In plant science, we are currently at the dawn of an era, in which mathematical modeling and computational simulations will influence and boost tremendously the gain of new knowledge. However, for many plant scientists mathematical modeling is still rather dubious and is often negligently considered as an oversimplification of the real situation. The goal of this article is to provide a toolbox that allows first steps in the modeling of transport phenomena in plants. The provided framework is applied in the simulation of K+ uptake by cells via K+ channels. Historically, K+ uptake systems are divided into “high affinity” (e.g. H+-coupled K+ transporters) and “low affinity” (K+ channels) transporters. The computational cell biology studies presented here refute this separation. They show that K+ channels are in general uptake systems with “low” and “high affinity” components. The analyses clarify that constraints in wet-lab experiments usually mask the “high affinity” component. Consequently, the channels were widely assigned a “low affinity” component, only. The results presented here unmask the absurdity of the concept of “high- and low-affinity” transporters.
Citation: Ingo Dreyer. Plant potassium channels are in general dual affinity uptake systems[J]. AIMS Biophysics, 2017, 4(1): 90-106. doi: 10.3934/biophy.2017.1.90
Related Papers:
[1]
Wael S. Abu El Azm, Ramy Aldallal, Hassan M. Aljohani, Said G. Nassr .
Estimations of competing lifetime data from inverse Weibull distribution under adaptive progressively hybrid censored. Mathematical Biosciences and Engineering, 2022, 19(6): 6252-6275.
doi: 10.3934/mbe.2022292
[2]
Walid Emam, Ghadah Alomani .
Predictive modeling of reliability engineering data using a new version of the flexible Weibull model. Mathematical Biosciences and Engineering, 2023, 20(6): 9948-9964.
doi: 10.3934/mbe.2023436
[3]
M. E. Bakr, Abdulhakim A. Al-Babtain, Zafar Mahmood, R. A. Aldallal, Saima Khan Khosa, M. M. Abd El-Raouf, Eslam Hussam, Ahmed M. Gemeay .
Statistical modelling for a new family of generalized distributions with real data applications. Mathematical Biosciences and Engineering, 2022, 19(9): 8705-8740.
doi: 10.3934/mbe.2022404
[4]
M. Nagy, Adel Fahad Alrasheedi .
The lifetime analysis of the Weibull model based on Generalized Type-I progressive hybrid censoring schemes. Mathematical Biosciences and Engineering, 2022, 19(3): 2330-2354.
doi: 10.3934/mbe.2022108
[5]
Bo Dong, Alexey Luzin, Dmitry Gura .
The hybrid method based on ant colony optimization algorithm in multiple factor analysis of the environmental impact of solar cell technologies. Mathematical Biosciences and Engineering, 2020, 17(6): 6342-6354.
doi: 10.3934/mbe.2020334
[6]
Mahmoud El-Morshedy, Zubair Ahmad, Elsayed tag-Eldin, Zahra Almaspoor, Mohamed S. Eliwa, Zahoor Iqbal .
A new statistical approach for modeling the bladder cancer and leukemia patients data sets: Case studies in the medical sector. Mathematical Biosciences and Engineering, 2022, 19(10): 10474-10492.
doi: 10.3934/mbe.2022490
[7]
M. G. M. Ghazal, H. M. M. Radwan .
A reduced distribution of the modified Weibull distribution and its applications to medical and engineering data. Mathematical Biosciences and Engineering, 2022, 19(12): 13193-13213.
doi: 10.3934/mbe.2022617
[8]
Giuseppina Albano, Virginia Giorno, Francisco Torres-Ruiz .
Inference of a Susceptible–Infectious stochastic model. Mathematical Biosciences and Engineering, 2024, 21(9): 7067-7083.
doi: 10.3934/mbe.2024310
[9]
Fathy H. Riad, Eslam Hussam, Ahmed M. Gemeay, Ramy A. Aldallal, Ahmed Z.Afify .
Classical and Bayesian inference of the weighted-exponential distribution with an application to insurance data. Mathematical Biosciences and Engineering, 2022, 19(7): 6551-6581.
doi: 10.3934/mbe.2022309
[10]
Manal M. Yousef, Rehab Alsultan, Said G. Nassr .
Parametric inference on partially accelerated life testing for the inverted Kumaraswamy distribution based on Type-II progressive censoring data. Mathematical Biosciences and Engineering, 2023, 20(2): 1674-1694.
doi: 10.3934/mbe.2023076
Abstract
In plant science, we are currently at the dawn of an era, in which mathematical modeling and computational simulations will influence and boost tremendously the gain of new knowledge. However, for many plant scientists mathematical modeling is still rather dubious and is often negligently considered as an oversimplification of the real situation. The goal of this article is to provide a toolbox that allows first steps in the modeling of transport phenomena in plants. The provided framework is applied in the simulation of K+ uptake by cells via K+ channels. Historically, K+ uptake systems are divided into “high affinity” (e.g. H+-coupled K+ transporters) and “low affinity” (K+ channels) transporters. The computational cell biology studies presented here refute this separation. They show that K+ channels are in general uptake systems with “low” and “high affinity” components. The analyses clarify that constraints in wet-lab experiments usually mask the “high affinity” component. Consequently, the channels were widely assigned a “low affinity” component, only. The results presented here unmask the absurdity of the concept of “high- and low-affinity” transporters.
1.
Introduction
With the development and progress of manufacturing technology, modern products are designed with complex structures and have high reliability. However, for some high reliablity products, it is hard to obtain their failure data through traditional life tests within a short period of time. However, in many cases, degradation measurements can provide valuable information related to the failure mechanism of the product. Therefore, the product's reliability can be inferred and estimated through the degradation data of quality characteristics obtained [1].
