<?xml version="1.0" encoding="UTF-8" standalone="yes"?>
<doi_batch version="4.3.0">
	<!-->1.头标区<-->
    <head>
        <doi_batch_id>1</doi_batch_id>
        <timestamp>20180817022419</timestamp>
        <depositor>
            <name>Susan</name>
            <email_address>journal@aimsciences.org</email_address>
        </depositor>
        <registrant>American Institute of Mathematical Sciences</registrant>
    </head>
    <!-->2.主体<-->
    <body>
    <!-->AIMS Genetics<-->
        <journal>
        <!-->2.1 刊元数据<-->
            <journal_metadata language="en">
                <full_title></full_title>
                <abbrev_title></abbrev_title>
                <issn media_type="print"></issn>
                <doi_data>
                    <doi></doi>
                    <resource>http://www.aimsciences.org/journal/</resource>
                </doi_data>
            </journal_metadata>
            <!-->2.2 期元数据<-->
            <journal_issue>
                <publication_date media_type="print">
                    <month></month>
                    <year>2018</year>
                </publication_date>
                <journal_volume>
                    <volume>5</volume>
                </journal_volume>
                <issue>1</issue>
            </journal_issue>
            <!-->2.3 文章元数据<-->
                        <journal_article>
	            	            <titles>
                    <title><![CDATA[Our love-hate relationship with DNA barcodes, the Y2K problem, and the search for next generation barcodes]]></title>
                </titles>
	                            <contributors>
                                </contributors>
                <publication_date media_type="print">
                    <month></month>
                    <year>2018</year>
                </publication_date>
                
                <pages>
                    <first_page>1</first_page>
                    <last_page>23</last_page>
                </pages>
                <doi_data>
                    <doi>10.3934/genet.2018.1.1</doi>
                    <timestamp>2017-11-22 13:00:00.0</timestamp>
					<resource>http://www.aimsciences.org/article/id/1797</resource>
                </doi_data>
	                
                <citation_list>
               	                    <citation key="">
                        <journal_title><em>Proc R Soc Lond B</em></journal_title>
                        <volume>270</volume>
                        <author>     Hebert PDN, Cywinska A, Ball SL, et al.</author>
                        <first_page>313</first_page>
                        <last_page>321</last_page>
                        <cYear>2003</cYear>
                        <doi>10.1098/rspb.2002.2218</doi>
                        <article_title>Biological identifications through DNA barcodes</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Mar Bio Biotechnol</em></journal_title>
                        <volume>3</volume>
                        <author>     Folmer O, Black MB, Hoch W, et al.</author>
                        <first_page>294</first_page>
                        <last_page>299</last_page>
                        <cYear>1994</cYear>
                        <doi></doi>
                        <article_title>DNA primers for amplification of mitochondrial <em>cytochrome c oxidase</em> subunit I from diverse metazoan invertebrates</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>AIMS Genetics</em></journal_title>
                        <volume>4</volume>
                        <author>     Chen CS, Huang CT, Hseu RS</author>
                        <first_page>192</first_page>
                        <last_page>201</last_page>
                        <cYear>2017</cYear>
                        <doi>10.3934/genet.2017.3.192</doi>
                        <article_title>Evidence for two types of nrDNA existing in Chinese medicinal fungus <em>Ophiocordyceps sinensis</em></article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Adv Biol</em></journal_title>
                        <volume>2014</volume>
                        <author>Lebonah DE, Dileep A, Chandrasekhar K, et al.</author>
                        <first_page>541787</first_page>
                        <last_page></last_page>
                        <cYear>2014</cYear>
                        <doi></doi>
                        <article_title>DNA barcoding on bacteria: A review</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Ticks and Tick-borne Diseases</em> 8.</journal_title>
                        <volume></volume>
                        <author>Sperling JL, Silva-Brandao KL, Brandao MM, et al.</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi></doi>
                        <article_title>Comparison of bacterial 16S rRNA variable regions for microbiome surveys of ticks</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Methods Mol Biol</em></journal_title>
                        <volume>1245</volume>
                        <author>     de Vere N, Rich TC, Trinder SA, et al.</author>
                        <first_page>101</first_page>
                        <last_page>118</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1007/978-1-4939-1966-6_8</doi>
                        <article_title>DNA barcoding for plants</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Genomics</em></journal_title>
                        <volume>107</volume>
                        <author>     Heather JM, Chain B</author>
                        <first_page>1</first_page>
                        <last_page>8</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1016/j.ygeno.2015.11.003</doi>
                        <article_title>The sequence of sequencers: The history of sequencing DNA</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>whose problem. <em>Time Soc</em></journal_title>
                        <volume>7</volume>
                        <author>     Schwaller C</author>
                        <first_page>105</first_page>
                        <last_page>118</last_page>
                        <cYear>1998</cYear>
                        <doi></doi>
                        <article_title>'The millennium time bomb' or year 2000 problem: what problem</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Proc Nat Acad Sci USA</em></journal_title>
                        <volume>103</volume>
                        <author>     Hajibabaei M, Janzen DH, Burns JM, et al.</author>
                        <first_page>968</first_page>
                        <last_page>971</last_page>
                        <cYear>2006</cYear>
                        <doi>10.1073/pnas.0510466103</doi>
                        <article_title>DNA barcodes distinguish species of tropical Lepidoptera</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Technol Soc</em></journal_title>
                        <volume>22</volume>
                        <author>   Manion M, Evan WM</author>
                        <first_page>361</first_page>
                        <last_page>387</last_page>
                        <cYear>2000</cYear>
                        <doi>10.1016/S0160-791X(00)00015-4</doi>
                        <article_title>The Y2K problem and professional responsibility: a retrospective analysis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>In: Kulski JK, editor. <em>Next Generation Sequencing-Advances, Applications and Challenges</em>: InTech. pp. Available from: https://www.intechopen.com/books/next-generation-sequencing-advances-applications-and-challenges/next-generation-sequencing-an-overview-of-the-history-tools-and-omic-applications.</journal_title>
                        <volume></volume>
                        <author>Kulski JK</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2016</cYear>
                        <doi></doi>
                        <article_title>Next-Generation Sequencing-An Overview of the History, Tools, and "Omic" Applications</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>barcodinglife.org). <em>Mol Ecol Notes</em></journal_title>
                        <volume>7</volume>
                        <author>  Ratnasingham S, Hebert PDN</author>
                        <first_page>355</first_page>
                        <last_page>364</last_page>
                        <cYear>2007</cYear>
                        <doi>10.1111/j.1471-8286.2007.01678.x</doi>
                        <article_title>BOLD: The Barcode of Life Data System (http://www</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Ecol Resour</em></journal_title>
                        <volume>12</volume>
                        <author>   Taylor HR, Harris WE</author>
                        <first_page>377</first_page>
                        <last_page>388</last_page>
                        <cYear>2012</cYear>
                        <doi>10.1111/j.1755-0998.2012.03119.x</doi>
                        <article_title>An emergent science on the brink of irrelevance: a review of the past 8 years of DNA barcoding</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS Biol</em></journal_title>
                        <volume>2</volume>
                        <author>Moritz C, Cicero C</author>
                        <first_page>e354</first_page>
                        <last_page></last_page>
                        <cYear>2004</cYear>
                        <doi>10.1371/journal.pbio.0020354</doi>
                        <article_title>DNA Barcoding: Promise and Pitfalls</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Ecol</em></journal_title>
                        <volume>4</volume>
                        <author>   Duvernell DD, Aspinwall N</author>
                        <first_page>173</first_page>
                        <last_page>181</last_page>
                        <cYear>1995</cYear>
                        <doi>10.1111/j.1365-294X.1995.tb00206.x</doi>
                        <article_title>Introgression of <em>Luxilus cornutus</em> Mtdna into allopatric populations of <em>Luxilus chrysocephalus</em> (Teleostei, Cyprinidae) in Missouri and Arkansas</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Ecol</em></journal_title>
                        <volume>17</volume>
                        <author>   Good JM, Hird S, Reid N, et al.</author>
                        <first_page>1313</first_page>
                        <last_page>1327</last_page>
                        <cYear>2008</cYear>
                        <doi>10.1111/j.1365-294X.2007.03640.x</doi>
                        <article_title>Ancient hybridization and mitochondrial capture between two species of chipmunks</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS ONE</em></journal_title>
                        <volume>11</volume>
                        <author>Chambers EA, Hebert PDN</author>
                        <first_page>e0154363</first_page>
                        <last_page></last_page>
                        <cYear>2016</cYear>
                        <doi>10.1371/journal.pone.0154363</doi>
                        <article_title>Assessing DNA barcodes for species identification in North American reptiles and amphibians in natural history collections</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title></journal_title>
                        <volume></volume>
                        <author></author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear></cYear>
                        <doi></doi>
                        <article_title>McCullagh BS, Marcus JM (Submitted) When barcodes go bad: Exploring the limits of DNA barcoding with complete <em>Junonia</em> butterfly mitochondrial genomes <em>Submitted to Molecular Phylogenetics and</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Science</em></journal_title>
                        <volume>284</volume>
                        <author>   Bennett RF</author>
                        <first_page>438</first_page>
                        <last_page>439</last_page>
                        <cYear>1999</cYear>
                        <doi>10.1126/science.284.5413.438</doi>
                        <article_title>Technology-The Y2K problem</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Asia-Pacific Ent</em></journal_title>
                        <volume>18</volume>
                        <author>   McCullagh BS, Marcus JM</author>
                        <first_page>749</first_page>
                        <last_page>755</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1016/j.aspen.2015.08.006</doi>
                        <article_title>The complete mitochondrional genome of Lemon Pansy, <em>Junonia lemonias</em> (Lepidoptera: Nymphalidae: Nymphalinae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Proc Nat Acad Sci USA</em></journal_title>
                        <volume>101</volume>
                        <author>   Hebert PDN, Penton EH, Burns JM, et al.</author>
                        <first_page>14812</first_page>
                        <last_page>14817</last_page>
                        <cYear>2004</cYear>
                        <doi>10.1073/pnas.0406166101</doi>
                        <article_title>Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly <em>Astraptes fulgerator</em></article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>sas.upenn.edu.</journal_title>
                        <volume></volume>
                        <author>Janzen DH, Hallwachs W</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2005</cYear>
                        <doi></doi>
                        <article_title>Dynamic database for an inventory of the macrocaterpillar fauna, and its food plants and parasitoids, of Area de Conservacion Guanacaste (ACG), northwestern Costa Rica http://janzen</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Lepid Soc</em></journal_title>
                        <volume>61</volume>
                        <author>   Burns JM, Janzen DH, Hajibabaei M, et al.</author>
                        <first_page>138</first_page>
                        <last_page>153</last_page>
                        <cYear>2007</cYear>
                        <doi></doi>
                        <article_title>DNA barcodes of closely related (but morphologically and ecologically distinct) species of skipper butterflies (Hesperiidae) can differ by only one to three nucleotides</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>BMC Evol Biol</em></journal_title>
                        <volume>8</volume>
                        <author>Tavares ES, Baker AJ</author>
                        <first_page>81</first_page>
                        <last_page></last_page>
                        <cYear>2008</cYear>
                        <doi>10.1186/1471-2148-8-81</doi>
                        <article_title>Single mitochondrial gene barcodes reliably identify sister-species in diverse clades of birds</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Biol Lett</em></journal_title>
                        <volume>6</volume>
                        <author>   Hebert PDN, deWaard JR, Landry JF</author>
                        <first_page>359</first_page>
                        <last_page>362</last_page>
                        <cYear>2010</cYear>
                        <doi>10.1098/rsbl.2009.0848</doi>
                        <article_title>DNA barcodes for 1/1000 of the animal kingdom</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Metabarcoding and Metagenomics </em></journal_title>
                        <volume>1</volume>
                        <author>Vamos EE, Elbrecht V, Leese F</author>
                        <first_page>e14625</first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi>10.3897/mbmg.1.14625</doi>
                        <article_title>Short <em>COI</em> markers for freshwater macroinvertebrate metabarcoding</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>Available from: http://wwwcoreoceanorg/Dev2Goweb?id=255158.</journal_title>
                        <volume></volume>
                        <author>Stoeckle M, Bucklin A, Knowlton N, et al.</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2006</cYear>
                        <doi></doi>
                        <article_title>Census of Marine Life DNA Barcoding Protocol</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>BMC Genomics</em></journal_title>
