Research article

An alkali-tolerant strain Microbacterium esteraromaticum C26 produces a high yield of cation-independent bioflocculant

  • Received: 05 May 2016 Accepted: 02 July 2016 Published: 18 July 2016
  • A novel cation-independent bioflocculant BF-C26 produced by an alkali-tolerant strain Microbacterium esteraromaticum C26 was investigated in this study. The fermentation conditions for BF-C26 production were analyzed, including initial pH, carbon source, nitrogen source and the ratio of carbon source to nitrogen source. The result showed that 4.92 g/L bioflocculant BF-C26 was obtained at the following optimized conditions: 3 g/L glucose as carbon source, 10 g/L peptone as nitrogen source and initial pH 8.1. The component assay showed that BF-C26 was a complex of 52.8% proteins and 28.1% polysaccharides. The effects of physicochemical parameters including metal ions, temperature, pH and bioflocculant dosage on flocculating properties of BF-C26 were optimized. The highest flocculating rate of 94.82% was achieved when 4 mg/L BF-C26 was added into the kaolin suspension at pH 8.23 and 30 °C. In this study, Microbacterium esteraromaticum was reported for the first time as a cation-independent bioflocculant producing strain.

    Citation: Weijie Liu, Rongnan He, Cong Liu. An alkali-tolerant strain Microbacterium esteraromaticum C26 produces a high yield of cation-independent bioflocculant[J]. AIMS Environmental Science, 2016, 3(3): 408-419. doi: 10.3934/environsci.2016.3.408

    Related Papers:

  • A novel cation-independent bioflocculant BF-C26 produced by an alkali-tolerant strain Microbacterium esteraromaticum C26 was investigated in this study. The fermentation conditions for BF-C26 production were analyzed, including initial pH, carbon source, nitrogen source and the ratio of carbon source to nitrogen source. The result showed that 4.92 g/L bioflocculant BF-C26 was obtained at the following optimized conditions: 3 g/L glucose as carbon source, 10 g/L peptone as nitrogen source and initial pH 8.1. The component assay showed that BF-C26 was a complex of 52.8% proteins and 28.1% polysaccharides. The effects of physicochemical parameters including metal ions, temperature, pH and bioflocculant dosage on flocculating properties of BF-C26 were optimized. The highest flocculating rate of 94.82% was achieved when 4 mg/L BF-C26 was added into the kaolin suspension at pH 8.23 and 30 °C. In this study, Microbacterium esteraromaticum was reported for the first time as a cation-independent bioflocculant producing strain.


