Single cell adhesion strength assessed with variable-angle total internal reflection fluorescence microscopy

Marcelina Cardoso Dos Santos; Cyrille Vézy; Hamid Morjani; Rodolphe Jaffol; Marcelina Cardoso Dos Santos; Cyrille Vézy; Hamid Morjani; Rodolphe Jaffol

doi:10.3934/biophy.2017.3.438

AIMS Biophysics

2017, Volume 4, Issue 3: 438-450. doi: 10.3934/biophy.2017.3.438

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Single cell adhesion strength assessed with variable-angle total internal reflection fluorescence microscopy

1.
Laboratoire de Nanotechnologie et d’Instrumentation Optique, Institut Charles Delaunay, UMR CNRS 6281, Université de Technologie de Troyes, 12 rue Marie Curie, CS 42060, 10 004 Troyes cedex, France
2.
NanoBioPhotonics group, Institute for Integrative Biology of the Cell, Université Paris-Saclay, CNRS, CEA, Bâtiment 430, 91405 Orsay cedex, France
3.
Laboratoire Matrice Extracellulaire et Dynamique Cellulaire, UMR CNRS 7369, Universit´e de Reims Champagne-Ardennes, 51 rue Cognacq-Jay, 51096 Reims Cedex, France

Received: 06 April 2017 Accepted: 18 June 2017 Published: 28 June 2017

We propose a new strategy to evaluate adhesion strength at the single cell level. This approach involves variable-angle total internal reflection fluorescence microscopy to monitor in real time the topography of cell membranes, i.e. a map of the membrane/substrate separation distance. According to the Boltzmann distribution, both potential energy profile and dissociation energy related to the interactions between the cell membrane and the substrate were determined from the membrane topography. We have highlighted on glass substrates coated with poly-L-lysine and fibronectin, that the dissociation energy is a reliable parameter to quantify the adhesion strength of MDA-MB-231 motile cells.
- biophotonics,
- total internal reflection fluorescence microscopy,
- super-resolution microscopy,
- cell adhesion,
- cell membrane-substrate interactions
Citation: Marcelina Cardoso Dos Santos, Cyrille Vézy, Hamid Morjani, Rodolphe Jaffol. Single cell adhesion strength assessed with variable-angle total internal reflection fluorescence microscopy[J]. AIMS Biophysics, 2017, 4(3): 438-450. doi: 10.3934/biophy.2017.3.438

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Abstract

We propose a new strategy to evaluate adhesion strength at the single cell level. This approach involves variable-angle total internal reflection fluorescence microscopy to monitor in real time the topography of cell membranes, i.e. a map of the membrane/substrate separation distance. According to the Boltzmann distribution, both potential energy profile and dissociation energy related to the interactions between the cell membrane and the substrate were determined from the membrane topography. We have highlighted on glass substrates coated with poly-L-lysine and fibronectin, that the dissociation energy is a reliable parameter to quantify the adhesion strength of MDA-MB-231 motile cells.

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