Review

A review on bioremediation by microbial immobilization-an effective alternative for wastewater treatment

  • In this review, we describe recent developments and strategies involved in the utilization of solid supports for the management of wastewater by means of biological treatments. The origin of wastewater determines whether it is considered natural or industrial waste, and the source(s) singly or collectively contribute to increase water pollution. Pollution is a threat to aquatic and humans; thus, before the discharge of treated waters back into the environment, wastewater is put through a number of treatment processes to ensure its safety for human use. Biological treatment or bioremediation has become increasingly popular due to its positive impact on the ecosystem, high level of productivity, and process application cost-effectiveness. Bioremediation involving the use of microbial cell immobilization has demonstrated enhanced effectiveness compared to free cells. This constitutes a significant departure from traditional bioremediation practices (entrapment, adsorption, encapsulation), in addition to its ability to engage in covalent bonding and cross-linking. Thus, we took a comparative look at the existing and emerging immobilization methods and the related challenges, focusing on the future. Furthermore, our work stands out by highlighting emerging state-of-the-art tools that are bioinspired [enzymes, reactive permeable barriers linked to electrokinetic, magnetic cross-linked enzyme aggregates (CLEAs), bio-coated films, microbiocenosis], as well as the use of nanosized biochar and engineered cells or their bioproducts targeted at enhancing the removal efficiency of metals, carbonates, organic matter, and other toxicants and pollutants. The potential integration of 'omics' technologies for enhancing and revealing new insights into bioremediation via cell immobilization is also discussed.

    Citation: Frank Abimbola Ogundolie, Olorunfemi Oyewole Babalola, Charles Oluwaseun Adetunji, Christiana Eleojo Aruwa, Jacqueline Njikam Manjia, Taoheed Kolawole Muftaudeen. A review on bioremediation by microbial immobilization-an effective alternative for wastewater treatment[J]. AIMS Environmental Science, 2024, 11(6): 918-939. doi: 10.3934/environsci.2024046

    Related Papers:

    [1] Kun Wang, Lin Mu . Numerical investigation of a new cut finite element method for Stokes interface equations. Electronic Research Archive, 2025, 33(4): 2503-2524. doi: 10.3934/era.2025111
    [2] Bin Wang, Lin Mu . Viscosity robust weak Galerkin finite element methods for Stokes problems. Electronic Research Archive, 2021, 29(1): 1881-1895. doi: 10.3934/era.2020096
    [3] Yan Yang, Xiu Ye, Shangyou Zhang . A pressure-robust stabilizer-free WG finite element method for the Stokes equations on simplicial grids. Electronic Research Archive, 2024, 32(5): 3413-3432. doi: 10.3934/era.2024158
    [4] Derrick Jones, Xu Zhang . A conforming-nonconforming mixed immersed finite element method for unsteady Stokes equations with moving interfaces. Electronic Research Archive, 2021, 29(5): 3171-3191. doi: 10.3934/era.2021032
    [5] Nisachon Kumankat, Kanognudge Wuttanachamsri . Well-posedness of generalized Stokes-Brinkman equations modeling moving solid phases. Electronic Research Archive, 2023, 31(3): 1641-1661. doi: 10.3934/era.2023085
    [6] Jiwei Jia, Young-Ju Lee, Yue Feng, Zichan Wang, Zhongshu Zhao . Hybridized weak Galerkin finite element methods for Brinkman equations. Electronic Research Archive, 2021, 29(3): 2489-2516. doi: 10.3934/era.2020126
    [7] Xiu Ye, Shangyou Zhang . A stabilizer free WG method for the Stokes equations with order two superconvergence on polytopal mesh. Electronic Research Archive, 2021, 29(6): 3609-3627. doi: 10.3934/era.2021053
    [8] Guoliang Ju, Can Chen, Rongliang Chen, Jingzhi Li, Kaitai Li, Shaohui Zhang . Numerical simulation for 3D flow in flow channel of aeroengine turbine fan based on dimension splitting method. Electronic Research Archive, 2020, 28(2): 837-851. doi: 10.3934/era.2020043
    [9] Wenya Qi, Padmanabhan Seshaiyer, Junping Wang . A four-field mixed finite element method for Biot's consolidation problems. Electronic Research Archive, 2021, 29(3): 2517-2532. doi: 10.3934/era.2020127
    [10] Shan Jiang, Li Liang, Meiling Sun, Fang Su . Uniform high-order convergence of multiscale finite element computation on a graded recursion for singular perturbation. Electronic Research Archive, 2020, 28(2): 935-949. doi: 10.3934/era.2020049
  • In this review, we describe recent developments and strategies involved in the utilization of solid supports for the management of wastewater by means of biological treatments. The origin of wastewater determines whether it is considered natural or industrial waste, and the source(s) singly or collectively contribute to increase water pollution. Pollution is a threat to aquatic and humans; thus, before the discharge of treated waters back into the environment, wastewater is put through a number of treatment processes to ensure its safety for human use. Biological treatment or bioremediation has become increasingly popular due to its positive impact on the ecosystem, high level of productivity, and process application cost-effectiveness. Bioremediation involving the use of microbial cell immobilization has demonstrated enhanced effectiveness compared to free cells. This constitutes a significant departure from traditional bioremediation practices (entrapment, adsorption, encapsulation), in addition to its ability to engage in covalent bonding and cross-linking. Thus, we took a comparative look at the existing and emerging immobilization methods and the related challenges, focusing on the future. Furthermore, our work stands out by highlighting emerging state-of-the-art tools that are bioinspired [enzymes, reactive permeable barriers linked to electrokinetic, magnetic cross-linked enzyme aggregates (CLEAs), bio-coated films, microbiocenosis], as well as the use of nanosized biochar and engineered cells or their bioproducts targeted at enhancing the removal efficiency of metals, carbonates, organic matter, and other toxicants and pollutants. The potential integration of 'omics' technologies for enhancing and revealing new insights into bioremediation via cell immobilization is also discussed.



    The viscous Stokes problem seeks unknown functions u and p that fulfill the following equations,

    νΔu+p=finΩ, (1.1)
    u=0inΩ, (1.2)
    u=gonΩ, (1.3)

    where Ω is a polygonal domain in R2. For the nonhomogeneous boundary condition u=gonΩ, one can use the standard procedure by letting u=u0+ug. ug is a known function satisfying ug=g on Ω and u0 is zero at Ω and satisfies (1.1) and (1.2) with different righthand sides. For the sake of simplicity, we only consider the homogeneous boundary condition, i.e., g=0. The scheme can be extended to the nonhomogeneous boundary condition. Using the standard notation for the Sobolev spaces, the weak formulation for the Stokes problems (1.1)–(1.3), in the primary velocity-pressure form, we seek u[H10(Ω)]2 and pL20(Ω) such that,

    {(νu,v)(v,p)=(f,v), v[H10(Ω)]2,(u,q)=0,   qL20(Ω).

    In the standard finite element discretization schemes, pressure and velocity unknowns are approximated simultaneously via a saddle-point system. To avoid solving such an indefinite system, the divergence-free finite element methods have been proposed to compute the numerical velocity by solving a symmetric positive-definite system in a divergence-free subspace. Due to the discrete or exact divergence-free property, such a method eliminates the pressure from the coupled systems, resulting in a symmetric positive definite system with a smaller size. Previously, a divergence-free basis was constructed for different finite element methods, e.g., [1,2,3,4]. The original divergence-free weak Galerkin (WG) method was proposed in [3].

    Unlike most existing divergence-free finite element methods, the discrete divergence-free WG method considered in this paper allows the meshes to consist of a mix of general polygons and hanging nodes. However, although the basis functions are discrete divergence-free, they may not guarantee good velocity approximation since the velocity error may depend on viscosity and pressure. This is because the div-free scheme is non-pressure-robust; thus, the velocity error bound depends on viscosity and pressure. Small viscosity values or inaccurate pressure approximations may produce an incorrect velocity solution to ruin the simulation.

    This paper shows that the numerical pollution mentioned above, caused by small viscosities or large pressure errors, also appears for the previous discrete divergence-free WG method. In this paper, we contribute to modify the original scheme and investigate the technique to remove viscosity and pressure effects in the velocity approximations with minimal effort. The technique follows the previous work of the authors and employs the velocity-reconstruction operator to modify the load term. This reconstruction technique was first proposed by Linke [5,6] and was then widely used to modify the existing finite element scheme for Stokes problems [7,8,9,10,11,12,13,14,15,16] and other incompressible fluid problems due to the minimal efforts required to achieve the desired good quality in numerical solutions [17,18,19,20,21,22]. Unlike using the H(div) basis functions in H(div) finite element methods, the velocity reconstruction operator is designed to map the original velocity basis functions to a suitable subspace of the H(div) space. Then, this modification only changes the load term assembly, but the stiffness matrix remains the same. In addition to the velocity reconstruction operator, we also mention that there are other advanced approaches to achieve the desired pressure-robustness [17,23,24]. Due to the page limitation, we only cite an incomplete list of the previous schemes featuring pressure-robustness. For example, Zhang [25,26] constructed divergence-free pairs of finite element spaces and used it to solve incompressible fluid problems [27,28]. Another successful strategy is to employ the Stokes complex of the lowest regularity [29] and the approximate velocity in the H(div) space [30]. A similar approach has been used in hybrid discontinuous Galerkin methods (HDG) to achieve the desired pressure-robustness [31,32,33]. More details on divergence-free and pressure-robust schemes can be found in the review paper [34]. Recently, there is another approach to achieve the desired robustness by enriching the Raviart-Thomas (RT) basis functions into the H1 -finite element spaces [35,36]. In this paper, we focus on designing the proper velocity-reconstruction operator and modifying the source term assembly to achieve robustness. The advantages of this modification lie in the potential to recycle the researchers' previous codes and enhance the former work with minimal changes to the reliable numerical approximation. We also demonstrate the pressure recovery procedure for the case that requires a pressure approximation.

