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Apomorphine-induced pathway perturbation in MPP+-treated SH-SY5Y cells

Department of Biomolecular and Chemical Engineering, DongYang University, Yeongju 36040, South Korea

Apomorphine (APOM) is a non-selective dopamine agonist for Parkinson’s disease (PD). It also offers protection against oxidative stress. Thus, it has been used for treating advanced PD patients who do not respond to levodopa or other dopamine agonists. However, side effects such as orthostatic hypotension, nausea, and fibrotic nodules at the site of APOM injection have been reported after long-term use of APOM in PD patients. To secure the use of APOM for PD treatment without side effect, it is essential to understand the molecular mechanism involved in the action of APOM in PD. In this study, gene expression profile changes by APOM in a PD cell model, i.e., MPP+-treated SH-SY5Y cells, were measured at six time points (0, 3, 6, 9, 12, and 24 h) after APOM treatment using a commercial whole-genome expression array. A total of 2249 genes showed significant and differential expression profile. Pathways significantly affected by APOM were estimated using signaling pathway impact analysis (SPIA). In addition, differentially regulated regions within each affected pathway were identified with covariance analysis using a structure equation model.
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Keywords apomorphine; gene expression; structure equation model; signaling pathway analysis; SH-SY5Y cells; Parkinson’s disease

Citation: Jin Hwan Do. Apomorphine-induced pathway perturbation in MPP+-treated SH-SY5Y cells. AIMS Molecular Science, 2017, 4(3): 271-287. doi: 10.3934/molsci.2017.3.271


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This article has been cited by

  • 1. Jin Hwan Do, Correction: Apomorphine-induced pathway perturbation in MPP+-treated SH-SY5Y cells, AIMS Molecular Science, 2017, 4, 4, 445, 10.3934/molsci.2017.4.445
  • 2. Daniele Pepe, Jin Hwan Do, Analyzing Apomorphine-Mediated Effects in a Cell Model for Parkinson's Disease with Partial Least Squares Structure Equation Modeling, Journal of Computational Biology, 2019, 10.1089/cmb.2019.0386

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Copyright Info: 2017, Jin Hwan Do, licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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