In recent years, various kinds of degradation models have emerged and been studied. Some of these models are stochastic process models, mixed-effect models [2,3], and so on. Typical degradation models include the general degradation path models and stochastic process models. Meeker et al.[4] used a nonlinear regression model with mixed-effects to analyze constant-stress accelerated degradation test (CSADT) data. Shi and Meeker [5] discussed the accelerated destructive degradation test planning of a nonlinear regression model through the Bayesian method. The stochastic process models include the Wiener process model [6,7,8,9,10,11,12], the Gamma process model [13,14,15,16,17], and the inverse Gaussian (IG) process model [18,19,20,21,22]. Although Ye and Xie [23] have made a comprehensive study on degradation analysis of products with single quality characteristics (QC), reliability analysis of complex systems with two or more competing failure modes (e.g. sudden failure, degradation failure) is still a challenge.
The modeling and statistical analysis of competing risk data has increasingly become a hot issue in the field of reliability, and there are many literatures on the statistical analysis of competing risk data (or competing risk model), such as Nassr et al. [24], Ramadan et al. [25], Mohamed et al. [26] and Mohamed et al. [27]. Huang et al. [28] studied the optimal maintenance scheme of multi-dependent competitive degradation and shock processes. Fan et al. [29] used degradation-shock dependence to model the dependent competitive failure process and used Monte Carlo techniques to calculate the reliability of the system. Xu et al. [30] modeled competing failure with the bivariate Wiener degradation process. Wang et al. [31] proposed two semiparametric additive mean models for clustered panel count data, and estimated the regression parameters of interest by constructing the estimation equations. Mutairi et al. [32] studied the inverse Weibull model based on jointly type-II hybrid censoring samples through the Bayesian or non-Bayesian methods. Bhat et al. [33] discussed the properties and Bayesian estimation of the odd lindley power rayleigh distribution.
A motivating example of this study is provided by Huang and Askin [34]. Units in the system may fail when the solder/pad interface breaks due to fatigue [35], or when the electrical/optical signal drops to unacceptable levels due to aging degradation [36]. In this example, an electronic device failed caused by two independent failure elements: the light intensity degradation (soft failure), which is considered a degradation phenomenon, because at some common inspection times to observe and measure the light intensity of the device, the solder/bond pad interface breaks, which is regarded as a sudden failure (hard failure). The original data given in Tables 1 and 2 was measured under the same conditions. The degradation data is the ratio of the current brightness to the startup brightness. When this ratio is reduced by 60%, the product is assumed to fail. These two failure processes are both competitive and independent of each other. In a competitive failure model, the lifetime of the system is the least of many random lifetimes.
Table 1.
The ratio of current brightness and startup brightness.
To analyze the above data, Huang and Askin [34] assumed that both the sudden and degradation failures are modeled by a Weibull distribution and they discussed reliability analysis of this competing failure model. In their paper, they assumed that the population is homogeneous and describe the degradation process by assuming that the light intensity level at each inspection time follows a Weibull distribution whose shape and scale parameters are time dependent. Both of the shape and scale parameters are estimated by the degradation levels observed at each time. Zhao and Elsayed [37] assumed that the sudden failure time follows a Weibull distribution and the degradation failure process is modeled by a Brownian motion, and they used the maximum likelihood estimate (MLE) method to obtain the estimates of the model. Studies based on Huang and Askin [34] and Cha et al. [38] assume that the large heterogeneity observed during degradation is described in part by considering two distinct subpopulations and using least squares estimation to obtain the main reliability features.
Reliability is often closely related to system security. Hence, reliable inference procedures for competing failure model studies with small sample cases have become an important issue in reliability analysis. The challenge of providing reliable inference procedures based on small samples inspires us to explore interval estimation approaches for competing failure models. In this paper, we propose a Wiener-weibull competing failure model and develop the generalized pivotal quantity (GPQ) method to explore the interval estimation of system's reliability metrics under small sample case, and use the proposed model and method to analyze the data in the motivated example.
The rest of the paper is arranged as follows. In Section 2, we outline the Wiener-weibull competing failure model. In Section 3, the MLEs and inverse estimates (IEs) of model parameters are derived and the exact confidence intervals (ECIs), generalized confidence intervals (GCIs) for model parameters, and some important reliability metrics such as the pth quantile of lifetime, the reliability function, and the mean time to failure (MTTF) of system are developed. In Section 4, Monte Carlo techniques are used to examine the performance of the proposed GCIs in terms of the coverage percentage (CP) and average interval length (AL). In Section 5, an illustrative example is given to apply the proposed method. Finally, we summarize the article in Section 6.
2.
Wiener-weibull competing failure model
Supposed that the system is equipped with two groups of components: The first group contains a component, whose degradation process of quality characteristic is described as a stochastic process; the second group contains a component, whose lifetime is modeled by sudden failure. Moreover, the two components are operating independently. In this paper, we assume that the degradation process of quality characteristic for the first component is modeled by a Wiener process, and the lifetime of the second component due to sudden failure follows a Weibull distribution.
2.1. Wiener degradation process
It is assumed that the degradation path of the quality characteristics of the first component can be fitted using the Wiener process {X(t),t≥0}, denoted by
X(t)=μt+σB(t)
where μ and σ>0 are the drift and diffusion parameters, respectively, μ reflects the degradation rate, and B(⋅) denotes a standard Brownian motion. The Wiener process X(t) has the following properties:
● X(0)=0 is true with probability one.
● X(t|t≥0) has independent increments, that is, the increments X(t1)−X(t0),…,X(tn)−X(tn−1) are independent random variables for ∀0<t0<t1<⋯<tn−1<tn.
● Each increment, ΔX(t)=X(t+Δt)−X(t), follows a normal distribution N(0,σ2Δt).
The lifetime T1 of the first component is defined as the first hitting time of X(t) to a degradation threshold L. As is known to all, T1 follows the IG distribution IG(L/μ,L2/σ2). Therefore, the cumulative distribution function (CDF) of T1 is presented as
F1(t|μ,σ2)=Φ(μt−Lσ√t)+exp(2μLσ2)Φ(−μt+Lσ√t),t>0
(2.1)
where Φ(⋅) is the CDF of N(0,1) distribution.