    The rest of this paper is organized as follows. In Section 2, we first introduce the notation and two existing numerical algorithms and then propose the robust pressure algorithm and the pressure recovery scheme. In Section 3, we demonstrate the main error estimates for the Stokes problem. Several numerical experiments are presented in Section 4. We conclude this paper in Section 5.

    This section recalls the standard WG method and proposes our new divergence-free and pressure-robust WG methods. Let Th be a partition of the domain Ω consisting of a mix of polygons satisfying the set of conditions specified in [37]. Let Eh denote the set of all edges in Th and E0h=EhΩ be the set of all interior edges. Based on the partition Th, we introduce the following finite element spaces Wh and Vh for the pressure and velocity variables, respectively,

    Wh={q: qL20(Ω), q|TP0(T)},Vh={v={v0,vb}: {v0,vb}|T[P1(T)]2×[P0(e)]2, eT, vb=0 on Ω},

    where Pk(ω) denotes the space of polynomials of degree at most k restricted to ω=e or T.

    The discrete weak gradient and divergence operators are defined locally on each TTh as follows.

    Definition 2.1. The discrete weak gradient w:Vh[P0(T)]2×2 and weak divergence operator w:VhP0(T) are defined as follows,

    (wv,q)T=vb,qnT,q[P0(T)]2×2,(wv,φ)T=vbn,φT,φP0(T).

    For each edge eEh, let Qb be the L2 projection from [L2(e)]2 onto [P0(e)]2. Then, we define

    a(v,w):=TTh(νwv, ww)T+TThνhTQbv0vb,Qbw0wbT,b(v,q):=TTh(wv, q)T.

    Here, hT denotes the mesh size for the element T. Then, a standard WG algorithm (see [38]) is as follows.

    Algorithm 2.1. Standard weak Galerkin algorithm (SWG) A numerical approximation for (1.1)–(1.3) is to seek uh={u0,ub}Vh and phWh such that

    a(uh,v)b(v,ph)=(f,v0), v={v0,vb}Vh,b(uh,q)=0,    qWh.

    Algorithm 2.1 produces a saddle system, which can be challenging due to its indefiniteness, strong coupling between velocity and pressure, and large size. In some cases, linear solvers for this large system involving both velocity and pressure may not be effective. Instead, we use the divergence-free basis to decouple the velocity and pressure and solve a smaller system, which is symmetric positive definite.

    In this section, we introduce the divergence-free basis. First, we define the discrete divergence-free subspace Dh of Vh in the usual way (see [1,2,3]) as follows,

    Dh={vVh;b(v,q)=0,qWh}. (2.1)

    Following the techniques introduced in [3] and using the definition (2.1), we explicitly construct the basis functions as the following three types.

    Dh=span{Φ1,,Φ6NKΨ0,Υ1,,ΥNEΨt,Λ1,,ΛNVΨV}. (2.2)

    1) Type 1 (Ψ0): For each TiTh, i=1,,NK with NK being the number of elements, all the six linearly independent linear functions Φ6(i1)+1,Φ6(i1)+2,,Φ6(i1)+6 in Vh are discrete divergence-free since they are nonzero only in the interior of element Ti.

    2) Type 2 (Ψt): For each eiE0h, i=1,,NE with NE being the number of interior edges, let tei be its tangential vector and Ψei,1 and Ψei,2 be the two basis functions of Vh that are nonzero only on ei. Define Υi:=C1Ψei,1+C2Ψei,2 such that Υi|ei=tei. It is easy to verify that Υi is discrete divergence-free using the divergence theorem. Note that Υi is nonzero only on ei.

    3) Type 3 (ΨV): For each interior vertex PiVh, i=1,,NV with NV being the number of interior vertices, there are r elements sharing Pi which form a hull HPi as shown in Figure 1. Consequently, there are r interior edges ej (j=1,,r) incident with Pi. Let nej be a normal vector on ej, and we assume that the normal vectors nej j=1,,r are counterclockwise around the vertex Pi as shown in Figure 1. For each ej, let Ψej,1 and Ψej,2 be the two basis functions of Vh, which are nonzero only on ej. Define Θj=C1Ψej,1+C2Ψej,2Vh such that Θj|ej=nej and then define Λi=rj=11|ej|Θj, which is discrete divergence-free by the divergence theorem. The construction can be applied to triangular and polygonal grids, as shown in Figure 1.

    Figure 1.  Hull HPi for triangular grids and polygonal grids.

    The dimension of Vh is 6NK+2NE. Since we use a piecewise constant space Wh for the pressure, there are NK1 divergence-free constraints. Subtracting the number of divergence-free constraints from the total degrees of freedom (DoFs), (6NK+2NE)(NK1)=6NK+NE+NV (where we use the Euler's formula in 2D), we get the dimension of the discrete divergence-free subspace Dh. Note that the total number of the three types of divergence-free basis functions is exactly 6NK+NE+NV, indicating that we found all the basis functions that are supported locally. Specifically, the above basis functions correspond to the components of u0 and ub={ut,uV}. The basis functions for u0, i.e., {Ψ0}, are defined only on the interior of each element T, which is the same as the previous SWG element. The basis functions for ut, that is, {Ψt}, are defined only on each edge eE0h along the tangential direction, and the basis functions for uV, i.e., {ΨV}, are defined only on the edges incident with the vertex V.

    Using the divergence-free basis (2.2), the decoupled algorithm can be proposed to solely solve the velocity uh as follows; see [3] for more details.

    Algorithm 2.2. Divergence-free WG algorithm A discrete divergence free approximation for (1.1)–(1.3) is to find uh={u0,ub}Dh such that

    a(uh, v)=(f,v0),v={v0,vb}Dh.

    Although this algorithm decouples the unknown variables in u and p, it is essentially equivalent to the SWG Algorithm 2.1. Thus, the velocity error still depends on the pressure error (see Theorem 1 and Table 2). This may cause inaccuracy and instability when problems occur with a low viscosity and a pressure singularity. This computational challenge can be resolved using the pressure-robust enhancement, which will be discussed in the next section.

    We shall employ the velocity reconstruction operator to enhance Algorithm 2.2. The reconstruction operator Πhv:Dh˜DhH(div;Ω) is defined as

    eΠhvnds=evnds. (2.3)

    Let ˜Dh|T=RT0(T)H(div;Ω). As the fact that Ψt is aligning the tangential direction on each edge, we only need to compute the reconstruction operator corresponding to ΨV={Λ1ΛNV}. It gives

    Πhv={0, if v=v0Ψ0,0, if v=vbΨt,ΠhΛj, if v=vbΨV=span{Λi,i=1,,NV}.

    Here, it is easy to verify that in (2.3): ΠhΛi=rj=1signejϕRT0ej, where ϕRT0ej is the corresponding RT0 basis on the edge e. We associate a unit normal vector ne with eE0h, which is assumed to be oriented from T+ to T. If e is a boundary edge/face, then ne is the unit outward normal vector to Ω. For the outer normal n, if [n|T]ej=nej, we assign signej=1; if [n|T]ej=nej, we assign signej=1. Thus, by employing Πh, we propose the following pressure-robust scheme.

    Algorithm 2.3. Pressure-robust divergence-free WG algorithm A pressure-robust divergence-free approximation for (1.1)–(1.3) is to find uh={u0,ub}Dh satisfying

    a(uh,v)=(f,Πhv),v={v0,vb}Dh.

    As we can see from the discretization, the stiffness matrix is the same as Algorithm 2.2, but only the load vector changes. By this minor modification, the desired pressure-robustness can be achieved. The results will be demonstrated in Theorem 2 and validated in numerical experiments.

    Remark 2.1. For triangular, rectangular, tetrahedral, and cubic meshes, we can directly employ the associated RT0 or RT[0] basis functions to perform the velocity reconstruction. For polygonal / polyhedral meshes, the techniques in [7,8] can be used to build the operator Πhv.

