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A Cage-Based Training System for Non-Human Primates

1 Department of Cell Biology and Neuroscience, Montana State University (MSU), Bozeman, MT, USA
2 Gianforte School of Computing, Montana State University (MSU), Bozeman, MT, USA
3 School of Cognitive Sciences, Institute for Research in Fundamental Sciences, Tehran, Iran

Non-human primates (NHPs) are widely-used experimental models in neurophysiological studies. Training on cognitive tasks prior to collecting neurophysiological data is an inseparable part of much of the research conducted using NHPs. Any improvement in the training method that reduces stress to the animal, increases the speed of training or improves performance on the task is of great potential value. We have designed, built and successfully utilized a fully portable cage-mountable system to train rhesus monkeys (Macaca mulatta). The flexibility and portability of both the animal interface and the control unit of this system would allow it to be used for a large variety of behavioral paradigms. Aside from experimental use, our system could potentially be used as a source of animal enrichment. We present the behavioral data collected using this method to train a visual working memory and a change detection task. Utilizing the in-cage training system allows the animal greater control over when and how long it chooses to work, rather than imposing a training schedule based on the availability of the experimenter. Using this method the animal learned to perform both behavioral tasks in a short amount of time. In some cases the animal would use the training system without the need for any water restriction. In addition to allowing voluntary, self-paced engagement with the task, this method has the advantage of being less disruptive to the monkey's social interactions, and presumably eliminating some of the stress occasioned by relocating for chair training. Although this system has the potential to ease and expedite the behavioral training of NHPs on a variety of tasks, here we provide only a demonstration of our cage-based training system using one NHP.
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1. Rumbaugh DM, Hopkins WD, Washburn DA, et al. (1989) Lana chimpanzee learns to count by NUMATH: a summary of a videotaped experimental repxort. Psychol Rec 39: 459-470.    

2. Spinelli S, Pennanen L, Dettling AC, et al. (2004) Performance of the marmoset monkey on computerized tasks of attention and working memory. Cogn Brain Res 19: 123-137.    

3. Mandell DJ, Sackett GP (2009) Comparability of developmental cognitive assessments between standard and computer testing methods. Dev Psychobiol 51: 1-13.    

4. Truppa V, Garofoli D, Castorina G, et al. (2010) Identity concept learning in matching-to-sample tasks by tufted capuchin monkeys (Cebus apella). Anim Cogn 13: 835-848.

5. Vonk J, Torgerson-White L, McGuire M, et al. (2014) Quantity estimation and comparison in western lowland gorillas (Gorilla gorilla gorilla). Anim Cogn 17: 755-765.    

6. Allritz M, Call J, Borkenau P (2016) How chimpanzees (Pan troglodytes) perform in a modified emotional Stroop task. Anim Cogn 19: 435-449.    

7. Wagner KE, Hopper LM, Ross SR (2016) Asymmetries in the production of self-directed behavior by chimpanzees and gorillas during a computerized cognitive test. Anim Cogn 19: 343-350.    

8. Andrews MW, Rosenblum LA (1993) Live-Social-Video Reward Maintains Joystick Task Performance in Bonnet Macaques. Percept Mot Skills 77: 755-763.    

9. Andrews MW (1993) Video-task paradigm extended to Saimiri. Percept Mot Skills 76: 183–191.    

10. Andrews MW, Rosenblum LA (1994) Automated recording of individual performance and hand preference during joystick-task acquisition in group-living bonnet macaques (Macaca radiata). J. Comp Psychol 108: 358-362.

11. Andrews MW (1994) Effective use of a joystick by an infant monkey. Am J Primatol 32: 141-144.

12. Leighty KA, Fragaszy DM (2003) Joystick acquisition in tufted capuchins (Cebus apella). Anim Cogn 6: 141-148.    

13. Scott L, Pearce P, Fairhall S, et al. (2010) Training Nonhuman Primates to Cooperate With Scientific Procedures in Applied Biomedical Research. J Appl Anim Welf Sci 6: 199-207.

14. Judge PG, Kurdziel LB, Wright RM, et al. (2012) Picture recognition of food by macaques (Macaca silenus). Anim Cogn 15: 313-325.

15. Joly M, Ammersdörfer S, Schmidtke D, et al. (2014) Touchscreen-Based Cognitive Tasks Reveal Age-Related Impairment in a Primate Aging Model, the Grey Mouse Lemur (Microcebus murinus). PLoS One 9: e109393.    

16. Schmitt V, Federspiel I, Eckert J, et al. (2016) Do monkeys compare themselves to others? Anim Cogn 19: 417-428.    

17. Jacobsen ME, Barros M, Maior RS (2017) MK-801 reduces sensitivity to Müller-Lyer's illusion in capuchin monkeys. Behav Brain Res 316: 54-58.

18. Washburn DA, Hopkins WD, Rumbaugh DM (1989) Video-task assessment of learning and memory in macaques (Macaca mulatta): effects of stimulus movement on performance. J Exp Psychol Anim Behav Process 15: 393-400.    

19. Richardson WK, Washburn DA, Hopkins WD, et al. (1990) The NASA/LRC computerized test system. Behav Res Methods Instruments & amp Comput 22: 127-131.

