Research article Topical Sections

Composite scaffolds of chitosan/polycaprolactone functionalized with protein of Mytilus californiensis for bone tissue regeneration

  • Received: 25 October 2021 Revised: 05 February 2022 Accepted: 13 March 2022 Published: 30 March 2022
  • Nowadays, the treatment for bone damage remains a significant challenge. As a result, the development of bioactive three-dimensional scaffolds for bone regeneration has become a key area of study within tissue engineering. This research is focused on the evaluation of the properties of Chitosan (Ch)/Polycaprolactone (PCL) scaffolds with the Mytilus californiensis protein by Thermally Induced Phase Separation (TIPS). This study used the extrapalleal fluid protein from Mytilus californiensis because it increases biological processes that support bone regeneration. Two methodologies were used for the scaffolds functionalization: (I) an immersion process in a solution with the protein and (II) the protein direct addition during the scaffold synthesis. The scaffolds were analyzed by Fourier Transformed Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), and Mechanical Compression test to determine the composition, morphology, and mechanical properties of each material. In vitro analysis of biodegradation, bioactivity, and biocompatibility were also performed. The scaffolds with the protein added directly presented superior properties in the tests of bioactivity and cellular proliferation, making these composites attractive for the area of bone regeneration.

    Citation: Miguel-Angel Rojas-Yañez, Claudia-Alejandra Rodríguez-González, Santos-Adriana Martel-Estrada, Laura-Elizabeth Valencia-Gómez, Claudia-Lucia Vargas-Requena, Juan-Francisco Hernández-Paz, María-Concepción Chavarría-Gaytán, Imelda Olivas-Armendáriz. Composite scaffolds of chitosan/polycaprolactone functionalized with protein of Mytilus californiensis for bone tissue regeneration[J]. AIMS Materials Science, 2022, 9(3): 344-358. doi: 10.3934/matersci.2022021

    Related Papers:

  • Nowadays, the treatment for bone damage remains a significant challenge. As a result, the development of bioactive three-dimensional scaffolds for bone regeneration has become a key area of study within tissue engineering. This research is focused on the evaluation of the properties of Chitosan (Ch)/Polycaprolactone (PCL) scaffolds with the Mytilus californiensis protein by Thermally Induced Phase Separation (TIPS). This study used the extrapalleal fluid protein from Mytilus californiensis because it increases biological processes that support bone regeneration. Two methodologies were used for the scaffolds functionalization: (I) an immersion process in a solution with the protein and (II) the protein direct addition during the scaffold synthesis. The scaffolds were analyzed by Fourier Transformed Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), and Mechanical Compression test to determine the composition, morphology, and mechanical properties of each material. In vitro analysis of biodegradation, bioactivity, and biocompatibility were also performed. The scaffolds with the protein added directly presented superior properties in the tests of bioactivity and cellular proliferation, making these composites attractive for the area of bone regeneration.