2.2. Weibull sudden failure mode
Suppose that the lifetime T2 of the second component due to sudden failure follows a Weibull distribution, denoted by Weibull(β,η). The probability density function (PDF) of T2 is
f2(t|η,β)=βη(tη)β−1exp[−(tη)β],t>0
(2.2)
and the CDF of T2 is
F2(t|η,β)=1−exp[−(tη)β],t>0
(2.3)
where η>0 and β>0 are the scale and shape parameters, respectively.
Therefore, the lifetime of the system can be defined as T=min(T1,T2). The CDF of T and the reliability function of the system at time t are presented as
F(t)=F(t|μ,σ2,η,β)=1−[1−F1(t|μ,σ2)][1−F2(t|η,β)],
(2.4)
R(t)=P(T>t)=[1−F1(t|μ,σ2)][1−F2(t|η,β)]
(2.5)
respectively.
The MTTF of the system can be obtained by
MTTF=∫∞0[1−F1(t|μ,σ2)][1−F2(t|η,β)]dt
(2.6)
2.3. Data of competing failure model
Suppose that n systems are tested. Let ri denote the number of measurements for the first component of the ith system. The measurement times for the first component of the ith system ti,1,ti,2,…,ti,ri,1≤i≤n, are usually predetermined. Therefore, the degradation data is X={X(ti,j);i=1,2,…,n,j=1,2,…,ri}. Let Δti,jˆ=ti,j−ti,j−1, ΔXi,jˆ=X(ti,j)−X(ti,j−1) denote the degradation increment between ti,j−1 and ti,j, for i=1,2,…,n,j=1,2,…,ri. For convenience of expression, let T=∑ni=1∑rij=1Δti,j and M=∑ni=1ri denote the total test duration and the total number of measurements for the whole test, respectively. The sudden failure time of the second component for the ith system is Ti,2, i=1,2,…,n. Hence, the sudden failure times of the second component for n systems are T=(T1,2,T2,2,…,Tn,2).
The degradation data refers to 10 electronic devices whose degradation level (brightness) was measured at the same inspection times, with equal inspection time interval of 500 hours and the test duration up to 4,000 hours. Suppose that the degradation process {X(t);t≥0} is a Wiener process with drift parameter μ and diffusion parameter σ2. As the degradation level reaches to (or exceeds) the threshold level L, the device is considered as a fail. Where the degradation level is X(ti,j)=100−Yi(ti,j), Yi(ti,j) denotes the light intensity (in percentage relative to the original measurement) of the test unit i at time ti,j. The sudden failure data and the transformed degradation data for test units are given in Tables 2 and 3.
Table 3.
The transformed degradation data of luminance ratio for 10 test units.
In this section, we first give the MLE of parameters μ and σ2 for the Wiener degradation process. On basis of the MLEs of μ and σ2, the ECIs of μ and σ2 are obtained. Unfortunately, to get the confidence interval of the scale parameter η as intractable, we develop the GCIs of parameter η for the sudden failure model. It is well known that the pth quantile of system lifetime, the reliability function, and the MTTF of a system are three important characteristics in reliability analysis. However, it is intractable to obtain the ECIs of these three reliability characteristics, so we consider getting the GCIs of them.
3.1. Estimation for Wiener degradation model
Notice that the degradation increments of quality characteristic ΔXi,j are mutually independent, and ΔXi,j∼N(μΔti,j,σ2Δti,j) for i=1,2,…,n,j=1,2,…,ri. Hence, on basis of the degradation data X, the likelihood function is expressed as
Next, we will develop the ECIs for parameters μ and σ2. To derive the ECIs of μ and σ2, the following Theorem 1 is needed.
Theorem 3.1.Suppose that the degradation increments D={ΔXi,j;i=1,2,…,n,j=1,2,…,ri} are from the Wiener degradation process {X(t);t≥0} above. Let ˆμ=∑ni=1∑rij=1ΔXi,j/T, S2=1M−1∑ni=1∑rij=1(ΔXi,j−ˆμΔti,j)2Δti,j, then
1) ˆμ is an unbiased estimator of μ, and ˆμ∼N(μ,σ2/T);
2) S2 is an unbiased estimator of σ2, and (M−1)S2/σ2∼χ2(M−1);
3) S2 is independent of ˆμ.
Proof Notice that ∑ni=1∑rij=1ΔXi,j∼N(μT,σ2T), so ˆμ∼N(μ,σ2/T) is obvious. By telescoping ΔXi,j−μΔti,j as (ΔXi,j−ˆμΔti,j)+(ˆμΔti,j−μΔti,j), we have the following factorization:
respectively, where tγ(n) and χ2γ(n) are the lower γ percentiles of t and χ2 distributions with free degrees n, respectively.
3.2. Estimation for Weibull sudden failure model
In this subsection, for the Weibull sudden failure model, we will give the ECI of shape parameter β. Moreover, for point estimation, the IEs of parameters η and β are obtained. To construct the ECI for parameter β, the following Lemmas 1 and 2 tend to be useful.
Lemma 3.1.Suppose that Y1,Y2,…,Yn are independent identically distributed (i.i.d) random variables from Weibull distribution (2). Let Zi=(Yiη)β,i=1,2,…,n, then the Z1,Z2,…,Zn are independent standard exponential variables.
Lemma 1 is obvious, so here we neglect the detailed proof. □
Lemma 3.2.Given that Z1,Z2,…,Zn are standard exponential random variables and Z(1),Z(2),…,Z(n) are their order statistics, let ξ1=nZ(1),ξi=(n−i+1)(Z(i)−Z(i−1)),i=2,3,…,n; Si=∑ij=1ξj,U(i)=Si/Sn,i=1,2,…,n−1, and Sn=∑ni=1ξi, then
1) ξ1,ξ2,…,ξn are independent standard exponential random variables;
2) U(1)<U(2)<⋯<U(n−1) are the corresponding order statistics of uniform distribution U(0,1) with sample size n−1;
3) 2Sn follows the distribution χ2(2n).