    In Algorithms 2.2 and 2.3, we decouple the unknowns and only compute the velocity solution uh. In some cases, the pressure variable is also needed. In this section, we propose the following procedure that computes the pressure after obtaining the velocity uh.

    Algorithm 2.4. Pressure recovering algorithm The pressure can be obtained by solving the following equation: find phWh such that

    b(v,ph)=(v)a(uh,v), vVhDh.

    Here, (v)=(f,v0) for Algorithm 2.2 and (v)=(f,Πhv) for Algorithm 2.3. As vVhDh, let us assume ph=ph|T is already known, and we can choose v={v0=0,vb=ne} with eT and the value of p+h=ph on the adjacent element sharing the edge e is not computed. Then, the definition of b(,) implies b(v,ph)=(wv,ph)=Tvbne,phT=vbne,[[ph]]e=|e|(p+hph). Here, ne denotes the normal direction from the current element T to its adjacent element that shares the edge e. In the implementation, we can assume ph|T1=0 to start and compute all the values in ph|T as above sequentially and locally. There is no need to form the global matrix explicitly.

    In the above proposed algorithms, we can do further DoFs enhancement by eliminating the unknowns corresponding to u0 to obtain a smaller system. This elimination can be done locally when the global matrix is assembled via static condensation. To state the local elimination procedure, denote by Dh(T) the restriction of Dh on T, i.e.,

    Dh(T)={v={v0,vb}Dh,v(x)=0, for xT}.

    Algorithm 2.5. An approximation to the problem (1.1)–(1.3) is given by seeking uh={u0,ut,ub}Dh satisfying a global equation

    a(uh,v)=0, v={0,Ψt,Ψb}Dh,

    and a local system on each element TTh,

    a(uh,v)=(f,v0), v={Ψ0,0,0}Dh(T).

    Remark 2.2. The above algorithm consists of a local system solved on each element TTh to eliminate u0 and a global system for ub, and a global system has ub as its only unknowns that will reduce the number of unknowns of the WG system. The comparison of DoFs is shown in Figure 2.

    Figure 2.  Sparsity pattern for Algorithm 2.2/2.3 (left) and Algorithm 2.5 (right) for uniform mesh with h=1/16.

    In this section, we present the error analysis of Algorithm 2.3 to demonstrate its advantages. Denote by Q0 the L2 projection operator from [L2(T)]2 onto [P1(T)]2. Define Qhu={Q0u,Qbu}Vh and let Qhp be the local L2 projections onto P0(T). Furthermore, we define the following norm corresponding to {the} WG finite element methods:

    |||v|||:=(TTh(wv2T+h1TQbv0vb2T))1/2.

    The following optimal error estimates have been derived in [3,38].

    Theorem 3.1. (Non-pressure robust scheme) Let (u;p)[H10(Ω)H2(Ω)]2×(L20(Ω)H1(Ω)) be the solution of (1.1)–(1.3) and (uh;ph)Vh×Wh be the solutions of (1.1)–(1.3) and Algorithm 2.1 or Algorithms 2.2–2.4, respectively. Then, the following error estimate holds true,

    |||Qhuuh|||Ch(u2+1νp1),QhpphCh(νu2+p1). (3.1)

    Proof. The proofs can be found in [3].

    Theorem 3.2. (Pressure-robust scheme) Let (u;p)[H10(Ω)H2(Ω)]2×(L20(Ω)H1(Ω)) be the solution of (1.1)–(1.3) and (uh;ph)Vh×Wh be the solution of (1.1)–(1.3) and Algorithms 2.3 and 2.4, respectively. Then, the following error estimate holds true,

    |||Qhuuh|||Chu2,QhpphChνu2. (3.2)

    Proof. By estimating the inconsistent errors caused by changing the righthand load vector and following the techniques in [3], the theorem can be proved.

    Remark 3.1. Although the divergence-free scheme only needs to solve the velocity component, the velocity error may still depend on the pressure, which is a non-pressure-robust scheme as shown in Theorem 1. By modifying the load vector, we completely remove the pressure dependence in the error estimate to achieve the desired pressure robustness. Besides, the pressure-robust error analysis can be obtained similarly to the rigorous analysis in [7].

    In this section, we test several benchmark problems to report numerical performance and validate the convergence results shown in Theorems 1 and 2. In all numerical tests, triangular meshes have been used.

    Let Ω=(0,1)×(0,1) and the exact solution u and p be,

    u=(10x2y(x1)2(2y1)(y1)10xy2(2x1)(x1)(y1)2) and p=10(2x1)(2y1).

    Denote the errors e={(Q0uu0,Qbuub)} and ϵ=Qhpph. We first compare the computational cost corresponding to Algorithm 2.1, Algorithm 2.2/2.3, and Algorithm 2.5. Since the stiffness matrix corresponding to Algorithms 2.2 and 2.3 is identical, we only compute the DoFs for Algorithm 2.2. The profiles for DoFs are reported in Table 1. Here, we exclude the unknowns for ub for the Dirichlet boundary as computing the required DoFs.

    Table 1.  Example 4.1: Comparison of DoFs for different algorithms. Here, "DoFs" denotes the degrees of freedom and "nnz" denotes the number of nonzeros of the stiffness matrix.
    Algorithm 2.1 Algorithm 2.2/2.3 Algorithm 2.5
    N DoFsu DoFsp DoFs DoFs nnz DoFs nnz
    4 272 32 304 241 1861 49 360
    8 1120 128 1248 993 8805 225 1984
    16 4544 512 5056 4033 38, 245 961 9168
    32 18, 304 2048 20, 352 16, 257 159, 333 3969 39, 280
    64 73, 472 8192 81, 664 65, 281 650, 341 16, 129 162, 480
    128 294, 400 32, 768 327, 168 261, 633 2, 627, 685 65, 025 660, 784
    256 1, 178, 624 131, 072 1, 309, 696 1, 047, 553 10, 563, 685 261, 121 2, 665, 008

     | Show Table
    DownLoad: CSV

    In this table, we first observe that the required DoFs can be significantly reduced by employing the divergence-free basis. Since we only modify the assembly of the source term in Algorithm 2.3, the required DoFs in Algorithms 2.2 and 2.3 remain the same. As static condensation is employed (Algorithm 2.5), the DoFs of the global system can be further reduced. For example, when N=256, the size of the global system is reduced from 1 M to 0.2 M, while the density of the matrix increased from 1E-5 to 4E-5.

    Next, we will test the performance corresponding to non-pressure-robust scheme, Algorithm 2.2/Algorithm 2.4, and pressure-robust scheme, Algorithm 2.3/Algorithm 2.4, for a sequence of meshes and varying values in ν. In Table 2, we report the error profiles and the convergence results. We observed that:

    Table 2.  Example 4.1: Error profiles and convergence results.
    1/h e0 order e0 order ϵ order e0 order e0 order ϵ order
    Algorithm 2.2/Algorithm 2.4 Algorithm 2.3/Algorithm 2.4
    ν=1
    8 6.30E-2 5.23E-1 1.29E+0 8.27E-3 1.70E-1 2.71E-2
    16 1.72E-2 1.9 2.84E-1 0.9 6.31E-1 1.0 2.17E-3 1.9 8.74E-2 1.0 1.17E-2 1.2
    32 4.47E-3 1.9 1.47E-1 0.9 3.00E-1 1.1 5.50E-4 2.0 4.41E-2 1.0 5.32E-3 1.1
    64 1.13E-3 2.0 7.44E-2 1.0 1.44E-1 1.1 1.38E-4 2.0 2.21E-2 1.0 2.57E-3 1.0
    128 2.84E-4 2.0 3.74E-2 1.0 6.98E-2 1.0 3.46E-5 2.0 1.10E-2 1.0 1.27E-3 1.0
    ν=1E-2
    8 6.21E+0 5.10E+1 1.29E+0 8.27E-3 1.70E-1 2.71E-4
    16 1.70E+0 1.9 2.79E+1 0.9 6.31E-1 1.0 2.17E-3 1.9 8.74E-2 1.0 1.17E-4 1.2
    32 4.41E-1 1.9 1.45E+1 0.9 3.00E-1 1.1 5.50E-4 2.0 4.41E-2 1.0 5.32E-5 1.1
    64 1.12E-1 2.0 7.32E+0 1.0 1.44E-1 1.1 1.38E-4 2.0 2.21E-2 1.0 2.57E-5 1.0
    128 2.80E-2 2.0 3.68E+0 1.0 6.98E-2 1.0 3.46E-5 2.0 1.10E-2 1.0 1.27E-5 1.0
    ν=1E-4
    8 6.21E+2 5.10E+3 1.29E+0 8.27E-3 1.70E-1 2.71E-6
    16 1.70E+2 1.9 2.79E+3 0.9 6.31E-1 1.0 2.17E-3 1.9 8.74E-2 1.0 1.17E-6 1.2
    32 4.41E+1 1.9 1.45E+3 0.9 3.00E-1 1.1 5.50E-4 2.0 4.41E-2 1.0 5.32E-7 1.1
    64 1.12E+1 2.0 7.32E+2 1.0 1.44E-1 1.1 1.38E-4 2.0 2.21E-2 1.0 2.57E-7 1.0
    128 2.80E+0 2.0 3.68E+2 1.0 6.98E-2 1.0 3.46E-5 2.0 1.10E-2 1.0 1.27E-7 1.0
    ν=1E-6
    8 6.21E+4 5.10E+5 1.29E+0 8.27E-3 1.70E-1 2.71E-8
    16 1.70E+4 1.9 2.79E+5 0.9 6.31E-1 1.0 2.17E-3 1.9 8.74E-2 1.0 1.17E-8 1.2
    32 4.41E+3 1.9 1.45E+5 0.9 3.00E-1 1.1 5.50E-4 2.0 4.41E-2 1.0 5.32E-9 1.1
    64 1.12E+3 2.0 7.32E+4 1.0 1.44E-1 1.1 1.38E-4 2.0 2.21E-2 1.0 2.57E-9 1.0
    128 2.80E+2 2.0 3.68E+4 1.0 6.98E-2 1.0 3.46E-5 2.0 1.10E-2 1.0 1.27E-9 1.0
    ν=1E-8
    8 6.21E+6 5.10E+7 1.29E+0 8.27E-3 1.70E-1 2.71E-10
    16 1.70E+6 1.9 2.79E+7 0.9 6.31E-1 1.0 2.17E-3 1.9 8.74E-2 1.0 1.17E-10 1.2
    32 4.41E+5 1.9 1.45E+7 0.9 3.00E-1 1.1 5.50E-4 2.0 4.41E-2 1.0 5.32E-11 1.1
    64 1.12E+5 2.0 7.32E+6 1.0 1.44E-1 1.1 1.38E-4 2.0 2.21E-2 1.0 2.57E-11 1.0
    128 2.80E+4 2.0 3.68E+6 1.0 6.98E-2 1.0 3.46E-5 2.0 1.10E-2 1.0 1.27E-11 1.0

