Effect of curcumin nano-micelle on of 1,2, dimethylhydrazine-induced rat colon carcinogenesis in comparison with 5FU

Hamidreza Mohammadi; Mohammad Shokrzadeh; Majid Darvish; Ayat Dashti; Majid Jafari Sabet; Saeed Kavoosian; Ramin Ataee; Hamidreza Mohammadi; Mohammad Shokrzadeh; Majid Darvish; Ayat Dashti; Majid Jafari Sabet; Saeed Kavoosian; Ramin Ataee

doi:10.3934/medsci.2020013

AIMS Medical Science

2020, Volume 7, Issue 3: 223-233. doi: 10.3934/medsci.2020013

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Research article

Effect of curcumin nano-micelle on of 1,2, dimethylhydrazine-induced rat colon carcinogenesis in comparison with 5FU

1.
Pharmaceutical Sciences Research center, Mazandaran University of Medical Sciences, Sari Iran
2.
Department of Pharmacology, Iran University of Medical Sciences, Tehran Iran
3.
Amol Branch, Institute Pasteur of Iran, Amol, Iran

Received: 21 June 2020 Accepted: 02 September 2020 Published: 15 September 2020

Objective Curcumin, a natural polyphenol that has anti-oxidative, anti-inflammatory and anti-cancer properties. Therapeutic potential of curcumin is substantially limited by the rather low water solubility, rapid metabolism and low bioavailability. Therefore it is necessary for its' suitable carriers. In the present study, it was aimed to assess of polymeric micelles of curcumin on experimental model of colon cancer in Rat. Method Pathogen-free male Sprague–Dawley rats divided into 4 groups. Animals from group A, B, C received DMH intraperitoneal injection once a week at a dose of 20 mg/kg body weight in 0.9% normal saline. Group A, as DMH control. B, C received Nano-micelle curcumin and 5-FU respectively 4 weeks before first carcinogen challenge and continued until week 30 of experimentation. After 4 weeks all animals were killed and their colon removed and investigated for morphological and immunohistochemistry studies of apoptosis and expression of Bcl2, BAX and Caspase9. Results It was observed significant morphological changes in Nano-curcumin groups compared with DMH group but for expression of apoptotic factors, although decrease in Bcl2 expression and increase in BAX and caspase9 observed in Nano-micelle group but these changes were not significant compared with DMH group (P > 0.05). Conclusion Results of this study indicate that Nano-micelle of curcumin has some preventive effect in experimental model of colon cancer in vivo but has not induced complete apoptotic effect and more studies that are complete are needed.
- nano-micelle curcumin,
- colon cancer,
- in vivo study,
- dimethyl-hydrazine,
- apoptosis
Citation: Hamidreza Mohammadi, Mohammad Shokrzadeh, Majid Darvish, Ayat Dashti, Majid Jafari Sabet, Saeed Kavoosian, Ramin Ataee. Effect of curcumin nano-micelle on of 1,2, dimethylhydrazine-induced rat colon carcinogenesis in comparison with 5FU[J]. AIMS Medical Science, 2020, 7(3): 223-233. doi: 10.3934/medsci.2020013

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Abstract

Objective Curcumin, a natural polyphenol that has anti-oxidative, anti-inflammatory and anti-cancer properties. Therapeutic potential of curcumin is substantially limited by the rather low water solubility, rapid metabolism and low bioavailability. Therefore it is necessary for its' suitable carriers. In the present study, it was aimed to assess of polymeric micelles of curcumin on experimental model of colon cancer in Rat. Method Pathogen-free male Sprague–Dawley rats divided into 4 groups. Animals from group A, B, C received DMH intraperitoneal injection once a week at a dose of 20 mg/kg body weight in 0.9% normal saline. Group A, as DMH control. B, C received Nano-micelle curcumin and 5-FU respectively 4 weeks before first carcinogen challenge and continued until week 30 of experimentation. After 4 weeks all animals were killed and their colon removed and investigated for morphological and immunohistochemistry studies of apoptosis and expression of Bcl2, BAX and Caspase9. Results It was observed significant morphological changes in Nano-curcumin groups compared with DMH group but for expression of apoptotic factors, although decrease in Bcl2 expression and increase in BAX and caspase9 observed in Nano-micelle group but these changes were not significant compared with DMH group (P > 0.05). Conclusion Results of this study indicate that Nano-micelle of curcumin has some preventive effect in experimental model of colon cancer in vivo but has not induced complete apoptotic effect and more studies that are complete are needed.

Acknowledgments

Authors appreciate all nice efforts of pharmacology and toxicology lab staff of pharmacy school of Mazandaran university of medical sciences, Sari, Iran for all nice collaborations.

Funding sources

All procedures of this project were under financial support and supervision of deputy of research of Mazandarn university of medical sciences, Sari, Iran, with project code of 2824.

Author contributions

Majid Darvish who was student of pharmacy contributed in all experimental and practical steps of the article and accumulating of DATA, Hamidreza Mohammadi as supervision of project and leader of Nano micelle assay and formulation, Mohammad Shokrzadeh as supervisor and leader of dose determining and toxicology assays, Ayat Dashti contributed in all experimental and practical steps of the articl Majid Jafari-Sabet as pharmacologist contributed as consultant of pharmacological aspects of the study. Ramin Ataee as main supervisor and designer of the study and contributed also in IHC procedures and its interpretation.

Conflict of interest

Authors disclose that there is not any possible conflicts of interest for all forms of support and financial involvement (e.g. employment, consultancies, honoraria, stock ownership and options, expert testimony, grants or patents received or pending, royalties) Also there was not any nonfinancial relationships (personal, political, or professional) that may potentially influence the writing of the manuscript.

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