importance of endogenous ACh in humans. We found neonatal sexual differentiation of the septo-hippocampal cholinergic system,suggesting sex-specific clinical strategies for Alzheimer’s disease [3]. Underst and ing the further detailed mechanism of ACh-triggered learning-dependent plasticity is essential for real improvements in therapy.
I declare no conflicts of interest in this paper.
| [1] | El Bayani GF, Marpaung NLE, Simorangkir DAS, et al. (2018) Anti-inflammatory effects of Hibiscus sabdariffa Linn. on the IL-1β/IL-1ra ratio in plasma and hippocampus of overtrained rats and correlation with spatial memory. Kobe J Med Sci 64: E73–E83. |
| [2] |
Izquierdo-Vega JA, Arteaga-Badillo DA, Sánchez-Gutiérrez M, et al.(2020) Organic acids from Roselle (Hibiscus sabdariffa L.)-A brief review of its pharmacological effects. Biomedicines 8: 1–16. https://doi.org/10.3390/biomedicines8050100 doi: 10.3390/biomedicines8050100
|
| [3] |
Sapian S, Ibrahim Mze AA, Jubaidi FF, et al. (2023) Therapeutic Potential of Hibiscus sabdariffa Linn. in attenuating cardiovascular risk factors. Pharmaceuticals 16: 1–25. https://doi.org/10.3390/ph16060807 doi: 10.3390/ph16060807
|
| [4] | Islam A, Jamini TS, Islam M, et al. (2016) Roselle: A functional food with high nutritional and medicinal values. Fundam Appl Agric 1: 44–49. |
| [5] |
Tan SL, Sulaiman R (2020) Color and rehydration characteristics of natural red colorant of foam-mat dried Hibiscus sabdariffa L. powder. Int J Fruit Sci 20: 89–105. https://doi.org/10.1080/15538362.2019.1605557 doi: 10.1080/15538362.2019.1605557
|
| [6] |
Hamrouni-Sellami I, Rahali FZ, Rebey IB, et al. (2013) Total phenolics, flavonoids, and antioxidant activity of sage (Salvia officinalis L.) plants as affected by different drying methods. Food Bioprocess Technol 6: 806–817. https://doi.org/10.1007/s11947-012-0877-7 doi: 10.1007/s11947-012-0877-7
|
| [7] |
Roshanak S, Rahimmalek M, Goli SAH (2016) Evaluation of seven different drying treatments in respect to total flavonoid, phenolic, vitamin C content, chlorophyll, antioxidant activity and color of green tea (Camellia sinensis or C. assamica) leaves. J Food Sci Technol 53: 721–729. https://doi.org/10.1007/s13197-015-2030-x doi: 10.1007/s13197-015-2030-x
|
| [8] |
Tajudin NHA, Tasirin SM, Ang WL, et al. (2019) Comparison of drying kinetics and product quality from convective heat pump and solar drying of Roselle calyx. Food Bioprod Process 118: 40–49. https://doi.org/10.1016/j.fbp.2019.08.012 doi: 10.1016/j.fbp.2019.08.012
|
| [9] |
Djaeni M, Kumoro AC, Sasongko SB, et al. (2018) Drying rate and product quality evaluation of roselle (Hibiscus sabdariffa L.) calyces extract dried with foaming agent under different temperatures. Int J Food Sci 2018: 9243549. https://doi.org/10.1155/2018/9243549 doi: 10.1155/2018/9243549
|
| [10] |
Liu Y, Liu Y, Tao C, et al. (2018) Effect of temperature and pH on stability of anthocyanin obtained from blueberry. J Food Meas Charact 12: 1744–1753. https://doi.org/10.1007/s11694-018-9789-1 doi: 10.1007/s11694-018-9789-1
|
| [11] |
