Seed treatments with essential oils protect radish seedlings against drought

Joshua D. Klein; Amanda Firmansyah; Nurhaya Panga; Waffa Abu-Aklin; Miriam Dekalo-Keren; Tanya Gefen; Ronit Kohen; Yonit Raz Shalev; Nativ Dudai; Lea Mazor; Joshua D. Klein; Amanda Firmansyah; Nurhaya Panga; Waffa Abu-Aklin; Miriam Dekalo-Keren; Tanya Gefen; Ronit Kohen; Yonit Raz Shalev; Nativ Dudai; Lea Mazor

doi:10.3934/agrfood.2017.4.345

AIMS Agriculture and Food

2017, Volume 2, Issue 4: 345-353. doi: 10.3934/agrfood.2017.4.345

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Seed treatments with essential oils protect radish seedlings against drought

1.
Institute of Plant Sciences, ARO-Volcani Center, Rishon LeZion, Israel
2.
Hassanudin University, Makassar, Indonesia
3.
Official Israeli Seed Testing Laboratory, ARO-Volcani Center, Rishon LeZion, Israel
4.
Newe Ya'ar Research Center, Ramat Yishay, Israel

Received: 23 July 2017 Accepted: 10 October 2017 Published: 19 October 2017

Establishment of seedlings of economic crops is often reduced if there is not a steady supply of water. Essential oils (EO) from plants are increasingly used instead of synthetic chemicals to protect plant and animal products against biotic and abiotic stresses. We investigated priming radish seeds by soaking or by matriconditioning with synthetic or natural compounds as a means of inducing resistance to drought stress, thus maintaining crop yield. Priming radish seeds for two hours in solutions of essential oils (EO) thymol and carvacrol derived from Origanum syriacum, with “oregano natural product” (ONP; a solution of the residue remaining after EO extraction), or with the gibberellin synthesis inhibitor trinexapac ethyl (TE), was much more effective in inducing drought resistance than was matriconditioning with the same compounds in sawdust for two days. The latter treatment induced considerable fungal and bacterial infection in treated seeds if the substrate-matrix was not heat-treated beforehand. The increase in specific leaf area in plants from treated seeds was mostly consistent with an increase in leaf water content. Seed treatments with EO, ONP, and especially TE led to a three-fold increase in radish seedling survival compared with water-treated controls, when 21 day-old seedlings were irrigated after 6 days of drought. Under drought conditions, seedlings from treated seeds had a 2–3-fold increase in relative water content increased 2–3-fold, while membrane permeability decreased 20–50-fold as a result of the treatments. However, the physical benefits of the treatments often did not correlate with treatment-induced increases in physiological parameters such as pigments (chlorophyll, carotenoid, anthocyanin), pigment ratios (chlorophyll a/b, carotenoid/chlorophyll), or antioxidant activity. Seed treatments with biostimulants can be as effective as treatments with synthetic compounds in inducing drought resistance in seedlings.
- Trinexapac-ethyl,
- carvacrol,
- thymol,
- Oregano syriacum,
- carotenoids,
- anthocyanin,
- DPPH,
- chlorophyll,
- relative water content,
- ion leakage,
- seed priming
Citation: Joshua D. Klein, Amanda Firmansyah, Nurhaya Panga, Waffa Abu-Aklin, Miriam Dekalo-Keren, Tanya Gefen, Ronit Kohen, Yonit Raz Shalev, Nativ Dudai, Lea Mazor. Seed treatments with essential oils protect radish seedlings against drought[J]. AIMS Agriculture and Food, 2017, 2(4): 345-353. doi: 10.3934/agrfood.2017.4.345

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Abstract

Establishment of seedlings of economic crops is often reduced if there is not a steady supply of water. Essential oils (EO) from plants are increasingly used instead of synthetic chemicals to protect plant and animal products against biotic and abiotic stresses. We investigated priming radish seeds by soaking or by matriconditioning with synthetic or natural compounds as a means of inducing resistance to drought stress, thus maintaining crop yield. Priming radish seeds for two hours in solutions of essential oils (EO) thymol and carvacrol derived from Origanum syriacum, with “oregano natural product” (ONP; a solution of the residue remaining after EO extraction), or with the gibberellin synthesis inhibitor trinexapac ethyl (TE), was much more effective in inducing drought resistance than was matriconditioning with the same compounds in sawdust for two days. The latter treatment induced considerable fungal and bacterial infection in treated seeds if the substrate-matrix was not heat-treated beforehand. The increase in specific leaf area in plants from treated seeds was mostly consistent with an increase in leaf water content. Seed treatments with EO, ONP, and especially TE led to a three-fold increase in radish seedling survival compared with water-treated controls, when 21 day-old seedlings were irrigated after 6 days of drought. Under drought conditions, seedlings from treated seeds had a 2–3-fold increase in relative water content increased 2–3-fold, while membrane permeability decreased 20–50-fold as a result of the treatments. However, the physical benefits of the treatments often did not correlate with treatment-induced increases in physiological parameters such as pigments (chlorophyll, carotenoid, anthocyanin), pigment ratios (chlorophyll a/b, carotenoid/chlorophyll), or antioxidant activity. Seed treatments with biostimulants can be as effective as treatments with synthetic compounds in inducing drought resistance in seedlings.