                        <volume>9</volume>
                        <author>Meusnier I, Singer GAC, Landry JF, et al.</author>
                        <first_page>214</first_page>
                        <last_page></last_page>
                        <cYear>2008</cYear>
                        <doi>10.1186/1471-2164-9-214</doi>
                        <article_title>A universal DNA mini-barcode for biodiversity analysis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Syst Ent</em></journal_title>
                        <volume>40</volume>
                        <author>   Gemmell AP, Marcus JM</author>
                        <first_page>532</first_page>
                        <last_page>546</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1111/syen.12120</doi>
                        <article_title>A tale of two haplotype groups: The origin and distribution of divergent New World <em>Junonia COI</em> haplotypes</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nucleic Acids Res</em> 45.</journal_title>
                        <volume></volume>
                        <author>Redin D, Borgstrom E, He MX, et al.</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi></doi>
                        <article_title>Droplet Barcode Sequencing for targeted linked-read haplotyping of single DNA molecules</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>BMC Genomics</em></journal_title>
                        <volume>10</volume>
                        <author>Lim J, Kim SY, Kim S, et al.</author>
                        <first_page>S8</first_page>
                        <last_page></last_page>
                        <cYear>2009</cYear>
                        <doi></doi>
                        <article_title>BioBarcode: a general DNA barcoding database and server platform for Asian biodiversity resources</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Genome</em></journal_title>
                        <volume>60</volume>
                        <author>   Bezeng BS, Davies TJ, Daru BH, et al.</author>
                        <first_page>629</first_page>
                        <last_page>638</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1139/gen-2016-0198</doi>
                        <article_title>Ten years of barcoding at the African Centre for DNA Barcoding</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mar Freshwater Res</em></journal_title>
                        <volume>68</volume>
                        <author>   Carew ME, Nichols SJ, Batovska J, et al.</author>
                        <first_page>1788</first_page>
                        <last_page>1802</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1071/MF16304</doi>
                        <article_title>A DNA barcode database of Australia's freshwater macroinvertebrate fauna</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>--Elementary. <em>J Nematol</em></journal_title>
                        <volume>31</volume>
                        <author>   Wheeler QD</author>
                        <first_page>134</first_page>
                        <last_page>141</last_page>
                        <cYear>1999</cYear>
                        <doi></doi>
                        <article_title>Why the phylogenetic species concept</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Am J Bot</em></journal_title>
                        <volume>96</volume>
                        <author>   Spooner DM</author>
                        <first_page>1177</first_page>
                        <last_page>1189</last_page>
                        <cYear>2009</cYear>
                        <doi>10.3732/ajb.0800246</doi>
                        <article_title>DNA barcoding will frequently fail in complicated groups: an example in wild potatoes</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Phil Trans R Soc B</em></journal_title>
                        <volume>360</volume>
                        <author>  DeSalle R, Egan MG, Siddall M</author>
                        <first_page>1905</first_page>
                        <last_page>1916</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1098/rstb.2005.1722</doi>
                        <article_title>The unholy trinity: taxonomy, species delimitation and DNA barcoding</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Syst Biodivers</em></journal_title>
                        <volume>4</volume>
                        <author>   Brower AVZ</author>
                        <first_page>127</first_page>
                        <last_page>132</last_page>
                        <cYear>2006</cYear>
                        <doi>10.1017/S147720000500191X</doi>
                        <article_title>Problems with DNA barcodes for species delimitation: 'ten species' of <em>Astraptes fulgerator</em> reassessed (Lepidoptera: Hesperiidae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Syst Biodivers</em></journal_title>
                        <volume>8</volume>
                        <author>   Brower AVZ</author>
                        <first_page>485</first_page>
                        <last_page>491</last_page>
                        <cYear>2010</cYear>
                        <doi>10.1080/14772000.2010.534512</doi>
                        <article_title>Alleviating the taxonomic impediment of DNA barcoding and setting a bad precedent: names for ten species of '<em>Astraptes fulgerator</em>' (Lepidoptera: Hesperiidae: Eudaminae) with DNA-based diagnoses</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Syst Ent</em></journal_title>
                        <volume>33</volume>
                        <author>   Schmidt BC, Sperling FAH</author>
                        <first_page>613</first_page>
                        <last_page>634</last_page>
                        <cYear>2008</cYear>
                        <doi>10.1111/j.1365-3113.2008.00433.x</doi>
                        <article_title>Widespread decoupling of mtDNA variation and species integrity in <em>Grammia</em> tiger moths (Lepidoptera: Noctuidae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS ONE</em></journal_title>
                        <volume>10</volume>
                        <author>Dupuis JR, Sperling FAH</author>
                        <first_page>e0141882</first_page>
                        <last_page></last_page>
                        <cYear>2015</cYear>
                        <doi>10.1371/journal.pone.0141882</doi>
                        <article_title>Repeated reticulate evolution in North American <em>Papilio machaon</em> group swallowtail butterflies</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>fontinalis</em>) from eastern Quebec, Canada. <em>Mol Ecol</em></journal_title>
                        <volume>7</volume>
                        <author>   Glemet H, Blier P, Bernatchez L</author>
                        <first_page>1655</first_page>
                        <last_page>1662</last_page>
                        <cYear>1998</cYear>
                        <doi></doi>
                        <article_title>Geographical extent of Arctic char (<em>Salvelinus alpinus</em>) mtDNA introgression in brook char populations (<em>S</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Proc Nat Acad Sci USA</em></journal_title>
                        <volume>109</volume>
                        <author>   Stegemann S, Keuthe M, Greiner S, et al.</author>
                        <first_page>2434</first_page>
                        <last_page>2438</last_page>
                        <cYear>2012</cYear>
                        <doi>10.1073/pnas.1114076109</doi>
                        <article_title>Horizontal transfer of chloroplast genomes between plant species</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>Case study in Lamiales <em>Syst Biol</em></journal_title>
                        <volume>54</volume>
                        <author>   Wortley AH, Rudall PJ, Harris DJ, et al.</author>
                        <first_page>697</first_page>
                        <last_page>709</last_page>
                        <cYear>2005</cYear>
                        <doi></doi>
                        <article_title>How much data are needed to resolve a difficult phylogeny</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Syst Ent</em></journal_title>
                        <volume>42</volume>
                        <author>   Peters MJ, Marcus JM</author>
                        <first_page>288</first_page>
                        <last_page>300</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1111/syen.12214</doi>
                        <article_title>Taxonomy as a hypothesis: testing the status of the Bermuda buckeye butterfly <em>Junonia coenia bergi</em> (Lepidoptera: Nymphalidae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Anthropol Anz</em></journal_title>
                        <volume>64</volume>
                        <author>   Wiesemüller B, Rothe H</author>
                        <first_page>161</first_page>
                        <last_page>165</last_page>
                        <cYear>2006</cYear>
                        <doi></doi>
                        <article_title>Interpretation of bootstrap values in phylogenetic analysis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Lepid Soc</em></journal_title>
                        <volume>66</volume>
                        <author>   Pfeiler E, Johnson S, Markow TA</author>
                        <first_page>185</first_page>
                        <last_page>198</last_page>
                        <cYear>2012</cYear>
                        <doi>10.18473/lepi.v66i4.a1</doi>
                        <article_title>DNA barcodes and insights into the relationships and systematics of buckeye butterflies (Nymphalidae: Nymphalinae:<em> Junonia</em>) from the Americas</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Lepid Soc</em></journal_title>
                        <volume>70</volume>
                        <author>   Pfeiler E, Laclette MRL, Markow TA</author>
                        <first_page>85</first_page>
                        <last_page>95</last_page>
                        <cYear>2016</cYear>
                        <doi>10.18473/lepi.70i2.a2</doi>
                        <article_title>Polyphyly in <em>Urbanus</em> and <em>Astraptes</em> (Hesperiidae: Eudaminae) assessed using mitochondrial DNA barcodes, with a reinstated status proposed for <em>Achalarus</em></article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>New Phytol</em></journal_title>
                        <volume>201</volume>
                        <author>   Bock DG, Kane NC, Ebert DP, et al.</author>
                        <first_page>1021</first_page>
                        <last_page>1030</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1111/nph.12560</doi>
                        <article_title>Genome skimming reveals the origin of the Jerusalem Artichoke tuber crop species: neither from Jerusalem nor an artichoke</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Ann Bot</em></journal_title>
                        <volume>117</volume>
                        <author>   Turner B, Paun O, Munzinger J, et al.</author>
                        <first_page>1175</first_page>
                        <last_page>1185</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1093/aob/mcw060</doi>
                        <article_title>Sequencing of whole plastid genomes and nuclear ribosomal DNA of <em>Diospyros</em> species (Ebenaceae) endemic to New Caledonia: many species, little divergence</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Syst Biol</em></journal_title>
                        <volume>64</volume>
                        <author>   Dodsworth S, Chase MW, Kelly LJ, et al.</author>
                        <first_page>112</first_page>
                        <last_page>126</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1093/sysbio/syu080</doi>
                        <article_title>Genomic repeat abundances contain phylogenetic signal</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Biol J Linn Soc</em></journal_title>
                        <volume>117</volume>
                        <author>   Dodsworth S, Chase MW, Sarkinen T, et al.</author>
                        <first_page>96</first_page>
                        <last_page>105</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1111/bij.12612</doi>
                        <article_title>Using genomic repeats for phylogenomics: a case study in wild tomatoes (<em>Solanum</em> section Lycopersicon: Solanaceae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Biol Evol</em></journal_title>
                        <volume>31</volume>
                        <author>   Gillett CPDT, Crampton-Platt A, Timmermans MJTN, et al.</author>
                        <first_page>2223</first_page>
                        <last_page>2237</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1093/molbev/msu154</doi>
                        <article_title>Bulk de novo mitogenome assembly from pooled total DNA elucidates the phylogeny of weevils (Coleoptera: Curculionoidea)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>High-throughput multiplex sequencing of mitochondrial genomes for molecular systematics. <em>Nucleic Acids Res</em></journal_title>
                        <volume>38</volume>
                        <author>Timmermans MJTN, Dodsworth S, Culverwell CL, et al.</author>
                        <first_page>e197</first_page>
                        <last_page></last_page>
                        <cYear>2010</cYear>
                        <doi></doi>
                        <article_title>Why barcode</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Phylogen Evol</em></journal_title>
                        <volume>79</volume>
                        <author>   Timmermans MJTN, Lees DC, Simonsen TJ</author>
                        <first_page>169</first_page>
                        <last_page>178</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1016/j.ympev.2014.05.031</doi>
                        <article_title>Towards a mitogenomic phylogeny of Lepidoptera</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em> BMC Genomics</em></journal_title>
                        <volume>15</volume>
                        <author>Wu LW, Lin LH, Lees D, et al.</author>
                        <first_page>468</first_page>
                        <last_page></last_page>
                        <cYear>2014</cYear>
                        <doi>10.1186/1471-2164-15-468</doi>
                        <article_title>Mitogenomic sequences effectively recover relationships within brush-footed butterflies (Lepidoptera: Nymphalidae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLOS One</em></journal_title>
                        <volume>10</volume>
                        <author>Shi QH, Sun XY, Wang YL, et al.</author>
                        <first_page>e0124349</first_page>
                        <last_page></last_page>
                        <cYear>2015</cYear>
                        <doi>10.1371/journal.pone.0124349</doi>
                        <article_title>Morphological characters are compatible with mitogenomic data in resolving the phylogeny of Nymphalid butterflies (Lepidoptera: Papilionoidea: Nymphalidae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>Available from: http://www.genome.gov/sequencingcostsdata.</journal_title>
                        <volume></volume>
                        <author>Wetterstrand KA</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2018</cYear>
                        <doi></doi>
                        <article_title>DNA sequencing costs: Data from the NHGRI Genome Sequencing Program (GSP)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Syst Ent</em></journal_title>
                        <volume>39</volume>
                        <author>   Borchers TE, Marcus JM</author>
                        <first_page>242</first_page>
                        <last_page>255</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1111/syen.12053</doi>
                        <article_title>Genetic population structure of buckeye butterflies (<em>Junonia</em>) from Argentina</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Psyche</em></journal_title>