20. Washburn DA, Hopkins WD, Rumbaugh DM (1991) Perceived control in rhesus monkeys (Macaca mulatta): enhanced video-task performance. J Exp Psychol Anim Behav Process 17: 123-129.

21. Washburn DA, Rumbaugh DM (1992) Investigations of rhesus monkey video-task performance: evidence for enrichment. Contemp Top Lab Anim Sci 31: 6-10.

22. Washburn DA, Hopkins WD (1994) Videotape-versus pellet-reward preferences in joystick tasks by macaques. Percept Mot Skills 78: 48-50.    

23. Weed MR, Taffe M A, Polis I, et al. (1999) Performance norms for a rhesus monkey neuropsychological testing battery: acquisition and long-term performance. Brain Res Cogn Brain Res 8: 185-201.    

24. Fagot J, Paleressompoulle D (2009) Automatic testing of cognitive performance in baboons maintained in social groups. Behav Res Methods 41: 396-404.    

25. Watson KK, Ghodasra JH, Furlong MA, et al. (2012) Visual preferences for sex and status in female rhesus macaques. Anim Cogn 15: 401-407.    

26. Gazes RP, Brown EK, Basile BM, et al. (2013) Automated cognitive testing of monkeys in social groups yields results comparable to individual laboratory-based testing. Anim Cogn 16: 445-458.    

27. Hutsell BA, Banks ML (2015) Effects of environmental and pharmacological manipulations on a novel delayed nonmatching-to-sample 'working memory' procedure in unrestrained rhesus monkeys. J Neurosci Methods 251: 62-71.    

28. Calapai A, Berger M, Niessing M, et al. (2017) A cage-based training, cognitive testing and enrichment system optimized for rhesus macaques in neuroscience research. Behav Res Methods 49: 35-45.    

29. Sorwell KG, Renner L, Weiss AR, et al. (2017) Cognition in aged rhesus monkeys: effect of DHEA and correlation with steroidogenic gene expression. Genes Brain Behav 16: 361-368.    

30. Fiuzat EC, Rhodes SEV, Murray EA (2017) The Role of Orbitofrontal–Amygdala Interactions in Updating Action–Outcome Valuations in Macaques. J Neurosci 10: 1839-1816.

31. Fagot J, Parron C (2010) Relational Matching in Baboons (Papio papio) With Reduced Grouping Requirements. J Exp Psychol Anim Behav Process 36: 184-193.    

32. Fagot J, Bonté E (2010) Automated testing of cognitive performance in monkeys: Use of a battery of computerized test systems by a troop of semi-free-ranging baboons (Papio papio). Behav Res Methods 42: 507-516.    

33. Fagot J, Gullstrand J, Kemp C, et al. (2014) Effects of freely accessible computerized test systems on the spontaneous behaviors and stress level of Guinea baboons (Papio papio). Am J Primatol 76: 56-64.

34. Washburn DA, Rumbaugh DM (1992) Testing primates with joystick-based automated apparatus: Lessons from the Language Research Center's Computerized Test System. Behav Res Methods Instruments Comput 24: 157-164.

35. Washburn DA, Harper S, Rumbaugh DM (1994) Computer-task testing of rhesus monkeys (Macaca mulatta) in the social milieu. Primates 35: 343-351.    

36. Bennett AJ, Perkins CM, Tenpas PD, et al. (2016) Moving evidence into practice: cost analysis and assessment of macaques' sustained behavioral engagement with videogames and foraging devices. Am J Primatol 78: 1250-1264.    

37. Srihasam K, Vincent JL, Livingstone MS (2014) Novel domain formation reveals proto-architecture in inferotemporal cortex. Nat Neurosci 12: 1776-1783.

38. Bangasser DA, Shors TJ (2010) Critical brain circuits at the intersection between stress and learning. Neurosci Biobehav Rev 34: 1223-1233.    

39. Slater H, Milne AE, Wilson B, et al. (2016) Individually customisable non-invasive head immobilisation system for non-human primates with an option for voluntary engagement. J Neurosci Methods 269: 46-60.    

40. Howell LL, Hoffman JM, Votaw JR, et al. (2001) An apparatus and behavioral training protocol to conduct positron emission tomography (PET) neuroimaging in conscious rhesus monkeys. J Neurosci Methods 106: 161-169.    

41. Drucker CB, Carlson ML, Toda K, et al. (2015) Non-invasive primate head restraint using thermoplastic masks. J Neurosci Methods 253: 90-100.    

42. Machado CJ, Nelson EE (2011) Eye-tracking with nonhuman primates is now more accessible than ever before. Am J Primatol 73: 562-569.    

43. Fairhall SJ, Dickson CA, Scott L, et al. (2006) A non-invasive method for studying an index of pupil diameter and visual performance in the rhesus monkey. J Med Primatol 35: 67-77.

44. Kiorpes L, Price T, Hall-Haro C, et al. (2012) Development of sensitivity to global form and motion in macaque monkeys (Macaca nemestrina). Vision Res 63: 34-42.    

Copyright Info: © 2017, Behrad Noudoost, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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