     | Show Table
    DownLoad: CSV

    ● All the error profiles produced by Algorithm 2.2/Algorithm 2.4 and Algorithm 2.3/Algorithm 2.4 result in the optimal convergence rate: the velocity error measured in H1-norm and pressure error measured in L2-norm are of order O(h); the velocity error measured in L2-norm is of order O(h2). The convergence rates stay the same for different viscosity values ν.

    ● The velocity errors produced by the non-pressure-robust scheme Algorithms 2.2–2.4 depend on the viscosity ν. When ν decreases, the velocity error measured in the norms H1- and L2- increases on the order of 1ν. In contrast, the pressure errors measured in L2-norm stay the same as ν varies. These observations agree with (3.1).

    ● The velocity errors produced by pressure-robust scheme Algorithms 2.3 and 2.4 do not depend on the viscosity ν. When ν decreases, the velocity error measured in the norms H1- and L2- remains the same. In contrast, the pressure error measured in L2-norm decreases at the order ν. These observations agree with (3.2).

    ● The above observations validate that, although the scheme can decouple the unknowns in velocity and pressure and solve them independently, the div-free finite element space is sometimes insufficient to ensure the accuracy of numerical solutions with satisfaction.

    In this test, we shall consider the nonhomogeneous Dirichlet boundary conditions. Let Ω=(0,1)×(0,1) and the exact solution is taken as

    u=(sin(πx)sin(πy)cos(πx)cos(πy)), and p=sinx.

    It is easy to see that u|Ω0. Thus, one needs to modify the method in order to deal with nonhomogeneous Dirichlet boundary conditions.

    Table 3 reports the error profiles and convergence results. We compare the performance of Algorithm 2.2/2.4 and Algorithm 2.3/2.4 on a sequence of meshes with different values of ν. To start, non-pressure-robust scheme Algorithm 2.2 and pressure-robust scheme Algorithm 2.3 have been employed to simulate the numerical velocity component. Then, when the velocity approximation is available, Algorithm 2.4 is used to recover the unknown pressure. As in the above test, though Algorithm 2.2 can decouple velocity/pressure and solely solve the unknown velocity, Algorithm 2.2 fails to produce reliable numerical solutions when the viscosity values are small. In contrast, the pressure-robust scheme Algorithm 2.3 is able to produce a viscosity-independent simulation for the velocity. As viscosity values vary, velocity errors (measured in the L2-norm and the H1-norm) remain the same. Furthermore, reducing viscosity values ν produces a more accurate numerical pressure, which gives a convergence rate for the pressure measured in the L2-norm as O(h). These numerical results confirm the theoretical conclusions in the above section.

    Table 3.  Example 4.2: Error profiles and convergence results.
    1/h e0 order e0 order ϵ order e0 order e0 order ϵ order
    Algorithm 2.2/Algorithm 2.4 Algorithm 2.3/Algorithm 2.4
    ν=1
    8 5.12E-2 5.83E-1 4.57E-1 2.81E-2 8.76E-1 7.62E-1
    16 1.28E-2 2.0 2.91E-1 1.0 2.36E-1 1.0 7.69E-3 1.9 4.54E-1 0.9 4.41E-1 0.8
    32 3.18E-3 2.0 1.46E-1 1.0 1.19E-1 1.0 2.00E-3 1.9 2.30E-1 1.0 2.37E-1 0.9
    64 7.95E-4 2.0 7.29E-2 1.0 5.95E-2 1.0 5.06E-4 2.0 1.15E-1 1.0 1.23E-1 0.9
    128 1.99E-4 2.0 3.65E-2 1.0 2.98E-2 1.0 1.27E-4 2.0 5.78E-2 1.0 6.29E-2 1.0
    ν=1E-2
    8 3.80E-1 2.87 1.99E-2 2.81E-2 8.76E-1 7.43E-3
    16 9.79E-2 2.0 1.47 1.0 1.01E-2 1.0 7.69E-3 1.9 4.54E-1 0.9 4.36E-3 0.8
    32 2.47E-2 2.0 7.44E-1 1.0 5.16E-3 1.0 2.00E-3 1.9 2.30E-1 1.0 2.36E-3 0.9
    64 6.20E-3 2.0 3.73E-1 1.0 2.63E-3 1.0 5.06E-4 2.0 1.15E-1 1.0 1.23E-3 0.9
    128 1.55E-3 2.0 1.87E-1 1.0 1.33E-3 1.0 1.27E-4 2.0 5.78E-2 1.0 6.28E-4 1.0
    ν=1E-4
    8 3.51E+1 2.91E+2 2.41E-2 2.81E-2 8.76E-1 1.43E-4
    16 9.06 2.0 1.51E+2 0.9 1.24E-2 1.0 7.69E-3 1.9 4.54E-1 0.9 2.40E-5 2.6
    32 2.29 2.0 7.65E+1 1.0 6.36E-3 1.0 2.00E-3 1.9 2.30E-1 1.0 1.29E-5 0.9
    64 5.74E-1 2.0 3.84E+1 1.0 3.24E-3 1.0 5.06E-4 2.0 1.15E-1 1.0 9.36E-6 0.5
    128 1.44E-1 2.0 1.92E+1 1.0 1.64E-3 1.0 1.27E-4 2.0 5.78E-2 1.0 5.53E-6 0.8
    ν=1E-6
    8 3.50E+3 2.92E+4 2.41E-2 2.81E-2 8.76E-1 2.08E-4
    16 9.05E+2 2.0 1.51E+4 0.9 1.24E-2 1.0 7.69E-3 1.9 4.54E-1 0.9 5.40E-5 1.9
    32 2.29E+2 2.0 7.65E+3 1.0 6.37E-3 1.0 2.00E-3 1.9 2.30E-1 1.0 1.37E-5 2.0
    64 5.74E+1 2.0 3.84E+3 1.0 3.25E-3 1.0 5.06E-4 2.0 1.15E-1 1.0 3.40E-6 2.0
    128 1.44E+1 2.0 1.92E+3 1.0 1.64E-3 1.0 1.27E-4 2.0 5.78E-2 1.0 8.26E-7 2.0
    ν=1E-8
    8 3.50E+5 2.92E+6 2.41E-2 2.81E-2 8.76E-1 2.09E-4
    16 9.05E+4 2.0 1.51E+6 0.9 1.24E-2 1.0 7.69E-3 1.9 4.54E-1 0.9 5.44E-5 1.9
    32 2.29E+4 2.0 7.65E+5 1.0 6.37E-3 1.0 2.00E-3 1.9 2.30E-1 1.0 1.39E-5 2.0
    64 5.74E+3 2.0 3.84E+5 1.0 3.25E-3 1.0 5.06E-4 2.0 1.15E-1 1.0 3.50E-6 2.0
    128 1.44E+3 2.0 1.92E+5 1.0 1.64E-3 1.0 1.27E-4 2.0 5.78E-2 1.0 8.80E-7 2.0

     | Show Table
    DownLoad: CSV

    In this test, let Ω=(0,1)×(0,1) and the exact solution u and p be,

    u=(3πsin(πx)3sin(πy)2cos(πy)3πsin(πx)2sin(πy)3cos(πx)) and p=sin(πx).

