Research article

Crackers enriched with Savoy cabbage and Annurca apple by-products: DPPH radical-scavenging activity, enzyme inhibition and predicted glycaemic index

  • Published: 19 October 2026
  • The growing demand for healthier and more sustainable food options is prompting the food sector to develop products that deliver nutritional benefits while reducing waste. This study examines a dual valorization strategy that integrates both vegetable and fruit by-products and underutilized seed oils into cracker formulations. Powders derived from Brassica oleracea var. sabauda (Savoy cabbage) by-products and Malus domestica var. Annurca peels were used to partially replace wheat flour (5% and 10%), while cold-pressed broccoli and cherry seed oils served as alternative lipid sources. This combined use of fiber- and polyphenol-rich powders with alternative seed oils represents a novel, circular approach to functional bakery design. Four experimental formulations (C1-C4) were prepared alongside a control, replacing wheat flour with 5% or 10% Brassica oleracea var. sabauda by-products plus broccoli seed oil (C1, C2) or with 5% or 10% Annurca apple peel powder plus cherry seed oil (C3, C4). Enrichment increased total polyphenol content and DPPH radical-scavenging activity compared with the control, with the highest values observed in the 10% Annurca peel formulation (C4; 267.7 μg GAE/g and 43.9%, respectively). The enriched crackers also showed lower IC50 values in the in vitro assays against α-amylase, α-glucosidase and pancreatic lipase, particularly C4, which exhibited the strongest inhibitory activity. In vitro starch digestion resulted in significantly lower predicted glycemic index (pGI) values in all enriched formulations compared with the control, with reductions ranging from approximately 17.5 to 23.8%. These findings indicate that incorporating plant-based by-products can modify the DPPH radical-scavenging activity, in vitro digestive enzyme inhibitory potential, and predicted glycemic response of crackers. Overall, the results support the potential of these by-products for the development of more resource-efficient bakery products, while further studies are needed to characterize individual and combined contributions of these ingredients.

    Citation: Maria Neve Ombra, Filomena Nazzaro, Florinda Fratianni. Crackers enriched with Savoy cabbage and Annurca apple by-products: DPPH radical-scavenging activity, enzyme inhibition and predicted glycaemic index[J]. AIMS Agriculture and Food, 2026, 11(4): 895-911. doi: 10.3934/agrfood.2026044

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  • The growing demand for healthier and more sustainable food options is prompting the food sector to develop products that deliver nutritional benefits while reducing waste. This study examines a dual valorization strategy that integrates both vegetable and fruit by-products and underutilized seed oils into cracker formulations. Powders derived from Brassica oleracea var. sabauda (Savoy cabbage) by-products and Malus domestica var. Annurca peels were used to partially replace wheat flour (5% and 10%), while cold-pressed broccoli and cherry seed oils served as alternative lipid sources. This combined use of fiber- and polyphenol-rich powders with alternative seed oils represents a novel, circular approach to functional bakery design. Four experimental formulations (C1-C4) were prepared alongside a control, replacing wheat flour with 5% or 10% Brassica oleracea var. sabauda by-products plus broccoli seed oil (C1, C2) or with 5% or 10% Annurca apple peel powder plus cherry seed oil (C3, C4). Enrichment increased total polyphenol content and DPPH radical-scavenging activity compared with the control, with the highest values observed in the 10% Annurca peel formulation (C4; 267.7 μg GAE/g and 43.9%, respectively). The enriched crackers also showed lower IC50 values in the in vitro assays against α-amylase, α-glucosidase and pancreatic lipase, particularly C4, which exhibited the strongest inhibitory activity. In vitro starch digestion resulted in significantly lower predicted glycemic index (pGI) values in all enriched formulations compared with the control, with reductions ranging from approximately 17.5 to 23.8%. These findings indicate that incorporating plant-based by-products can modify the DPPH radical-scavenging activity, in vitro digestive enzyme inhibitory potential, and predicted glycemic response of crackers. Overall, the results support the potential of these by-products for the development of more resource-efficient bakery products, while further studies are needed to characterize individual and combined contributions of these ingredients.



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    [1] Nagi HPS, Kaur J, Dar BN, et al. (2012) Effect of storage period and packaging on the shelf life of cereal bran incorporated biscuits. Am J Food Technol 7: 301–310. https://doi.org/10.3923/ajft.2012.301.310 doi: 10.3923/ajft.2012.301.310
    [2] Rico D, Ronda F, Villanueva M, et al. (2019) Development of healthy gluten-free crackers from white and brown tef (Eragrostis tef Zucc.) flours. Heliyon 5: e02598. https://doi.org/10.1016/j.heliyon.2019.e02598 doi: 10.1016/j.heliyon.2019.e02598
    [3] de Araújo FF, de Paulo Farias D, Neri-Numa IA, et al. (2021) Polyphenols and their applications: An approach in food chemistry and innovation potential. Food Chem 338: 127535.