                        <volume>2014</volume>
                        <author>   Gemmell AP, Borchers TE, Marcus JM</author>
                        <first_page>1</first_page>
                        <last_page>21</last_page>
                        <cYear>2014</cYear>
                        <doi></doi>
                        <article_title>Genetic population structure of buckeye butterflies (<em>Junonia</em>) from French Guiana, Martinique, and Guadeloupe</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Evol Dev</em></journal_title>
                        <volume>17</volume>
                        <author>   Abbasi R, Marcus JM</author>
                        <first_page>63</first_page>
                        <last_page>81</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1111/ede.12109</doi>
                        <article_title>Color pattern evolution in <em>Vanessa</em> butterflies (Nymphalidae: Nymphalini): Non-eyespot characters</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Ann Entomol Soc Am</em></journal_title>
                        <volume>68</volume>
                        <author>   Wallace JB</author>
                        <first_page>463</first_page>
                        <last_page>472</last_page>
                        <cYear>1975</cYear>
                        <doi>10.1093/aesa/68.3.463</doi>
                        <article_title>Food partitioning in net-spinning trichoptera larvae: <em>Hydropsyche venularis</em>, <em>Cheumatopsyche etrona</em>, and <em>Maconema zebratum</em> (Hydropsychidae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Zool Scr</em></journal_title>
                        <volume>31</volume>
                        <author>   Kjer KM, Blahnik RJ, Holzenthal RW</author>
                        <first_page>83</first_page>
                        <last_page>91</last_page>
                        <cYear>2002</cYear>
                        <doi>10.1046/j.0300-3256.2001.00079.x</doi>
                        <article_title>Phylogeny of caddisflies (Insecta, Trichoptera)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>BMC Ecology</em></journal_title>
                        <volume>13</volume>
                        <author>Ruiter DE, Boyle EE, Zhou X</author>
                        <first_page>5</first_page>
                        <last_page></last_page>
                        <cYear>2013</cYear>
                        <doi>10.1186/1472-6785-13-5</doi>
                        <article_title>DNA barcoding facilitates associations and diagnoses for Trichoptera larvae of the Churchill (Manitoba, Canada) area</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Syst Biol</em></journal_title>
                        <volume>50</volume>
                        <author>   Kjer KM, Blahnik RJ, Holzenthal RW</author>
                        <first_page>781</first_page>
                        <last_page>816</last_page>
                        <cYear>2001</cYear>
                        <doi>10.1080/106351501753462812</doi>
                        <article_title>Phylogeny of Trichoptera (Caddisflies): Characterization of signal and noise within multiple datasets</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Phil Trans R Soc B</em></journal_title>
                        <volume>371</volume>
                        <author>Zhou X, Frandsen PB, Holzenthal RW, et al.</author>
                        <first_page>20160025</first_page>
                        <last_page></last_page>
                        <cYear>2016</cYear>
                        <doi>10.1098/rstb.2016.0025</doi>
                        <article_title>The Trichoptera barcode initiative: a strategy for generating a species-level Tree of Life</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>BMC Evol Biol</em></journal_title>
                        <volume>14</volume>
                        <author>Peters RS, Meusemann K, Petersen M, et al.</author>
                        <first_page>52</first_page>
                        <last_page></last_page>
                        <cYear>2014</cYear>
                        <doi>10.1186/1471-2148-14-52</doi>
                        <article_title>The evolutionary history of holometabolous insects inferred from transcriptome-based phylogeny and comprehensive morphological data</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Sci Rep</em></journal_title>
                        <volume>7</volume>
                        <author>Abbasi R, Marcus JM</author>
                        <first_page>16337</first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi>10.1038/s41598-017-16553-5</doi>
                        <article_title>A new A-P compartment boundary and organizer in holometabolous insect wings</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>Los Angeles, CA: The Lepidopterists' Society. 444 p.</journal_title>
                        <volume></volume>
                        <author>Winter WD</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2000</cYear>
                        <doi></doi>
                        <article_title>Basic Techniques for Observing and Studying Moths and Butterflies; Miller WE, editor</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mitochondrial DNA B Resour</em></journal_title>
                        <volume>2</volume>
                        <author>   Living Prairie Mitogenomics Consortium</author>
                        <first_page>344</first_page>
                        <last_page>346</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1080/23802359.2017.1334525</doi>
                        <article_title>The complete mitochondrial genome of the lesser aspen webworm moth <em>Meroptera pravella</em> (Insecta: Lepidoptera: Pyralidae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mitochondrial DNA B Resour</em></journal_title>
                        <volume>2</volume>
                        <author>   Peirson DSJ, Marcus JM</author>
                        <first_page>595</first_page>
                        <last_page>597</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1080/23802359.2017.1372728</doi>
                        <article_title>The complete mitochondrial genome of the North American caddisfly <em>Anabolia bimaculata</em> (Insecta: Trichoptera: Limnephilidae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mitochondrial DNA B Resour</em></journal_title>
                        <volume>2</volume>
                        <author>   Lalonde MLM, Marcus JM</author>
                        <first_page>765</first_page>
                        <last_page>767</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1080/23802359.2017.1398619</doi>
                        <article_title>The complete mitochondrial genome of the long-horned caddisfly <em>Triaenodes tardus</em> (Insecta: Trichoptera: Leptoceridae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Zool Syst</em></journal_title>
                        <volume>40</volume>
                        <author>   McCullagh BS, Wissinger SA, Marcus JM</author>
                        <first_page>459</first_page>
                        <last_page>469</last_page>
                        <cYear>2015</cYear>
                        <doi></doi>
                        <article_title>Identifying PCR primers to facilitate molecular phylogenetics in Caddisflies (order Trichoptera)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Bioinformatics</em></journal_title>
                        <volume>28</volume>
                        <author>   Kearse M, Moir R, Wilson A, et al.</author>
                        <first_page>1647</first_page>
                        <last_page>1649</last_page>
                        <cYear>2012</cYear>
                        <doi>10.1093/bioinformatics/bts199</doi>
                        <article_title>Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>BMC Bioinformatics</em></journal_title>
                        <volume>11</volume>
                        <author>Reuter JS, Mathews DH</author>
                        <first_page>129</first_page>
                        <last_page></last_page>
                        <cYear>2010</cYear>
                        <doi>10.1186/1471-2105-11-129</doi>
                        <article_title>RNAstructure: software for RNA secondary structure prediction and analysis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nucleic Acids Res</em></journal_title>
                        <volume>31</volume>
                        <author>   Zuker M</author>
                        <first_page>3406</first_page>
                        <last_page>3415</last_page>
                        <cYear>2003</cYear>
                        <doi>10.1093/nar/gkg595</doi>
                        <article_title>Mfold web server for nucleic acid folding and hybridization prediction</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Int J Biol Scii</em></journal_title>
                        <volume>10</volume>
                        <author>   Wang Y, Liu X, Yang D</author>
                        <first_page>53</first_page>
                        <last_page>63</last_page>
                        <cYear>2014</cYear>
                        <doi>10.7150/ijbs.7975</doi>
                        <article_title>The first mitochondrial genome for caddisfly (Insecta: Trichoptera) with phylogenetic implications</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>3 kb silkworm <em>Attacus ricini</em> rDNA repeat, determination of the transcriptional initiation site and functional analysis of the intergenic spacer. <em>DNA Sequence</em></journal_title>
                        <volume>14</volume>
                        <author>   Wang SQ, Zhao MJ, Li TP</author>
                        <first_page>95</first_page>
                        <last_page>101</last_page>
                        <cYear>2003</cYear>
                        <doi></doi>
                        <article_title>Complete sequence of the 10</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mitochondrial DNA A DNA Mapp Seq Anal</em></journal_title>
                        <volume>28</volume>
                        <author>   Linard B, Arribas P, Andujar C, et al.</author>
                        <first_page>71</first_page>
                        <last_page>72</last_page>
                        <cYear>2017</cYear>
                        <doi></doi>
                        <article_title>The mitogenome of <em>Hydropsyche pellucidula</em> (Hydropsychidae): First gene arrangement in the insect order Trichoptera</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mitochondrial DNA A DNA Mapp Seq Anal</em></journal_title>
                        <volume>27</volume>
                        <author>   Dietz L, Brand P, Eschner LM, et al.</author>
                        <first_page>3293</first_page>
                        <last_page>3294</last_page>
                        <cYear>2015</cYear>
                        <doi></doi>
                        <article_title>The mitochondrial genomes of the caddisflies <em>Sericostoma personatum</em> and <em>Thremma gallicum</em> (Insecta: Trichoptera)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Syst Biol</em></journal_title>
                        <volume>7</volume>
                        <author>Sievers F, Wilm A, Dineen D, et al.</author>
                        <first_page>539</first_page>
                        <last_page></last_page>
                        <cYear>2011</cYear>
                        <doi></doi>
                        <article_title>Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Asia Pac Entomol</em></journal_title>
                        <volume>15</volume>
                        <author>   Kim JS, Park JS, Kim MJ, et al.</author>
                        <first_page>162</first_page>
                        <last_page>173</last_page>
                        <cYear>2012</cYear>
                        <doi>10.1016/j.aspen.2011.10.002</doi>
                        <article_title>Complete nucleotide sequence and organization of the mitochondrial genome of eri-silkworm, <em>Samia cynthia ricini</em> (Lepidoptera: Saturniidae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sunderland, Massachusetts, USA: Sinauer Associates.</journal_title>
                        <volume></volume>
                        <author>Swofford DL</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2002</cYear>
                        <doi></doi>
                        <article_title>PAUP*</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nat Methods</em></journal_title>
                        <volume>9</volume>
                        <author>Darriba D, Taboada GL, Doallo R, et al.</author>
                        <first_page>772</first_page>
                        <last_page></last_page>
                        <cYear>2012</cYear>
                        <doi></doi>
                        <article_title>jModelTest 2: more models, new heuristics and parallel computing</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Science</em></journal_title>
                        <volume>276</volume>
                        <author>   Huelsenbeck JP, Rannala B</author>
                        <first_page>227</first_page>
                        <last_page>232</last_page>
                        <cYear>1997</cYear>
                        <doi>10.1126/science.276.5310.227</doi>
                        <article_title>Phylogenetic methods come of age: Testing hypotheses in an evolutionary context</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Tinea</em></journal_title>
                        <volume>23</volume>
                        <author>   Ishizuka K, Matsuo M, Nonaka M</author>
                        <first_page>157</first_page>
                        <last_page>170</last_page>
                        <cYear>2015</cYear>
                        <doi></doi>
                        <article_title>Molecular phylogenetic analysis of <em>Catocala</em> moths based on the nuclear ITS2 and 28S rRNA gene sequences (Lepidoptera, Noctuidae)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Parasitol Int</em></journal_title>
                        <volume>65</volume>
                        <author>   Briscoe AG, Bray RA, Brabec J, et al.</author>
                        <first_page>271</first_page>
                        <last_page>275</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1016/j.parint.2016.02.004</doi>
                        <article_title>The mitochondrial genome and ribosomal operon of <em>Brachycladium goliath</em> (Digenea: Brachycladiidae) recovered from a stranded minke whale</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Annu Rev Entomol</journal_title>
                        <volume></em>59</volume>
                        <author>   Cameron SL</author>
                        <first_page>95</first_page>
                        <last_page>117</last_page>
                        <cYear>2014</cYear>
                        <doi></doi>
                        <article_title>Insect Mitochondrial Genomics: Implications for Evolution and Phylogeny</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em> J N Amer Benthol Soc</em></journal_title>
                        <volume>29</volume>
                        <author>   Geraci CJ, Zhou X, Morse JC, et al.</author>
                        <first_page>918</first_page>
                        <last_page>933</last_page>
                        <cYear>2010</cYear>
                        <doi>10.1899/09-031.1</doi>
                        <article_title>Defining the genus <em>Hydropsyche</em> (Trichoptera:Hydropsychidae) based on DNA and morphological evidence</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Am Nat</em></journal_title>
                        <volume>180</volume>
                        <author>   Irwin DE</author>
                        <first_page>35</first_page>
                        <last_page>49</last_page>
                        <cYear>2012</cYear>
                        <doi>10.1086/666002</doi>
                        <article_title>Local adaptation along smooth ecological gradients causes phylogeographic breaks and phenotypic clustering</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Syst Evol</em></journal_title>
                        <volume>46</volume>
