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AIMS Microbiology

2017, Volume 3, Issue 2: 315-322. doi: 10.3934/microbiol.2017.2.315
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Research article

Modeling growth of Alicyclobacillus acidoterrestris DSM 3922 type strain vegetative cells in the apple juice with nisin and lysozyme

  • Department of Food Engineering, Izmir Institute of Technology, Urla, 35430, Izmir, Turkey
  • Received: 05 March 2017 Accepted: 27 April 2017 Published: 05 May 2017

  • In the present study, the effect of storage temperature on A. acidoterrestris DSM 3922 cells (105 CFU/mL) was examined during growth in reconstituted apple juice (pH 3.8, °Brix 11.3) containing nisin (0–100 IU/mL) and lysozyme (0–100 mg/L). The growth curves were obtained at three temperatures of 27, 35 and 43 °C using absorbance data (OD600 nm). Based on the results, the minimal inhibitory concentrations (MICs) of nisin were found as 10 IU/mL at all tested temperatures. On the other hand, increasing the temperature decreased the amount of lysozyme for growth inhibition. The MICs of lysozyme were found as 10, 2.5 and 1.25 mg/L at 27, 35 and 43 °C, respectively. At selected non-inhibitory doses, nisin (1.25–5 IU/mL) and lysozyme (0.3–2.5 mg/L) prolonged the lag time compared to the controls at the corresponding temperatures. In addition, there was a strong linear relationship between the lag time and lysozyme concentrations at 27 and 35 °C (R2 > 0.98). The results of this study demonstrated that both nisin and lysozyme could be used to inhibit the growth of A. acidoterrestris cells in the apple juice. The results also indicated that the growth parameters were variable depending on the storage temperature and the type of the antimicrobial agent used in the apple juice.

    Citation: Celenk Molva, Ayse Handan Baysal. Modeling growth of Alicyclobacillus acidoterrestris DSM 3922 type strain vegetative cells in the apple juice with nisin and lysozyme[J]. AIMS Microbiology, 2017, 3(2): 315-322. doi: 10.3934/microbiol.2017.2.315

    Related Papers:

  • In the present study, the effect of storage temperature on A. acidoterrestris DSM 3922 cells (105 CFU/mL) was examined during growth in reconstituted apple juice (pH 3.8, °Brix 11.3) containing nisin (0–100 IU/mL) and lysozyme (0–100 mg/L). The growth curves were obtained at three temperatures of 27, 35 and 43 °C using absorbance data (OD600 nm). Based on the results, the minimal inhibitory concentrations (MICs) of nisin were found as 10 IU/mL at all tested temperatures. On the other hand, increasing the temperature decreased the amount of lysozyme for growth inhibition. The MICs of lysozyme were found as 10, 2.5 and 1.25 mg/L at 27, 35 and 43 °C, respectively. At selected non-inhibitory doses, nisin (1.25–5 IU/mL) and lysozyme (0.3–2.5 mg/L) prolonged the lag time compared to the controls at the corresponding temperatures. In addition, there was a strong linear relationship between the lag time and lysozyme concentrations at 27 and 35 °C (R2 > 0.98). The results of this study demonstrated that both nisin and lysozyme could be used to inhibit the growth of A. acidoterrestris cells in the apple juice. The results also indicated that the growth parameters were variable depending on the storage temperature and the type of the antimicrobial agent used in the apple juice.