    In this test, the top (y=1), left (x=0), and bottom (y=0) boundaries are assumed to be the Dirichlet boundary conditions. The right boundary (x=1) employs the Neumann boundary condition.

    We perform convergence tests on a sequence of meshes with varying viscosity values ν. The error profiles for the velocity and pressure solutions are plotted in Figures 35, in which we vary the mesh size h. The numerical results are similar to the two examples above. Figure 3 demonstrates the velocity errors measured in the L2-norm. When we use the non-pressure-robust scheme Algorithm 2.2/2.4, the errors converge at the second order for different values of ν. However, the velocity error depends on ν and the pressure. As the pressure error does not dominate the velocity errors, the error increases as a factor 1ν. This means that Algorithm 2.2/2.4 fails to produce reliable numerical solutions for the velocity. In contrast, Algorithm 2.3/2.4 outperforms Algorithm 2.2/2.4 and produces a robust numerical simulation. The velocity errors show an invariant behavior for varying viscosity values ν.

    Figure 3.  Example 4.3: Convergence results for Algorithm 2.2/2.4 (Left) and Algorithm 2.3/2.4 (Right) for velocity errors measured in L2-norm.
    Figure 4.  Example 4.3: Convergence results for Algorithm 2.2/2.4 (Left) and Algorithm 2.3/2.4 (Right) for velocity errors measured in H1-norm.
    Figure 5.  Example 4.3: Convergence results for Algorithm 2.2/2.4 (Left) and Algorithm 2.3/2.4 (Right) for pressure errors measured in L2-norm.

    The H1-error of the velocity is plotted in Figure 4. We observe the same behavior and can draw the same conclusion as above.

    Lastly, the L2-error of the pressure is plotted in Figure 5. Algorithm 2.2/2.4 first produces a better pressure approximation before the dominance of pressure error hp1. However, as the viscosity variable ν decreases, the pressure term will dominate the error, i.e., hp1hνu2. Thus, pressure errors show a constant behavior on the same mesh with decreasing values of ν. On the other hand, Algorithm 2.3/2.4 outperforms Algorithm 2.2/2.4. For small ν, Algorithm 2.3/2.4 produces much better pressure solutions. All of the above tests validate our theoretical conclusions.

    In this paper, we enhanced the divergence-free WG finite element method proposed in [3] by modifying the load function via the velocity reconstruction operator. Our proposed algorithm results in a symmetric positive definite matrix, and the velocity error is pressure-robust. Moreover, we illustrated the procedure for recovering the pressure variables. Numerical experiments are presented to validate the theoretical results. As a future work plan, we will consider the development of an effective preconditioner, which may improve the efficiency further. In addition, the construction of a high-order (k>2) divergence-free basis will be given and analyzed in our future work.

    The authors declare they have not used Artificial Intelligence (AI) tools in the creation of this article.

    Jay Chu is partially supported by the Ministry of Science and Technology of Taiwan under the research grant MOST 111-2115-M-007-012. Xiaozhe Hu is partially supported by the National Science Foundation under the grant DMS-2208267. Lin Mu is partially supported by the National Science Foundation under the grant DMS-2309557.

    The authors declare there are no conflicts of interest.



