Peri-implantitis is a prevalent complication in dental implantology, often resulting from bacterial contamination on implant surfaces. Researchers have explored diode lasers as a promising alternative for bacterial decontamination. In this study, we evaluated the effectiveness of the 810 nm diode laser in eliminating Staphylococcus aureus from titanium implant surfaces. This investigation was designed as a controlled, standardized in vitro laboratory experiment. A total of 95 titanium implants were divided into the following groups: (1) A negative control group (n = 5): New integrity implants; (2) a positive control group (n = 6): Implants contaminated with a S. aureus suspension and left untreated; and (3) four intervention groups (n = 21 for each group): Implants contaminated and then treated with the 810 nm diode laser at varying power settings (1 W, 1.5 W, 2 W, 2.5 W) for 3 minutes. Bacterial load was quantified using colony-forming unit analysis, while surface roughness and morphology were assessed with a surface roughness tester and scanning electron microscopy (SEM) after laser treatment. The results showed that all intervention groups demonstrated significant reductions in bacterial load compared to the positive control group, with bacterial reduction increasing with higher laser power. The 2.5 W settings proved most effective in decontamination, although none of the laser settings completely eradicated the bacteria. Laser treatment did not result in significant changes in implant surface roughness, suggesting its safety for use in surface decontamination without compromising implant integrity. This technology may represent a safer, more effective approach to implant surface decontamination than traditional methods.
Citation: Thuy Vo, Loan Pham, Luong Dau, Tien Ho, Duc Cao, Lam Bui. Utilization of an 810 nm diode laser for the eradication of Staphylococcus aureus on implant surfaces: an in vitro study[J]. AIMS Bioengineering, 2026, 13(2): 281-294. doi: 10.3934/bioeng.2026013
Peri-implantitis is a prevalent complication in dental implantology, often resulting from bacterial contamination on implant surfaces. Researchers have explored diode lasers as a promising alternative for bacterial decontamination. In this study, we evaluated the effectiveness of the 810 nm diode laser in eliminating Staphylococcus aureus from titanium implant surfaces. This investigation was designed as a controlled, standardized in vitro laboratory experiment. A total of 95 titanium implants were divided into the following groups: (1) A negative control group (n = 5): New integrity implants; (2) a positive control group (n = 6): Implants contaminated with a S. aureus suspension and left untreated; and (3) four intervention groups (n = 21 for each group): Implants contaminated and then treated with the 810 nm diode laser at varying power settings (1 W, 1.5 W, 2 W, 2.5 W) for 3 minutes. Bacterial load was quantified using colony-forming unit analysis, while surface roughness and morphology were assessed with a surface roughness tester and scanning electron microscopy (SEM) after laser treatment. The results showed that all intervention groups demonstrated significant reductions in bacterial load compared to the positive control group, with bacterial reduction increasing with higher laser power. The 2.5 W settings proved most effective in decontamination, although none of the laser settings completely eradicated the bacteria. Laser treatment did not result in significant changes in implant surface roughness, suggesting its safety for use in surface decontamination without compromising implant integrity. This technology may represent a safer, more effective approach to implant surface decontamination than traditional methods.