Sasongko SB, Djaeni M, Utari FD (2019) Kinetic of anthocyanin degradation in roselle extract dried with foaming agent at different temperatures. Bull Chem React Eng Catal 14: 320–325. https://doi.org/10.9767/bcrec.14.2.2875.320-325 doi: 10.9767/bcrec.14.2.2875.320-325
|
| [12] |
Wu HY, Yang KM, Chiang PY (2018) Roselle anthocyanins: antioxidant properties and stability to heat and pH. Molecules 23: 1357. https://doi.org/10.3390/molecules23061357 doi: 10.3390/molecules23061357
|
| [13] |
Estiasih T, Witoyo JE, Runtung GKM, et al. (2024) The characteristics of foam-mat dried red and purple roselle calyces powder prepared with commercial emulsifiers as the foaming agents. CYTA-J Food 22: 2356847. https://doi.org/10.1080/19476337.2024.2356847 doi: 10.1080/19476337.2024.2356847
|
| [14] |
Enaru B, Drețcanu G, Pop TD, et al. (2021) Anthocyanins: Factors affecting their stability and degradation. Antioxidants 10: 1967. https://doi.org/10.3390/antiox10121967 doi: 10.3390/antiox10121967
|
| [15] |
Friedman M, Jürgens HS (2000) Effect of pH on the stability of plant phenolic compounds. J Agric Food Chem 48: 2101–2110. https://doi.org/10.1021/jf990489j doi: 10.1021/jf990489j
|
| [16] |
Mattioli R, Francioso A, Mosca L, et al. (2020) Anthocyanins: A comprehensive review of their chemical properties and health effects on cardiovascular and neurodegenerative diseases. Molecules 25: 3809. https://doi.org/10.3390/molecules25173809. doi: 10.3390/molecules25173809
|
| [17] |
Maisto M, Marzocchi A, Ciampaglia R, et al. (2024) Effects of food-derived antioxidant compounds on in vitro heavy metal intestinal bioaccessibility. Antioxidants 13: 610. https://doi.org/10.3390/antiox13050610 doi: 10.3390/antiox13050610
|
| [18] |
Shruthi VH, Ramachandra CT, Nidoni U, et al. (2017). Physico-chemical, nutritional and functional properties of roselle (Hibiscus sabdariffa L.). Int J Curr Microbiol Appl Sci 6: 1–7. https://doi.org/10.20546/ijcmas.2017.612.347 doi: 10.20546/ijcmas.2017.612.347
|
| [19] |
Rocha IDC, Bonnlaender B, Sievers H, et al. (2014). Hibiscus sabdariffa L.—A phytochemical and pharmacological review. Food Chem 165: 424–443. https://doi.org/10.1016/j.foodchem.2014.05.002. doi: 10.1016/j.foodchem.2014.05.002
|
| [20] |
Marpaung AM, Paramaputri A (2023) UV-visible light spectra of Clitoria ternatea L. flower extract during aqueous extraction and storage. Int Food Res J. 30: 764–773. https://doi.org/10.47836/ifrj.30.3.18 doi: 10.47836/ifrj.30.3.18
|
| [21] |
Samadi S, Fard FR (2020) Phytochemical properties, antioxidant activity and mineral content (Fe, Zn and Cu) in Iranian produced black tea, green tea and roselle calyces. Biocatal Agric Biotechnol 23: 101472. https://doi.org/10.1016/j.bcab.2019.101472 doi: 10.1016/j.bcab.2019.101472
|
| [22] |
Huang J, Hu Z, Chin Y, et al. (2023) Improved thermal stability of roselle anthocyanin by co-pigmented with oxalic acid: preparation, characterization and enhancement mechanism. Food Chem 410: 135407. https://doi.org/10.1016/j.foodchem.2023.135407 doi: 10.1016/j.foodchem.2023.135407
|
| [23] |
Ifie I, Marshall LJ, Ho P, et al. (2016) Hibiscus sabdariffa (Roselle) extracts and wine: phytochemical profile, physicochemical properties, and carbohydrase inhibition. J Agric Food Chem 64: 4921–4931. https://doi.org/10.1021/acs.jafc.6b01246. doi: 10.1021/acs.jafc.6b01246