    [4] Ștefan IA, Ona AD. (2020) Cabbage (Brassica oleracea L.) overview of the health benefits and therapeutical uses. Hop Med Plants 1: 150–169. https://doi.org/10.1016/j.foodchem.2020.127535 doi: 10.1016/j.foodchem.2020.127535
    [5] Fratianni F, Coppola R, Nazzaro F (2011) Phenolic composition and antimicrobial and antiquorum sensing activity of an ethanolic extract of peels from the apple cultivar Annurca. J Med Food 14: 957–963. https://doi.org/10.1089/jmf.2010.0170 doi: 10.1089/jmf.2010.0170
    [6] Choe U, Childs H, Zeng M, et al. (2022) Value-added utilization of fruit seed oils for improving human health: A progress review. ACS Food Sci Technol 3: 528. https://doi.org/10.1021/acsfoodscitech.2c00120 doi: 10.1021/acsfoodscitech.2c00120
    [7] Michalak M, Błońska-Sikora E, Dobros N, et al. (2024) Bioactive compounds, antioxidant properties, and cosmetic applications of selected cold-pressed plant oils from seeds. Cosmetics 11: 153. https://doi.org/10.3390/cosmetics11050153 doi: 10.3390/cosmetics11050153
    [8] Fratianni F, Amato G, De Feo V, et al. (2023) Potential therapeutic benefits of unconventional oils: assessment of the potential in vitro biological properties of some Rubiaceae, Cucurbitaceae, and Brassicaceae seed oils. Front Nutr 10: 1171766. https://doi.org/10.3389/fnut.2023.1171766 doi: 10.3389/fnut.2023.1171766
    [9] Fratianni F, d'Acierno A, Ombra MN, et al. (2021) Fatty acid composition, antioxidant, and in vitro anti-inflammatory activity of five cold-pressed Prunus seed oils, and their anti-biofilm effect against pathogenic bacteria. Front Nutr 8: 775751. https://doi.org/10.3389/fnut.2021.775751 doi: 10.3389/fnut.2021.775751
    [10] Barut YT, Koç GÇ, Akdoğan A, et al. (2025) Production and quality assessment of functional crackers based on fruit seed oils. J Food Meas Charact 19: 1751–1760. https://doi.org/10.1007/s11694-025-02345-7 doi: 10.1007/s11694-025-02345-7
    [11] Siddiqui AS, Anwar S, Yunusa BM, et al. (2023) The potential of apricot seed and oil as functional food: Composition, biological properties, health benefits & safety. Food Biosci 51: 102336. https://doi.org/10.1016/j.fbio.2023.102336 doi: 10.1016/j.fbio.2023.102336
    [12] Wang N, Maximiuk L, Fenn D, et al. (2020) Development of a method for determining oil absorption capacity in pulse flours and protein materials. Cereal Chem 97: 1111–1117. https://doi.org/10.1002/cche.10339 doi: 10.1002/cche.10339
    [13] Gani A, Wani SM, Masoodi FA, et al. (2013) Characterization of rice starches extracted from Indian cultivars. Food Sci Technol Int 19: 143–152. https://doi.org/10.1177/1082013212442189 doi: 10.1177/1082013212442189
    [14] Ombra MN, d'Acierno A, Nazzaro F, et al. (2018) Alpha-amylase, α-glucosidase and lipase inhibiting activities of polyphenol-rich extracts from six common bean cultivars of Southern Italy, before and after cooking. Int J Food Sci Nutr 69: 824–834. https://doi.org/10.1080/09637486.2017.1418845 doi: 10.1080/09637486.2017.1418845
    [15] Bustos MC, Vignola MB, Paesani C, et al. (2020) Berry fruits-enriched pasta: Effect of processing and in vitro digestion on phenolics and its antioxidant activity, bioaccessibility and potential bioavailability. Int J Food Sci Technol 55: 2104–2112. https://doi.org/10.1111/ijfs.14453 doi: 10.1111/ijfs.14453
    [16] Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 28: 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5 doi: 10.1016/S0023-6438(95)80008-5
    [17] Nickavar B, Abolhasani L, Izadpanah H (2008) α-Amylase inhibitory activities of six Salvia species. Iran J Pharm Res 7: 297–303. https://doi.org/10.22037/ijpr.2010.779 doi: 10.22037/ijpr.2010.779