References

[1]	Knight H, Knight MR (2001) Abiotic stress signaling pathways: specificity and cross-talk. Trends Plant Sci 6: 262-267.
[2]	Paparella S, Araújo SS, Rossi G, et al. (2015) Seed priming: state of the art and new perspectives. Plant Cell Rep 34: 1281-1293. doi: 10.1007/s00299-015-1784-y
[3]	Taylor AG, Allen PS, Bennett MA, et al. (1998) Seed enhancements. Seed Sci Res 8: 245-256.
[4]	Taylor AG, Klein DE, Whitlow TH (1988) SMP: Solid matrix priming of seeds. Sci Hortic 37: 1-11. doi: 10.1016/0304-4238(88)90146-X
[5]	Ilyas S (2006) Seed treatments using matriconditioning to improve vegetable seed quality. J Agronomi Indonesia (Indonesian J Agron) 34: 124-132.
[6]	De Azeredo GA, Stamford TL, Nunes PC, et al. (2011) Combined application of essential oils from Origanum vulgare L. and Rosmarinus officinalis L. to inhibit bacteria and autochthonous microflora associated with minimally processed vegetables. Food Res Int 44: 1541-1548.
[7]	Ye X, Ling T, Xue Y, et al. (2016) Thymol mitigates cadmium stress by regulating glutathione levels and reactive oxygen species homeostasis in tobacco seedlings. Molecules 21: 1339-1353. doi: 10.3390/molecules21101339
[8]	Martino LD, Mancini E, Almeida LFRD, et al. (2010) The antigerminative activity of twenty-seven monoterpenes. Molecules 15: 6630-6637. doi: 10.3390/molecules15096630
[9]	Arteca RN (2013) Plant growth substances: principles and applications. Springer Science & Business Media.
[10]	Havkin-Frenkel D, Bakto Natural Preservatives Llc (2017) Recovery of residual plant components after distillation of essential oils. U.S. Patent 9,572,849 [P].
[11]	Rademacher W (2016) Chemical regulators of gibberellin status and their application in plant production. Ann Plant Rev 49: 359-404.
[12]	Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzimol 148: 350-382. doi: 10.1016/0076-6879(87)48036-1
[13]	Stafford HA (1966) Regulatory mechanisms in anthocyanin biosynthesis in first internodes of Sorghum vulgare: effect of presumed inhibitors of protein synthesis. Plant Physiol 41: 953-961. doi: 10.1104/pp.41.6.953
[14]	Molyneux P (2004) The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 26: 211-219.
[15]	Rorden C (2007) Available from: http://www.cabiatl.com/mricro/ezanova/
[16]	Aharoni N (1989) Interrelationship between ethylene and growth regulators in the senescence of lettuce leaf discs. J Plant Growth Regul 8: 309-317.
[17]	Klein JD, Shalev YR, Cohen S, et al. (2016) Postharvest handling of "etrog" citron (Citrus medica, L.) fruit. Isr J Plant Sci 63: 64-75. doi: 10.1080/07929978.2016.1159409
[18]	Horvathova E, Navarova J, Galova E, et al. (2014) Assessment of antioxidative, chelating, and DNA-protective effects of selected essential oil components (eugenol, carvacrol, thymol, borneol, eucalyptol) of plants and intact Rosmarinus officinalis oil. J Agric Food Chem 62: 6632-6639. doi: 10.1021/jf501006y
[19]	Heckman NL, Horst GL, Gaussoin RE (2001) Influence of trinexapac-ethyl on specific leaf weight and chlorophyll content of Poa pratensis. Int Turfgrass Soc Res J 9: 287-290.
[20]	Phavaphutanon L, Hebbe Y, Cohen S, et al. (2004) Trinexapac-ethyl and paclobutrazol protect against drought in hot pepper plants. HortScience 39: 897.
[21]	Martín JA, Solla A, García-Vallejo MC, et al. (2012) Chemical changes in Ulmus minor xylem tissue after salicylic acid or carvacrol treatments are associated with enhanced resistance to Ophiostoma novo-ulmi. Phytochem 83: 104-109.
[22]	Martín JA, Solla A, Domingues MR, et al. (2008) Exogenous phenol increase resistance of Ulmus minor to Dutch elm disease through formation of suberin-like compounds on xylem tissues. Environ Exp Bot 64:97-104. doi: 10.1016/j.envexpbot.2008.05.004
[23]	Gitelson AA, Gamon JA, Solovchenko A (2017) Multiple drivers of seasonal change in PRI: Implications for photosynthesis 1. Leaf level. Remote Sens Environ 191: 110-116. doi: 10.1016/j.rse.2016.12.014
[24]	Todaka D, Zhao Y, Yoshida T (2017) Temporal and spatial changes in gene expression, metabolite accumulation and phytohormone content in rice seedlings grown under drought stress conditions. Plant J 90: 61-78. doi: 10.1111/tpj.13468
[25]	Ashraf M, Ahmad A, McNeilly T (2001) Growth and photosynthetic characteristics in pearl millet under water stress and different potassium supply. Photosynthetica 39: 389-394. doi: 10.1023/A:1015182310754
[26]	Silva PA, Oliveira IV, Rodrigues KC (2016) Leaf gas exchange and multiple enzymatic and non-enzymatic antioxidant strategies related to drought tolerance in two oil palm hybrids. Trees 30: 203-214. doi: 10.1007/s00468-015-1289-x
[27]	Kähkönen MP, Heinonen M (2003) Antioxidant activity of anthocyanins and their aglycons. J Agric Food Chem 51: 28-633.

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