|
| [24] |
Baliyan S, Mukherjee R, Priyadarshini A, et al. (2022) Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa. Molecules 27: 1326. https://doi.org/10.3390/molecules27041326 doi: 10.3390/molecules27041326
|
| [25] |
Thaipong K, Boonprakob U, Crosby K, et al. (2006) Comparison of ABTS, DPPH, FRAP, and ORAC Assays for estimating antioxidant activity from guava fruit extracts. J Food Anal 19: 669–675. https://doi.org/10.1016/j.jfca.2006.01.003 doi: 10.1016/j.jfca.2006.01.003
|
| [26] | Shruthi VH, Ramachandra CT (2019) Roselle (Hibiscus sabdariffa L.) calyces: A potential source of natural colour and its health benefits. In: Deka SC, Seth D, Hulle VRS (Eds.), Food bioactive: Functionalities and applications in human health, New York: Apple Academic Press, 169–190. https://doi.org/10.1201/9780429242793 |
| [27] |
Song W, Yuan Q, Xie Y, et al. (2024) Formulation and characterization of nanocapsules loaded with roselle anthocyanins extract and enhancement of anthocyanins bioaccessibility. Food Chem 459: 140446. https://doi.org/10.1016/j.foodchem.2024.140446 doi: 10.1016/j.foodchem.2024.140446
|
| [28] |
Yang D, Xu HX, Guo YX, et al. (2023) Chemical profile of roselle extract and its inhibitory activities on three digestive enzymes in vitro and in vivo. Int J Biol Macromol 253: 126902. https://doi.org/10.1016/j.ijbiomac.2023.126902 doi: 10.1016/j.ijbiomac.2023.126902
|
| [29] |
Khoo HE, Azlan A, Tang ST, et al. (2017) Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food Nutr Res 61: 1361779. https://doi.org/10.1080/16546628.2017.1361779 doi: 10.1080/16546628.2017.1361779
|
| [30] | Aishah B, Nursabrina M, Noriham A, et al. (2013), Anthocyanins from Hibiscus sabdariffa, Melastoma malabathricum and Ipomoea batatas and its color properties. Int Food Res J 20: 827–834. |
| [31] | Marpaung A, Tjahjadi K (2019) The analysis of monomeric anthocyanin by pH differential method is not appropriate for certain anthocyanins. In: Proceedings of the 16th ASEAN Food Conference (16th AFC 2019)—Outlook and Opportunities of Food Technology and Culinary for Tourism Industry, 26–30. https://doi.org/10.5220/0009985400002964 |
| [32] |
Dorris MR, Voss DM, Bollom MA, et al. (2018) Browning index of anthocyanin‐rich fruit juice depends on pH and anthocyanin loss more than the gain of soluble polymeric pigments. J Food Sci 83: 911–921. https://doi.org/10.1111/1750-3841.14106 doi: 10.1111/1750-3841.14106
|
| [33] |
Gao Q, Li Y, Li Y, et al. (2022) Antioxidant and prooxidant activities of phenolic acids commonly existed in vegetables and their relationship with structures. Food Sci Technol 42: e07622. https://doi.org/10.1590/fst.07622 doi: 10.1590/fst.07622
|
| [34] |
Nowak M, Tryniszewski W, Sarniak A, et al. (2022) Concentration dependence of anti-and pro-oxidant activity of polyphenols as evaluated with a light-emitting Fe2+-Egta-H2O2 System. Molecules 27: 3453. https://doi.org/10.3390/molecules27113453 doi: 10.3390/molecules27113453
|
| [35] |
Rajashekar CB (2023) Dual role of plant phenolic compounds as antioxidants and prooxidants. Am J Plant Sci 14: 15–28. https://doi.org/10.4236/ajps.2023.141002 doi: 10.4236/ajps.2023.141002