                        <author>   Heath TA, Hedtke SM, Hillis DM</author>
                        <first_page>239</first_page>
                        <last_page>257</last_page>
                        <cYear>2008</cYear>
                        <doi></doi>
                        <article_title>Taxon sampling and the accuracy of phylogenetic analysis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Phil Trans Roy Soc B</em></journal_title>
                        <volume>360</volume>
                        <author>   Janzen DH, Hajibabaei M, Burns JM, et al.</author>
                        <first_page>1835</first_page>
                        <last_page>1845</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1098/rstb.2005.1715</doi>
                        <article_title>Wedding biodiversity inventory of a large and complex Lepidoptera fauna with DNA barcoding</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>montanana</em> (Walsingham 1884) (Tortricidae: Olethreutinae) in Western Canada using morphology and DNA. <em>J Lepid Soc</em></journal_title>
                        <volume>67</volume>
                        <author>   Jaeger CM, Dombroskie JJ, Sperling FAH</author>
                        <first_page>253</first_page>
                        <last_page>262</last_page>
                        <cYear>2013</cYear>
                        <doi></doi>
                        <article_title>Delimitation of <em>Phaneta taradana</em> (Moschler 1874) and <em>P</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>BMC Evol Biol</em> 15.</journal_title>
                        <volume></volume>
                        <author>Proshek B, Dupuis JR, Engberg A, et al.</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2015</cYear>
                        <doi></doi>
                        <article_title>Genetic evaluation of the evolutionary distinctness of a federally endangered butterfly, Lange's Metalmark</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>BMC Evol Biol</em></journal_title>
                        <volume>9</volume>
                        <author>Wahlberg N, Weingartner E, Warren A, et al.</author>
                        <first_page>92</first_page>
                        <last_page></last_page>
                        <cYear>2009</cYear>
                        <doi>10.1186/1471-2148-9-92</doi>
                        <article_title>Timing major conflict between mitochondrial and nuclear genes in species relationships of <em>Polygonia</em> butterflies (Nymphalidae: Nymphalini)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Ecol Evol</em></journal_title>
                        <volume>3</volume>
                        <author>   Kodandaramaiah U, Simonsen TJ, Bromilow S, et al.</author>
                        <first_page>5167</first_page>
                        <last_page>5176</last_page>
                        <cYear>2013</cYear>
                        <doi>10.1002/ece3.886</doi>
                        <article_title>Deceptive single-locus taxonomy and phylogeography: <em>Wolbachia</em>-associated divergence in mitochondrial DNA is not reflected in morphology and nuclear markers in a butterfly species</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Trends Plant Sci</em></journal_title>
                        <volume>20</volume>
                        <author>   Dodsworth S</author>
                        <first_page>525</first_page>
                        <last_page>527</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1016/j.tplants.2015.06.012</doi>
                        <article_title>Genome skimming for next-generation biodiversity analysis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nature</em></journal_title>
                        <volume>331</volume>
                        <author>   Lake JA</author>
                        <first_page>184</first_page>
                        <last_page>186</last_page>
                        <cYear>1988</cYear>
                        <doi>10.1038/331184a0</doi>
                        <article_title>Origin of the eukaryotic nucleus by rate-invariant analyisis of rRNA sequences</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Zookeys</em>: 129–141.</journal_title>
                        <volume></volume>
                        <author>Wahlberg N, Pena C, Ahola M, et al.</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2016</cYear>
                        <doi></doi>
                        <article_title>PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLOS ONE</em></journal_title>
                        <volume>11</volume>
                        <author>Maricic T, Whitten M, Pääbo S</author>
                        <first_page>e14004</first_page>
                        <last_page></last_page>
                        <cYear>2010</cYear>
                        <doi></doi>
                        <article_title>Multiplexed DNA sequence capture of mitochondrial genomes using PCR products</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Syst Biol</em>: syx048.</journal_title>
                        <volume></volume>
                        <author>Breinholt JW, Earl C, Lemmon AR, et al.</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi></doi>
                        <article_title>Resolving relationships among the megadiverse butterflies and moths with a novel pipeline for anchored phylogenomics</article_title>
                    </citation>
                                   </citation_list>
                <keywords language="en"></keywords>
				<abstract language="en"><![CDATA[DNA barcodes are very useful for species identification especially when identification by traditional morphological characters is difficult. However, the short mitochondrial and chloroplast barcodes currently in use often fail to distinguish between closely related species, are prone to lateral transfer, and provide inadequate phylogenetic resolution, particularly at deeper nodes. The deficiencies of short barcode identifiers are similar to the deficiencies of the short year identifiers that caused the Y2K problem in computer science. The resolution of the Y2K problem was to increase the size of the year identifiers. The performance of conventional mitochondrial <em>COI</em> barcodes for phylogenetics was compared with the performance of complete mitochondrial genomes and nuclear ribosomal RNA repeats obtained by genome skimming for a set of caddisfly taxa (Insect Order Trichoptera). The analysis focused on Trichoptera Family Hydropsychidae, the net-spinning caddisflies, which demonstrates many of the frustrating limitations of current barcodes. To conduct phylogenetic comparisons, complete mitochondrial genomes (15 kb each) and nuclear ribosomal repeats (9 kb each) from six caddisfly species were sequenced, assembled, and are reported for the first time. These sequences were analyzed in comparison with eight previously published trichopteran mitochondrial genomes and two triochopteran <em>rRNA</em> repeats, plus outgroup sequences from sister clade Lepidoptera (butterflies and moths). <em>COI</em> trees were not well-resolved, had low bootstrap support, and differed in topology from prior phylogenetic analyses of the Trichoptera. Phylogenetic trees based on mitochondrial genomes or <em>rRNA</em> repeats were well-resolved with high bootstrap support and were largely congruent with each other. Because they are easily sequenced by genome skimming, provide robust phylogenetic resolution at various phylogenetic depths, can better distinguish between closely related species, and (in the case of mitochondrial genomes), are backwards compatible with existing mitochondrial barcodes, it is proposed that mitochondrial genomes and <em>rRNA</em> repeats be used as next generation DNA barcodes.]]></abstract>
			</journal_article>
                        <journal_article>
	            	            <titles>
                    <title><![CDATA[Expression characterization and functional implication of the collagen-modifying Leprecan proteins in mouse gonadal tissue and mature sperm]]></title>
                </titles>
	                            <contributors>
                                </contributors>
                <publication_date media_type="print">
                    <month></month>
                    <year>2018</year>
                </publication_date>
                
                <pages>
                    <first_page>24</first_page>
                    <last_page>40</last_page>
                </pages>
                <doi_data>
                    <doi>10.3934/genet.2018.1.24</doi>
                    <timestamp>2017-10-05 12:00:00.0</timestamp>
					<resource>http://www.aimsciences.org/article/id/1823</resource>
                </doi_data>
	                
                <citation_list>
               	                    <citation key="">
                        <journal_title><em>Cell</em></journal_title>
                        <volume>127</volume>
                        <author>     Morello R, Bertin TK, Chen Y, et al.</author>
                        <first_page>291</first_page>
                        <last_page>304</last_page>
                        <cYear>2006</cYear>
                        <doi>10.1016/j.cell.2006.08.039</doi>
                        <article_title>CRTAP is required for prolyl 3- hydroxylation and mutations cause recessive osteogenesis imperfecta</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nat Genet</em></journal_title>
                        <volume>39</volume>
                        <author>     Cabral WA, Chang W, Barnes AM, et al.</author>
                        <first_page>359</first_page>
                        <last_page>365</last_page>
                        <cYear>2007</cYear>
                        <doi>10.1038/ng1968</doi>
                        <article_title>Prolyl 3-hydroxylase 1 deficiency causes a recessive metabolic bone disorder resembling lethal/severe osteogenesis imperfecta</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Am J Hum Genet</em></journal_title>
                        <volume>85</volume>
                        <author>     van Dijk FS, Nesbitt IM, Zwikstra EH, et al.</author>
                        <first_page>521</first_page>
                        <last_page>527</last_page>
                        <cYear>2009</cYear>
                        <doi>10.1016/j.ajhg.2009.09.001</doi>
                        <article_title>PPIB mutations cause severe osteogenesis imperfecta</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Am J Hum Genet</em></journal_title>
                        <volume>89</volume>
                        <author>     Mordechai S, Gradstein L, Pasanen A, et al.</author>
                        <first_page>438</first_page>
                        <last_page>445</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1016/j.ajhg.2011.08.003</doi>
                        <article_title>High myopia caused by a mutation in LEPREL1, encoding prolyl 3-hydroxylase 2</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em> J Biol Chem</em></journal_title>
                        <volume>290</volume>
                        <author>     Hudson DM, Joeng KS, Werther R, et al.</author>
                        <first_page>8613</first_page>
                        <last_page>8622</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1074/jbc.M114.634915</doi>
                        <article_title>Post-translationally abnormal collagens of prolyl 3-hydroxylase-2 null mice offer a pathobiological mechanism for the high myopia linked to human LEPREL1 mutations</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Biochem Cell Biol</em></journal_title>
                        <volume>70</volume>
                        <author>     Chen Q, Pearlman RE, Moens PB</author>
                        <first_page>1030</first_page>
                        <last_page>1038</last_page>
                        <cYear>1992</cYear>
                        <doi>10.1139/o92-147</doi>
                        <article_title>Isolation and characterization of a cDNA encoding a synaptonemal complex protein</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Exp Cell Res</em></journal_title>
                        <volume>318</volume>
                        <author>     Fraune J, Schramm S, Alsheimer M, et al.</author>
                        <first_page>1340</first_page>
                        <last_page>1346</last_page>
                        <cYear>2012</cYear>
                        <doi>10.1016/j.yexcr.2012.02.018</doi>
                        <article_title>The mammalian synaptonemal complex: protein components, assembly and role in meiotic recombination</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Br  J Cancer</em></journal_title>
                        <volume>83</volume>
                        <author>     Fossa A, Siebert R, Aasheim HC, et al.</author>
                        <first_page>743</first_page>
                        <last_page>749</last_page>
                        <cYear>2000</cYear>
                        <doi>10.1054/bjoc.2000.1365</doi>
                        <article_title>Identification of nucleolar protein No55 as a tumour-associated autoantigen in patients with prostate cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J cell Biol</em></journal_title>
                        <volume>177</volume>
                        <author>     Gaboreanu AM, Hrstka R, Xu W, et al.</author>
                        <first_page>707</first_page>
                        <last_page>716</last_page>
                        <cYear>2007</cYear>
                        <doi>10.1083/jcb.200608060</doi>
                        <article_title>Myelin protein zero/P0 phosphorylation and function require an adaptor protein linking it to RACK1 and PKC alpha</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Physiol  Behav</em></journal_title>
                        <volume>98</volume>
                        <author>   Romcy-Pereira RN, Erraji-Benchekroun L, Smyrniotopoulos P, et al.</author>
                        <first_page>44</first_page>
                        <last_page>52</last_page>
                        <cYear>2009</cYear>
                        <doi>10.1016/j.physbeh.2009.04.010</doi>
                        <article_title>Sleep-dependent gene expression in the hippocampus and prefrontal cortex following long-term potentiation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Proteomics</em></journal_title>
                        <volume>5</volume>
                        <author>   Crockett DK, Fillmore GC, Elenitoba-Johnson KS, et al.</author>
                        <first_page>1250</first_page>
                        <last_page>1262</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1002/pmic.200401046</doi>
                        <article_title>Analysis of phosphatase and tensin homolog tumor suppressor interacting proteins by in vitro and in silico proteomics</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Bone Mineral Res</journal_title>
                        <volume></em>29</volume>
                        <author>   Gruenwald K, Castagnola P, Besio R, et al.</author>
                        <first_page>666</first_page>
                        <last_page>675</last_page>
                        <cYear>2014</cYear>
                        <doi></doi>
                        <article_title>Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS Genet</em></journal_title>
                        <volume>12</volume>
                        <author>Heard ME, Besio R, Weis M, et al.</author>
                        <first_page>e1006002</first_page>
                        <last_page></last_page>
                        <cYear>2016</cYear>
                        <doi>10.1371/journal.pgen.1006002</doi>
                        <article_title>Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J B iol Chem</em></journal_title>
                        <volume>292</volume>
                        <author>   Hudson DM, Weis M, Rai J, et al.</author>
                        <first_page>3877</first_page>
                        <last_page>3887</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1074/jbc.M116.762245</doi>
                        <article_title>P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PloS one</em></journal_title>