    [18] Sharp H, Hollinshead J, Bartholomew BB, et al. (2007) Inhibitory effects of Cissus quadrangularis L. derived components on lipase, amylase and α-glucosidase activity in vitro. Nat Prod Commun 2: 817–822. https://doi.org/10.1177/1934578X0700200806 doi: 10.1177/1934578X0700200806
    [19] Brennan CS, Tudorica CM (2008) Evaluation of potential mechanisms by which dietary fibre additions reduce the predicted glycaemic index of fresh pastas. Int J Food Sci Technol 12: 2151–2162. https://doi.org/10.1111/j.1365-2621.2008.01831.x doi: 10.1111/j.1365-2621.2008.01831.x
    [20] Ombra MN, Nazzaro F, Fratianni F (2022) Enriched pasta incorporating typical vegetables of mediterranean diet: in vitro evaluation of inhibitory potential on digestive enzymes and predicted glycaemic index. Int J Food Sci Nutr 19: 1–8. https://doi.org/10.1080/09637486.2022.2158180 doi: 10.1080/09637486.2022.2158180
    [21] Smith WT, Cheng C. (1978) Use of 3, 5-dinitrosalicylate reagent for glucose determination in mixed solvents. Anal Lett 11: 191–194. https://doi.org/10.1080/00032717808067867 doi: 10.1080/00032717808067867
    [22] Brennan CS, Tudorica CM (2007) Fresh pasta quality as affected by enrichment of nonstarch polysaccharides. J Food Sci 72: S659–S665. https://doi.org/10.1111/j.1750-3841.2007.00541.x doi: 10.1111/j.1750-3841.2007.00541.x
    [23] Ombra MN, Nazzaro F, Fratianni F (2025) Reduced predicted glycaemic response and inhibitory effects on lipid-digesting enzymes of pasta enriched with flour of Malus domestica "Annurca cv. " pulp or peel. Int J Food Sci 1: 8361330. https://doi.org/10.1155/ijfo/8361330 doi: 10.1155/ijfo/8361330
    [24] Ombra MN, Cozzolino A, Nazzaro F, et al. (2017) Biochemical and biological characterization of two Brassicaceae after their commercial expiry date. Food Chem 218: 335–340. https://doi.org/10.1016/j.foodchem.2016.09.082 doi: 10.1016/j.foodchem.2016.09.082
    [25] Reaz AH, Abedin MJ, Abdullah ATM, et al. (2023) Physicochemical and structural impact of CMC-hydrocolloids on the development of gluten-free foxtail millet biscuits. Heliyon 9: e17176. https://doi.org/10.1016/j.heliyon.2023.e17176 doi: 10.1016/j.heliyon.2023.e17176
    [26] Meriles SP, Piloni R, Cáceres GV, et al. (2022) Compositional characteristics, texture, shelf-life and sensory quality of snack crackers produced from non-traditional ingredients. Int J Food Sci Technol 57: 4689–4696. https://doi.org/10.1111/ijfs.15303 doi: 10.1111/ijfs.15303
    [27] Sattar DES, Ali TM, Soomro UA, et al. (2023) Functional, antioxidant, and sensory properties of a ready-to-eat wheat flour snack incorporated with germinated legume flour. Legum Sci 5: e174. https://doi.org/10.1002/leg3.174 doi: 10.1002/leg3.174
    [28] Zhang B, Zhang Y, Li H, et al. (2020) A review on insoluble-bound phenolics in plant-based food matrix and their contribution to human health with future perspectives. Trends Food Sci Technol 105: 347–362. https://doi.org/10.1016/j.tifs.2020.09.029 doi: 10.1016/j.tifs.2020.09.029
    [29] Adelerin RO, Awolu OO, Ifesan BOT, et al. (2024) Pumpkin-based cookies formulated from optimized pumpkin flour blends: Nutritional and antidiabetic potentials. Food Human 2: 100215. https://doi.org/10.1016/j.foohum.2023.100215 doi: 10.1016/j.foohum.2023.100215
    [30] Al-Saab AH, Gadallah MGE (2021) Phytochemicals, antioxidant activity and quality properties of fibre enriched cookies incorporated with orange peel powder. Food Res 5: 72–79. https://doi.org/10.26656/fr.2017.5(4).698 doi: 10.26656/fr.2017.5(4).698
    [31] Zhang G, Wang Y, Liang Z, et al. (2025) Nutritional value and advances of mealworm (Tenebrio molitor) as a sustainable functional ingredient in bakery products: A key step toward sustainable diets. Future Foods 12: 100716. https://doi.org/10.1016/j.fufo.2025.100716 doi: 10.1016/j.fufo.2025.100716