|
| [36] |
Wu HY, Yang KM, Chiang PY (2018) Roselle anthocyanins: antioxidant properties and stability to heat and pH. Molecules 23: 1357. https://doi.org/10.3390/molecules23061357 doi: 10.3390/molecules23061357
|
| [37] |
Horbowicz M, Kosson R, Grzesiuk A, et al. (2008). Anthocyanins of fruits and vegetables - their occurrence, analysis and role in human nutrition. Veg Crops Res Bull 68: 5–22. https://doi.org/10.2478/v10032-008-0001-8 doi: 10.2478/v10032-008-0001-8
|
| [38] |
Punyachareonnon, Deerattrakul V, Luepong K (2021) The Influence of pH, temperature and time on dyeing of silk fabric by black bean anthocyanin-rich extract as colorant. Prog Color Color Coatings 14: 179–186. https://doi.org/10.30509/pccc.2021.81715 doi: 10.30509/pccc.2021.81715
|
| [39] |
Millinia BL, Mashithah D, Nawatila R, et al. (2024) Microencapsulation of roselle (Hibiscus sabdariffa L.) anthocyanins: Effects of maltodextrin and trehalose matrix on selected physicochemical properties and antioxidant activities of spray-dried powder. Future Foods 9: 100300. https://doi.org/10.1016/j.fufo.2024.100300 doi: 10.1016/j.fufo.2024.100300
|
| [40] |
Idham Z, Putra NR, Aziz AHA, et al. (2022) Improvement of extraction and stability of anthocyanins, the natural red pigment from roselle calyces using supercritical carbon dioxide extraction. J CO2 Util 56: 101839. https://doi.org/10.1016/j.jcou.2021.101839 doi: 10.1016/j.jcou.2021.101839
|
| [41] |
Jiang T, Mao Y, Sui L, et al. (2019) Degradation of anthocyanins and polymeric color formation during heat treatment of purple sweet potato extract at different pH. Food Chem 274: 460–470. https://doi.org/10.1016/j.foodchem.2018.07.141 doi: 10.1016/j.foodchem.2018.07.141
|
| [42] |
Pasquet PL, Julien-David D, Zhao M, et al. (2024) Stability and preservation of phenolic compounds and related antioxidant activity from agro-food matrix: effect of pH and atmosphere. Food Biosci 57: 103586. https://doi.org/10.1016/j.fbio.2024.103586 doi: 10.1016/j.fbio.2024.103586
|
| [43] |
Wang Y, Xing L, Zhang J, et al. (2023) Determination of endogenous phenolic compounds in honey by HPLC-MS/MS. LWT 183: 114951. https://doi.org/10.1016/j.lwt.2023.114951 doi: 10.1016/j.lwt.2023.114951
|
| [44] | Krungkri W, Areekul V (2019) Effect of heating condition and pH on stability of total phenolic content and antioxidant activities of samui (Micromelum minutum) extract. In: Proceedings of the 16th ASEAN Food Conference (16th AFC 2019)—Outlook and Opportunities of Food Technology and Culinary for Tourism Industry. Bali, Indonesia, 15–32. https://doi.org/10.5220/0009980801260132 |
| [45] |
Chen YT, Kao WT, Lin KW (2008) Effects of pH on the total phenolic compound, antioxidative ability and the stability of dioscorin of various yam cultivars. Food Chem 107: 250–257. https://doi.org/10.1016/j.foodchem.2007.08.017 doi: 10.1016/j.foodchem.2007.08.017
|
| [46] |
Rosales-Murillo SS, Sánchez-Bodón J, Hernández Olmos SL, et al. (2024) Anthocyanin-loaded polymers as promising nature-based, responsive, and bioactive materials. Polymers 16: 163. https://doi.org/10.3390/polym16010163 doi: 10.3390/polym16010163
|
| [47] |
