Industrial hemp (Cannabis sativa L.) is a sustainable, high-yielding crop, well adapted to the Mediterranean climate, but little information exists concerning the performance of industrial hemp varieties in the field under common management practices. In this study, we evaluated the agronomic performance of two monoecious industrial hemp varieties, Ferimon and Fedora 17, across two distinct Greek locations (Thermi and Evros) over two consecutive growing seasons (2023 and 2024). The varieties were assessed for key morphological traits, including plant height, stem diameter, internode length, SPAD index, along with biomass production, fiber content, and seed yield. Significant annual variation in industrial hemp growth was noted, with plants in 2023 exhibiting greater plant height (215 vs. 130 cm), stem diameter (9.32 vs. 7.29 mm), internode length (16.9 vs. 10.5 cm), and SPAD readings (49.6 vs. 42.6) compared to 2024. While location had a limited effect on plant height, it significantly influenced other traits, with Evros consistently favoring higher SPAD readings (51.4 vs. 40.7) and longer internodes (14.3 vs. 13.2 cm). Ferimon exhibited superior plant height (178 cm) and seed yield (0.664 t ha−1) across most settings, whereas Fedora 17 demonstrated enhanced fiber content (25.9%) and stem diameter (8.9 mm), especially in Thermi. Industrial hemp plants exhibited Δ9-tetrahydrocannabinol (THC) levels between 0.10% and 0.12%, consistently below the 0.3% EU regulatory threshold, demonstrating successful compliance within Mediterranean cultivation systems. However, the presence of three-way interactions (year x location x variety) for most agronomic parameters highlighted the need for environment-specific cultivar recommendations. The findings provide valuable insights into varietal adaptability of industrial hemp in Greece and contribute to strategic decision-making in industrial hemp cultivation in Mediterranean environments.
Citation: E. Tsaliki, T. Tzivara, C.A. Damalas, S. Fotiadis, E. Vazanelli, I. Panoras, T. Moysiadis, S.D. Koutroubas. Agronomic performance of industrial hemp (Cannabis sativa L.) in northern Greece: A comparative study[J]. AIMS Agriculture and Food, 2025, 10(3): 744-755. doi: 10.3934/agrfood.2025038
Industrial hemp (Cannabis sativa L.) is a sustainable, high-yielding crop, well adapted to the Mediterranean climate, but little information exists concerning the performance of industrial hemp varieties in the field under common management practices. In this study, we evaluated the agronomic performance of two monoecious industrial hemp varieties, Ferimon and Fedora 17, across two distinct Greek locations (Thermi and Evros) over two consecutive growing seasons (2023 and 2024). The varieties were assessed for key morphological traits, including plant height, stem diameter, internode length, SPAD index, along with biomass production, fiber content, and seed yield. Significant annual variation in industrial hemp growth was noted, with plants in 2023 exhibiting greater plant height (215 vs. 130 cm), stem diameter (9.32 vs. 7.29 mm), internode length (16.9 vs. 10.5 cm), and SPAD readings (49.6 vs. 42.6) compared to 2024. While location had a limited effect on plant height, it significantly influenced other traits, with Evros consistently favoring higher SPAD readings (51.4 vs. 40.7) and longer internodes (14.3 vs. 13.2 cm). Ferimon exhibited superior plant height (178 cm) and seed yield (0.664 t ha−1) across most settings, whereas Fedora 17 demonstrated enhanced fiber content (25.9%) and stem diameter (8.9 mm), especially in Thermi. Industrial hemp plants exhibited Δ9-tetrahydrocannabinol (THC) levels between 0.10% and 0.12%, consistently below the 0.3% EU regulatory threshold, demonstrating successful compliance within Mediterranean cultivation systems. However, the presence of three-way interactions (year x location x variety) for most agronomic parameters highlighted the need for environment-specific cultivar recommendations. The findings provide valuable insights into varietal adaptability of industrial hemp in Greece and contribute to strategic decision-making in industrial hemp cultivation in Mediterranean environments.