Proof 1) As is known to all, the joint probability density function (JPDF) of (Z(1),Z(2),…,Z(n)) is
f(z1,z2,…,zn)=n!exp(−n∑i=1zi),0<z1<⋯<zn
Notice that ∑ni=1ξi=∑ni=1Z(i) and the Jacobian determinant is J=|∂(Z(1),Z(2),…,Z(n))∂(ξ1,ξ2,…,ξn)|=1n!, so the JPDF of (ξ1,ξ2,…,ξn) is obtained by
That is, ξ1,ξ2,…,ξn are independent standard exponential random variables.
2) From U(i)=Si/Sn,i=1,2,…,n−1, we know that ξ1=U(1)Sn,ξn=Sn−U(n−1)Sn and ξi=U(i)Sn−U(i−1)Sn,i=2,…,n−1. As the Jacobian determinant J=|∂(ξ1,ξ2,…,ξn)∂(U(1),…,U(n−1),Sn)|=Sn−1n, the JPDF of (U(1),…,U(n−1),Sn) is given by
f(u1,…,un−1,sn)=sn−1nexp(−sn),0<u1<⋯<un−1<1,sn>0
By marginal integral, the JPDF of (U(1),…,U(n−1)) is obtained by
Hence, U(1)<U(2)<⋯<U(n−1) are the corresponding order statistics of uniform distribution U(0,1) with sample size n−1.
3) Notice that Sn∼Ga(n,1), then we have 2Sn∼Ga(n,1/2)=χ2(2n). □
Next, we will construct pivotal quantities (PQs) for parameters β and η. Since the sudden failure data T is a sequence from the Weibull distribution (2), the corresponding order failure data is denoted by {T(1),2,T(2),2,…,T(n),2}. Based on Lemma 1, we know that the transformation {(Ti,2/η)β,i=1,2,…,n} is a sequence of standard exponential random variables. Thus, from Lemma 2, we have that
From Eq (3.1), we find that for Weibull distribution (2), W1 is a function with respect to the shape parameter β and free of the scale parameter η.
It is obvious that W1 is nonnegative. Notice that ∑n−1i=1logU(i)=∑n−1i=1logUi and Ui,i=1,2,…,n−1 are i.i.d random variables from the uniform distribution U(0,1). Moreover, we can prove the fact that W1∼χ2(2n−2).
Next, we will prove that W1 is strictly monotonic with respect to parameter β. Let Q(j,i)=(T(j),2/T(i),2)β. Note that
It can be observed from Eq (3.2) that W1 is strictly increasing with respect to parameter β, because Q(j,i) is strictly increasing (decreasing) for j>i (j<i). Hence, given a realization W1 from χ2(2n−2), there exists a unique solution g(W1,T) of β for Eq (3.1), then the PQ for parameter β is given as P1=g(W1,T). Therefore, an ECI of β for the Weibull distribution can be derived by the following Theorem 2.
Theorem 3.2.If T1,2,T2,2,…,Tn,2 are i.i.d random variables from Weibull distribution (2),
T(1),2,T(2),2,…,T(n),2 are the corresponding order statistics, then for any 0<γ<1,
[W−11(χ2γ/2(2n−2)),W−11(χ21−γ/2(2n−2))]
is a 1−γ level confidence interval of the shape parameter β. Here χ2γ(n) denotes the lower γ percentile of the χ2 distribution with freedom degrees n, and for t>0, W−11(t) is the solution of β for the equation W1(β)=t.
Notice that W1∼χ2(2n−2) and E(W1)=2(n−1). So, W1 converges to 2(n−1) with probability one. Let W1=2(n−1). Based on the following Eq (3.3), we can get the point estimator ˆβ of the shape parameter β
similar to the discussion above. Eq (3.3) also has a unique solution for parameter β.
Denote An=∑ni=1(Ti,2/η)β, so An∼Ga(n,1) and E(An)=n. Similarly, let An=n, and the corresponding point estimator ˆη of η is obtained by
ˆη=(∑ni=1Tˆβ(i),2n)1/ˆβ
(3.4)
The estimators obtained from Eqs (3.3) and (3.4) are named as IEs of parameters β and η, which was proposed in Wang [40].
3.3. GCIs for η,Tp,R(t0), and MTTF
In practical applications, some reliability metrics of a system, such as the pth quantile of lifetime, the reliability function R(t0), and the MTTF of system, may be of more importance than the model parameters. However, since these reliability metrics involve multiple parameters, it is intractable to obtain their exact confidence intervals. Therefore, we develop the GCIs for these reliability metrics.
Now we will construct the GPQ for the scale parameter η. Based on Lemmas 1 and 2, we know the quantity
W2=2ηβn∑i=1Tβ(i),2∼χ2(2n)
then the scale parameter η can be expressed as η=(2∑ni=1Tβ(i),2/W2)1/β. Recall that the PQ of β is P1=g(W1,T). Using the substitution method given by Weerahandi [41], we replace β by P1 in the expression of η and obtain the GPQ of parameter η
P2=(2n∑i=1TP1(i),2/W2)1/P1
(3.5)
It can be observed from Eq (2.3) that for Weibull sudden failure model, the reliability is R2(t0)=1−F2(t0|η,β). Using the substitution method, the GPQ of reliability R2(t0) is obtained by
R2(t0)=exp(−(t0P2)P1)
To derive the GPQ for reliability R1(t0) of the Wiener degradation model, we first construct the PQs of μ and σ.