                        <volume>5</volume>
                        <author>Baldridge D, Lennington J, Weis M, et al.</author>
                        <first_page>e10560</first_page>
                        <last_page></last_page>
                        <cYear>2010</cYear>
                        <doi>10.1371/journal.pone.0010560</doi>
                        <article_title>Generalized connective tissue disease in Crtap-/- mouse</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Hum M ol Genet</em></journal_title>
                        <volume>19</volume>
                        <author>   Chang W, Barnes AM, Cabral WA, et al.</author>
                        <first_page>223</first_page>
                        <last_page>234</last_page>
                        <cYear>2010</cYear>
                        <doi>10.1093/hmg/ddp481</doi>
                        <article_title>Prolyl 3-hydroxylase 1 and CRTAP are mutually stabilizing in the endoplasmic reticulum collagen prolyl 3-hydroxylation complex</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J  Cell Biol</em></journal_title>
                        <volume>100</volume>
                        <author>   Skinner MK, Tung PS, Fritz IB</author>
                        <first_page>1941</first_page>
                        <last_page>1947</last_page>
                        <cYear>1985</cYear>
                        <doi>10.1083/jcb.100.6.1941</doi>
                        <article_title>Cooperativity between Sertoli cells and testicular peritubular cells in the production and deposition of extracellular matrix components</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cell Tissue Res</em></journal_title>
                        <volume>339</volume>
                        <author>   Irving-Rodgers HF, Hummitzsch K, Murdiyarso LS, et al.</author>
                        <first_page>613</first_page>
                        <last_page>624</last_page>
                        <cYear>2010</cYear>
                        <doi>10.1007/s00441-009-0905-8</doi>
                        <article_title>Dynamics of extracellular matrix in ovarian follicles and corpora lutea of mice</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J  Cell Biol</em></journal_title>
                        <volume>173</volume>
                        <author>   Yang F, De La Fuente R, Leu NA, et al.</author>
                        <first_page>497</first_page>
                        <last_page>507</last_page>
                        <cYear>2006</cYear>
                        <doi>10.1083/jcb.200603063</doi>
                        <article_title>Mouse SYCP2 is required for synaptonemal complex assembly and chromosomal synapsis during male meiosis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell</em></journal_title>
                        <volume>5</volume>
                        <author>   Yuan L, Liu JG, Zhao J, et al.</author>
                        <first_page>73</first_page>
                        <last_page>83</last_page>
                        <cYear>2000</cYear>
                        <doi>10.1016/S1097-2765(00)80404-9</doi>
                        <article_title>The murine SCP3 gene is required for synaptonemal complex assembly, chromosome synapsis, and male fertility</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Genes  Dev</em></journal_title>
                        <volume>19</volume>
                        <author>   de Vries FA, de Boer E, van den Bosch M, et al.</author>
                        <first_page>1376</first_page>
                        <last_page>1389</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1101/gad.329705</doi>
                        <article_title>Mouse Sycp1 functions in synaptonemal complex assembly, meiotic recombination, and XY body formation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS Genet</em></journal_title>
                        <volume>5</volume>
                        <author>Bolcun-Filas E, Hall E, Speed R, et al.</author>
                        <first_page>e1000393</first_page>
                        <last_page></last_page>
                        <cYear>2009</cYear>
                        <doi>10.1371/journal.pgen.1000393</doi>
                        <article_title>Mutation of the mouse Syce1 gene disrupts synapsis and suggests a link between synaptonemal complex structural components and DNA repair</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em> J  Cell Biol</em></journal_title>
                        <volume>176</volume>
                        <author>   Bolcun-Filas E, Costa Y, Speed R, et al.</author>
                        <first_page>741</first_page>
                        <last_page>747</last_page>
                        <cYear>2007</cYear>
                        <doi>10.1083/jcb.200610027</doi>
                        <article_title>SYCE2 is required for synaptonemal complex assembly, double strand break repair, and homologous recombination</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS Genet</em></journal_title>
                        <volume>7</volume>
                        <author>Schramm S, Fraune J, Naumann R, et al.</author>
                        <first_page>e1002088</first_page>
                        <last_page></last_page>
                        <cYear>2011</cYear>
                        <doi>10.1371/journal.pgen.1002088</doi>
                        <article_title>A novel mouse synaptonemal complex protein is essential for loading of central element proteins, recombination, and fertility</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J  Cell Sci</em></journal_title>
                        <volume>121</volume>
                        <author>   Hamer G, Wang H, Bolcun-Filas E, et al.</author>
                        <first_page>2445</first_page>
                        <last_page>2451</last_page>
                        <cYear>2008</cYear>
                        <doi>10.1242/jcs.033233</doi>
                        <article_title>Progression of meiotic recombination requires structural maturation of the central element of the synaptonemal complex</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Reproduction</em></journal_title>
                        <volume>130</volume>
                        <author>   He Z, Feng L, Zhang X, et al.</author>
                        <first_page>333</first_page>
                        <last_page>341</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1530/rep.1.00694</doi>
                        <article_title>Expression of Col1a1, Col1a2 and procollagen I in germ cells of immature and adult mouse testis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Chem Mutagens</em>, 257–285.</journal_title>
                        <volume></volume>
                        <author>Wyrobeck AJaB, W. Robert</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>1978</cYear>
                        <doi></doi>
                        <article_title>The Induction of Sperm-Shape Abnormalities in Mice and Humans</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J  Reproduction Dev</em></journal_title>
                        <volume>52</volume>
                        <author>   Kawai Y, Hata T, Suzuki O, et al.</author>
                        <first_page>561</first_page>
                        <last_page>568</last_page>
                        <cYear>2006</cYear>
                        <doi>10.1262/jrd.18023</doi>
                        <article_title>The relationship between sperm morphology and in vitro fertilization ability in mice</article_title>
                    </citation>
                                   </citation_list>
                <keywords language="en"></keywords>
				<abstract language="en"><![CDATA[The Leprecan protein family which includes the prolyl 3-hydroxylase enzymes (P3H1, P3H2, and P3H3), the closely related cartilage-associated protein (CRTAP), and SC65 (Synaptonemal complex 65, aka P3H4, LEPREL4), is involved in the post-translational modification of fibrillar collagens. Mutations in <em>CRTAP</em>, <em>P3H1</em> and <em>P3H2</em> cause human genetic diseases. We recently showed that SC65 forms a stable complex in the endoplasmic reticulum with P3H3 and lysyl hydroxylase 1 and that loss of this complex leads to defective collagen lysyl hydroxylation and causes low bone mass and skin fragility. Interestingly, SC65 was initially described as a synaptonemal complex-associated protein, suggesting a potential additional role in germline cells. In the present study, we describe the expression of SC65, CRTAP and other Leprecan proteins in postnatal mouse reproductive organs. We detect SC65 expression in peritubular cells of testis up to 4 weeks of age but not in cells within seminiferous tubules, while its expression is maintained in ovarian follicles until adulthood. Similar to bone and skin, SC65 and P3H3 are also tightly co-expressed in testis and ovary. Moreover, we show that CRTAP, a protein normally involved in collagen prolyl 3-hydroxylation, is highly expressed in follicles and stroma of the ovary and in testes interstitial cells at 4 weeks of age, germline cells and mature sperm. Importantly, <em>CrtapKO</em> mice have a mild but significant increase in morphologically abnormal mature sperm (17% increase compared to WT). These data suggest a role for the Leprecans in the post-translational modification of collagens expressed in the stroma of the reproductive organs. While we could not confirm that SC65 is part of the synaptonemal complex, the expression of CRTAP in the seminiferous tubules and in mature sperm suggest a role in the testis germ cell lineage and sperm morphogenesis.]]></abstract>
			</journal_article>
                        <journal_article>
	            	            <titles>
                    <title><![CDATA[Time-dependent modulation of FoxO activity by HDAC inhibitor in oncogene-transformed E1A+Ras cells]]></title>
                </titles>
	                            <contributors>
                                </contributors>
                <publication_date media_type="print">
                    <month></month>
                    <year>2018</year>
                </publication_date>
                
                <pages>
                    <first_page>41</first_page>
                    <last_page>52</last_page>
                </pages>
                <doi_data>
                    <doi>10.3934/genet.2018.1.41</doi>
                    <timestamp>2017-11-01 12:00:00.0</timestamp>
					<resource>http://www.aimsciences.org/article/id/1825</resource>
                </doi_data>
	                
                <citation_list>
               	                    <citation key="">
                        <journal_title><em>Oncogene</em></journal_title>
                        <volume>24</volume>
                        <author>     Greer EL, Brunet A</author>
                        <first_page>7410</first_page>
                        <last_page>7425</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1038/sj.onc.1209086</doi>
                        <article_title>FOXO transcription factors at the interface between longevity and tumor suppression</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Redox Biol</em></journal_title>
                        <volume>6</volume>
                        <author>     Klotz L, Sánchez-ramos C, Prieto-arroyo I, et al.</author>
                        <first_page>51</first_page>
                        <last_page>72</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1016/j.redox.2015.06.019</doi>
                        <article_title>Redox regulation of FoxO transcription factors</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Cell Sci</em></journal_title>
                        <volume>127</volume>
                        <author>     Beharry A, Sandesara P, Roberts B, et al.</author>
                        <first_page>1441</first_page>
                        <last_page>1453</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1242/jcs.136390</doi>
                        <article_title>HDAC1 activates FoxO and is both sufficient and required for skeletal muscle atrophy</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Oncogene</em></journal_title>
                        <volume>31</volume>
                        <author>     Wang F, Chan C, Chen K, et al.</author>
                        <first_page>1546</first_page>
                        <last_page>1557</last_page>
                        <cYear>2011</cYear>
                        <doi></doi>
                        <article_title>Deacetylation of FOXO3 by SIRT1 or SIRT2 leads to Skp2-mediated FOXO3 ubiquitination and degradation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Antioxid Redox Signal</em></journal_title>
                        <volume>7</volume>
                        <author>     Furukawa-Hibi Y, Kobayashi Y, Chen C, et al.</author>
                        <first_page>752</first_page>
                        <last_page>760</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1089/ars.2005.7.752</doi>
                        <article_title>FOXO transcription factors in cell-cycle regulation and the response to oxidative stress</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Cell Sci</em></journal_title>
                        <volume>120</volume>
                        <author>     Huang H, Tindall D</author>
                        <first_page>2479</first_page>
                        <last_page>2487</last_page>
                        <cYear>2007</cYear>
                        <doi>10.1242/jcs.001222</doi>
                        <article_title>Dynamic FoxO transcription factors</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Oncogene</em></journal_title>
                        <volume>27</volume>
                        <author>     Calnan В, Brunet A</author>
                        <first_page>2276</first_page>
                        <last_page>2288</last_page>
                        <cYear>2008</cYear>
                        <doi>10.1038/onc.2008.21</doi>
                        <article_title>The FoxO code</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cancer Res</em></journal_title>
                        <volume>71</volume>
                        <author>     Su J, Cheng X, Yamaguchi H, et al.</author>
                        <first_page>6878</first_page>
                        <last_page>6888</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1158/0008-5472.CAN-11-0295</doi>
                        <article_title>FOXO3a-dependent mechanism of E1A-induced chemosensitization</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Trends Biochem Sci</em></journal_title>
                        <volume>30</volume>
                        <author>     van der Heide L, Smidt M</author>
                        <first_page>81</first_page>
                        <last_page>86</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1016/j.tibs.2004.12.002</doi>
                        <article_title>Regulation of FoxO activity by CBP/p300-mediated acetylation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cancer Res</em></journal_title>
                        <volume>12</volume>
                        <author>   Silva J, Bulman C, McMahon M</author>
                        <first_page>447</first_page>
                        <last_page>463</last_page>
                        <cYear>2015</cYear>
                        <doi></doi>
                        <article_title>BRAFV600E cooperates with PI3'-kinase signaling, independent of AKT, to regulate melanoma cell proliferation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Oncol lett</em></journal_title>
                        <volume>13</volume>
                        <author>   Huang W, Ren C, Huang G, et al.</author>
                        <first_page>921</first_page>
                        <last_page>929</last_page>
                        <cYear>2017</cYear>
                        <doi>10.3892/ol.2016.5469</doi>
                        <article_title>Inhibition of store-operated Ca<sup>2+</sup> entry counteracts the apoptosis of nasopharyngeal carcinoma cells induced by sodium butyrate</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Redox Biol</em></journal_title>