| [1] |
Kaur G, Kander R (2023) The sustainability of industrial hemp: A literature review of its economic, environmental, and social sustainability. Sustainability 15: 6457. https://doi.org/10.3390/su15086457 doi: 10.3390/su15086457
|
| [2] |
Ahmed ATMF, Islam MZ, Mahmud MS, et al. (2022) Hemp as a potential raw material toward a sustainable world: A review. Heliyon 8: e08753. https://doi.org/10.1016/j.heliyon.2022.e08753 doi: 10.1016/j.heliyon.2022.e08753
|
| [3] |
Tsaliki E, Kalivas A (2024) Hemp crop opportunities. Ann Agric Crop Sci 9: 1154. https://doi.org/10.26420/annagriccropsci.2024.1154 doi: 10.26420/annagriccropsci.2024.1154
|
| [4] | European Commission, Regulation (EC) (2022) No. 126/2022 of 7 December 2021. Available from: https://eur-lex.europa.eu/legal-content/EN/ALL/?uri = CELEX%3A32022R0126. |
| [5] | European Commission (2025) Hemp production in the EU. Agriculture and Rural Development. Available from: https://agriculture.ec.europa.eu/farming/crop-productions-and-plant-based-products/hemp_en. |
| [6] |
Baldini M, Ferfuia C, Zuliani F, et al. (2020) Suitability assessment of different hemp (Cannabis sativa L.) varieties to the cultivation environment. Ind Crops Prod 143: 111860. https://doi.org/10.1016/j.indcrop.2019.111860 doi: 10.1016/j.indcrop.2019.111860
|
| [7] |
Visković J, Zheljazkov VD, Sikora V, et al. (2023) Industrial hemp (Cannabis sativa L.) agronomy and utilization: A review. Agronomy 13: 931. https://doi.org/10.3390/agronomy13030931 doi: 10.3390/agronomy13030931
|
| [8] |
Sunoj Valiaparambil SJ, Dong X, Trostle C, et al. (2023) Hemp agronomy: Current advances, questions, challenges, and opportunities. Agronomy 13: 475. https://doi.org/10.3390/agronomy13020475 doi: 10.3390/agronomy13020475
|
| [9] |
Amaducci S, Scordia D, Liu FH, et al. (2015) Key cultivation techniques for hemp in Europe and China. Ind Crops Prod 68: 2–16. https://doi.org/10.1016/j.indcrop.2014.06.041 doi: 10.1016/j.indcrop.2014.06.041
|
| [10] |
Adesina I, Bhowmik A, Sharma H, et al. (2020) A review on the current state of knowledge of growing conditions, agronomic soil health practices and utilities of hemp in the United States. Agriculture 10: 129. https://doi.org/10.3390/agriculture10040129 doi: 10.3390/agriculture10040129
|
| [11] |
Campiglia E, Radicetti E, Mancinelli R (2017) Plant density and nitrogen fertilization affect agronomic performance of industrial hemp (Cannabis sativa L.) in Mediterranean environment. Ind Crops Prod 100: 246–254. https://doi.org/10.1016/j.indcrop.2017.02.022 doi: 10.1016/j.indcrop.2017.02.022
|
| [12] |
Kalousek P, Holátko J, Schreiber P, et al. (2024). The effect of chelating agents on the Zn-phytoextraction potential of hemp and soil microbial activity. Chem Biol Technol Agric 11: 23. https://doi.org/10.1186/s40538-024-00544-6 doi: 10.1186/s40538-024-00544-6
|
| [13] |
Burton RA, Andres M, Cole M, et al. (2022) Industrial hemp seed: From the field to value-added food ingredients. J Cannabis Res 4: 45. https://doi.org/10.1186/s42238-022-00156-7 doi: 10.1186/s42238-022-00156-7
|
| [14] |
Irakli M, Tsaliki E, Kalivas A, et al. (2019) Effect of genotype and growing year on the nutritional, phytochemical, and antioxidant properties of industrial hemp (Cannabis sativa L.) seeds. Antioxidants 8: 491. https://doi.org/10.3390/antiox8100491 doi: 10.3390/antiox8100491
|
| [15] |
Farinon B, Molinari R, Costantini L, et al. (2020) The seed of industrial hemp (Cannabis sativa L.): Nutritional quality and potential functionality for human health and nutrition. Nutrients 12: 1935. https://doi.org/10.3390/nu12071935 doi: 10.3390/nu12071935
|
| [16] |
