Effects of inoculation sources on the enrichment and performance of anode bacterial consortia in sensor typed microbial fuel cells

Phuong Tran; Linh Nguyen; Huong Nguyen; Bong Nguyen; Linh Nong; Linh Mai; Huyen Tran; Thuy Nguyen; Hai Pham; Phuong Tran; Linh Nguyen; Huong Nguyen; Bong Nguyen; Linh Nong; Linh Mai; Huyen Tran; Thuy Nguyen; Hai Pham

doi:10.3934/bioeng.2016.1.60

AIMS Bioengineering

2016, Volume 3, Issue 1: 60-74. doi: 10.3934/bioeng.2016.1.60

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Effects of inoculation sources on the enrichment and performance of anode bacterial consortia in sensor typed microbial fuel cells

1.
Research group for Physiology and Applications of Microorganisms (PHAM group) at Center for Life Science Research, Faculty of Biology, Vietnam National University – University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Vietnam
2.
Department of Microbiology, Faculty of Biology, Vietnam National University – University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Vietnam

Received: 20 September 2015 Accepted: 05 January 2016 Published: 07 January 2016

Microbial fuel cells are a recently emerging technology that promises a number of applications in energy recovery, environmental treatment and monitoring. In this study, we investigated the effect of inoculating sources on the enrichment of electrochemically active bacterial consortia in sensor-typed microbial fuel cells (MFCs). Several MFCs were constructed, operated with modified artificial wastewater and inoculated with different microbial sources from natural soil, natural mud, activated sludge, wastewater and a mixture of those sources. After enrichment, the MFCs inoculated with the natural soil source generated higher and more stable currents (0.53±0.03 mA), in comparisons with the MFCs inoculated with the other sources. The results from denaturing gradient gel electrophoresis (DGGE) showed that there were significant changes in bacterial composition from the original inocula to the enriched consortia. Even more interestingly, Pseudomonas sp. was found dominant in the natural soil source and also in the corresponding enriched consortium. The interactions between Pseudomonas sp. and other species in such a community are probably the key for the effective and stable performance of the MFCs.
- microbial fuel cell,
- bioelectrochemical system,
- sensor typed microbial fuel cell,
- anode bacterial consortia,
- Pseudomonas sp
Citation: Phuong Tran, Linh Nguyen, Huong Nguyen, Bong Nguyen, Linh Nong , Linh Mai, Huyen Tran, Thuy Nguyen, Hai Pham. Effects of inoculation sources on the enrichment and performance of anode bacterial consortia in sensor typed microbial fuel cells[J]. AIMS Bioengineering, 2016, 3(1): 60-74. doi: 10.3934/bioeng.2016.1.60

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Abstract

Microbial fuel cells are a recently emerging technology that promises a number of applications in energy recovery, environmental treatment and monitoring. In this study, we investigated the effect of inoculating sources on the enrichment of electrochemically active bacterial consortia in sensor-typed microbial fuel cells (MFCs). Several MFCs were constructed, operated with modified artificial wastewater and inoculated with different microbial sources from natural soil, natural mud, activated sludge, wastewater and a mixture of those sources. After enrichment, the MFCs inoculated with the natural soil source generated higher and more stable currents (0.53±0.03 mA), in comparisons with the MFCs inoculated with the other sources. The results from denaturing gradient gel electrophoresis (DGGE) showed that there were significant changes in bacterial composition from the original inocula to the enriched consortia. Even more interestingly, Pseudomonas sp. was found dominant in the natural soil source and also in the corresponding enriched consortium. The interactions between Pseudomonas sp. and other species in such a community are probably the key for the effective and stable performance of the MFCs.

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