Effective elimination of water-borne <em>Escherichia coli </em>using archaeal poly-<span style="font-family: Symbol">g</span>-glutamate-based materials

Yuichi Hakumai; Kanae Yamaguchi; Sawami Nakayama; Makoto Ashiuchi; Yuichi Hakumai; Kanae Yamaguchi; Sawami Nakayama; Makoto Ashiuchi

doi:10.3934/microbiol.2016.3.222

AIMS Microbiology

2016, Volume 2, Issue 3: 222-229. doi: 10.3934/microbiol.2016.3.222

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Communication

Effective elimination of water-borne Escherichia coli using archaeal poly-g-glutamate-based materials

1.
Course of Applied Bioresource Science, United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan
2.
Faculty of Agriculture, Kochi University, Nankoku, Kochi 783-8502, Japan

Received: 27 April 2016 Accepted: 05 July 2016 Published: 06 July 2016

Escherichia coli is used worldwide as a fecal indicator species to assess the quality of (drinking) water. Active carbons are used for the removal of chemical pollutants, but are ineffective in the inactivation of water-borne pathogens such as E. coli. Herein, we developed poly-g-glutamate-ion complex-coated active carbons (PGAIC-AC) and examined their ability to eliminate E. coli from a laboratory model of water pollution (~ 2.0 × 10⁴ CFU/mL) at room temperature. The results showed that E. coli was virtually eliminated when using PGAIC-AC as a dispersant. In fact, the log reduction values were estimated to be > 1.19. In this study, we further constructed simple but effective bacteria-elimination system with a PGAIC-AC–embedded column. This PGAIC-AC system can be utilized to purify water when no electricity or specialized equipment is available.
- bacteria elimination,
- water,
- purification,
- poly-γ-glutamate,
- active carbons
Citation: Yuichi Hakumai, Kanae Yamaguchi, Sawami Nakayama, Makoto Ashiuchi. Effective elimination of water-borne Escherichia coli using archaeal poly-g-glutamate-based materials[J]. AIMS Microbiology, 2016, 2(3): 222-229. doi: 10.3934/microbiol.2016.3.222

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Abstract

Escherichia coli is used worldwide as a fecal indicator species to assess the quality of (drinking) water. Active carbons are used for the removal of chemical pollutants, but are ineffective in the inactivation of water-borne pathogens such as E. coli. Herein, we developed poly-g-glutamate-ion complex-coated active carbons (PGAIC-AC) and examined their ability to eliminate E. coli from a laboratory model of water pollution (~ 2.0 × 10⁴ CFU/mL) at room temperature. The results showed that E. coli was virtually eliminated when using PGAIC-AC as a dispersant. In fact, the log reduction values were estimated to be > 1.19. In this study, we further constructed simple but effective bacteria-elimination system with a PGAIC-AC–embedded column. This PGAIC-AC system can be utilized to purify water when no electricity or specialized equipment is available.

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