Research article Special Issues

The refractive index of human blood measured at the visible spectral region by single-fiber reflectance spectroscopy

  • Received: 17 October 2020 Accepted: 14 December 2020 Published: 17 December 2020
  • The refractive index is an essential biophysical parameter used in many diagnostic and therapeutic biomedical applications. In the present study, the refractive index of control and anemic blood was measured using the total internal reflection fiber optics technique. For control, the refractive index measured by the indicated method was significantly higher than anemic blood over the wavelengths in the visible spectral region used in this study. Strong linear correlations between refractive index and hemoglobin concentration were obtained for control and anemic blood. These findings could enhance the use of the refractive index in many applications of blood analysis and hematology.

    Citation: Mohamed A. Elblbesy. The refractive index of human blood measured at the visible spectral region by single-fiber reflectance spectroscopy[J]. AIMS Biophysics, 2021, 8(1): 57-65. doi: 10.3934/biophy.2021004

    Related Papers:

  • The refractive index is an essential biophysical parameter used in many diagnostic and therapeutic biomedical applications. In the present study, the refractive index of control and anemic blood was measured using the total internal reflection fiber optics technique. For control, the refractive index measured by the indicated method was significantly higher than anemic blood over the wavelengths in the visible spectral region used in this study. Strong linear correlations between refractive index and hemoglobin concentration were obtained for control and anemic blood. These findings could enhance the use of the refractive index in many applications of blood analysis and hematology.


