Research article

Influence of cholesterol on human calcitonin channel formation. Possible role of sterol as molecular chaperone

  • Received: 31 October 2018 Accepted: 24 January 2019 Published: 01 February 2019
  • The interplay between lipids and embedded proteins in plasma membrane is complex. Membrane proteins affect the stretching or disorder of lipid chains, transbilayer movement and lateral organization of lipids, thus influencing biological processes such as fusion or fission. Membrane lipids can regulate some protein functions by modulating their structure and organization. Cholesterol is a lipid of cell membranes that has been intensively investigated and found to be associated with some membrane proteins and to play an important role in diseases. Human calcitonin (hCt), an amyloid-forming peptide, is a small peptide hormone. The oligomerization and fibrillation processes of hCt can be modulated by different factors such as pH, solvent, peptide concentration, and chaperones. In this work, we investigated the role of cholesterol in hCt incorporation and channel formation in planar lipid membranes made up of palmitoyl-oleoyl-phosphatidylcholine in which no channel activity had been found. The results obtained in this study indicate that cholesterol promotes hCt incorporation and channel formation in planar lipid membranes, suggesting a possible role of sterol as a lipid target for hCt.

    Citation: Daniela Meleleo, Cesare Sblano. Influence of cholesterol on human calcitonin channel formation. Possible role of sterol as molecular chaperone[J]. AIMS Biophysics, 2019, 6(1): 23-38. doi: 10.3934/biophy.2019.1.23

    Related Papers:

  • The interplay between lipids and embedded proteins in plasma membrane is complex. Membrane proteins affect the stretching or disorder of lipid chains, transbilayer movement and lateral organization of lipids, thus influencing biological processes such as fusion or fission. Membrane lipids can regulate some protein functions by modulating their structure and organization. Cholesterol is a lipid of cell membranes that has been intensively investigated and found to be associated with some membrane proteins and to play an important role in diseases. Human calcitonin (hCt), an amyloid-forming peptide, is a small peptide hormone. The oligomerization and fibrillation processes of hCt can be modulated by different factors such as pH, solvent, peptide concentration, and chaperones. In this work, we investigated the role of cholesterol in hCt incorporation and channel formation in planar lipid membranes made up of palmitoyl-oleoyl-phosphatidylcholine in which no channel activity had been found. The results obtained in this study indicate that cholesterol promotes hCt incorporation and channel formation in planar lipid membranes, suggesting a possible role of sterol as a lipid target for hCt.


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    Acknowledgments



    The authors acknowledge Anthony Green for proofreading and providing linguistic advice.

    Conflict of interest



    All authors declare no conflicts of interest in this paper

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