    [32] Mofidipour M, Fadaei V, Salehifar M (2025) Inhibition of acrylamide and α-amylase and α-glucosidase activities in Echium amoenum powder fortified biscuits. Food Res Int 200: 115462. https://doi.org/10.1016/j.foodres.2025.115462 doi: 10.1016/j.foodres.2025.115462
    [33] Devi R, Sarma MP, Goudar G, et al. (2024) Nutraceutical enrichment of cracker with incorporation of bamboo shoot and green gram flour. Nutr Food Sci 12: 1272–1283. https://doi.org/10.12944/CRNFSJ.12.3.5 doi: 10.12944/CRNFSJ.12.3.5
    [34] Uddin M, Mashahid N, Anta JA, et al. (2025) Development and evaluation of functional biscuits infused with functional spices and white pea flour: A comprehensive study on optimizing glycemic control and incorporating anti-diabetic additives. Appl Food Res 5: 100710. https://doi.org/10.1016/j.afres.2025.100710 doi: 10.1016/j.afres.2025.100710
    [35] Olagunju AI, Omoba OS, Enujiugha VN, et al. (2018) Development of value-added nutritious crackers with high antidiabetic properties from blends of Acha (Digitaria exilis) and blanched Pigeon pea (Cajanus cajan). Food Sci Nutr 6: 1791–1802. https://doi.org/10.1002/fsn3.748 doi: 10.1002/fsn3.748
    [36] Sharma V, Devkota L, Kishore N, et al. (2025) Understanding the interplay between dietary fiber, polyphenols, and digestive enzymes. Food Hydrocolloids 166: 111310. https://doi.org/10.1016/j.foodhyd.2025.111310 doi: 10.1016/j.foodhyd.2025.111310
    [37] Wronkowska M, Zieliński H, Bączek N, et al. (2024) Retention of bioactive compounds during technological steps of the production of bread enriched with roasted buckwheat hulls. Food Chem 460: 140645. https://doi.org/10.1016/j.foodchem.2024.140645 doi: 10.1016/j.foodchem.2024.140645
    [38] Brennan CS, Tudorica CM (2008) Evaluation of potential mechanisms by which dietary fibre additions reduce the predicted glycaemic index of fresh pastas. Int J Food Sci Technol 43: 2151–2162. https://doi.org/10.1111/j.1365-2621.2008.01831.x doi: 10.1111/j.1365-2621.2008.01831.x
    [39] Di Pede G, Dodi R, Scarpa C, et al. (2021) Glycemic index values of pasta products: An overview. Food 10: 2541. https://doi.org/10.3390/foods10112541 doi: 10.3390/foods10112541
    [40] Millán-Laleona A, Bielsa F, Aranda-Cañada E, et al. (2023) Antioxidant, antidiabetic, and anti-obesity properties of apple pulp extracts (Malus domestica Bork): A comparative study of 15 local and commercial cultivars from Spain. Biology 12: 891. https://doi.org/10.3390/biology12060891 doi: 10.3390/biology12060891
    [41] Frankenfeld CL, Leslie TF, Makara MA (2015) Diabetes, obesity, and recommended fruit and vegetable consumption in relation to food environment sub-types: A cross-sectional analysis of Behavioral Risk Factor Surveillance System, United States Census, and food establishment data. BMC Public Health 15: 491. https://doi.org/10.1186/s12889-015-1819-x doi: 10.1186/s12889-015-1819-x
    [42] Gallardo MA, Martínez-Navarro ME, García Panadero I, et al. (2025) Nutritional enhancement of crackers through the incorporation of by-products from the frozen pumpkin industry. Foods 14: 2548. https://doi.org/10.3390/foods14142548 doi: 10.3390/foods14142548
    [43] Ashraf SA (2025) Food fortification as a sustainable global strategy to mitigate micronutrient deficiencies and improve public health. Discov Food 5: 201. https://doi.org/10.1007/s44187-025-00512-5 doi: 10.1007/s44187-025-00512-5
    [44] Di Cairano M, Tchuenbou-Magaia FL, Condelli N, et al. (2022) Glycaemic index of gluten-free biscuits with resistant starch and sucrose replacers: An in vivo and in vitro comparative study. Foods 11: 3253. https://doi.org/10.3390/foods11203253 doi: 10.3390/foods11203253
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