                        <volume>12</volume>
                        <author>   Pant K, Yadav A, Gupta P, et al.</author>
                        <first_page>340</first_page>
                        <last_page>349</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1016/j.redox.2017.03.006</doi>
                        <article_title>Redox Biology Butyrate induces ROS-mediated apoptosis by modulating miR-22/SIRT-1 pathway in hepatic cancer cells</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Phytomedicine</em></journal_title>
                        <volume>35</volume>
                        <author>   Seifrtová M, Havelek R, Cahlíková L, et al.</author>
                        <first_page>1</first_page>
                        <last_page>10</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1016/j.phymed.2017.08.019</doi>
                        <article_title>Haemanthamine alters sodium butyrate-induced histone acetylation, p21 WAF1/Cip1 expression, Chk1 and Chk2 activation and leads to increased growth inhibition and death in A2780 ovarian cancer cells</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Int J Biochem Cell Biol</em></journal_title>
                        <volume>42</volume>
                        <author>   Abramova MV, Zatulovskiy EA, Svetlikova SB, et al.</author>
                        <first_page>1847</first_page>
                        <last_page>1855</last_page>
                        <cYear>2010</cYear>
                        <doi>10.1016/j.biocel.2010.08.001</doi>
                        <article_title>HDAC inhibitor-induced activation of NF-κB prevents apoptotic response of E1A+Ras-transformed cells to proapoptotic stimuli</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Biol Chem</em></journal_title>
                        <volume>281</volume>
                        <author>   Abramova M, Pospelova T, Nikulenkov F, et al.</author>
                        <first_page>21040</first_page>
                        <last_page>21051</last_page>
                        <cYear>2006</cYear>
                        <doi>10.1074/jbc.M511059200</doi>
                        <article_title>G1/S Arrest induced by histone deacetylase inhibitor sodium butyrate in E1A+ Ras-transformed cells is mediated through down-regulation of E2F activity and stabilization of beta-Catenin</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cell Cycle</em></journal_title>
                        <volume>8</volume>
                        <author>   Pospelova TV, Demidenko ZN, Bukreeva EI, et al.</author>
                        <first_page>4112</first_page>
                        <last_page>4118</last_page>
                        <cYear>2009</cYear>
                        <doi>10.4161/cc.8.24.10215</doi>
                        <article_title>Pseudo-DNA damage response in senescent cells</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cancer Ther</em></journal_title>
                        <volume>6</volume>
                        <author>   Oh SY, Sohn YW, Park JW, et al.</author>
                        <first_page>2178</first_page>
                        <last_page>2187</last_page>
                        <cYear>2007</cYear>
                        <doi>10.1158/1535-7163.MCT-07-0111</doi>
                        <article_title>Selective cell death of oncogenic Akt-transduced brain cancer cells by etoposide through reactive oxygen species mediated damage</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>The EMBO J</em></journal_title>
                        <volume>15</volume>
                        <author>   Alessi DR, Andjelkovic M, Caudwell B, et al.</author>
                        <first_page>6541</first_page>
                        <last_page>6551</last_page>
                        <cYear>1996</cYear>
                        <doi></doi>
                        <article_title>Mechanism of activation of protein kinase B by insulin and IGF-1</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Exp Physiol</em></journal_title>
                        <volume>4</volume>
                        <author>   Lu Q, Zhai Y, Cheng Q, et al.</author>
                        <first_page>934</first_page>
                        <last_page>945</last_page>
                        <cYear>2013</cYear>
                        <doi></doi>
                        <article_title>The Akt–FoxO3a–manganese superoxide dismutase pathway is involved in the regulation of oxidative stress in diabetic nephropathy</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Exp Med</em></journal_title>
                        <volume>200</volume>
                        <author>   Fallarino F, Bianchi R, Orabona C, et al.</author>
                        <first_page>1051</first_page>
                        <last_page>1062</last_page>
                        <cYear>2004</cYear>
                        <doi>10.1084/jem.20040942</doi>
                        <article_title>CTLA-4–Ig Activates Forkhead Transcription Factors and Protects Dendritic Cells from Oxidative Stress in Nonobese Diabetic Mice</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em> Int J Nanomed</em></journal_title>
                        <volume>9</volume>
                        <author>   Yang L, Li C, Wan Y, et al.</author>
                        <first_page>4023</first_page>
                        <last_page>4031</last_page>
                        <cYear>2014</cYear>
                        <doi></doi>
                        <article_title>Antioxidative fullerol promotes osteogenesis of Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cell Res</em></journal_title>
                        <volume>21</volume>
                        <author>   Morgan MJ, Liu Z</author>
                        <first_page>103</first_page>
                        <last_page>115</last_page>
                        <cYear>2010</cYear>
                        <doi></doi>
                        <article_title>Crosstalk of reactive oxygen species and NF- κ B signaling</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cancer</em></journal_title>
                        <volume>14</volume>
                        <author>   Nogueira V, Park Y, Chen C, et al.</author>
                        <first_page>458</first_page>
                        <last_page>470</last_page>
                        <cYear>2011</cYear>
                        <doi></doi>
                        <article_title>Akt determines replicative senescence and oxidative or oncogenic premature senescence and sensitizes cells to oxidative apoptosis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cell</em></journal_title>
                        <volume>14</volume>
                        <author>   Sealy L, Chalkley R</author>
                        <first_page>115</first_page>
                        <last_page>121</last_page>
                        <cYear>1978</cYear>
                        <doi>10.1016/0092-8674(78)90306-9</doi>
                        <article_title>The effect of sodium butyrate on histone modification</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cancer</em></journal_title>
                        <volume>16</volume>
                        <author>Zhao Y, Hu X, Liu Y, et al.</author>
                        <first_page>79</first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi>10.1186/s12943-017-0648-1</doi>
                        <article_title>ROS signaling under metabolic stress: cross-talk between AMPK and AKT pathway</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Oncogene</em></journal_title>
                        <volume>24</volume>
                        <author>   Plas DR, Thompson CB</author>
                        <first_page>7435</first_page>
                        <last_page>7442</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1038/sj.onc.1209097</doi>
                        <article_title>Akt-dependent transformation: There is more to growth than just surviving</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cell Cycle</em></journal_title>
                        <volume>9</volume>
                        <author>   Romanov V, Abramova M, Svetlikova S, et al.</author>
                        <first_page>3945</first_page>
                        <last_page>3955</last_page>
                        <cYear>2010</cYear>
                        <doi>10.4161/cc.9.19.13160</doi>
                        <article_title>p21Waf1 is required for cellular senescence but not for cell cycle arrest induced by the HDAC inhibitor sodium butyrate</article_title>
                    </citation>
                                   </citation_list>
                <keywords language="en"></keywords>
				<abstract language="en"><![CDATA[HDAC inhibitors (HDACIs) induce irreversible cell cycle arrest and senescence in mouse embryonic fibroblasts transformed with E1A and c-Ha-Ras oncogenes (E1A+Ras cell line). The aging rate has been associated with the production of high levels of Reactive Oxygen Species (ROS). Specific increases of ROS level have been demonstrated as potentially critical for induction and maintenance of cell senescence process. It’s known that HDACs regulate the ROS-dependent FoxO factors, which are responsible for cell growth, proliferation, and longevity. The characteristic ROS increase during aging may be responsible for the decreased HDAC activity, which facilitates the senescent-like phenotype. The objective of this study was to investigate the impact of FoxO transcription factors on HDACIs-induced senescence of E1A+Ras oncogenes transformed cells. This study shows the specific time-dependent effect of HDACI sodium butyrate treatment on FoxO proteins in E1A+Ras cells. Indeed, short-term treatment with NaB results in FoxO activation, which takes place through nuclear translocation, and accompanied by accumulation of such ROS scavengers as MnSOD and SOD2. However, prolonged treatment leads to extensive FoxO degradation and increased intracellular levels of ROS. This degradation is connected with NaB-induced activation of Akt kinase. All of these findings establish that one of the possible mechanism involved in NaB-induced senescence of transformed cells is mediated through down-regulation of FoxO transcription factors and ROS accumulation.]]></abstract>
			</journal_article>
                        <journal_article>
	            	            <titles>
                    <title><![CDATA[Identification of a novel functional miR-143-5p recognition element in the Cystic Fibrosis Transmembrane Conductance Regulator 3’UTR]]></title>
                </titles>
	                            <contributors>
                                </contributors>
                <publication_date media_type="print">
                    <month></month>
                    <year>2018</year>
                </publication_date>
                
                <pages>
                    <first_page>53</first_page>
                    <last_page>62</last_page>
                </pages>
                <doi_data>
                    <doi>10.3934/genet.2018.1.53</doi>
                    <timestamp>2017-09-01 12:00:00.0</timestamp>
					<resource>http://www.aimsciences.org/article/id/1833</resource>
                </doi_data>
	                
                <citation_list>
               	                    <citation key="">
                        <journal_title><em>Science</em></journal_title>
                        <volume>294</volume>
                        <author>         Lee RC, Ambros V</author>
                        <first_page>862</first_page>
                        <last_page>864</last_page>
                        <cYear>1993</cYear>
                        <doi></doi>
                        <article_title>An extensive class of small RNAs in Caenorhabditis elegans</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Life Sci</em></journal_title>
                        <volume>185</volume>
                        <author>         Chandra S, Vimal D, Sharma D, et al.</author>
                        <first_page>8</first_page>
                        <last_page>14</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1016/j.lfs.2017.07.017</doi>
                        <article_title>Role of miRNAs in development and disease: Lessons learnt from small organisms</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cancer Discov</em></journal_title>
                        <volume>6</volume>
                        <author>         Rupaimoole R, Calin GA, Lopez-Berestein G, et al.</author>
                        <first_page>235</first_page>
                        <last_page>246</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1158/2159-8290.CD-15-0893</doi>
                        <article_title>miRNA Deregulation in Cancer Cells and the Tumor Microenvironment</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Biomed Res Int</em></journal_title>
                        <volume>2016</volume>
                        <author>Szymczak I, Wieczfinska J, Pawliczak R</author>
                        <first_page>7802521</first_page>
                        <last_page></last_page>
                        <cYear>2016</cYear>
                        <doi></doi>
                        <article_title>Molecular Background of miRNA Role in Asthma and COPD: An Updated Insight</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Autoimmun Rev</em></journal_title>
                        <volume>15</volume>
                        <author>         Chen JQ, Papp G, Szodoray P</author>
                        <first_page>1171</first_page>
                        <last_page>1180</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1016/j.autrev.2016.09.003</doi>
                        <article_title>The role of microRNAs in the pathogenesis of autoimmune diseases</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Genes Dev</em></journal_title>
                        <volume>18</volume>
                        <author>         Kiriakidou M, Nelson PT, Kouranov A, et al.</author>
                        <first_page>1165</first_page>
                        <last_page>1178</last_page>
                        <cYear>2004</cYear>
                        <doi>10.1101/gad.1184704</doi>
                        <article_title>A combined computational-experimental approach predicts human microRNA targets</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Genes Dev</em></journal_title>
                        <volume>19</volume>
                        <author>         Lai EC, Tam B, Rubin GM</author>
                        <first_page>1067</first_page>
                        <last_page>1080</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1101/gad.1291905</doi>
                        <article_title>Pervasive regulation of Drosophila Notch target genes by GY-box-, Brd-box-, and K-box-class microRNAs</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Adv Exp Med Biol</em></journal_title>
                        <volume>937</volume>
                        <author>         Vodicka P, Pardini B, Vymetalkova V, et al.</author>
                        <first_page>123</first_page>
                        <last_page>149</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1007/978-3-319-42059-2_7</doi>
                        <article_title>Polymorphisms in Non-coding RNA Genes and Their Targets Sites as Risk Factors of Sporadic Colorectal Cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Am J Hypertens</em></journal_title>
                        <volume>24</volume>
                        <author>         Nossent AY, Hansen JL, Doggen C, et al.</author>
                        <first_page>999</first_page>
                        <last_page>1006</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1038/ajh.2011.92</doi>
                        <article_title>SNPs in microRNA binding sites in 3'-UTRs of RAAS genes influence arterial blood pressure and risk of myocardial infarction</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Int J Genomics</em></journal_title>
                        <volume>2017</volume>
                        <author>Wang X, Jiang H, Wu W, et al.</author>
                        <first_page>1674827</first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi></doi>
