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Effectiveness of synthetic calcite doped with Fe-EDDHSA as a slow-release Fe source: In-vitro experiment on kiwifruit (Actinidia chinensis var. deliciosa) plants

Baldi Elena Marino Grazia Muzzi Enrico Marzadori Claudio Ciavatta Claudio Tavoni Marta Di Giosia Matteo Calvaresi Matteo Falini Giuseppe Zerbetto Francesco Toselli Moreno

*Corresponding author: Baldi Elena elena.baldi7@unibo.it


Doped calcite (Fe-EDDHSA/CaCO3) was experimentally produced. The hypothesis of the present experiment is that, when roots get in contact with Fe-EDDHSA/CaCO3, the extrusion of H+ decreases the pH and dissolves calcite with subsequent release of Fe that becomes available for roots. The aim of the experiment was to determine whether doped calcite might represent a slow-release Fe source for in-vitro grown kiwifruit plantlets.
The root elongation media used in the experiment had pH 8.0 and differed from each other for Fe supply as follow: Control medium that contained complete Murashige and Skoog salt mixture, including FeSO4 and Na2EDTA; calcite medium enriched with Fe-EDDHSA/CaCO3 as the only Fe source; −Fe medium without Fe.
The absence of FeSO4 in the medium caused a reduction of plantlet growth. The final pH was higher with calcite medium than in control and −Fe. The addition of Fe-EDDHSA/CaCO3 increased Fe shoot concentration when compared with the −Fe medium. The data of the present experiment show the potential Fe slow release ability of Fe-EDDHSA/CaCO3; however, further investigation on Fe containing fertilizers should be conducted on potted plants to validate our results.

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