Transcriptional activity of cytokines induced by oral administration of <em>Lactobacillus casei</em> and <em>Lactobacillus acidophilus</em> in experimental model of arthritis

Sarika Amdekar; Avnish Kumar; Vinod Singh; Sarika Amdekar; Avnish Kumar; Vinod Singh

doi:10.3934/molsci.2017.2.164

AIMS Molecular Science

2017, Volume 4, Issue 2: 164-174. doi: 10.3934/molsci.2017.2.164

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Transcriptional activity of cytokines induced by oral administration of Lactobacillus casei and Lactobacillus acidophilus in experimental model of arthritis

1.
Department of Microbiology, Barkatullah University, Hoshangabad Road, Habibganj, Bhopal, Madhya Pradesh 462026, India
2.
Department of Biotechnology, Dr. B. R. Ambedkar University, Khandari Campus, Agra, Uttar Pradesh 282004, India

Received: 22 December 2016 Accepted: 27 April 2017 Published: 12 May 2017

Lactobacillus casei and Lactobacillus acidophilus have been evaluated for the inhibitory activity of pro-inflammatory cytokine gene expressions. Collagen induced arthritic model was used. L. casei and L. acidophilus of 2 × 10⁸ CFU/mL was orally administered for 28 days. IL-6, IL-1β, TNF, IL-4 and IL-10 in serum were estimated using ELISA. mRNA gene expressions of the same cytokines in knee joints were also analyzed using RT PCR. Paw thickness was measured at the end of each week. Lactobacillus treatment statistically increased IL-4 and IL-10 levels while decreased IL-6, IL-1β and TNF. mRNA expression of IL-6, IL-1β and TNF gene were highly stimulated in control group animals joint samples. Lactobacillus and drug treatment were restored to normal in other groups. mRNA expression of IL-10 and IL-4 were highly expressed in Lactobacillus administered groups. Lactobacillus was effective in alleviating the inflammatory responses and thus arthritis symptoms. Lactobacillus could be considered as a dietary agent against arthritis.
- pro-inflammatory cytokines,
- RT PCR,
- Lactobacillus,
- probiotics
Citation: Sarika Amdekar, Avnish Kumar, Vinod Singh. Transcriptional activity of cytokines induced by oral administration of Lactobacillus casei and Lactobacillus acidophilus in experimental model of arthritis[J]. AIMS Molecular Science, 2017, 4(2): 164-174. doi: 10.3934/molsci.2017.2.164

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Abstract

Lactobacillus casei and Lactobacillus acidophilus have been evaluated for the inhibitory activity of pro-inflammatory cytokine gene expressions. Collagen induced arthritic model was used. L. casei and L. acidophilus of 2 × 10⁸ CFU/mL was orally administered for 28 days. IL-6, IL-1β, TNF, IL-4 and IL-10 in serum were estimated using ELISA. mRNA gene expressions of the same cytokines in knee joints were also analyzed using RT PCR. Paw thickness was measured at the end of each week. Lactobacillus treatment statistically increased IL-4 and IL-10 levels while decreased IL-6, IL-1β and TNF. mRNA expression of IL-6, IL-1β and TNF gene were highly stimulated in control group animals joint samples. Lactobacillus and drug treatment were restored to normal in other groups. mRNA expression of IL-10 and IL-4 were highly expressed in Lactobacillus administered groups. Lactobacillus was effective in alleviating the inflammatory responses and thus arthritis symptoms. Lactobacillus could be considered as a dietary agent against arthritis.

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