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Selective adhesion of wastewater bacteria to Pleurotus ostreatus mycelium in a trickle-bed bioreactor

1 Laboratory of Environmental Biotechnology, Institute of Microbiology of the CAS, v.v.i., Vídeňská 1083, 14220 Prague, Czech Republic
2 Laboratory of Cell Signalization, Institute of Microbiology of the CAS, v.v.i., Vídeňská 1083, 14220 Prague, Czech Republic

Special Issues: Environmental applications of Biofilms

The work is focused on spontaneous colonization of fungal mycelium by invading microorganisms in a trickle-bed fungal bioreactor operating under semi-sterile conditions. Pleurotus ostreatus was grown under the flow of synthetic wastewater containing activated sludge bacteria and the microbial consortium developed in the reactor was characterized. Genotype and phenotype profile of the reactor-invading, bacterial consortium was clearly distinctive from that of the original activated sludge. The bacterial consortium from the reactor contained a higher portion of bacteria capable of cellobiose utilization and a small amount of bacteria with the ability to utilize benzoic acids. The invading bacteria had no effect on the dye decolorization performance of the fungal reactor. Five bacterial strains colonizing P. ostreatus reactor cultures were isolated and identified as species of the genera Pseudomonas and Bacillus. Except for Bacillus cereus all strains displayed a potential to inhibit fungal growth on solid media (14 to 51 % inhibition) which was comparable or higher than that of the reference bacterial strains. The pH- and media composition-dependence of the growth inhibition was demonstrated.
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Keywords Pleurotus ostreatus; activated sludge; fungal/bacterial co-culture; trickle-bed bioreactor; dye decolorization capacity; microbial community structure; fungal growth inhibition

Citation: Kateřina Svobodová, Denisa Petráčková, Hana Szabad, Čeněk Novotný. Selective adhesion of wastewater bacteria to Pleurotus ostreatus mycelium in a trickle-bed bioreactor. AIMS Environmental Science, 2016, 3(3): 395-407. doi: 10.3934/environsci.2016.3.395


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Copyright Info: 2016, Čeněk Novotný, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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