| [1] |
French D, Ofec R, Levin L (2021) Long term clinical performance of 10 871 dental implants with up to 22 years of follow-up: a cohort study in 4247 patients. Clin Implant Dent Relat Res 23: 289-297. https://doi.org/10.1111/cid.12994 
|
| [2] |
Rakic M, Galindo-Moreno P, Monje A, et al. (2018) How frequent does peri-implantitis occur? A systematic review and meta-analysis. Clin Oral Investig 22: 1805-1816. https://doi.org/10.1007/s00784-017-2276-y
|
| [3] |
Ting M, Craig J, Balkin BE, et al. (2018) Peri-implantitis: a comprehensive overview of systematic reviews. J Oral Implantol 44: 225-247. https://doi.org/10.1563/aaid-joi-D-16-00122
|
| [4] | Kadirvelu L, Sivaramalingam SS, Jothivel D, et al. (2024) A review on antimicrobial strategies in mitigating biofilm-associated infections on medical implants. Curr Res Microb Sci 6: 100231. https://doi.org/10.1016/j.crmicr.2024.100231 |
| [5] |
Quirynen M, Vogels R, Peeters W, et al. (2006) Dynamics of initial subgingival colonization of ‘pristine’ peri-implant pockets. Clin Oral Implants Res 17: 25-37. https://doi.org/10.1111/j.1600-0501.2005.01194.x
|
| [6] |
Furst MM, Salvi GE, Lang NP, et al. (2007) Bacterial colonization immediately after installation on oral titanium implants. Clin Oral Implants Res 18: 501-508. https://doi.org/10.1111/j.1600-0501.2007.01381.x
|
| [7] |
Kronström M SB, Erickson E, Houston L, et al. (2000) Humoral immunity host factors in subjects with failing or successful titanium dental implants. J Clin Periodontol 27: 875-882. https://doi.org/10.1034/j.1600-051x.2000.027012875.x
|
| [8] |
Persson GR, Renvert S (2014) Cluster of bacteria associated with peri-implantitis. Clin Implant Dent Relat Res 16: 783-793. https://doi.org/10.1111/cid.12052
|
| [9] |
Roccuzzo A, Stähli A, Monje A, et al. (2021) Peri-implantitis: a clinical update on prevalence and surgical treatment outcomes. J Clin Med 10: 1107. https://doi.org/10.3390/jcm10051107
|
| [10] |
Herrera D, Berglundh T, Schwarz F, et al. (2023) Prevention and treatment of peri-implant diseases-The EFP S3 level clinical practice guideline. J Clin Periodontol 50: 4-76. https://doi.org/10.1111/jcpe.13823
|
| [11] |
Baima G, Citterio F, Romandini M, et al. (2022) Surface decontamination protocols for surgical treatment of peri-implantitis: a systematic review with meta-analysis. Clin Oral Implants Res 33: 1069-1086. https://doi.org/10.1111/clr.13992
|
| [12] |
Mellado-Valero A, Buitrago-Vera P, Sola-Ruiz MF, et al. (2013) Decontamination of dental implant surface in peri-implantitis treatment: a literature review. Med Oral Patol Oral Cir Bucal 18: e869-e876. https://doi.org/10.4317/medoral.19420
|
| [13] |
Marotti J, Tortamano P, Cai S, et al. (2013) Decontamination of dental implant surfaces by means of photodynamic therapy. Lasers Med Sci 28: 303-309. https://doi.org/10.1007/s10103-012-1148-6
|
| [14] |
Valente NA, Mang T, Hatton M, et al. (2017) Effects of two diode lasers with and without photosensitization on contaminated implant surfaces: an ex vivo study. Photomed Laser Surg 35: 347-356. https://doi.org/10.1089/pho.2016.4247
|
| [15] |
Romanos GE, Gutknecht N, Dieter S, et al. (2009) Laser wavelengths and oral implantology. Lasers Med Sci 24: 961-970. https://doi.org/10.1007/s10103-009-0676-1
|
| [16] |
Ting M, Alluri LSC, Sulewski JG, et al. (2022) Laser treatment of peri-implantitis: a systematic review of radiographic outcomes. Dent J 10: 20. https://doi.org/10.3390/dj10020020
|
| [17] |
Tosun E, Tasar F, Strauss R, et al. (2012) Comparative evaluation of antimicrobial effects of Er:YAG, diode, and CO2 lasers on titanium discs: an experimental study. J Oral Maxillofac Surg 70: 1064-1069. https://doi.org/10.1016/j.joms.2011.11.021
|
| [18] |