                        <article_title>An Integrating Approach for Genome-Wide Screening of MicroRNA Polymorphisms Mediated Drug Response Alterations</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nucleic Acids Res</em></journal_title>
                        <volume>39</volume>
                        <author>     Thomson DW, Bracken CP, Goodall GJ</author>
                        <first_page>6845</first_page>
                        <last_page>6853</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1093/nar/gkr330</doi>
                        <article_title>Experimental strategies for microRNA target identification</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Biochem J</em></journal_title>
                        <volume>438</volume>
                        <author>     Gillen AE, Gosalia N, Leir SH, et al.</author>
                        <first_page>25</first_page>
                        <last_page>32</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1042/BJ20110672</doi>
                        <article_title>MicroRNA regulation of expression of the cystic fibrosis transmembrane conductance regulator gene</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS One</em></journal_title>
                        <volume>6</volume>
                        <author>Megiorni F, Cialfi S, Dominici C, et al.</author>
                        <first_page>e26601</first_page>
                        <last_page></last_page>
                        <cYear>2011</cYear>
                        <doi>10.1371/journal.pone.0026601</doi>
                        <article_title>Synergistic post-transcriptional regulation of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) by miR-101 and miR-494 specific binding</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS One</em></journal_title>
                        <volume>7</volume>
                        <author>Hassan F, Nuovo GJ, Crawford M, et al.</author>
                        <first_page>e50837</first_page>
                        <last_page></last_page>
                        <cYear>2012</cYear>
                        <doi>10.1371/journal.pone.0050837</doi>
                        <article_title>MiR-101 and miR-144 regulate the expression of the CFTR chloride channel in the lung</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Immunol</em></journal_title>
                        <volume>190</volume>
                        <author>     Oglesby IK, Chotirmall SH, McElvaney NG, et al.</author>
                        <first_page>3354</first_page>
                        <last_page>3362</last_page>
                        <cYear>2013</cYear>
                        <doi>10.4049/jimmunol.1202960</doi>
                        <article_title>Regulation of cystic fibrosis transmembrane conductance regulator by microRNA-145, -223, and -494 is altered in ΔF508 cystic fibrosis airway epithelium</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS One</em></journal_title>
                        <volume>8</volume>
                        <author>Amato F, Seia M, Giordano S, et al.</author>
                        <first_page>e60448</first_page>
                        <last_page></last_page>
                        <cYear>2013</cYear>
                        <doi>10.1371/journal.pone.0060448</doi>
                        <article_title>Gene mutation in microRNA target sites of CFTR gene: a novel pathogenetic mechanism in cystic fibrosis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Am J Respir Cell Mol Biol</em></journal_title>
                        <volume>49</volume>
                        <author>     Ramachandran S, Karp PH, Osterhaus SR, et al.</author>
                        <first_page>544</first_page>
                        <last_page>551</last_page>
                        <cYear>2013</cYear>
                        <doi>10.1165/rcmb.2012-0430OC</doi>
                        <article_title>Post-transcriptional regulation of cystic fibrosis transmembrane conductance regulator expression and function by microRNAs</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Eur Respir J</em></journal_title>
                        <volume>45</volume>
                        <author>     Viart V, Bergougnoux A, Bonini J, et al.</author>
                        <first_page>116</first_page>
                        <last_page>128</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1183/09031936.00113214</doi>
                        <article_title>Transcription factors and miRNAs that regulate fetal to adult CFTR expression change are new targets for cystic fibrosis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title></journal_title>
                        <volume></volume>
                        <author></author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear></cYear>
                        <doi></doi>
                        <article_title>World Health Organization. The molecular genetic epidemiology of cystic</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title></journal_title>
                        <volume></volume>
                        <author></author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear></cYear>
                        <doi></doi>
                        <article_title>The Clinical and Functional TRanslation of CFTR (CFTR2), 2017. Available</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mediators Inflamm</em></journal_title>
                        <volume>2015</volume>
                        <author>McKiernan PJ, Greene CM</author>
                        <first_page>529642</first_page>
                        <last_page></last_page>
                        <cYear>2015</cYear>
                        <doi></doi>
                        <article_title>MicroRNA Dysregulation in Cystic Fibrosis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Immunol</em></journal_title>
                        <volume>184</volume>
                        <author>     Oglesby IK, Bray IM, Chotirmall SH, et al.</author>
                        <first_page>1702</first_page>
                        <last_page>1709</last_page>
                        <cYear>2010</cYear>
                        <doi>10.4049/jimmunol.0902669</doi>
                        <article_title>miR-126 is downregulated in cystic fibrosis airway epithelial cells and regulates TOM1 expression</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Eur Respir J</em></journal_title>
                        <volume>46</volume>
                        <author>     Oglesby IK, Vencken SF, Agrawal R, et al.</author>
                        <first_page>1350</first_page>
                        <last_page>1360</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1183/09031936.00163414</doi>
                        <article_title>miR-17 overexpression in cystic fibrosis airway epithelial cells decreases interleukin-8 production</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Am J Respir Crit Care Med</em></journal_title>
                        <volume>192</volume>
                        <author>     Mirković B, Murray MA, Lavelle GM, et al.</author>
                        <first_page>1314</first_page>
                        <last_page>1324</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1164/rccm.201505-0943OC</doi>
                        <article_title>The Role of Short-Chain Fatty Acids, Produced by Anaerobic Bacteria, in the Cystic Fibrosis Airway</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Methods</em></journal_title>
                        <volume>25</volume>
                        <author>     Livak KJ, Schmittgen TD</author>
                        <first_page>402</first_page>
                        <last_page>408</last_page>
                        <cYear>2001</cYear>
                        <doi>10.1006/meth.2001.1262</doi>
                        <article_title>Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Elife</em> 4.</journal_title>
                        <volume></volume>
                        <author>Agarwal V, Bell GW, Nam JW, et al.</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2015</cYear>
                        <doi></doi>
                        <article_title>Predicting effective microRNA target sites in mammalian mRNAs</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Genome Biol</em></journal_title>
                        <volume>11</volume>
                        <author>Betel D, Koppal A, Agius P, et al.</author>
                        <first_page>R90</first_page>
                        <last_page></last_page>
                        <cYear>2010</cYear>
                        <doi>10.1186/gb-2010-11-8-r90</doi>
                        <article_title>Comprehensive modeling of microRNA targets predicts functional non-conserved and non-canonical sites</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cell</em></journal_title>
                        <volume>126</volume>
                        <author>     Miranda KC, Huynh T, Tay Y, et al.</author>
                        <first_page>1203</first_page>
                        <last_page>1217</last_page>
                        <cYear>2006</cYear>
                        <doi>10.1016/j.cell.2006.07.031</doi>
                        <article_title>A pattern-based method for the identification of MicroRNA binding sites and their corresponding heteroduplexes</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Methods Mol Biol</em></journal_title>
                        <volume>1296</volume>
                        <author>     Clément T, Salone V, Rederstorff M</author>
                        <first_page>187</first_page>
                        <last_page>198</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1007/978-1-4939-2547-6_17</doi>
                        <article_title>Dual luciferase gene reporter assays to study miRNA function</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Biomed Pharmacother</em></journal_title>
                        <volume>91</volume>
                        <author>     Karimi L, Mansoori B, Shanebandi D, et al.</author>
                        <first_page>121</first_page>
                        <last_page>131</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1016/j.biopha.2017.04.060</doi>
                        <article_title>Function of microRNA-143 in different signal pathways in cancer: New insights into cancer therapy</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cell Physiol Biochem</em></journal_title>
                        <volume>42</volume>
                        <author>     He M, Zhan M, Chen W, et al.</author>
                        <first_page>2078</first_page>
                        <last_page>2092</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1159/000479903</doi>
                        <article_title>MiR-143-5p Deficiency Triggers EMT and Metastasis by Targeting HIF-1α in Gallbladder Cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>World J Gastroenterol</em></journal_title>
                        <volume>19</volume>
                        <author>     Wu XL, Cheng B, Li PY, et al.</author>
                        <first_page>7758</first_page>
                        <last_page>7765</last_page>
                        <cYear>2013</cYear>
                        <doi>10.3748/wjg.v19.i43.7758</doi>
                        <article_title>MicroRNA-143 suppresses gastric cancer cell growth and induces apoptosis by targeting COX-2</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title></journal_title>
                        <volume></volume>
                        <author></author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear></cYear>
                        <doi></doi>
                        <article_title>FDA expands approved use of Kalydeco to treat additional mutations of cystic fibrosis, FDA News Release, 2017. Available</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Pharmacol Ther</em></journal_title>
                        <volume>170</volume>
                        <author>     Fajac I, De Boeck K</author>
                        <first_page>205</first_page>
                        <last_page>211</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1016/j.pharmthera.2016.11.009</doi>
                        <article_title>New horizons for cystic fibrosis treatment</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Thorax</em></journal_title>
                        <volume>67</volume>
                        <author>     Clancy JP, Rowe SM, Accurso FJ, et al.</author>
                        <first_page>12</first_page>
                        <last_page>18</last_page>
                        <cYear>2012</cYear>
                        <doi>10.1136/thoraxjnl-2011-200393</doi>
                        <article_title>Results of a phase IIa study of VX-809, an investigational CFTR corrector compound, in subjects with cystic fibrosis homozygous for the F508del-CFTR mutation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Lancet Respir Med</em></journal_title>
                        <volume>2</volume>
                        <author>     Boyle MP, Bell SC, Konstan MW, et al.</author>
                        <first_page>527</first_page>
                        <last_page>538</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1016/S2213-2600(14)70132-8</doi>
                        <article_title>A CFTR corrector (lumacaftor) and a CFTR potentiator (ivacaftor) for treatment of patients with cystic fibrosis who have a phe508del CFTR mutation: a phase 2 randomised controlled trial</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>N Engl J Med</em></journal_title>
                        <volume>373</volume>
                        <author>     Wainwright CE, Elborn JS, Ramsey BW, et al.</author>
                        <first_page>220</first_page>
                        <last_page>231</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1056/NEJMoa1409547</doi>
                        <article_title>Lumacaftor-Ivacaftor in Patients with Cystic Fibrosis Homozygous for Phe508del CFTR</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Molecules</em></journal_title>
                        <volume>22</volume>
                        <author>Zarrilli F, Amato F, Morgillo CM, et al.</author>
                        <first_page>1144</first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi>10.3390/molecules22071144</doi>
                        <article_title>Peptide Nucleic Acids as miRNA Target Protectors for the Treatment of Cystic Fibrosis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>Implications in Cystic Fibrosis and Chronic Obstructive Pulmonary Disease. <em>Ann Am Thorac Soc</em> 13 Suppl</journal_title>
                        <volume>2</volume>
                        <author>     Cantin AM</author>
                        <first_page>S150</first_page>
                        <last_page>S155</last_page>
                        <cYear>2016</cYear>
                        <doi></doi>
                        <article_title>Cystic Fibrosis Transmembrane Conductance Regulator</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Ann Am Thorac Soc</em> 13 Suppl</journal_title>
                        <volume>2</volume>
                        <author>     Solomon GM, Raju SV, Dransfield MT, et al.</author>
                        <first_page>S169</first_page>
                        <last_page>S176</last_page>
                        <cYear>2016</cYear>
                        <doi></doi>
                        <article_title>Therapeutic Approaches to Acquired Cystic Fibrosis Transmembrane Conductance Regulator Dysfunction in Chronic Bronchitis</article_title>
                    </citation>
                                   </citation_list>
                <keywords language="en"></keywords>