Cabrita L, Fossen T, Andersen ØM (2000) Colour and stability of the six common anthocyanidin 3-glucosides in aqueous solutions. Food Chem 68: 101–107. https://doi.org/10.1016/S0308-8146(99)00170-3 doi: 10.1016/S0308-8146(99)00170-3
|
| [48] |
Rakić V, Ulrih NP (2021) Influence of pH on color variation and stability of cyanidin and cyanidin 3-O-β-glucopyranoside in aqueous solution. CyTA-J Food 19: 174–182. https://doi.org/10.1080/19476337.2021.1874539 doi: 10.1080/19476337.2021.1874539
|
| [49] |
Sui X, Dong X, Zhou W (2014) Combined effect of pH and high temperature on the stability and antioxidant activity of two anthocyanins in aqueous solution. Food Chem 163: 163–170. https://doi.org/10.1016/j.foodchem.2014.04.075 doi: 10.1016/j.foodchem.2014.04.075
|
| [50] |
Rigolon TCB, Silva RRA, de Oliveira TV, et al. (2024) Exploring anthocyanins-polysaccharide synergies in microcapsule wall materials via spray drying: interaction characterization and evaluation of particle stability. Meas Food 13: 100126. https://doi.org/10.1016/j.meafoo.2023.100126 doi: 10.1016/j.meafoo.2023.100126
|
| [51] |
Tena N, Martín J, Asuero AG (2020) State of the art of anthocyanins: antioxidant activity, sources, bioavailability, and therapeutic effect in human health. Antioxidants 9: 451. https://doi.org/10.3390/antiox9050451 doi: 10.3390/antiox9050451
|
| [52] |
Floegel A, Kim DO, Chung SJ, et al. (2011) Comparison of ABTS/DPPH assays to measure antioxidant activity in popular antioxidant-rich US foods. J Food Compos Anal 24: 1043–1048. https://doi.org/10.1016/j.jfca.2011.01.008 doi: 10.1016/j.jfca.2011.01.008
|
| [53] |
Bayliak MM, Burdyliuk NI, Lushchak VI (2016) Effects of pH on antioxidant and prooxidant properties of common medicinal herbs. Open Life Sci 11: 298–307. https://doi.org/10.1515/biol-2016-0040 doi: 10.1515/biol-2016-0040
|
| [54] |
Sun HN, Mu TH, Xi LS (2017) Effect of pH, heat, and light treatments on the antioxidant activity of sweet potato leaf polyphenols. Int J Food Prop 20: 318–332. https://doi.org/10.1080/10942912.2016.1160410 doi: 10.1080/10942912.2016.1160410
|
| [55] |
Kungsuwan K, Singh K, Phetkao S, et al. (2014) Effect of pH and anthocycnin concentration on colour and antioxidant activity of Clitorea ternatea extract. Food Appl Biosci J 2: 31–46. https://doi.org/10.14456/fabj.2014.3 doi: 10.14456/fabj.2014.3
|
| [56] |
Yu L, Wu Y, Liu D, et al. (2022) The kinetic behavior of antioxidant activity and the stability of aqueous and organic polyphenol extracts from navel orange peel. Food Sci Technol 42 :e90621. https://doi.org/10.1590/fst.90621 doi: 10.1590/fst.90621
|
| [57] |
Kamiloglu S, Capanoglu E, Grootaert C, et al. (2015) Anthocyanin absorption and metabolism by human intestinal Caco2-cells—A review. Int J Mol Sci 16: 21555–21574. https://doi.org/10.3390/ijms160921555 doi: 10.3390/ijms160921555
|
| [58] |
Kudličková Z, Michalková R, Salayová A, et al. (2023) Design, synthesis, and evaluation of novel indole hybrid chalcones and their antiproliferative and antioxidant activity. Molecules 28: 6583. https://doi.org/10.3390/molecules2818658 doi: 10.3390/molecules2818658
|
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