Let
U=√T(ˆμ−μ)/σ,V=(M−1)S2/σ2
(3.6)
Obviously, U∼N(0,1) and V∼χ2(M−1) and they are mutually independent. Thus, μ and σ can be formulated as
μ=ˆμ−U√(M−1)S2/(VT),σ=√(M−1)S2/V
respectively, so the GPQs of μ and σ are obtained by
P3=ˆμ−UP4/√T,P4=√(M−1)S2/V
(3.7)
It should be pointed out that ˆμ and S2 are treated as known quantities in generalized inference [41]. Using the substitution method given in [41], the GPQ of R1(t0) is given by
R1(t0)=Φ(L−P3t0P4√t0)−exp(2P3LP24)Φ(−P3t+LP4√t0)
Based on Eqs (2.4)–(2.6), the GPQs for pth quantile of lifetime T, the reliability function, and the MTTF of a system can be obtained by
Let Pi,γ denote the γ percentile of Pi, then [Pi,γ/2,Pi,1−γ/2],i=2,5,6,7 are the 1−γ level GCIs of η,Tp,R(t0), and MTTF, respectively. The percentiles of Pi,i=2,5,6,7 can be acquired through the following Monte Carlo Algorithm.
Algorithm : The percentiles of η,Tp,R(t0), and MTTF.
Step 1 Given degradation data X and sudden failure data T, compute ˆμ,S2, and T.
Step 2 Generate W1∼χ2(2n−2),W2∼χ2(2n),U∼N(0,1), and V∼χ2(M−1), respectively.
Based on Eqs (3.1), (3.5), and (3.7), compute P1,P2,P3, and P4.
Step 3 Based on P1,P2,P3, and P4, using Eqs (3.8)–(3.10) to compute P5,P6, and P7
Step 4 Repeat steps (2) and (3) K times, then K values of Pi,i=2,5,6,7 are obtained, respectively.
Step 5 Sorting all Pi values in ascending order: Pi,(1)<Pi,(2)<…<Pi,(K),i=2,5,6,7, then the γ percentile of Pi is estimated by Pi,(γK).
4.
Simulation study
The Monte Carlo simulation technique is used to evaluate the performance of the proposed GCIs in the aspect of the CP and AL. Table 4 lists the different combinations of the model parameters μ,σ2,η,β and the threshold L for simulation study. Moreover, we take n=10,15,20, riˆ=r=8,10,12, Δti,jˆ=Δt=10, and K=10,000 in the simulation study. Based on 5000 replications, all the simulation results are provided in Tables 5–9.
Table 4.
Parameter settings for the simulation study.
Table 5.
R-Bias∗100 and R-MSE∗100 (in parentheses) of the point estimates for model parameters based on 5000 replications under parameter settings II, III, and IV.
To examine the performance of the point estimates of model parameters (μ,σ2,β,η), simulation studies were carried out in terms of relative-bias (R-Bias) and relative-mean square error (R-MSE) under the parameter setting II, III, and IV for (n,r)=(10,8),(15,10),(20,12). Motivated by Luo et. al. [42], the R-Bias and R-MSE are defined as:
R-Bias=|1nn∑i=1ˆθi−θθ|,R-MSE=1nn∑i=1(ˆθi−θ)2θ2
where θ and ˆθi are the true value and the estimate of a parameter, respectively.
Based on 5000 replications, the simulation results about the R-Bias and R-MSE of the proposed estimates for model parameters (μ,σ2,β,η) are provided in Table 5. It can be seen from Table 5 that both the R-Bias and R-MSE are small compared with the true values, and for given parameter settings as n and r increase, the R-MSEs decrease as expected. The simulation results show that these estimates perform well in aspect of both R-Bias and R-MSE. Hence, we recommend the proposed point estimates for model parameters (μ,σ2,β,η).
It is well known that the parametric bootstrap method is a classic approach to get confidence intervals for model parameters. In order to fully assess the performances of the GCIs, we also considered the bootstrap CIs for the proposed competing failure model. A comparative analysis is conducted between the CIs obtained by the GPQ method and the bootstrap-p method. Based on 5,000 bootstrap samples, the bootstrap-p CIs are obtained and provided in Tables 6–9.
It is observed from Tables 6–9 that the CPs of the proposed GCIs are quite close to the nominal levels, even in the small sample case. In many cases, the differences between the real CP and the nominal level are small, ranging in 1%, but the CPs obtained by the bootstrap-p method are away from the nominal levels for some parameters and quantities. In particular, we find that the bootstrap-p CIs of the scale parameter η are far below the nominal levels for all cases. In addition, from Tables 6 and 8 we also find that the bootstrap-p CIs perform bad for some quantities. For example, the CPs of lifetime quantile T0.1 and reliability function R(5) deviate from the nominal levels.
When the sample size n turns large, the CPs of the bootstrap-p CIs also near the nominal levels. Tables 6–9 report that, for fixed parameter settings, when n and r increase, the ALs become shorter for both GPQ and bootstrap-p CIs as expected. The comparison shows that the GCIs perform better than the corresponding bootstrap-p CIs in terms of CP. Hence, we recommend the GCIs for model parameter η and some quantities, such as Tp,R(t0),MTTF, particularly in the case of small sample.
5.
An illustrative example
In this section, we use the proposed Wiener-Weibull competing failure model and the GPQ method to analyze the real example provided by Huang and Askin [34]. The product is treated as a fail when the luminance ratio decreases by 60%. For convenience, we assumed that the original brightness is 100, so the threshold is L=60. The transformed degradation data of luminance ratio is presented in Table 3 and the weld interface fracture data (sudden failure data) is given in Table 2. Figure 1 shows the luminance ratio degradation paths of 10 test units. In this study, we use the Wiener process to model the degradation data in Table 3. Similar to Huang and Askin [34], the sudden failure data is also fitted by a Weibull distribution.