    加载中


    [1] Su X, Wang T, Guo S (2021) Applications of 3D printed bone tissue engineering scaffolds in the stem cell field. Regen Ther 16: 63-72. https://doi.org/10.1016/j.reth.2021.01.007 doi: 10.1016/j.reth.2021.01.007
    [2] Safari B, Aghanejad A, Roshangar L, et al. (2020) Osteogenic effects of the bioactive small molecules and minerals in the scaffold-based bone tissue engineering. Colloid Surface B 198: 111462. https://doi.org/10.1016/j.colsurfb.2020.111462 doi: 10.1016/j.colsurfb.2020.111462
    [3] Xia P, Zhang K, Yan S, et al. (2021) Biomimetic, biodegradable, and osteoinductive Microgels with open porous structure and excellent injectability for construction of microtissues for bone tissue engineering. Chem Eng J 414: 128714. https://doi.org/10.1016/j.cej.2021.128714 doi: 10.1016/j.cej.2021.128714
    [4] Prasad A (2021) State of art review on bioabsorbable polymeric scaffolds for bone tissue engineering. Mater Today Proc 44: 1391-1400. https://doi.org/10.1016/j.matpr.2020.11.622 doi: 10.1016/j.matpr.2020.11.622
    [5] Ma C, Wang H, Chi Y, et al. (2021) Preparation of oriented collagen fiber scaffolds and its application in bone tissue engineering. Appl Mater Today 22: 100902. https://doi.org/10.1016/j.apmt.2020.100902 doi: 10.1016/j.apmt.2020.100902
    [6] Park J, Lee SJ, Jung TG, et al. (2021) Surface modification of a three-dimensional polycaprolactone scaffold by polydopamine, biomineralization, and BMP-2 immobilization for potential bone tissue applications. Colloids Surf B 199: 111528. https://doi.org/10.1016/j.colsurfb.2020.111528 doi: 10.1016/j.colsurfb.2020.111528
    [7] Kundu K, Afshar A, Katti DR, et al. (2020) Composite nanoclay-hydroxyapatite-polymer fiber scaffolds for bone tissue engineering manufactured using pressurized gyration. Compos Sci Technol 202: 108598. https://doi.org/10.1016/j.compscitech.2020.108598 doi: 10.1016/j.compscitech.2020.108598
    [8] Shaabani A, Sedghi R, Motasadizadeh H, et al. (2021) Self-healable conductive polyurethane with the body temperature‐responsive shape memory for bone tissue engineering. Chem Eng J 411: 128449. https://doi.org/10.1016/j.cej.2021.128449 doi: 10.1016/j.cej.2021.128449
    [9] Wang L, Lu R, Hou J, et al. (2020) Application of injectable silk fibroin/graphene oxide hydrogel combined with bone marrow mesenchymal stem cells in bone tissue engineering. Colloids Surf A 604: 125318. https://doi.org/10.1016/j.colsurfa.2020.125318 doi: 10.1016/j.colsurfa.2020.125318
    [10] Wuriantika MI, Utomo J, Nurhuda M, et al. (2021) Nanostructure, porosity and tensile strength of PVA/Hydroxyapatite composite nanofiber for bone tissue engineering. Mater Today Proc 44: 3203-3206. https://doi.org/10.1016/j.matpr.2020.11.438 doi: 10.1016/j.matpr.2020.11.438
    [11] Pandit A, Indurkar A, Deshpande C, et al. (2021) A systematic review of physical techniques for chitosan degradation. Carbohydr Polym 2: 100033. https://doi.org/10.1016/j.carpta.2021.100033 doi: 10.1016/j.carpta.2021.100033
    [12] Madni A, Kousar R, Naeem N, et al. (2021) Recent advancements in applications of chitosan-based biomaterials for skin tissue engineering. J Bioresour Bioprod 6: 128714. https://doi.org/10.1016/j.jobab.2021.01.002 doi: 10.1016/j.jobab.2021.01.002
    [13] Li J, Zhuang S (2020) Antibacterial activity of chitosan and its derivatives and their interaction mechanism with bacteria: Current state and perspectives. Eur Polym J 138: 109984. https://doi.org/10.1016/j.eurpolymj.2020.109984 doi: 10.1016/j.eurpolymj.2020.109984
    [14] Bulbul YE, Uzunoglu T, Dilsiz N, et al. (2020) Investigation of nanomechanical and morphological properties of silane-modified halloysite clay nanotubes reinforced polycaprolactone bio-composite nanofibers by atomic force microscopy. Polym Test 92: 106877. https://doi.org/10.1016/j.polymertesting.2020.106877 doi: 10.1016/j.polymertesting.2020.106877
    [15] Voniatis C, Barczikai D, Gyulai G, et al. (2021) Fabrication and characterisation of electrospun Polycaprolactone/Polysuccinimide composite meshes. J Mol Liq 323: 115094. https://doi.org/10.1016/j.molliq.2020.115094 doi: 10.1016/j.molliq.2020.115094
    [16] Zhao YQ, Yang JH, Ding X, et al. (2020) Polycaprolactone/polysaccharide functional composites for low-temperature fused deposition modelling. Bioact Mater 5: 185-191. https://doi.org/10.1016/j.bioactmat.2020.02.006 doi: 10.1016/j.bioactmat.2020.02.006
    [17] Jaramillo-Martínez S, Vargas-Requena C, Rodríguez-Gónzalez C, et al. (2019) Effect of extrapallial protein of Mytilus californianus on the process of in vitro biomineralization of chitosan scaffolds. Heliyon 5: e02252. https://doi.org/10.1016/j.heliyon.2019.e02252
    [18] Urbanek O, Sajkiewicz P, Pierini F (2017) The effect of polarity in the electrospinning process on PCL/chitosan nanofibres' structure, properties and efficiency of surface modification. Polymer 124: 168-175. https://doi.org/10.1016/j.polymer.2017.07.064 doi: 10.1016/j.polymer.2017.07.064
