An effective approach for targeting lymphoma and leukemia cell lines with a novel Tan-CAR (CD30/CD20) T cell

Rimjhim Mohanty; Niladri Ganguly; Rimjhim Mohanty; Niladri Ganguly

doi:10.3934/Allergy.2026008

AIMS Allergy and Immunology

2026, Volume 10, Issue 2: 83-107. doi: 10.3934/Allergy.2026008

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Research article

An effective approach for targeting lymphoma and leukemia cell lines with a novel Tan-CAR (CD30/CD20) T cell

Rimjhim Mohanty ^,,
Niladri Ganguly

School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar, 751024, India

Received: 15 February 2026 Revised: 19 May 2026 Accepted: 26 May 2026 Published: 11 June 2026

Despite the success of single-target antigen CART therapy against CD19, there are certain limitations that need to be investigated properly to build a universal treatment for hematologic cancer. Other than CD19, there have been many reports on CD30 and CD20, which are also clinically relevant in different types of hematologic malignancies, but no reports have been published yet that target both antigens as bispecific targets on a single cell. Therefore, we developed a tandem receptor that targets dual antigens with a single receptor and likely reduces tumor marker escape mutations. In this study, we employed a gene editing strategy to disrupt TCRs and a target-specific integration of CAR receptors into T cells to develop a novel CAR model to address tumor-off-target limitations.
- CAR T cell therapy,
- third-generation CAR receptor,
- tandem-CAR,
- bispecific targets,
- CD20,
- CD30,
- CRISPR/CasS9
Citation: Rimjhim Mohanty, Niladri Ganguly. An effective approach for targeting lymphoma and leukemia cell lines with a novel Tan-CAR (CD30/CD20) T cell[J]. AIMS Allergy and Immunology, 2026, 10(2): 83-107. doi: 10.3934/Allergy.2026008

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Abstract

Despite the success of single-target antigen CART therapy against CD19, there are certain limitations that need to be investigated properly to build a universal treatment for hematologic cancer. Other than CD19, there have been many reports on CD30 and CD20, which are also clinically relevant in different types of hematologic malignancies, but no reports have been published yet that target both antigens as bispecific targets on a single cell. Therefore, we developed a tandem receptor that targets dual antigens with a single receptor and likely reduces tumor marker escape mutations. In this study, we employed a gene editing strategy to disrupt TCRs and a target-specific integration of CAR receptors into T cells to develop a novel CAR model to address tumor-off-target limitations.

Abbreviations

CAR

Chimeric antigen receptor

CTLA-4

Cytotoxic T Lymphocyte-associated protein 4

SHP-1

Src homology domain containing tyrosine phosphatase 1

GM-CSF

Granulocyte-macrophage colony stimulating factor

HPSC

Hematopoietic stem cell

FasR

Fas receptor

LAG-3

Lymphocyte activation gene 3

iPSC

Induced pluripotent stem cell

PD-1

Programmed death-1

TGF-βR2

Tumor growth factor β receptor 2

TCR

T-cell receptor

Acknowledgments

I would like to express my gratitude to the funding agency DST, New Delhi, INDIA for approving me DST Women Scientist Grant and also KIIT University for providing me the scientific facilities and infrastructure to carry out the research work. I would also like to give special thanks to my colleague Dr. Deepak ku Ojha for drawing blood samples from healthy donors and all the generous persons had came forward to donate their blood samples required for this study.

Author contributions

RM—Conceived the project idea, Grant Writing, Funding Acquisition, Conceptualization, Experimental Design, Investigation and Data Curation, Writing- Original draft.
NG—Methodology Preparation, Project Administration, Validation, Data Interpretation, Review and Editing and Approved the final manuscript.

Conflicts of interest

The authors declare no conflict of interest.

Ethics approval status

This study has been approved by the institutional ethical committee of KIIT School of Biotechnology (KSBT/IEC/2019/MEET-1/A7). A written consent was obtained from all participants who voluntarily involve for this study and a small compensation was provided to all participants for their contribution.

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