Changes in nucleosome formation at gene promoters in the archiascomycetous yeast Saitoella complicata
Department of Biotechnology, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan
Topical Section: Yeast Functional Genomics
We measured the degree of nucleosome formation at the gene promoters in trichostatin A-treated (1, 2, and 3 μg/mL) cells of the archiascomycete Saitoella complicata and those in enlarged S. complicata cells after zymolyase treatment. TSA-treated and enlarged cells showed similar changes in nucleosome occupancy in five out of six positions in the gene promoters. These results suggest that changes in nucleosome formation at the gene promoters could serve as stress response mechanisms elicited in response to spheroplast (zymolyase treatment) and TSA treatment. In addition, we demonstrated that changes in nucleosome position occurred mainly in cells treated with 1 μg/mL TSA, whereas cells treated with 2 and 3 μg/mL TSA did not exhibit significant changes in the degree of nucleosome formation.
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