Šťastník O, Jůzl M, Karásek F, et al. (2019) The effect of hempseed expellers on selected quality indicators of broiler chicken's meat. Acta Veterinaria Brno 88: 121–128. https://doi.org/10.2754/avb201988010121 doi: 10.2754/avb201988010121
|
| [17] |
Tsaliki E, Kalivas A, Jankauskiene Z, et al. (2021) Fibre and seed productivity of industrial hemp (Cannabis sativa L.) varieties under Mediterranean conditions. Agronomy 11: 17. https://doi.org/10.3390/agronomy11010171 doi: 10.3390/agronomy11010171
|
| [18] | iHemp Farms (2025) iHemp Farms: Your Source for Quality Hemp Products. Available from: http://www.ihempfarms.com. |
| [19] | Rowell DL (1994) Soil science: Methods and applications. Longman Group UK Ltd., London. |
| [20] | Mediavilla V, Jonquera M, Schmid-Slembrouck I, et al. (1998) Decimal code for growth stages of hemp (Cannabis sativa L.). J Int Hemp Ass 5: 67–72. |
| [21] | UPOV (2022) Protocol for tests on distinctness, uniformity and stability Cannabis sativa L. HEMP, CANNABIS. CPVO-TP/276/2-Rev. Available from: https://www.upov.int/edocs/tgdocs/en/tg276.pdf. |
| [22] |
Baldini M, Ferfuia C, Piani B, et al. (2018) The performance and potentiality of monoecious hemp (Cannabis sativa L.) cultivars as a multipurpose crop. Agronomy 8: 162. https://doi.org/10.3390/agronomy8090162 doi: 10.3390/agronomy8090162
|
| [23] |
Mendel P, Schiavo-Capri E, Lalge AB, et al. (2020) Evaluation of selected characteristics in industrial hemp after phytohormonal treatment. Pak J Agric Sci 57: 1–7. https://doi.org/10.21162/PAKJAS/20.7586 doi: 10.21162/PAKJAS/20.7586
|
| [24] |
Livingstone H, Ang TN, Yuan X, et al. (2022) Analysis of inter-nodal properties of two industrial hemp cultivars (Fasamo and Ferimon 12) and their relationships with plant density and row spacing. Ind Crops Prod 182: 114880. https://doi.org/10.1016/j.indcrop.2022.114880 doi: 10.1016/j.indcrop.2022.114880
|
| [25] |
Hammami N, Privé JP, Moreau G (2022) Spatiotemporal variability and sensitivity of industrial hemp cultivars under variable field conditions. Eur J Agron 138: 126549. https://doi.org/10.1016/j.eja.2022.126549 doi: 10.1016/j.eja.2022.126549
|
| [26] |
Tang K, Struik PC, Yin X, et al. (2016) Comparing hemp (Cannabis sativa L.) cultivars for dual-purpose production under contrasting environments. Ind Crops Prod 87: 33–44. https://doi.org/10.1016/j.indcrop.2016.04.026 doi: 10.1016/j.indcrop.2016.04.026
|
| [27] |
Sausserde R, Adamovics A (2013) Industrial hemp for biomass production. J Agric Eng 44: e123. https://doi.org/10.4081/jae.2013.365 doi: 10.4081/jae.2013.365
|
| [28] |
Baraniecki P, Latterini F, Stefanoni W, et al. (2022) Assessment of the working performance of an innovative prototype to harvest hemp seed in two different conditions of terrain slope. Agronomy 12: 185. https://doi.org/10.3390/agronomy12010185 doi: 10.3390/agronomy12010185
|
| [29] |
Tsaliki E, Moysiadis T, Kalivas A, et al. (2024) Multi-year insights into industrial hemp growth in Mediterranean climate. Agronomy 14: 1946. https://doi.org/10.3390/agronomy14091946 doi: 10.3390/agronomy14091946
|
| [30] |
Assirelli A, Santangelo E, Stagno F, et al. (2022) Hemp sowing seed production: Assessment of new approaches in North-Italy. Sustainability 14: 17020. https://doi.org/10.3390/su142417020 doi: 10.3390/su142417020
|
| [31] |
Rahemi A, Dhakal R, Temu VW, et al. (2021) Performance of different-use type industrial hemp cultivars under Mid-Atlantic region conditions. Agronomy 11: 2321. https://doi.org/10.3390/agronomy11112321 doi: 10.3390/agronomy11112321
|
| [32] |
Panday D, Acharya BS, Dhakal M, et al. (2025) Industrial hemp yield and chemical composition as influenced by row spacing, fertilization, and environmental conditions. Agrosyst Geo Environ 8: e70093. https://doi.org/10.1002/agg2.70093 doi: 10.1002/agg2.70093
|