    [1] Owa FD (2013) Water pollution:Sources, effects, control and management. Mediterranean J. Social Sci 4:66. https://doi.org/10.5901/mjss.2013.v4n8p65 doi: 10.5901/mjss.2013.v4n8p65
    [2] Kumar RDH, Lee SM (2012) Water pollution and treatment technologies. J Environ Anal Toxicol 2: e103. https://doi.org/10.4172/2161-0525.1000e103
    [3] Ministry of Environmental Protection, MEP releases the 2014 Report on the state of environment in China, 2014. Available from: https://english.mee.gov.cn/News_service/news_release/201506/t20150612_303436.shtml.
    [4] Miao Y, Fan C, Guo J (2012) China's water environmental problems and improvement measures. Environ Resour Econ 3:43-44.
    [5] World Bank Group, Pakistan - Strategic country environmental assessment, Main Report no. 36946-PK World Bank, 2006. Available from: http://documents.worldbank.org/curated/en/132221468087836074/Main-report
    [6] Cutler DM, Miller G (2005) The role of public health improvements in health advances:The twentieth-century United States. Demography 42:1-22. https://doi.org/10.1353/dem.2005.0002 doi: 10.1353/dem.2005.0002
    [7] Jalan J, Ravallion M (2003) Does piped water reduce diarrhea for children in rural India? J Econ 112:153-173. https://doi.org/10.1016/S0304-4076(02)00158-6 doi: 10.1016/S0304-4076(02)00158-6
    [8] Roushdy R, Sieverding M, Radwan H (2012) The impact of water supply and sanitation on child health: Evidence from Egypt. New York Population Council, New York, 1–72. Available from: https://doi.org/10.31899/pgy3.1016
    [9] Lu YL, Song S, Wang RS, et al. (2015) Impacts of soil and water pollution on food safety and health risks in China. Environ Int 77:5-15. https://doi.org/10.1016/j.envint.2014.12.010 doi: 10.1016/j.envint.2014.12.010
    [10] Lin NF, Tang J, Ismael HSM (2000) Study on environmental etiology of high incidence areas of liver cancer in China. World J. Gastroenterol 6:572-576.
    [11] Morales-Suarez-Varela MM, Llopis-Gonzalez A, Tejerizo-Perez ML (1995) Impact of nitrates in drinking water on cancer mortality in Valencia, Spain. Eur J Epidemiol 11:15-21. https://doi.org/10.1007/BF01719941 doi: 10.1007/BF01719941
    [12] Ebenstein A (2012) The consequences of industrialization:Evidence from water pollution and digestive cancers in China. Rev Econ Stats 94:186-201. https://doi.org/10.1162/REST_a_00150 doi: 10.1162/REST_a_00150
    [13] Teh CY, Budiman PM, Shak KPY, et al. (2016) Recent advancement of coagulation-flocculation and its application in wastewater treatment. Ind Eng Chem Res 55:4363-4389. https://doi.org/10.1021/acs.iecr.5b04703 doi: 10.1021/acs.iecr.5b04703
    [14] Pontius FW (1990) Water quality and treatment. (4th Ed), New York: McGrawHill, Inc.
    [15] Xiaofan Z, Shaohong Y, Lili M, et al. (2015) The application of immobilized microorganism technology in wastewater treatment. 2nd International Conference on Machinery, Materials Engineering, Chemical Engineering and Biotechnology (MMECEB 2015). Pp. 103–106.
    [16] Malovanyy M, Masikevych A, Masikevych Y, et al. (2022) Use of microbiocenosis immobilized on carrier in technologies of biological treatment of surface and wastewater. J Ecol Eng 23:34-43.https: //doi.org/10.12911/22998993/151146
    [17] Wang L, Cheng WC, Xue ZF, et al. (2023) Study on Cu-and Pb-contaminated loess remediation using electrokinetic technology coupled with biological permeable reactive barrier. J Environ Manage 348:119348. https://doi.org/10.1016/j.jenvman.2023.119348 doi: 10.1016/j.jenvman.2023.119348
    [18] Wang L, Cheng WC, Xue ZF, et al. (2024) Struvite and ethylenediaminedisuccinic acid (EDDS) enhance electrokinetic-biological permeable reactive barrier removal of copper and lead from contaminated loess. J Environ Manage 360:121100. https://doi.org/10.1016/j.jenvman.2024.121100 doi: 10.1016/j.jenvman.2024.121100
    [19] Dombrovskiy KO, Rylskyy OF, Gvozdyak PI (2020) The periphyton structural organization on the fibrous carrier "viya" over the waste waters purification from the oil products. Hydrobiol J 56:87-96. https://doi.org/10.1615/HydrobJ.v56.i3.70 doi: 10.1615/HydrobJ.v56.i3.70
    [20] Zhang Y, Piao M, He L, (2020) Immobilization of laccase on magnetically separable biochar for highly efficient removal of bisphenol A in water. RSC Adv 10:4795-4804. https://doi.org/10.1039/C9RA08800H doi: 10.1039/C9RA08800H
    [21] Najim AA, Radeef AY, al-Doori I, et al. (2024) Immobilization:the promising technique to protect and increase the efficiency of microorganisms to remove contaminants. J Chem Technol Biotechnol 99:1707-1733. https://doi.org/10.1002/jctb.7638 doi: 10.1002/jctb.7638
    [22] Zhang K, Luo X, Yang L, et al. (2021) Progress toward hydrogels in removing heavy metals from water:Problems and solutions-A review. ACS ES&T Water 1:1098-1116. https://doi.org/10.1021/acsestwater.1c00001 doi: 10.1021/acsestwater.1c00001
    [23] Olaniran NS (1995) Environment and health: An introduction, In Olaniran NS et al. (Eds) Environment and Health. Lagos, Nigeria: Macmillan, for NCF, 34–151.
    [24] Singh G, Kumari B, Sinam G, (2018) Fluoride distribution and contamination in the water, soil and plants continuum and its remedial technologies, an Indian perspective-A review.Environ Poll239:95-108. https://doi.org/10.1016/j.envpol.2018.04.002 doi: 10.1016/j.envpol.2018.04.002
    [25] Schwarzenbach RP, Escher BI, Fenner K, (2006) The challenge of micropollutants in aquatic systems. Science 313:1072-1077. https://doi.org/10.1126/science.1127291 doi: 10.1126/science.1127291
    [26] Ma J, Ding Z, Wei G, et al. (2009) Sources of water pollution and evolution of water quality in the Wuwei basin of Shiyang river, Northwest China.J Environ Manage90:1168-1177. https://doi.org/10.1016/j.jenvman.2008.05.007 doi: 10.1016/j.jenvman.2008.05.007
    [27] Abdulmumini A, Gumel SM, Jamil G (2015) Industrial effluents as major source of water pollution in Nigeria:An overview. Am J Chem Appl 1:45-50.
    [28] Fakayode O (2005) Impact assessment of industrial effluent on water quality of the receiving Alaro River in Ibadan. Nigerian Afr J Environ Assoc Manage 10:1-13
    [29] Begum A, Ramaiah M, Harikrishna, I, (2009) Heavy metals pollution and chemical profile of Cauvery of river water. J Chem 6:45-52. https://doi.org/10.1155/2009/154610 doi: 10.1155/2009/154610
    [30] Sunita S, Darshan M, Jayita T, (2014) A comparative analysis of the physico-chemical properties and heavy metal pollution in three major rivers across India. Int J Sci Res 3:1936-1941.
    [31] Li J, Yang Y, Huan H, et al. (2016) Method for screening prevention and control measures and technologies based on groundwater pollution intensity assessment. Sci Total Environ 551:143-154. https://doi.org/10.1016/j.scitotenv.2015.12.152 doi: 10.1016/j.scitotenv.2015.12.152
    [32] Yuanan H, Hefa C (2013) Water pollution during China's industrial transition. Environ Dev 8:57-73. https://doi.org/10.1016/j.envdev.2013.06.001 doi: 10.1016/j.envdev.2013.06.001
    [33] Li W, Hongbin W (2021) Control of urban river water pollution is studied based on SMS. Environ Technol Innov 22:101468. https://doi.org/10.1016/j.eti.2021.101468 doi: 10.1016/j.eti.2021.101468
    [34] Swapnil MK (2014) Water pollution and public health issues in Kolhapur city in Maharashtra. Int J Sci Res Pub 4:1-6.
    [35] Qijia C, Yong H, Hui W, (2019) Diversity and abundance of bacterial pathogens in urban rivers impacted by domestic sewage. Environ Pollut 249:24-35. https://doi.org/10.1016/j.envpol.2019.02.094 doi: 10.1016/j.envpol.2019.02.094
    [36] Ministry of Environmental Protection (MEP), 2011 China State of the Environment. China Environmental Science Press, Beijing, China, 2012.
    [37] Gao C, Zhang T (2010) Eutrophication in a Chinese context:Understanding various physical and socio-economic aspects. Ambio 39:385-393. https://doi.org/10.1007/s13280-010-0040-5 doi: 10.1007/s13280-010-0040-5
    [38] Chanti BP, Prasadu DK (2015) Impact of pharmaceutical wastes on human life and environment. RCJ 8:67-70.
    [39] World Health Organization, WHO (2013) Water sanitation and health. Available from: https://www.who.int/teams/environment-climate-change-and-health/water-sanitation-and-health
    [40] Sayadi MH, Trivedy RK, Pathak RK (2010) Pollution of pharmaceuticals in environment. J Ind Pollut Control
    [41] Fick J, Söderström H, Lindberg RH, (2009) Contamination of surface, ground, and drinking water from pharmaceutical production. Environ Toxicol Chem 28:2522-2527. https://doi.org/10.1897/09-073.1 doi: 10.1897/09-073.1
    [42] Rosen M, Welander T, Lofqvist A (1998) Development of a new process for treatment of a pharmaceutical wastewater. Water Sci Technol 37:251-258.
    [43] Niraj ST, Attar SJ, Mosleh MM (2011) Sewage/wastewater treatment technologies. Sci Revs Chem Commun 1:18-24
    [44] Martins SCS, Martins CM, Oliveira, Fiúza LMCG, (2013) Immobilization of microbial cells:A promising tool for treatment of toxic pollutants in industrial wastewater. Afr J Biotechnol 12:4412-4418. https://doi.org/10.5897/AJB12.2677 doi: 10.5897/AJB12.2677
    [45] Cassidy MB, Lee H, Trevors JT (1996) Environmental applications of immobilized microbial cells:A review. J Ind Microbiol 16:79-101. https://doi.org/10.1007/BF01570068 doi: 10.1007/BF01570068
