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Columnar structure of SV40 minichromosome

Genome Diversity Center, Institute of Evolution, University of Haifa, Mount Carmel, Haifa 3498838, Israel

Special Issues: Chromatin and Epigenetics

Like the sequence of the strongest 601 clone nucleosome of Lowary and Widom, the SV40 genome sequence contains tracks of YR dinucleotides separated by small integers of the 10.4n base series (10, 11, 21 and 30 bases). The tracks, however, substantially exceed the nucleosome DNA size and, thus, correspond to more extended structure - columnar chromatin. The micrococcal nuclease digests of the SV40 chromatin do not show uniquely positioned individual nucleosomes. This confirms the columnar structure of the minichromosome, as well as earlier electron microscopy studies.
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Keywords nucleosome; sequence periodicity; YR dinucleotides; YR tracks; stacked nucleosomes; rotational positioning; nucleosome sliding; nucleosome mapping

Citation: Edward N Trifonov. Columnar structure of SV40 minichromosome. AIMS Biophysics, 2015, 2(3): 274-283. doi: 10.3934/biophy.2015.3.274


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This article has been cited by

  • 1. Edward N. Trifonov, Nucleosome repeat lengths and columnar chromatin structure, Journal of Biomolecular Structure and Dynamics, 2015, 1, 10.1080/07391102.2015.1075158
  • 2. Vladimir B. Teif, Andrey G. Cherstvy, Chromatin and epigenetics: current biophysical views, AIMS Biophysics, 2016, 3, 1, 88, 10.3934/biophy.2016.1.88
  • 3. Edward N. Trifonov, Transcription factors operate TATA switches via rotational remodeling of local columnar chromatin structure, Journal of Biomolecular Structure and Dynamics, 2016, 1, 10.1080/07391102.2015.1134348

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Copyright Info: 2015, Edward N Trifonov, licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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