For point estimation, the MLEs of μ and σ2 are given by ˆμ=0.0310,ˆσ2=0.0076, respectively. The IEs of η and β are given by ˆη=1.0350×103,ˆβ=4.7684, respectively. Figure 2 shows the degradation paths, the sample average degradation path, and the fitted mean degradation path by model. It is clear that the estimates of the mean degradation paths provide good fitting for the sample average degradation paths. This means that it is reasonable to use the Wiener process to fit the luminance ratio degradation data. Given p=0.1,L=60, and t0=800(days), the pth percentile of lifetime, the reliability function at time t0, and the MTTF of the system are obtained by T0.1=642.8386(days), R(800)=0.7416, MTTF=943.5986(days), respectively. The point estimate of ^MTTF=22646(hours) is near to the estimate of MTTF (22,765 hours) provided by Huang and Askin [34].
Figure 2.
Sample average degradation path and the fitted mean degradation path.
For interval estimation, we use the GPQ method proposed in Section 3 to analyze the real dataset. As is known to all, some reliability metrics such as the pth percentile of lifetime T, the reliability function, and the MTTF of a system are more important than the model parameters in reliability analysis. However, as these metrics are very complicated, getting their interval estimations is usually difficult, so we developed the GCIs for them. Based on Eqs (3.8)–(3.10) and using the GPQ method, the GCIs of Tp,R(t0), and MTTF can be obtained. Take K=10000; the results are given in Table 10. According to the methods in Huang and Askin [34] and Cha et al. [38], they can only provide the point estimation for MTTF and not give its interval estimation.
Table 10.
The 90 and 95% CIs of model parameters and some reliability metrics.
In this paper, a competing failure model involving both degradation failure and sudden failure was studied by modeling degradation failure as a Wiener process and sudden failure as a Weibull distribution. For model parameters, the MLEs of μ,σ2, and the IEs of η,β were derived and the ECIs of parameters μ, σ2, and β are obtained.
In this study, the GPQ method was proposed to investigate the scale parameter and some reliability metrics of the competing failure model. By constructing the GPQs, the GCI of parameter η was developed. Using the substitution method, the GCIs for the reliability function, the pth percentile of the lifetime, and the MTTF of a system were also developed. Simulation studies were carried out to assess the performances of the proposed intervals. Simulation results reported that the proposed GCIs work well in aspect of the CP and are better than the corresponding bootstrap CIs. Finally, we applied the proposed model and the GPQ method to a real data example and found the ECIs and GCIs of model parameters and some reliability metrics.
Use of AI tools declaration
The authors declare they have not used Artificial Intelligence (AI) tools in the creation of this article.
Acknowledgements
The authors thank the Editor and the reviewers for their detailed comments and valuable suggestions, which helped improve the manuscript significantly. The work was supported in part by the National Social Science Foundation of China (21CTJ005), in part by the Talent Cultivation and Research Start-up Foundation of Anhui Polytechnic University (S022022014), in part by the Pre-research Project of the National Science Foundation of Anhui Polytechnic University (Xjky08201903), in part by the program for outstanding young talents in colleges and universities of Anhui Province (gxyqZD2022046), in part by the Excellent and Top-notch Personnel Cultivation Project of Universities (gxyq2022083), and in part by the Natural Science Research Project of Anhui Educational Committee (2023AH050927).
Conflict of interest
The authors declare that there is no conflict of interest.
References
[1]
G. Volkov A, B. Shtessel Y (2016) Propagation of electrotonic potentials in plants: Experimental study and mathematical modeling. AIMS Biophys 3: 358–379. doi: 10.3934/biophy.2016.3.358
[2]
Hedrich R, Salvador-Recatalà V, Dreyer I (2016) Electrical wiring and long-distance plant communication. Trends Plant Sci 21: 376–387. doi: 10.1016/j.tplants.2016.01.016
[3]
Jane Beilby M, Al Khazaaly S (2016) Re-modeling Chara action potential: I. from Thiel model of Ca2+ transient to action potential form. AIMS Biophys 3: 431–449.
[4]
Hills A, Chen ZH, Amtmann A, et al. (2012) OnGuard, a computational platform for quantitative kinetic modeling of guard cell physiology. Plant Physiol 159: 1026–1042. doi: 10.1104/pp.112.197244
[5]
Blatt MR, Wang Y, Leonhardt N, et al. (2014) Exploring emergent properties in cellular homeostasis using OnGuard to model K+ and other ion transport in guard cells. J Plant Physiol 171: 770–778. doi: 10.1016/j.jplph.2013.09.014
[6]
Gajdanowicz P, Michard E, Sandmann M, et al. (2011) Potassium (K+) gradients serve as a mobile energy source in plant vascular tissues. Proc Natl Acad Sci USA 108: 864–869. doi: 10.1073/pnas.1009777108
[7]
Foster KJ, Miklavcic SJ (2015) Toward a biophysical understanding of the salt stress response of individual plant cells. J Theor Biol 385: 130–142. doi: 10.1016/j.jtbi.2015.08.024
[8]
Schott S, Valdebenito B, Bustos D, et al. (2016) Cooperation through Competition-Dynamics and Microeconomics of a Minimal Nutrient Trade System in Arbuscular Mycorrhizal Symbiosis. Front Plant Sci 7: 912.
[9]
Epstein E, Rains DW, Elzam OE (1963) Resolution of dual mechanisms of potassium absorption by barley roots. Proc Natl Acad Sci USA 49: 684–692. doi: 10.1073/pnas.49.5.684
[10]
Hille B (2001) Ion channels of excitable membranes, 3rd Ed., Sunderland, MA: Sinauer.
