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Isolation and characterization of the Staphylococcus aureus bacteriophage vB_SauS_SA2

Jia Wang Feiyang Zhao Huzhi Sun Qian Wang Can Zhang Wenhua Liu Ling Zou Qiang Pan Huiying Ren

*Corresponding author: Huiying Ren renren0228@sina.com

microbiology2019,3,285doi:10.3934/microbiol.2019.3.285

A novel bacteriophage vB_SauS_SA2 (hereafter designated SA2) that infects Staphylococcus aureus was isolated. At a multiplicity of infection (MOI) of 0.1, phage SA2 had a latent period of about 10 min with a burst size of 293 PFUs/infected cell (PFU, plaque forming unit). Phage SA2 had a double-stranded DNA genome with a length of 89,055 bp and a G + C content of 31.9%. The genome contained 130 open reading frames (ORFs), 28 of which had assigned functions, and 18 were unique. One tRNA gene (tRNAAsn) was discovered, and no virulence genes were identified. Its genome showed very low similarity with phage genomes deposited in public databases (75% nucleotide identity and 7% query coverage). The unique characteristics of phage SA2 led to the proposal of a new Siphoviridae genus named ‘SA2likevirus’.

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