Effect and mechanism of long non-coding RNA ZEB2-AS1 in the occurrence and development of colon cancer

Xu Guo; Yuanming Jing; Haizhou Lou; Qiaonv Lou; Xu Guo; Yuanming Jing; Haizhou Lou; Qiaonv Lou

doi:10.3934/mbe.2019408

Mathematical Biosciences and Engineering

2019, Volume 16, Issue 6: 8109-8120. doi: 10.3934/mbe.2019408

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Research article Special Issues

Effect and mechanism of long non-coding RNA ZEB2-AS1 in the occurrence and development of colon cancer

1.
Department of Anorectal Surgery, Dalian Municipal Central Hospital, Dalian, Liaoning 116033, China
2.
Department of General Surgery, Shaoxing People's Hospital (Shaoxing Hospital, Zhejiang University School of Medicine)
3.
Department of Medical Oncology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, China
4.
Department of Orthopedics, The Sixth People's Hospital of Cixi, Ningbo 315300, China

Received: 20 February 2019 Accepted: 10 July 2019 Published: 06 September 2019

ObjectiveClarify the expression changes, biological functions and related mechanisms of long non-coding RNA (lncRNA) ZEB2-AS1 in colon cancer tissues.

Methods The expression levels of ZEB2-AS1 in colon cancer tissues and adjacent tissues were detected by qRT-PCR and in situ hybridization methods. Cell biology experiments were performed to detect the proliferation, migration and apoptosis of colon cancer cells when the level of ZEB2-AS1 was overexpression or silencing. Then, Western blot was performed to analyze the effect of ZEB2-AS1 on the expression levels of β-catenin protein and related genes in the signal pathway.

Results We found that the expression level of ZEB2-AS1 in colon cancer tissues was significantly up-regulated compared with that in adjacent normal tissues. In colon cancer cell line of HCT8, overexpression of ZEB2-AS1 could promote cell proliferation and migration, while silencing ZEB2-AS1 would enhance cell apoptosis and inhibit proliferation. Study on the mechanism of ZEB2-AS1 showed that it could promote the expression of β-catenin, activate downstream genes to be transcribed and promote the occurrence and development of tumors.

Conclusion ZEB2-AS1 could promote colon cancer cell proliferation and inhibit apoptosis to promote the progression of colon cancer by upregulating the expression of β-catenin protein. ZEB2-AS1 may be a useful new target for treating colon cancer patients.

Keywords:

Citation: Xu Guo, Yuanming Jing, Haizhou Lou, Qiaonv Lou. Effect and mechanism of long non-coding RNA ZEB2-AS1 in the occurrence and development of colon cancer[J]. Mathematical Biosciences and Engineering, 2019, 16(6): 8109-8120. doi: 10.3934/mbe.2019408

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Abstract

ObjectiveClarify the expression changes, biological functions and related mechanisms of long non-coding RNA (lncRNA) ZEB2-AS1 in colon cancer tissues.

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