[11]
Gajdanowicz P, Garcia-Mata C, Gonzalez W, et al. (2009) Distinct roles of the last transmembrane domain in controlling Arabidopsis K+ channel activity. New Phytol 182: 380–391. doi: 10.1111/j.1469-8137.2008.02749.x
[12]
Riedelsberger J, Sharma T, Gonzalez W, et al. (2010) Distributed structures underlie gating differences between the K in channel KAT1 and the Kout channel SKOR. Mol Plant 3: 236–245. doi: 10.1093/mp/ssp096
[13]
Garcia-Mata C, Wang J, Gajdanowicz P, et al. (2010) A minimal cysteine motif required to activate the SKOR K+ channel of arabidopsis by the reactive oxygen species H2O2. J Biol Chem 285: 29286–29294. doi: 10.1074/jbc.M110.141176
[14]
González W, Riedelsberger J, Morales-Navarro SE, et al. (2012) The pH sensor of the plant K+-uptake channel KAT1 is built from a sensory cloud rather than from single key amino acids. Biochem J 442: 57–63. doi: 10.1042/BJ20111498
[15]
Lefoulon C, Karnik R, Honsbein A, et al. (2014) Voltage-sensor transitions of the inward-rectifying K+ channel kat1 indicate a latching mechanism biased by hydration within the voltage sensor. Plant Physiol 166: 960–975. doi: 10.1104/pp.114.244319
[16]
Hedrich R, Bregante M, Dreyer I, et al. (1995) The voltage-dependent potassium-uptake channel of corn coleoptiles has permeation properties different from other K+ channels. Planta 197: 193–199.
[17]
Hedrich R, Moran O, Conti F, et al. (1995) Inward rectifier potassium channels in plants differ from their animal counterparts in response to voltage and channel modulators. Eur Biophys J 24: 107–115.
[18]
Becker D, Dreyer I, Hoth S, et al. (1996) Changes in voltage activation, Cs+ sensitivity, and ion permeability in H5 mutants of the plant K+ channel KAT1. Proc Natl Acad Sci USA 93: 8123–8128. doi: 10.1073/pnas.93.15.8123
Dietrich P, Dreyer I, Wiesner P, et al. (1998) Cation sensitivity and kinetics of guard-cell potassium channels differ among species. Planta 205: 277–287. doi: 10.1007/s004250050322
[21]
Dreyer I, Becker D, Bregante M, et al. (1998) Single mutations strongly alter the K+-selective pore of the K(in) channel KAT1. FEBS Lett 430: 370–376. doi: 10.1016/S0014-5793(98)00694-2
[22]
Brüggemann L, Dietrich P, Dreyer I, et al. (1999) Pronounced differences between the native K+ channels and KAT1 and KST1 alpha-subunit homomers of guard cells. Planta 207: 370–376. doi: 10.1007/s004250050494
[23]
Dreyer I, Michard E, Lacombe B, et al. (2001) A plant Shaker-like K+ channel switches between two distinct gating modes resulting in either inward-rectifying or "leak" current. FEBS Lett 505: 233–239. doi: 10.1016/S0014-5793(01)02832-0
[24]
Michard E, Lacombe B, Porée F, et al. (2005) A unique voltage sensor sensitizes the potassium channel AKT2 to phosphoregulation. J Gen Physiol 126: 605–617. doi: 10.1085/jgp.200509413
[25]
Michard E, Dreyer I, Lacombe B, et al. (2005) Inward rectification of the AKT2 channel abolished by voltage-dependent phosphorylation. Plant J 44: 783–797. doi: 10.1111/j.1365-313X.2005.02566.x
[26]
Xicluna J, Lacombe B, Dreyer I, et al. (2007) Increased functional diversity of plant K+ channels by preferential heteromerization of the Shaker-like subunits AKT2 and KAT2. J Biol Chem 282: 486–494. doi: 10.1074/jbc.M607607200
[27]
Geiger D, Becker D, Vosloh D, et al. (2009) Heteromeric AtKC1.AKT1 channels in Arabidopsis roots facilitate growth under K+-limiting conditions. J Biol Chem 284: 21288–21295.
[28]
Held K, Pascaud F, Eckert C, et al. (2011) Calcium-dependent modulation and plasma membrane targeting of the AKT2 potassium channel by the CBL4/CIPK6 calcium sensor/protein kinase complex. Cell Res 21: 1116–1130. doi: 10.1038/cr.2011.50
[29]
Garriga M, Raddatz N, Véry AA, et al. (2017) Cloning and functional characterization of HKT1 and AKT1 genes of Fragaria spp.-Relationship to plant response to salt stress. J Plant Physiol 210: 9–17.
[30]
Dreyer I, Müller-Röber B, Köhler B (2004) Voltage gated ion channels, Blatt MR, Annual Plant Reviews, Membrane Transport in Plants, Oxford: Blackwell Publishing, 150–192.
[31]
Eyring H (1935) The activated complex in chemical reactions. J Chem Phys 3: 107. doi: 10.1063/1.1749604
[32]
Dreyer I, Blatt MR (2009) What makes a gate? The ins and outs of Kv-like K+ channels in plants. Trends Plant Sci 14: 383–390.
[33]
Sharma T, Dreyer I, Riedelsberger J (2013) The role of K(+) channels in uptake and redistribution of potassium in the model plant Arabidopsis thaliana. Front Plant Sci 4: 224.
[34]
Sharma T, Dreyer I, Kochian L, et al. (2016) The ALMT family of organic acid transporters in plants and their involvement in detoxification and nutrient security. Front Plant Sci 7: 1488.
[35]
Loew LM, Schaff JC (2001) The Virtual Cell: a software environment for computational cell biology. Trends Biotechnol 19: 401–406. doi: 10.1016/S0167-7799(01)01740-1
[36]
Brüggemann L, Dietrich P, Becker D, et al. (1999) Channel-mediated high-affinity K+ uptake into guard cells from Arabidopsis. Proc Natl Acad Sci USA 96: 3298–3302. doi: 10.1073/pnas.96.6.3298
Table 5.