    [19] Zhang C, Zhai T, Turng LS, et al. (2015) Morphological, mechanical, and crystallization behavior of polylactide/polycaprolactone blends compatibilized by L-lactide/caprolactone copolymer. Ind Eng Chem Res 54: 9505-9511. https://doi.org/10.1021/acs.iecr.5b02134 doi: 10.1021/acs.iecr.5b02134
    [20] Sarasam A, Madihally SV (2005) Characterization of chitosan—polycaprolactone blends for tissue engineering applications. Biomaterials 26: 5500-5508. https://doi.org/10.1016/j.biomaterials.2005.01.071 doi: 10.1016/j.biomaterials.2005.01.071
    [21] Fabian H, Mä ntele W (2006) Infrared spectroscopy of proteins, Handbook of Vibrational Spectroscopy, 1 Ed., New York: Wiley Online Library.
    [22] Fadaie M, Mirzaei E, Geramizadeh B, et al. (2018) Incorporation of nanofibrillated chitosan into electrospun PCL nanofibers makes scaffolds with enhanced mechanical and biological properties. Carbohydr Polym 199: 628-640. https://doi.org/10.1016/j.carbpol.2018.07.061 doi: 10.1016/j.carbpol.2018.07.061
    [23] Martel-Estrada SA, Olivas-Armendáriz I, Santos-Rodríguez E, et al. (2014) Evaluation of in vitro bioactivity of Chitosan/Mimosa tenuiflora composites. Mater Lett 119: 146-149. https://doi.org/10.1016/j.matlet.2014.01.004 doi: 10.1016/j.matlet.2014.01.004
    [24] Wang F, Guo Y, Lv R, et al. (2020) Development of nano-tricalcium phosphate/polycaprolactone/platelet-rich plasma biocomposite for bone defect regeneration. Arab J Chem 13: 7160-7169. https://doi.org/10.1016/j.arabjc.2020.07.021 doi: 10.1016/j.arabjc.2020.07.021
    [25] Bil M, Hipś I, Mrówka P, et al. (2020) Studies on enzymatic degradation of multifunctional composite consisting of chitosan microspheres and shape memory polyurethane matrix. Polym Degrad Stab 182: 109392. https://doi.org/10.1016/j.polymdegradstab.2020.109392 doi: 10.1016/j.polymdegradstab.2020.109392
    [26] Houreh AB, Masaeli E, Nasr-Esfahani MH (2021) Chitosan/polycaprolactone multilayer hydrogel: A sustained Kartogenindelivery model for cartilage regeneration. Int J Biol Macromol 177: 589-600. https://doi.org/10.1016/j.ijbiomac.2021.02.122 doi: 10.1016/j.ijbiomac.2021.02.122
    [27] Ureña J, Tsipas S, Jiménez-Morales A, et al. (2018) In-vitro study of the bioactivity and cytotoxicity response of Ti surfaces modified by Nb and Mo diffusion treatments. Surf Coat Technol 335: 148-158. https://doi.org/10.1016/j.surfcoat.2017.12.009 doi: 10.1016/j.surfcoat.2017.12.009
    [28] Choong CSN, Triffitt JT (2005) Improved bone cellular activity through the use of calcium phosphate coated polymeric scaffolds. Bone 36: S103-S479.
    [29] Bayrak GK, Demirtaş TT, Gümüşderelioğlu M (2012) Microwave-induced biomimetic approach for hydroxyapatite coatings of chitosan scaffolds. Carbohydr Polym 157: 803-813. https://doi.org/10.1016/j.carbpol.2016.10.016 doi: 10.1016/j.carbpol.2016.10.016
    [30] Chavira GNJ (2012) Evaluación de la capacidad de la proteína del fluido extrapalial de Mytilus Edulis de coordinar calcio[Dissertation]. Autonomous University of Ciudad Juárez (In Mexico).
    [31] Saravanan S, Leena RS, Selvamurugan N (2016) Chitosan based biocomposite scaffolds for bone tissue engineering. Int J Biol Macromol 93: 1354-1365. https://doi.org/10.1016/j.ijbiomac.2016.01.112 doi: 10.1016/j.ijbiomac.2016.01.112
    [32] Xu Z, Zhao F, Chen H, et al. (2019) Nutritional properties and osteogenic activity of enzymatic hydrolysates of proteins from the blue mussel (Mytilus edulis). Food Funct 10: 7745-7754. https://doi.org/10.1039/C9FO01656B doi: 10.1039/C9FO01656B
    [33] Xu Z, Fan F, Chen H, et al. (2021) Absorption and transport of a Mytilus edulis-derived peptide with the function of preventing osteoporosis. Food Funct 12: 2102-2111. https://doi.org/10.1039/D0FO02353A doi: 10.1039/D0FO02353A
    [34] He B, Zhao J, Ou Y, et al. (2018) Biofunctionalized peptide nanofiber-based composite scaffolds for bone regeneration. Mat Sci Eng C 90: 728-738. https://doi.org/10.1016/j.msec.2018.04.063 doi: 10.1016/j.msec.2018.04.063
    [35] Guasco Herrera C, Chávez Servín JL, Ferriz Martínez RA, et al. (2014) Poliaminas: pequeños gigantes de la regulación metabólica. REB 33: 51-57.
    [36] Roque J, Molera J, Vendrell-Saz M, et al. (2004) Crystal size distributions of induced calcium carbonate crystals in polyaspartic acid and Mytilus edulis acidic organic proteins aqueous solutions. J Cryst Growth 262: 543-553. https://doi.org/10.1016/j.jcrysgro.2003.10.052 doi: 10.1016/j.jcrysgro.2003.10.052
    [37] Hyung JH, Ahn CB, Je JY (2018) Blue mussel (Mytilus edulis) protein hydrolysate promotes mouse mesenchymal stem cell differentiation into osteoblasts through up-regulation of bone morphogenetic protein. Food Chem 242: 156-161. https://doi.org/10.1016/j.foodchem.2017.09.043 doi: 10.1016/j.foodchem.2017.09.043
  • Reader Comments
  • © 2022 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Metrics

Article views(2512) PDF downloads(221) Cited by(1)

Article outline

Figures and Tables

Figures(5)  /  Tables(2)

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return

Catalog