Gonçalves F, Zanetti A L, Zanetti R V, et al. (2010) Effectiveness of 980-mm diode and 1064-nm extra-long-pulse neodymium-doped yttrium aluminum garnet lasers in implant disinfection. Photomed Laser Surg 28: 273-280. https://doi.org/10.1089/pho.2009.2496
|
| [19] | Azma E, Safavi N (2013) Diode laser application in soft tissue oral surgery. J Lasers Med Sci 4: 206-211. |
| [20] | Ortega-Concepción D, Cano-Durán JA, Peña-Cardelles JF, et al. (2017) The application of diode laser in the treatment of oral soft tissues lesions. A literature review. J Clin Exp Dent 9: e925-e928. https://doi.org/10.4317/jced.53795 |
| [21] |
Roncati M, Lucchese A, Carinci F (2013) Non-surgical treatment of peri-implantitis with the adjunctive use of an 810-nm diode laser. J Indian Soc Periodontol 17: 812-815. https://doi.org/10.4103/0972-124X.124531
|
| [22] |
Saffarpour A, Nozari A, Fekrazad R, et al. (2018) Microstructural evaluation of contaminated implant surface treated by laser, photodynamic therapy, and chlorhexidine 2 percent. Int J Oral Maxillofac Implants 33: 1019-1026. https://doi.org/10.11607/jomi.6325
|
| [23] |
Kushima SS, Nagasawa M, Shibli JA, et al. (2016) Evaluation of temperature and roughness alteration of diode laser irradiation of zirconia and titanium for peri-implantitis treatment. Photomed Laser Surg 34: 194-199. https://doi.org/10.1089/pho.2015.4026
|
| [24] |
Castro GL, Gallas M, Nunez IR, et al. (2007) Scanning electron microscopic analysis of diode laser-treated titanium implant surfaces. Photomed Laser Surg 25: 124-128. https://doi.org/10.1089/pho.2006.1086
|
| [25] |
Monzavi A, Fekrazad R, Chinipardaz Z, et al. (2018) Effect of Various Laser Wavelengths on Temperature Changes During Periimplantitis Treatment: an in vitro study. Implant Dent 27: 311-316. https://doi.org/10.1097/ID.0000000000000751
|
| [26] |
Faramarzi M, Sadighi M, Mirhashemi S (2018) Evaluation of surface changes of dental implants after irradiation with diode laser beams with different energies: a sem study. J Adv Periodontol Implant Dent 10: 85-89. https://doi.org/10.15171/japid.2018.013
|
| [27] |
Giannelli M, Landini G, Materassi F, et al. (2016) The effects of diode laser on Staphylococcus aureus biofilm and Escherichia coli lipopolysaccharide adherent to titanium oxide surface of dental implants. An in vitro study. Lasers Med Sci 31: 1613-1619. https://doi.org/10.1007/s10103-016-2025-5
|
| [28] | Kreisler M, Kohnen W, Marinello C, et al. (2003) Antimicrobial efficacy of semiconductor laser irradiation on implant surfaces. Int J Oral Maxillofac Implants 18: 706-711. |
| [29] |
Deppe H, Ahrens M, Behr AV, et al. (2021) Thermal effect of a 445 nm diode laser on five dental implant systems: an in vitro study. Sci Rep 11: 20174. https://doi.org/10.1038/s41598-021-99709-8
|
| [30] |
Giannelli M, Lasagni M, Bani D (2015) Thermal effects of lambda = 808 nm GaAlAs diode laser irradiation on different titanium surfaces. Lasers Med Sci 30: 2341-2352. https://doi.org/10.1007/s10103-015-1801-y
|
| [31] |
Geminiani A, Caton JG, Romanos GE (2012) Temperature change during non-contact diode laser irradiation of implant surfaces. Lasers Med Sci 27: 339-342. https://doi.org/10.1007/s10103-010-0876-8
|
| [32] |
Eriksson RA, Albrektsson T (1984) The effect of heat on bone regeneration: an experimental study in the rabbit using the bone growth chamber. J Oral Maxillofac Surg 42: 705-711. https://doi.org/10.1016/0278-2391(84)90417-8
|
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Thuy Vo, Loan Pham, Luong Dau, Tien Ho, Duc Cao, Lam Bui. Utilization of an 810 nm diode laser for the eradication of Staphylococcus aureus on implant surfaces: an in vitro study[J]. AIMS Bioengineering, 2026, 13(2): 281-294. doi: 10.3934/bioeng.2026013
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