R-Bias∗100 and R-MSE∗100 (in parentheses) of the point estimates for model parameters based on 5000 replications under parameter settings II, III, and IV.
Figure 1. Comparison of the current through a homogenous pore and a structured K+ channel pore. (A) In a homogenous pore, the transmembrane potential difference drops linearly across the membrane (A, i) and the diffusion coefficient is constant over the entire distance but lower than in free solution (A, ii). (A, iii) Current voltage curves of a model cell with only homogeneous K+ selective ion channels and an internal potassium concentration of [K+]int = 120 mM calculated according to eqn. 2.1.6. Please note that all curves for [K+]ext < [K+]int are left-bended, which contradicts experimental findings for plant K+ uptake channels. (B) The pore of plant K+ uptake channels has an internal structure as resolved by homology modeling using the crystal structures of mammalian K+ channels as template [6,11,12,13,14,15]. Open K+ channels have in general an internal cavity that is connected to the inside of the cell. In this cavity the electric potential (B, i for x = 0…a) can be approximated to be identical to the potential inside the cell and the diffusion coefficient for K+ (Ds) is as in free solution (B, ii for x = 0…a). The narrow selectivity filter is the main obstacle for the passage of the ion. Here, the transmembrane potential difference drops across a short distance (B, i for x = a…b) and the diffusion coefficient (D) is smaller (B, ii for x = a…b). Beyond the selectivity filter (x = b…1) the conditions are as in free solution outside the cell. The conditions of plant K+ channels are well described with a = 0.75, b = 1, and D/Ds = 0.25. (B, iii) The current voltage curves were determined according to eqn. 2.1.9 and coincide very well with previously published experimental data
Figure 2. State model of an ion channel that can exist either in the open or in the closed conformation. Each conformation is characterized by a specific free energy (GO and GC, respectively). A conformational change implies a change of the energy status, whereby the energy barrier that has to overcome is determined by an instable intermediate conformation (GP). Mathematically, the transitions can be described by the rates a (activation; C→ O) and d (deactivation; O → C). Figure modified from Dreyer et al., 2004 [30]
Figure 3. Computational simulation of currents through voltage-gated ion channels. All three curves were generated with eqn. 2.2.9 using the indicated parameters. (A) Current voltage characteristic of inward-rectifying K+ channels. (B) Current voltage characteristic of outward-rectifying K+ channels. (C) Current voltage characteristic of organic acid channels of the QUAC-type. Vrev=RTzF⋅lncextcint
Figure 4. The plasma membrane H+-ATPase. (A) Mechanistic model illustrating the different steps of the pump cycle. Starting on top-left and then continuing clockwise: A proton from the internal medium binds to the protein. By using the energy from ATP-hydrolysis the protein gets phosphorylated. The phosphorylated protein with the proton bound is less stable and undergoes a conformational change that provides access of the proton to the external medium and that reduces the affinity of the proton to the protein. The proton dissociates and the phosphate gets cleaved off. The protein without phosphate and proton is more stable in the initial conformation. The cycle can start again. (B) Mathematical description of the pump current using eqn. 2.3.2. Please note that in this case the zero-current potential Vrev, at which Ipump(Vrev) = 0, is Vrev = -250 mV
Figure 5. In silico cell to study K+ uptake. The membrane of the in silico cell contains two transporter types: proton ATPase (H+ pump) and inward-rectifying K+ channel (Kin). Each transporter type is represented by one free parameter sK and Imax, respectively that describe their activity (expression level). The cell is characterized by surface and volume. The relevant concentrations for the model cell are the internal and external potassium and proton concentrations. To reflect the proton-buffer capacities in the cell and in the extracellular medium (usually buffered in uptake experiments by chemical buffers), the pH values were kept constant at pHint = 7.0 and pHext = 5.5
Figure 6. In silico cell to study K+ uptake. Simulation of [K]int accumulation with [K]ext = 10 mM starting from [K]int(0) = 1 mM. (A) Effect of a change in the density of Kin channels on the accumulation time course. A reference simulation (black curve) was repeated but with a ten-fold lower sK_rel (grey curve). (B) Effect of a change in the density of pumps on the accumulation time course. A reference simulation (black curve) was repeated but with a three-fold lower Imax_rel (grey curve). (C) Effect of a change in the cellular surface-to-volume ratio a on the accumulation time course. A reference simulation (black curve) was repeated but with a three-fold smaller value for a (grey curve)
Figure 7. Dual affinity behavior of Kin channels. (A) Rate of K+ absorption as a function of [K]ext measured at an early time point t1. (B) Eadie-Hofstee plot of the data from A. The data do not describe a straight line but a sum of at least two lines, one with a steep slope ("low affinity") and another with a moderate slope ("high affinity"). (C) Data from A displayed for two ranges (0-25 mM and 40 mM-25 mM) at different scales. The lines in A, and C represent best fits of the data with eqn. 3.2.2
Figure 8. Disappearance of dual affinity with increasing delay between the start of the experiment and the sampling time point. (A) Rate of K+ absorption as a function of [K]ext measured at a time point t2 > t1 displayed as in Figure 7A for two ranges. (B) Rate of K+ absorption as a function of [K]ext measured at a time point t3 > t2 displayed for two ranges. Lines represent best fits of the data with eqn. 3.2.2
Figure 9. Explanation of the disappearance of dual affinity with increasing delay between the start of the experiment and sampling time point. (Top panels) Time course of the internal K+ concentration ([K]int) for three different external K+ concentrations ([K]ext1 < [K]ext2 < [K]ext3). The flux is measured at the three different time points t1 (A), t2 (B), and t3 (C). The flux is the slope of the [K]int(t)-curve at the respective time point. The slopes of the three different curves are shown as dashed lines and cross-referenced by the different grey scales. (Bottom panels) Dependence of the slope (K+ flux) on the external K+ concentration at the different time points t1 (A), t2 (B), and t3 (C)