    加载中
    [1] Salehizadeh H, Shojaosadati S (2001) Extracellular biopolymeric flocculants: recent trends and biotechnological importance. Biotechnol Adv 19: 371-385. doi: 10.1016/S0734-9750(01)00071-4
    [2] Liu C, Wang K, Jiang JH, et al. (2015) A novel bioflocculant produced by a salt-tolerant, alkaliphilic and biofilm-forming strain Bacillus agaradhaerens C9 and its application in harvesting Chlorella minutissima UTEX2341. Biochem Eng J 93: 166-172. doi: 10.1016/j.bej.2014.10.006
    [3] Salehizadeh H, Yan N (2014) Recent advances in extracellular biopolymer flocculants. Biotechnol Adv 32: 1506-1522. doi: 10.1016/j.biotechadv.2014.10.004
    [4] Wan C, Zhao XQ, Guo SL, et al. (2013) Bioflocculant production from Solibacillus silvestris W01 and its application in cost-effective harvest of marine microalga Nannochloropsis oceanica by flocculation. Bioresour Technol 135: 207-212. doi: 10.1016/j.biortech.2012.10.004
    [5] Crapper DR, Krishnan SS, Dalton AJ (1973) Brain aluminum distribution in Alzheimer's disease and experimental neurofibrillary degeneration. Science 180: 511-513. doi: 10.1126/science.180.4085.511
    [6] Xia SQ, Zhang ZQ, Wang XJ, et al. (2008) Production and characterization of a bioflocculant by Proteus mirabilis TJ-1. Bioresour Technol 99: 6520-6527. doi: 10.1016/j.biortech.2007.11.031
    [7] Zheng Y, Ye ZL, Fang XL, et al. (2008) Production and characteristics of a bioflocculant produced by Bacillus sp. F19. Bioresour Technol 99: 7686-7691. doi: 10.1016/j.biortech.2008.01.068
    [8] Xiong Y, Wang Y, Yu Y, et al. (2010) Production and characterization of a novel bioflocculant from Bacillus licheniformis. Appl Environ Microbiol 76: 2778-2782. doi: 10.1128/AEM.02558-09
    [9] Liu WJ, Wang K, Li BZ, et al. (2010) Production and characterization of an intracellular bioflocculant by Chryseobacterium daeguense W6 cultured in low nutrition medium. Bioresour Technol 101: 1044-1048. doi: 10.1016/j.biortech.2009.08.108
    [10] Zhong C, Xu A, Chen L, et al. (2014) Production of a bioflocculant from chromotropic acid waste water and its application in steroid estrogen removal. Colloids Surf B: Biointerfaces 122: 729-737. doi: 10.1016/j.colsurfb.2014.08.006
    [11] Liu WJ, Yuan HL, Yang JS, et al. (2009) Characterization of bioflocculants from biologically aerated filter backwashed sludge and its application in dying wastewater treatment. Bioresour Technol 100: 2629-2632. doi: 10.1016/j.biortech.2008.12.017
    [12] He N, Li Y, Chen J, et al. (2004) Production of a novel polygalacturonic acid bioflocculant REA-11 by Corynebacterium glutamicum. Bioresour Technol 94: 99-105. doi: 10.1016/j.biortech.2003.11.013
    [13] Wang SG, Gong WX, Liu XW, et al. (2007) Production of a novel bioflocculant by culture of Klebsiella mobilis using dairy wastewater. Biochem Eng J 36: 81-86. doi: 10.1016/j.bej.2007.02.003
    [14] Yang YN, Ren N, Xue JM, et al. (2007) Mutation effect of MeV protons on bioflocculant bacteria Bacillus cereus. Nucl Instrum Methods Phys Res Sec B 262: 220-224. doi: 10.1016/j.nimb.2007.05.016
    [15] Guo J, Lau AK, Zhang Y, et al. (2015) Characterization and flocculation mechanism of a bioflocculant from potato starch wastewater. Appl Microbiol Biotechnol 99: 5855-5861. doi: 10.1007/s00253-015-6589-y
    [16] Pu S, Qin L, Che J, et al. (2014) Preparation and application of a novel bioflocculant by two strains of Rhizopus sp. using potato starch wastewater as nutrilite. Bioresour Technol 162: 184-191.
    [17] Wong YS, Ong SA, Teng TT, et al. (2012) Production of bioflocculant by Staphylococcus cohnii ssp. from palm oil mill effluent (POME). Water Air Soil Poll 223: 3775-3781.
    [18] Aljuboori AHR, Uemura Y, Osman NB, et al. (2014) Production of a bioflocculant from Aspergillus niger using palm oil mill effluent as carbon source. Bioresour Technol 171: 66-70. doi: 10.1016/j.biortech.2014.08.038
    [19] Zhang ZQ, Lin B, Xia SQ, et al. (2007) Production and application of a novel bioflocculant by multiple-microorganism consortia using brewery wastewater as carbon source. J Environ Sci 19: 667-673. doi: 10.1016/S1001-0742(07)60112-0
    [20] Guo JY, Yu J, Xin X, et al. (2015) Characterization and flocculation mechanism of a bioflocculant from hydrolyzate of rice stover. Bioresour Technol 177: 393-397. doi: 10.1016/j.biortech.2014.11.066
    [21] Wang L, Ma F, Lee DJ, et al. (2013) Bioflocculants from hydrolysates of corn stover using isolated strain Ochrobactium ciceri W2. Bioresour Technol 145: 259-263. doi: 10.1016/j.biortech.2012.11.020
    [22] Liu, WJ, Zhao CC, Jiang JH, et al. (2015) Bioflocculant production from untreated corn stover using Cellulosimicrobium cellulans L804 isolate and its application to harvesting microalgae. Biotechnol Biofuels 8: 170.