    加载中


    [1] Tuchin VV (2007) Optical biomedical diagnostics. Moscow: Izdatelstvo Fizikomatematicheskoy Literaturi 1: 560.
    [2] Jacques SL (2013) Optical properties of biological tissues: a review. Phys Med Biol 58: R37. doi: 10.1088/0031-9155/58/11/R37
    [3] Bashkatov AN, Genina EA, Tuchin VV (2011) Optical properties of skin, subcutaneous, and muscle tissues: a review. J Innov Opt Heal Sci 4: 9-38. doi: 10.1142/S1793545811001319
    [4] Tuchin VV, Maksimova IL, Zimnyakov DA, et al. (1997) Light propagation in tissues with controlled optical properties. J Biomed Opt 2: 401-418. doi: 10.1117/12.281502
    [5] Wray JH, Neu JT (1969) Refractive index of several glasses as a function of wavelength and temperature. JOSA 59: 774-776. doi: 10.1364/JOSA.59.000774
    [6] Ding H, Lu JQ, Wooden WA, et al. (2006) Refractive indices of human skin tissues at eight wavelengths and estimated dispersion relations between 300 and 1600 nm. Phys Med Biol 51: 1479. doi: 10.1088/0031-9155/51/6/008
    [7] Räty J, Pääkkönen P, Peiponen KE (2012) Assessment of wavelength dependent complex refractive index of strongly light absorbing liquids. Opt Express 20: 2835-2843. doi: 10.1364/OE.20.002835
    [8] Serbetçi Z, El-Nasser HM, Yakuphanoglu F (2012) Photoluminescence and refractive index dispersion properties of ZnO nanofibers grown by sol–gel method. Spectrochim Acta A 86: 405-409. doi: 10.1016/j.saa.2011.10.058
    [9] Kim SH, Lee SH, Lim JI, et al. (2010) Absolute refractive index measurement method over a broad wavelength region based on white-light interferometry. Appl Optics 49: 910-914. doi: 10.1364/AO.49.000910
    [10] Shi W, Fang C, Yin X, et al. (1999) Refractive index dispersion measurement on nonlinear optical polymer using V-prism refractometer. Opt Laser Eng 32: 41-47. doi: 10.1016/S0143-8166(99)00047-0
    [11] Tuchin VV (2015) Tissue optics. Society of Photo-Optical Instrumentation Engineers (SPIE) .
    [12] Meinke MC, Müller GJ, Helfmann J, et al. (2007) Optical properties of platelets and blood plasma and their influence on the optical behavior of whole blood in the visible to near infrared wavelength range. J Biomed Opt 12: 014024. doi: 10.1117/1.2435177
    [13] Tuchin VV (2002)  Handbook of optical biomedical diagnostics SPIE-The International Society for Optical Engineering.
    [14] Wang J, Deng Z, Wang X, et al. (2015) Measurement of the refractive index of hemoglobin solutions for a continuous spectral region. Biomed Opt Express 6: 2536-2541. doi: 10.1364/BOE.6.002536
    [15] Bosschaart N, Edelman GJ, Aalders MCG, et al. (2014) A literature review and novel theoretical approach on the optical properties of whole blood. Laser Med Sci 29: 453-479. doi: 10.1007/s10103-013-1446-7
    [16] Zhernovaya O, Sydoruk O, Tuchin V, et al. (2011) The refractive index of human hemoglobin in the visible range. Phys Med Biol 56: 4013. doi: 10.1088/0031-9155/56/13/017
    [17] Rowe DJ, Smith D, Wilkinson JS (2017) Complex refractive index spectra of whole blood and aqueous solutions of anticoagulants, analgesics and buffers in the mid-infrared. Sci Rep 7: 1-9. doi: 10.1038/s41598-016-0028-x
    [18] Barer R (1957) Refractometry and interferometry of living cells. JOSA 47: 545-556. doi: 10.1364/JOSA.47.000545
    [19] Khlebtsov NG, Maksimova IL, Tuchin VV, et al. (2002)  Introduction to Light Scattering by Biological Objects. Part 1. Extinction and Scattering of Light in Disperse Systems Society of Photo-Optical Instrumentation Engineers.
    [20] Lazareva EN, Tuchin VV (2018) Blood refractive index modelling in the visible and near infrared spectral regions. J Biomed Photonics Eng 4. doi: 10.18287/JBPE18.04.010503
    [21] Ramakrishnan S Textbook of Medical Biochemistry (2004) .
    [22] Taparia N, Platten KC, Anderson KB, et al. (2017) A microfluidic approach for hemoglobin detection in whole blood. AIP Adv 7: 105102. doi: 10.1063/1.4997185
    [23] Serebrennikova YM, Huffman DE, Garcia-Rubio LH (2015) Characterization of red blood cells with multiwavelength transmission spectroscopy. BioMed Res Int .
    [24] Ergül Ö, Arslan-Ergül A, Gürel L (2010) Computational study of scattering from healthy and diseased red blood cells. J Biomed Opt 15: 045004. doi: 10.1117/1.3467493
    [25] Zhang XU, Faber DJ, Post AL, et al. (2019) Refractive index measurement using single fiber reflectance spectroscopy. J Biophotonics 12: e201900019.
    [26] Zhang XU, Post AL, Faber DJ, et al. (2017) Single fiber reflectance spectroscopy calibration. J Biomed Opt 22: 100502.
    [27] Sardar DK, Levy LB (1998) Optical properties of whole blood. Laser Med Sci 13: 106-111. doi: 10.1007/s101030050062
    [28] Cheng S, Shen HY, Zhang G, et al. (2002) Measurement of the refractive index of biotissue at four laser wavelengths, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment. Int Society Opt Photonics 4916: 172-176.
    [29] Bolin FP, Preuss LE, Taylor RC, et al. (1989) Refractive index of some mammalian tissues using a fiber optic cladding method. Appl Optics 28: 2297-2303. doi: 10.1364/AO.28.002297
    [30] Liu S, Deng Z, Li J, et al. (2019) Measurement of the refractive index of whole blood and its components for a continuous spectral region. J Biomed Opt 24: 035003.
    [31] Liu PY, Chin LK, Ser W, et al. (2016) Cell refractive index for cell biology and disease diagnosis: past, present and future. Lab Chip 16: 634-644. doi: 10.1039/C5LC01445J
    [32] Faber DJ, Aalders MCG, Mik EG, et al. (2004) Oxygen saturation-dependent absorption and scattering of blood. Phys Rev Lett 93: 028102. doi: 10.1103/PhysRevLett.93.028102
    [33] Jin YL, Chen JY, Xu L, et al. (2006) Refractive index measurement for biomaterial samples by total internal reflection. Phys Med Biol 51: N371-N379. doi: 10.1088/0031-9155/51/20/N02
  • Reader Comments
  • © 2021 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Metrics

Article views(294) PDF downloads(48) Cited by(0)

Article outline

Figures and Tables

Figures(3)  /  Tables(2)

Other Articles By Authors

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return

Catalog