    [46] Wada M, Kato J, Chibata I (1979) A new immobilization of microbial cells. Appl Microbiol Biotechnol 8:241-247. https://doi.org/10.1007/BF00508788 doi: 10.1007/BF00508788
    [47] Park JK, Chang HN (2000) Microencapsulation of microbial cells. Biotechnol Adv 18:303-319. https://doi.org/10.1016/S0734-9750(00)00040-9 doi: 10.1016/S0734-9750(00)00040-9
    [48] Mrudula S, Shyam N (2012) Immobilization of Bacillus megaterium MTCC 2444 by Ca-alginate entrapment method for enhanced alkaline protease production. Braz Arch Biol Technol 55:135-144. https://doi.org/10.1590/S1516-89132012000100017 doi: 10.1590/S1516-89132012000100017
    [49] Xia B, Zhao Q, Qu Y (2010) The research of different immobilized microorganisms' technologies and carriers in sewage disposal. Sci Technol Inf 1:698-699.
    [50] Han M, Zhang C, Ho SH (2023) Immobilized microalgal system:An achievable idea for upgrading current microalgal wastewater treatment. ESE 1:100227. https://doi.org/10.1016/j.ese.2022.100227 doi: 10.1016/j.ese.2022.100227
    [51] Wang Z, Ishii S, Novak PJ (2021) Encapsulating microorganisms to enhance biological nitrogen removal in wastewater:recent advancements and future opportunities. Environ Sci:Water Sci Technol 7:1402-1416. https://doi.org/10.1039/D1EW00255D doi: 10.1039/D1EW00255D
    [52] Tong CY, Derek CJ (2023) Bio-coatings as immobilized microalgae cultivation enhancement:A review. Sci Total Environ 20:163857. https://doi.org/10.1016/j.scitotenv.2023.163857 doi: 10.1016/j.scitotenv.2023.163857
    [53] Dzionek A, Wojcieszyńska D, Guzik U (2022) Use of xanthan gum for whole cell immobilization and its impact in bioremediation-a review. Bioresour Technol 351:126918. https://doi.org/10.1016/j.biortech.2022.126918 doi: 10.1016/j.biortech.2022.126918
    [54] Blanco A, Sampedro MA, Sanz B, et al. (2023) Immobilization of non-viable cyanobacteria and their use for heavy metal adsorption from water. In Environmental biotechnology and cleaner bioprocesses, CRC Press, 2023,135–153. https://doi.org/10.1201/9781003417163-14
    [55] Saini S, Tewari S, Dwivedi J, et al. (2023) Biofilm-mediated wastewater treatment:a comprehensive review. Mater Adv 4:1415-1443. https://doi.org/10.1039/D2MA00945E doi: 10.1039/D2MA00945E
    [56] Bouabidi ZB, El-Naas MH, Zhang Z (2019) Immobilization of microbial cells for the biotreatment of wastewater:A review. Environ Chem Lett 17:241-257. https://doi.org/10.1007/s10311-018-0795-7 doi: 10.1007/s10311-018-0795-7
    [57] Fortman DJ, Brutman JP, De Hoe GX, et al. (2018) Approaches to sustainable and continually recyclable cross-linked polymers. ACS Sustain Chem Eng 6:11145-11159. https://doi.org/10.1021/acssuschemeng.8b02355 doi: 10.1021/acssuschemeng.8b02355
    [58] Mahmoudi C, Tahraoui DN, Mahmoudi H, et al. (2024) Hydrogels based on proteins cross-linked with carbonyl derivatives of polysaccharides, with biomedical applications. Int J Mol Sci 25:7839. https://doi.org/10.3390/ijms25147839 doi: 10.3390/ijms25147839
    [59] Waheed A, Mazumder MAJ, Al-Ahmed A, et al. (2019) Cell encapsulation. In Jafar MM, Sheardown H, Al-Ahmed A (Eds), Functional biopolymers, Polymers and polymeric composites: A reference series. Springer, Cham. https://doi.org/10.1007/978-3-319-95990-0_4
    [60] Chen S, Arnold WA, Novak PJ (2021) Encapsulation technology to improve biological resource recovery:recent advancements and research opportunities. Environ Sci Water Res Technol 7:16-23. https://doi.org/10.1039/D0EW00750A doi: 10.1039/D0EW00750A
    [61] Tripathi A, Melo JS (2021) Immobilization Strategies:Biomedical, Bioengineering and Environmental Applications (Gels Horizons:From Science to Smart Materials), 676. https://doi.org/10.1007/978-981-15-7998-1p
    [62] Górecka E, Jastrzębska M (2011) Immobilization techniques and biopolymer carriers. Biotechnol Food Sci 75:65-86.
    [63] Lopez A, Lazaro N, Marques AM (1997) The interphase technique:a simple method of cell immobilization in gel-beads. J of Microbiol Methods 30:231-234. https://doi.org/10.1016/S0167-7012(97)00071-7 doi: 10.1016/S0167-7012(97)00071-7
    [64] Ramakrishna SV, Prakasha RS (1999) Microbial fermentations with immobilized cells. Curr Sci 77:87-100.
    [65] Saberi RR, Skorik YA, Thakur VK, et al. (2021) Encapsulation of plant biocontrol bacteria with alginate as a main polymer material. Int J Mol Sci 22:11165. https://doi.org/10.3390/ijms222011165 doi: 10.3390/ijms222011165
    [66] Mohidem NA, Mohamad M, Rashid MU, et al. (2023) Recent advances in enzyme immobilisation strategies:An overview of techniques and composite carriers.J Compos Sci7:488. https://doi.org/10.3390/jcs7120488 doi: 10.3390/jcs7120488
    [67] Thomas D, O'Brien T, Pandit A (2018) Toward customized extracellular niche engineering:progress in cell-entrapment technologies. Adv Mater 30:1703948. https://doi.org/10.1002/adma.201703948 doi: 10.1002/adma.201703948
    [68] Farasat A, Sefti MV, Sadeghnejad S, et al. (2017) Mechanical entrapment analysis of enhanced preformed particle gels (PPGs) in mature reservoirs.J Pet Sci Eng157:441-450. https://doi.org/10.1016/j.petrol.2017.07.028 doi: 10.1016/j.petrol.2017.07.028
    [69] Sampaio CS, Angelotti JA, Fernandez-Lafuente R, et al. (2022) Lipase immobilization via cross-linked enzyme aggregates:Problems and prospects-A review. Int J Biol Macromol 215:434-449. https://doi.org/10.1016/j.ijbiomac.2022.06.139 doi: 10.1016/j.ijbiomac.2022.06.139
    [70] Picos-Corrales LA, Morales-Burgos AM, Ruelas-Leyva JP, et al. (2023) Chitosan as an outstanding polysaccharide improving health-commodities of humans and environmental protection.Polymers 15:526. https://doi.org/10.3390/polym15030526 doi: 10.3390/polym15030526
    [71] Gill J, Orsat V, Kermasha S (2018) Optimization of encapsulation of a microbial laccase enzymatic extract using selected matrices. Process Biochem 65:55-61. https://doi.org/10.1016/j.procbio.2017.11.011 doi: 10.1016/j.procbio.2017.11.011
    [72] Navarro JM, Durand G (1977) Modification of yeast metabolism by immobilization onto porous glass. Eur J Appl Microbiol 4:243-254. https://doi.org/10.1007/BF00931261 doi: 10.1007/BF00931261
    [73] Alkayyali T, Cameron T, Haltli B, et al. (2019) Microfluidic and cross-linking methods for encapsulation of living cells and bacteria-A review. Analytica Chimica Acta 1053:1-21. https://doi.org/10.1016/j.aca.2018.12.056 doi: 10.1016/j.aca.2018.12.056
    [74] Guisan JM, Fernandez-Lorente G, Rocha-Martin J, et al. (2022) Enzyme immobilization strategies for the design of robust and efficient biocatalysts. CRGSC 35:100593. https://doi.org/10.1016/j.cogsc.2022.100593 doi: 10.1016/j.cogsc.2022.100593
    [75] Qi F, Jia Y, Mu R, et al. (2021) Convergent community structure of algal bacterial consortia and its effect on advanced wastewater treatment and biomass production. Sci Rep 11:21118. https://doi.org/10.1038/s41598-021-00517-x doi: 10.1038/s41598-021-00517-x
    [76] Sharma M, Agarwal S, Agarwal MR, et al. (2023) Recent advances in microbial engineering approaches for wastewater treatment:a review. Bioengineered 14:2184518. https://doi.org/10.1080/21655979.2023.2184518 doi: 10.1080/21655979.2023.2184518
    [77] Cortez S, Nicolau A, Flickinger MC, et al. (2017) Biocoatings:A new challenge for environmental biotechnology. Biochem Eng J 121:25-37. https://doi.org/10.1016/j.bej.2017.01.004 doi: 10.1016/j.bej.2017.01.004
    [78] Zhao LL, Pan B, Zhang W, et al. (2011) Polymer-supported nanocomposites for environmental application:a review Chem. Eng. J., 170:381-394. https://doi.org/10.1016/j.cej.2011.02.071
    [79] Flickinger MC, Schottel JL, Bond DR (2007) Scriven Painting and printing living bacteria:Engineering nanoporous biocatalytic coatings to preserve microbial viability and intensify reactivity. Biotechnol Prog 23:2-17. https://doi.org/10.1021/bp060347r doi: 10.1021/bp060347r
    [80] Martynenko NN, Gracheva IM, Sarishvili NG, et al. (2004) Immobilization of champagne yeasts by inclusion into cryogels of polyvinyl alcohol:Means of preventing cell release from the carrier matrix. Appl Biochem Microbiol 40:158-164. https://doi.org/10.1023/B:ABIM.0000018919.13036.19 doi: 10.1023/B:ABIM.0000018919.13036.19
    [81] Yang D, Shu-Qian F, Yu S, et al. (2015) A novel biocarrier fabricated using 3D printing technique for wastewater treatment. Sci Rep 5:12400. https://doi.org/10.1038/srep12400 doi: 10.1038/srep12400
    [82] Ayilara MS, Babalola OO (2023) Bioremediation of environmental wastes:the role of microorganisms. Front Agron 5:1183691. https://doi.org/10.3389/fagro.2023.1183691 doi: 10.3389/fagro.2023.1183691
    [83] Kumar V, Garg VK, Kumar S, et al. (2022) Omics for environmental engineering and microbiology systems (Florida:CRC Press). https://doi.org/10.1201/9781003247883
    [84] Liu L, Wu Q, Miao X, et al. (2022) Study on toxicity effects of environmental pollutants based on metabolomics:A review. Chemosphere 286:131815. https://doi.org/10.1016/j.chemosphere.2021.131815 doi: 10.1016/j.chemosphere.2021.131815