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    [1] Silva FVM, Gibbs P (2001) Alicyclobacillus acidoterrestris spores in fruit products and design of pasteurization processes. Trends Food Sci Technol 12: 68–74. doi: 10.1016/S0924-2244(01)00070-X
    [2] Hippchen B, Roll A, Poralla K (1981) Occurrence in soil of thermo-acidophilic bacilli possessing ω-cyclohexane fatty acids and hopanoids. Arch Microbiol 129: 53–55. doi: 10.1007/BF00417180
    [3] Walker M, Phillips CA (2008) Alicyclobacillus acidoterrestris: An increasing threat to the fruit juice industry? Int J Food Sci Technol 43: 250–260.
    [4] Walker M, Phillips CA (2005) The effect of intermittent shaking, headspace and temperature on the growth of Alicyclobacillus acidoterrestris in stored apple juice. Int J Food Sci Technol 40: 557–562. doi: 10.1111/j.1365-2621.2005.00960.x
    [5] Huertas JP, Esteban MD, Antolinos V, et al. (2015) Combined effect of natural antimicrobials and thermal treatments on Alicyclobacillus acidoterrestris spores. Food Control 35: 73–78.
    [6] Aguirre JS, de Fernando GG, Hierro E, et al. (2015) Estimation of the growth parameters of Bacillus cereus spores as affected by pulsed light treatment. Int J Food Microbiol 202: 20–26. doi: 10.1016/j.ijfoodmicro.2015.02.020
    [7] Shin JM, Gwak JW, Kamarajan P, et al. (2016) Biomedical applications of nisin. J Appl Microbiol 120: 1449–1465. doi: 10.1111/jam.13033
    [8] Antolinos V, Muňoz M, Ros-Chumillas M, et al. (2011) Combined effect of lysozyme and nisin at different incubation temperature and mild heat treatment on the probability of time to growth of Bacillus cereus. Food Microbiol 28: 305–310. doi: 10.1016/j.fm.2010.07.021
    [9] Dussault D, Vu KD, Lacroix M (2016) Development of a model describing the inhibitory effect of selected preservatives on the growth of Listeria monocytogenes in a meat model system. Food Microbiol 53: 115–121. doi: 10.1016/j.fm.2015.09.011
    [10] Komitopoulou E, Boziaris IS, Davies EA, et al. (1999) Alicyclobacillus acidoterrestris in fruit juices and its control by nisin. Int J Food Sci Technol 34: 81–85. doi: 10.1046/j.1365-2621.1999.00243.x
    [11] Yamazaki K, Murakami M, Kawai Y, et al. (2000) Use of nisin for inhibition of Alicyclobacillus acidoterrestris in acidic drinks. Food Microbiol 17: 315–320. doi: 10.1006/fmic.1999.0309
    [12] Penã WE, De Massaguer PR (2006) Microbial modeling of Alicyclobacillus acidoterrestris CRA 7152 growth in orange juice with nisin added. J Food Prot 69: 1904–1912. doi: 10.4315/0362-028X-69.8.1904
    [13] Buonocore GG, Sinigaglia M, Corbo MR, et al. (2004) Antimicrobial release systems from highly swellable polymers. J Food Prot 67: 1190–1194. doi: 10.4315/0362-028X-67.6.1190
    [14] Bevilaqua A, Sinigaglia M, Corbo MR (2008) Alicyclobacillus acidoterrestris: New methods for inhibiting spore germination. Int J Food Microbiol 125: 103–110. doi: 10.1016/j.ijfoodmicro.2008.02.030
    [15] Silvetti T, Morandi S, Hintersteiner M, et al. (2017) Use of hen egg white lysozyme in the food industry, In: Hester P, Editor, Innovations and Strategies for Improvements, Academic Press, 233–242.
    [16] Zhao J, Gao J, Chen F, et al. (2014) Modeling and predicting the effect of temperature on the growth on Proteus mirabilis in chicken. J Microbiol Methods 99: 38–43. doi: 10.1016/j.mimet.2014.01.016
    [17] Molva C, Baysal AH (2014) Effect of sporulation medium on wet-heat resistance and structure of Alicyclobacillus acidoterrestris DSM 3922-type strain spores and modeling of the inactivation kinetics in apple juice. Int J Food Microbiol 189: 82–88. doi: 10.1016/j.ijfoodmicro.2014.07.033
    [18] Baranyi J, Roberts TA (1994) A dynamic approach to predicting microbial growth in food. Int J Food Microbiol 23: 277–294. doi: 10.1016/0168-1605(94)90157-0
    [19] Ray B, Daeschel M (2000) Nisin of Lactococcus lactis as a biopreservative, In: Food biopreservatives of microbial origin, CRC Press Inc., FL.
    [20] Delves BJ (1999) Nisin and its uses as a food preservative. Int J Dairy Technol 43: 100–112.
    [21] Tianli Y, Jiangbo Z, Yahong Y (2014) Spoilage by Alicyclobacillus bacteria in juice and beverage products: chemical, physical, and combined control methods. Compr Rev Food Sci Food Safe 13: 771–797. doi: 10.1111/1541-4337.12093
    [22] Ibrahim HR, Higashiguchi S, Koketsu M, et al. (1996) Partially unfolded lysozyme at neutral pH agglutinates and kills Gram-negative and Gram-positive bacteria through membrane damage mechanism. J Agr Food Chem 44: 3799–3806. doi: 10.1021/jf960133x
    [23] Bevilaqua A, Corbo MR, Buonocore GG, et al. (2007) Antimicrobial effectiveness of lysozyme against Alicyclobacillus acidoterrestris. Adv Food Sci 29: 47–52.
    [24] Esteban MD, Palop A (2011) Nisin, carvacrol and their combinations against the growth of heat-treated Listeria monocytogenes cells. Food Technol Biotechnol 49: 89–95.
    [25] Ross T (1996) Indices for performance evaluation of predictive models in food microbiology. J App Bacteriol 81: 501–508.
    [26] Altieri C, Bevilacqua A, Cardillo D, et al. (2006) Modeling Alicyclobacillus acidoterrestris under monolaurin action. Adv Food Sci 28: 94–99.
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