    [23] Li Z, Chen RW, Lei HY, et al. (2009) Characterization and flocculating properties of a novel bioflocculant produced by Bacillus circulans. World J Microb Biotechnol 25: 745-752. doi: 10.1007/s11274-008-9943-8
    [24] Li Z, Zhong S, Lei HY, et al. (2009) Production of a novel bioflocculant by Bacillus licheniformis X14 and its application to low temperature drinking water treatment. Bioresour Technol 100: 3650-3656. doi: 10.1016/j.biortech.2009.02.029
    [25] Gong WX, Wang SG, Sun XF, et al. (2008) Bioflocculant production by culture of Serratia ficaria and its application in wastewater treatment. Bioresour Technol 99: 4668-4674. doi: 10.1016/j.biortech.2007.09.077
    [26] He J, Zou J, Shao Z, et al. (2010) Characteristics and flocculating mechanism of a novel bioflocculant HBF-3 produced by deep-sea bacterium mutant Halomonas sp. V3a’. World J Microb Biotechnol 26: 1135-1141. doi: 10.1007/s11274-009-0281-2
    [27] Gao J, Bao H, Xin M, et al. (2006) Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31. J Zhejiang Univ Sci B 7: 186-192. doi: 10.1631/jzus.2006.B0186
    [28] Okaiyeto K, Nwodo UU, Mabinya LV, et al. (2015) Bacillus toyonensis strain AEMREG6, a bacterium isolated from south African marine environment sediment samples produces a glycoprotein bioflocculant. Molecules 20: 5239-5259. doi: 10.3390/molecules20035239
    [29] Yim JH, Kim SJ, Ahn SH, et al. (2007) Characterization of a novel bioflocculant, p-KG03, from a marine dinoflagellate, Gyrodinium impudicum KG03. Bioresour Technol 98: 361-367. doi: 10.1016/j.biortech.2005.12.021
    [30] Aljuboori AHR, Idris A, Abdullah N, et al. (2013) Production and characterization of a bioflocculant produced by Aspergillus flavus. Bioresour Technol 127: 489-493.
    [31] Tang W, Song L, Li D, et al. (2014) Production, characterization, and flocculation mechanism of cation independent, pH tolerant, and thermally stable bioflocculant from Enterobacter sp. ETH-2. PloS one 9: e114591. doi: 10.1371/journal.pone.0114591
    [32] Aljuboori AHR, Idris A, AI-joubory HHR, et al. (2015) Flocculation behavior and mechanism of bioflocculant produced by Aspergillus flavus. J Environ Manage 150: 466-471. doi: 10.1016/j.jenvman.2014.12.035
    [33] Liu WJ, Liu C, Yuan HL, et al. (2015) The mechanism of kaolin clay flocculation by a cation-independent bioflocculant produced by Chryseobacterium daeguense W6. AIMS Environ Sci 2: 169-179. doi: 10.3934/environsci.2015.2.169
    [34] Chaplin MF, Kennedy JF (1994) Carbohydrate analysis: a practical approach. IRL Press Ltd.
    [35] Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254. doi: 10.1016/0003-2697(76)90527-3
    [36] Malik A, Sakamoto M, Ono T, et al. (2003) Coaggregation between Acinetobacter johnsonii S35 and Microbacterium esteraromaticum strains isolated from sewage activated sludge. J Biosci Bioeng 96: 10-15. doi: 10.1016/S1389-1723(03)90090-9
    [37] Lu WY, Zhang T, Zhang DY, et al. (2005) A novel bioflocculant produced by Enterobacter aerogenes and its use in defecating the trona suspension. Biochem Eng J 27: 1-7.
    [38] Deng S, Yu G, Ting YP (2005) Production of a bioflocculant by Aspergillus parasiticus and its application in dye removal. Colloids Surf B: Biointerfaces 44: 179-186. doi: 10.1016/j.colsurfb.2005.06.011
    [39] Shih IL, Van YT, Yeh LC, et al. (2001) Production of a biopolymer flocculant from Bacillus licheniformis and its flocculation properties. Bioresour Technol 78: 267-272. doi: 10.1016/S0960-8524(01)00027-X
    [40] Luo Z, Chen L, Chen C, et al. (2014) Production and characteristics of a bioflocculant by Klebsiella pneumoniae YZ-6 isolated from human saliva. Appl Biochem Biotechnol 172: 1282-1292. doi: 10.1007/s12010-013-0601-8
    [41] Zhao HJ, Liu HT, Zhou JG (2013) Characterization of a bioflocculant MBF-5 by Klebsiella pneumoniae and its application in Acanthamoeba cysts removal. Bioresour Technol 137: 226-232. doi: 10.1016/j.biortech.2013.03.079
    [42] Gomaa EZ (2012) Production and characteristics of a heavy metals removing bioflocculant produced by Pseudomonas aeruginosa. Pol J Microbiol 61: 281-289.
    [43] Okaiyeto K, Nwodo UU, Mabinya LV, et al. (2013) Characterization of a bioflocculant produced by a consortium of Halomonas sp. Okoh and Micrococcus sp. Leo. Int J Environ Res Public Health 10: 5097-5110. doi: 10.3390/ijerph10105097
  • Reader Comments
  • © 2016 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Metrics

Article views(2289) PDF downloads(1025) Cited by(3)

Article outline

Figures and Tables

Figures(4)  /  Tables(1)

Other Articles By Authors

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return

Catalog