    [85] Zdarta J, Jankowska K, Bachosz K, et al. (2021) Enhanced wastewater treatment by immobilized enzymes. Current Pollution Reports 7:167-179. https://doi.org/10.1007/s40726-021-00183-7 doi: 10.1007/s40726-021-00183-7
    [86] Freeman A, Lilly MD (1998) Effect of processing parameters on the feasibility and operational stability of immobilized viable microbial cells. Enzyme Microb Technol 23 335-345. https://doi.org/10.1016/S0141-0229(98)00046-5
    [87] Ligler FS, Taitt CR (2011) Optical biosensors: Today and tomorrow, Elsevier, Pp. 151.
    [88] Ismail E, José D, Gonçalves V, et al. (2015) Principles, techniques, and applications of biocatalyst immobilization for industrial application. Appl Microbiol Biotechnol 99:2065-2082. https://doi.org/10.1007/s00253-015-6390-y doi: 10.1007/s00253-015-6390-y
    [89] Anselmo AM, Novais JM (1992) Degradation of phenol by immobilized mycelium of Fusarium flocciferum in continuous culture. Water Sci Technol 25:161-168.https://doi.org/10.2166/wst.1992.0024 doi: 10.2166/wst.1992.0024
    [90] Liu Z, Yang H, Jia S (1992) Study on decolorization of dyeing wastewater by mixed bacterial cells immobilized in polyvinyl alcohol (PVA). China Environ Sci 13:2-6.
    [91] Guomin C, Zhao Q, Gong J (2001) Study on nitrogen removal from wastewater in a new co-immobilized cells membrane bioreactor. Acta Sci Circumst. 21:189-193.
    [92] Jogdand VG, Chavan PA, Ghogare PD, et al. (2012) Remediation of textile industry waste-water using immobilized Aspergillus terreus. Eur J Exp Biol 2:1550-1555
    [93] Adlercreutz P, Holst O, Mattiasson B (1982) Oxygen supply to immobilized cells:2. Studies on a coimmobilized algae-bacteria preparation with in situ oxygen generation. Enzyme Microb Tech 4:395-400. https://doi.org/10.1016/0141-0229(82)90069-2 doi: 10.1016/0141-0229(82)90069-2
    [94] Chevalier P, de la Noüe J (1988) Behavior of algae and bacteria co-immobilized in carrageenan, in a fluidized bed. Enzyme Microb Tech 10:19-23. https://doi.org/10.1016/0141-0229(88)90093-2 doi: 10.1016/0141-0229(88)90093-2
    [95] Wikström P, Szwajcer E, Brodelius P, et al. (1982) Formation of α-keto acids from amino acids using immobilized bacteria and algae. Biotechnol Lett 4:153-158. https://doi.org/10.1007/BF00144316 doi: 10.1007/BF00144316
    [96] Serebrennikova MK, Golovina EE, Kuyukina MS, et al. (2017) A consortium of immobilized Rhodococci for oil field wastewater treatment in a column bioreactor. Appl Biochem Microbiol 53:435-440. https://doi.org/10.1134/S0003683817040123 doi: 10.1134/S0003683817040123
    [97] Choi M, Chaudhary R, Lee M, et al. (2020) Enhanced selective enrichment of partial nitritation and anammox bacteria in a novel two-stage continuous flow system using flat-type poly(vinylalcohol) cryogel films. Bioresour Technol 300:122546. https://doi.org/10.1016/j.biortech.2019.122546 doi: 10.1016/j.biortech.2019.122546
    [98] Yordanova G, Ivanova D, Godjevargova T, et al. (2009) Biodegradation of phenol by immobilized Aspergillus awamori NRRL 3112 on modified polyacrylonitrile membrane. Biodegradation 20:717-726. https://doi.org/10.1007/s10532-009-9259-x doi: 10.1007/s10532-009-9259-x
    [99] Tekere M (2019) Microbial bioremediation and different bioreactors designs applied. In Biotechnology and Bioengineering; IntechOpen: London, UK, Pp. 1–19. https://doi.org/10.5772/intechopen.83661
    [100] Liu SH, Zeng ZT, Niu QY, et al. (2019) Influence of immobilization on phenanthrene degradation by Bacillus sp. P1 in the presence of Cd (II). Sci Total Environ 655:1279-1287. https://doi.org/10.1016/j.scitotenv.2018.11.272 doi: 10.1016/j.scitotenv.2018.11.272
    [101] Liu SH, Lin HH, Lai CY, et al. (2019) Microbial community in a pilot-scale biotrickling filter with cell-immobilized biochar beads and its performance in treating toluene-contaminated waste gases. Int Biodeterior Biodegrad 144:104743. https://doi.org/10.1016/j.ibiod.2019.104743 doi: 10.1016/j.ibiod.2019.104743
    [102] Önnby L, Pakade V, Mattiasson B, et al. (2012) Polymer composite adsorbents using particles of molecularly imprinted polymers or aluminium oxide nanoparticles for treatment of arsenic contaminated waters. Water Res 46:4111-4120. https://doi.org/10.1016/j.watres.2012.05.028 doi: 10.1016/j.watres.2012.05.028
    [103] Baimenov A, Berillo D, Azat S, et al. (2020) Removal of Cd2+ from water by use of super-macroporous cryogels and comparison to commercial adsorbents. Polymers 12:2405. https://doi.org/10.3390/polym12102405 doi: 10.3390/polym12102405
    [104] Baimenov AZ, Berillo DA, Moustakas K, et al. (2020) Efficient removal of mercury (II) from water by use of cryogels and comparison to commercial adsorbents under environmentally relevant conditions. J Hazard Mater 399:123056. https://doi.org/10.1016/j.jhazmat.2020.123056 doi: 10.1016/j.jhazmat.2020.123056
    [105] Safonova E, Kvitko KV, Iankevitch MI, et al. (2004) Biotreatment of industrial wastewater by selected algal-bacterial consortia. Eng Life Sci 4:347-353. https://doi.org/10.1002/elsc.200420039 doi: 10.1002/elsc.200420039
    [106] Blanco A, Sanz B, Llama MJ, et al. (1999) Biosorption of heavy metals to immobilized Phormidium laminosum biomass. J Biotechnol 69:227-240. https://doi.org/10.1016/S0168-1656(99)00046-2 doi: 10.1016/S0168-1656(99)00046-2
    [107] Somerville HJ, Mason JR, Ruffell RN (1977) Benzene degradation by bacterial cells immobilized in polyacrylamide gel. Appl Microbiol Biotechnol 4:75-85. https://doi.org/10.1007/BF00929158 doi: 10.1007/BF00929158
    [108] Tsai SL, Lin CW, Wu CH, et al. (2013) Kinetics of xenobiotic biodegradation by the Pseudomonas sp. YATO411 strain in suspension and cell-immobilized beads. J Taiwan Inst Chem Eng 44:303-309. https://doi.org/10.1016/j.jtice.2012.11.004 doi: 10.1016/j.jtice.2012.11.004
    [109] Akhtar N, Saeed A, Iqbal M (2003) Chlorella sorokiniana immobilized on the biomatrix of vegetable sponge of Luffa cylindrica:a new system to remove cadmium from contaminated aqueous medium. Bioresour Technol 88:163-165. https://doi.org/10.1016/S0960-8524(02)00289-4 doi: 10.1016/S0960-8524(02)00289-4
    [110] Saeed A, Iqbal M (2006) Immobilization of blue green microalgae on loofa sponge to biosorb cadmium in repeated shake flask batch and continuous flow fixed bed column reactor system. World J Microb Biotechnol 22:775-782. https://doi.org/10.1007/s11274-005-9103-3 doi: 10.1007/s11274-005-9103-3
    [111] Rangasayatorn N, Pokethitiyook P, Upatahm ES, et al. (2004) Cadmium biosorption by cells of Spirulina platensis TISTR 8217 immobilized in alginate and silica gel. Environ Int 30:57-63. https://doi.org/10.1016/S0160-4120(03)00146-6 doi: 10.1016/S0160-4120(03)00146-6
    [112] Mohamed AA, Ahmed MA, Mahmoud ME, et al. (2019) Bioremediation of a pesticide and selected heavy metals in wastewater from various sources using a consortium of microalgae and cyanobacteria. Slov Vet Res 56:61-74.
    [113] Aslıyüce S, Denizli A (2017) Design of PHEMA Cryogel as Bioreactor Matrices for Biological Cyanide Degradation from Waste-water. Hacet J Biol Chem 45:639-645. https://doi.org/10.15671/HJBC.2018.208 doi: 10.15671/HJBC.2018.208
    [114] Suner SS, Sahiner N (2018) Humic acid particle embedded super porous gum Arabic cryogel network for versatile use. Polym Adv Technol 29:151-159. https://doi.org/10.1002/pat.4097 doi: 10.1002/pat.4097
    [115] Sharma A, Bhat S, Vishnoi T, et al. (2013). Three-dimensional super macroporous carrageenan-gelatin cryogel matrix for tissue engineering applications. BioMed Res Int 2013: 478279.
    [116] Le Noir M, Plieva FM, Mattiasson B (2009) Removal of endocrine-disrupting compounds from water using macroporous molecularly imprinted cryogels in a moving-bed reactor. J Sep Sci 32:1471-1479. https://doi.org/10.1002/jssc.200800670 doi: 10.1002/jssc.200800670
    [117] See S, Lim PE, Lim JW, et al. (2005) Evaluation of o-cresol removal using PVA-cryogel-immobilised biomass enhanced by PAC. Water SA 41:55-60. https://doi.org/10.4314/wsa.v41i1.8 doi: 10.4314/wsa.v41i1.8
    [118] Gao S, Wang Y, Diao X, et al. (2010) Effect of pore diameter and cross-linking method on the immobilization efficiency of Candida rugose lipase in SBA-15. Bioresour Technol 101:3830-3837. https://doi.org/10.1016/j.biortech.2010.01.023 doi: 10.1016/j.biortech.2010.01.023
    [119] Stepanov N, Efremenko E (2018) Deceived concentrated immobilized cells as biocatalyst for intensive bacterial cellulose production from various sources. Catalysts 8:33. https://doi.org/10.3390/catal8010033 doi: 10.3390/catal8010033
    [120] Zaushitsyna O, Berillo D, Kirsebom H, et al. (2014) Cryostructured and crosslinked viable cells forming monoliths suitable for bioreactor applications. Top Catal 57:339-348. https://doi.org/10.1007/s11244-013-0189-9 doi: 10.1007/s11244-013-0189-9
    [121] Qian L, Zhang H (2011) Controlled freezing and freeze drying:A versatile route for porous and micro-/nano-structured materials. J Chem Technol Biotechnol 86:172-184. https://doi.org/10.1002/jctb.2495 doi: 10.1002/jctb.2495
  • Reader Comments
  • © 2024 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Metrics

Article views(1951) PDF downloads(205) Cited by(1)

Figures and Tables

Figures(2)  /  Tables(3)

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return

Catalog