				<abstract language="en"><![CDATA[MicroRNAs (miRNAs) are small non-coding RNAs involved in regulation of gene expression. They bind in a sequence-specific manner to miRNA recognition elements (MREs) located in the 3’ untranslated region (UTR) of target mRNAs and prevent mRNA translation. MiRNA expression is dysregulated in cystic fibrosis (CF), affecting several biological processes including ion conductance in the epithelial cells of the lung. We previously reported that miR-143 is up-regulated in CF bronchial brushings compared to non-CF. Here we identified two predicted binding sites for miR-143-5p (starting at residues 558 and 644) on the <em>CFTR</em> mRNA, and aimed to assess whether <em>CFTR</em> is a true molecular target of miR-143-5p. Expression of miR-143-5p was found to be up-regulated in a panel of CF vs non-CF cell lines (1.7-fold, P = 0.0165), and its levels were increased <em>in vitro</em> after 20 hours treatment with bronchoalveolar lavage fluid from CF patients compared to vehicle-treated cells (3.3-fold, P = 0.0319). Luciferase assays were performed to elucidate direct miRNA::target interactions and showed that miR-143-5p significantly decreased the reporter activity when carrying the wild-type full length sequence of <em>CFTR</em> 3’UTR (minus 15%, P = 0.005). This repression was rescued by the disruption of the first, but not the second, predicted MRE, suggesting that the residue starting at 558 was the actual active binding site. In conclusion, we here showed that miR-143-5p modestly but significantly inhibits CFTR, improving the knowledge on functional MREs within the <em>CFTR</em> 3’UTR. This could lead to the development of novel therapeutic strategies where miRNA-mediated CFTR repression is blocked thereby possibly increasing the efficacy of the currently available CFTR modulators.]]></abstract>
			</journal_article>
                        <journal_article>
	            	            <titles>
                    <title><![CDATA[Rpb5, a subunit shared by eukaryotic RNA polymerases, cooperates with prefoldin-like Bud27/URI]]></title>
                </titles>
	                            <contributors>
                                </contributors>
                <publication_date media_type="print">
                    <month></month>
                    <year>2018</year>
                </publication_date>
                
                <pages>
                    <first_page>63</first_page>
                    <last_page>74</last_page>
                </pages>
                <doi_data>
                    <doi>10.3934/genet.2018.1.63</doi>
                    <timestamp>2017-10-08 12:00:00.0</timestamp>
					<resource>http://www.aimsciences.org/article/id/1840</resource>
                </doi_data>
	                
                <citation_list>
               	                    <citation key="">
                        <journal_title><em>Nat Commun</em></journal_title>
                        <volume>5</volume>
                        <author>Jun SH, Hirata A, Kanai T, et al.</author>
                        <first_page>5132</first_page>
                        <last_page></last_page>
                        <cYear>2014</cYear>
                        <doi>10.1038/ncomms6132</doi>
                        <article_title>The X-ray crystal structure of the euryarchaeal RNA polymerase in an open-clamp configuration</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Open Biol</em></journal_title>
                        <volume>7</volume>
                        <author>Leśniewska E, Boguta M</author>
                        <first_page>170001</first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi>10.1098/rsob.170001</doi>
                        <article_title>Novel layers of RNA polymerase III control affecting tRNA gene transcription in eukaryotes</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Elife</em>, 6.</journal_title>
                        <volume></volume>
                        <author>Torreira E, Louro JA, Pazos I, et al.</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi></doi>
                        <article_title>The dynamic assembly of distinct RNA polymerase I complexes modulates rDNA transcription</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nat Rev Microbiol</em></journal_title>
                        <volume>9</volume>
                        <author>         Werner F, Grohmann D</author>
                        <first_page>85</first_page>
                        <last_page>98</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1038/nrmicro2507</doi>
                        <article_title>Evolution of multisubunit RNA polymerases in the three domains of life</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Curr Opin Struct Biol</em></journal_title>
                        <volume>19</volume>
                        <author>         Werner M, Thuriaux P, Soutourina J</author>
                        <first_page>740</first_page>
                        <last_page>745</last_page>
                        <cYear>2009</cYear>
                        <doi>10.1016/j.sbi.2009.10.005</doi>
                        <article_title>Structure-function analysis of RNA polymerases I and III</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Curr Opin Struct Biol</em></journal_title>
                        <volume>47</volume>
                        <author>         Khatter H, Vorländer MK, Müller CW</author>
                        <first_page>88</first_page>
                        <last_page>94</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1016/j.sbi.2017.05.008</doi>
                        <article_title>RNA polymerase I and III: Similar yet unique</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell</em></journal_title>
                        <volume>66</volume>
                        <author>Fong N, Saldi T, Sheridan RM, et al.</author>
                        <first_page>546</first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi>10.1016/j.molcel.2017.04.016</doi>
                        <article_title>RNA Pol II Dynamics Modulate Co-transcriptional Chromatin Modification, CTD Phosphorylation, and Transcriptional Direction</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nat Rev Mol Cell Biol1</em></journal_title>
                        <volume>12</volume>
                        <author>         Haag JR, Pikaard CS</author>
                        <first_page>483</first_page>
                        <last_page>492</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1038/nrm3152</doi>
                        <article_title>Multisubunit RNA polymerases IV and V: Purveyors of non-coding RNA for plant gene silencing</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Plant J Cell Mol Biol</em></journal_title>
                        <volume>79</volume>
                        <author>         Böhmdorfer G, Rowley MJ, Kuciński J, et al.</author>
                        <first_page>181</first_page>
                        <last_page>191</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1111/tpj.12563</doi>
                        <article_title>RNA‐directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cell Rep</em></journal_title>
                        <volume>9</volume>
                        <author>     Haag JR, Brower-Toland B, Krieger EK, et al.</author>
                        <first_page>378</first_page>
                        <last_page>390</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1016/j.celrep.2014.08.067</doi>
                        <article_title>Functional diversification of maize RNA polymerase IV and V subtypes via alternative catalytic subunits</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nature</em></journal_title>
                        <volume>529</volume>
                        <author>     Bernecky C, Herzog F, Baumeister W, et al.</author>
                        <first_page>551</first_page>
                        <last_page>554</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1038/nature16482</doi>
                        <article_title>Structure of transcribing mammalian RNA polymerase II</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Trends Genet</em></journal_title>
                        <volume>24</volume>
                        <author>     Kwapisz M, Beckouët F, Thuriaux P</author>
                        <first_page>211</first_page>
                        <last_page>215</last_page>
                        <cYear>2008</cYear>
                        <doi>10.1016/j.tig.2008.02.002</doi>
                        <article_title>Early evolution of eukaryotic DNA-dependent RNA polymerases</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nucleic Acids Res</em></journal_title>
                        <volume>35</volume>
                        <author>     Zaros C, Briand JF, Boulard Y, et al.</author>
                        <first_page>634</first_page>
                        <last_page>647</last_page>
                        <cYear>2007</cYear>
                        <doi>10.1093/nar/gkl686</doi>
                        <article_title>Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Genes Dev</em></journal_title>
                        <volume>4</volume>
                        <author>     Woychik NA, Liao SM, Kolodziej PA, et al.</author>
                        <first_page>313</first_page>
                        <last_page>323</last_page>
                        <cYear>1990</cYear>
                        <doi>10.1101/gad.4.3.313</doi>
                        <article_title>Subunits shared by eukaryotic nuclear RNA polymerases</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell Biol</em></journal_title>
                        <volume>15</volume>
                        <author>     Shpakovski GV, Acker J, Wintzerith M, et al.</author>
                        <first_page>4702</first_page>
                        <last_page>4710</last_page>
                        <cYear>1995</cYear>
                        <doi>10.1128/MCB.15.9.4702</doi>
                        <article_title>Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Biochem Soc Trans</em></journal_title>
                        <volume>33</volume>
                        <author>     Kelly S, Wickstead B, Gull K</author>
                        <first_page>1435</first_page>
                        <last_page>1437</last_page>
                        <cYear>2005</cYear>
                        <doi></doi>
                        <article_title>An in silico analysis of trypanosomatid RNA polymerases: Insights into their unusual transcription</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Biol Chem</em></journal_title>
                        <volume>280</volume>
                        <author>     Armache KJ, Mitterweger S, Meinhart A, et al.</author>
                        <first_page>7131</first_page>
                        <last_page>7134</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1074/jbc.M413038200</doi>
                        <article_title>Structures of complete RNA polymerase II and its subcomplex, Rpb4/7</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>1-A resolution: Implications for the initiation of transcription. <em>Proc Natl Acad Sci U S A</em></journal_title>
                        <volume>100</volume>
                        <author>     Bushnell DA, Kornberg RD</author>
                        <first_page>6969</first_page>
                        <last_page>6973</last_page>
                        <cYear>2003</cYear>
                        <doi></doi>
                        <article_title>Complete, 12-subunit RNA polymerase II at 4</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>8 Ångstrom resolution. <em>Science</em></journal_title>
                        <volume>292</volume>
                        <author>     Cramer P, Bushnell DA, Kornberg RD</author>
                        <first_page>1863</first_page>
                        <last_page>1876</last_page>
                        <cYear>2001</cYear>
                        <doi></doi>
                        <article_title>Structural basis of transcription: RNA polymerase II at 2</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nature</em></journal_title>
                        <volume>502</volume>
                        <author>     Fernándeztornero C, Morenomorcillo M, Rashid UJ, et al.</author>
                        <first_page>644</first_page>
                        <last_page>649</last_page>
                        <cYear>2013</cYear>
                        <doi>10.1038/nature12636</doi>
                        <article_title>Crystal structure of the  14-subunit RNA polymerase I</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nature</em></journal_title>
                        <volume>528</volume>
                        <author>     Hoffmann NA, Jakobi AJ, Maria MM, et al.</author>
                        <first_page>231</first_page>
                        <last_page>236</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1038/nature16143</doi>
                        <article_title>Molecular structures of unbound and transcribing RNA polymerase III</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell Biol</em></journal_title>
                        <volume>7</volume>
                        <author>     Nonet M, Scafe C, Sexton J, et al.</author>
                        <first_page>1602</first_page>
                        <last_page>1611</last_page>
                        <cYear>1987</cYear>
                        <doi>10.1128/MCB.7.5.1602</doi>
                        <article_title>Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Curr Genet</em></journal_title>
                        <volume>44</volume>
                        <author>     Mitsuzawa H, Ishihama A</author>
                        <first_page>287</first_page>
                        <last_page>294</last_page>
                        <cYear>2004</cYear>
                        <doi>10.1007/s00294-003-0446-8</doi>
                        <article_title>RNA polymerase II transcription apparatus in Schizosaccharomyces pombe</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Proc Natl Acad Sci U S A</em></journal_title>
                        <volume>92</volume>
                        <author>     Langer D, Hain J, Thuriaux P, et al.</author>
                        <first_page>5768</first_page>
                        <last_page>5772</last_page>
                        <cYear>1995</cYear>
                        <doi>10.1073/pnas.92.13.5768</doi>
                        <article_title>Transcription in archaea: Similarity to that in eucarya</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS Biol</em></journal_title>
                        <volume>7</volume>
                        <author>Korkhin Y, Unligil UM, Littlefield O, et al.</author>
                        <first_page>e1000102</first_page>
                        <last_page></last_page>
                        <cYear>2009</cYear>
                        <doi>10.1371/journal.pbio.1000102</doi>
                        <article_title>Evolution of complex RNA polymerases: The complete archaeal RNA polymerase structure</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS One</em></journal_title>
                        <volume>9</volume>
                        <author>Sommer B, Waege I, Pöllmann D, et al.</author>
                        <first_page>e87485</first_page>
                        <last_page></last_page>
                        <cYear>2014</cYear>
                        <doi>10.1371/journal.pone.0087485</doi>
                        <article_title>Activation of a chimeric Rpb5/RpoH subunit using library selection</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Virus Res</em></journal_title>
                        <volume>117</volume>
                        <author>     Iyer LM, Balaji S, Koonin EV, et al.</author>
                        <first_page>156</first_page>
                        <last_page>184</last_page>
                        <cYear>2006</cYear>
                        <doi>10.1016/j.virusres.2006.01.009</doi>
                        <article_title>Evolutionary genomics of nucleo-cytoplasmic large DNA viruses</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>2-megabase genome sequence of Mimivirus. <em>Science</em></journal_title>
                        <volume>306</volume>
                        <author>     Raoult D, Audic S, Robert C, et al.</author>
                        <first_page>1344</first_page>
                        <last_page>1350</last_page>
                        <cYear>2004</cYear>
                        <doi></doi>
                        <article_title>The 1</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Science</em></journal_title>
                        <volume>288</volume>
                        <author>     Cramer P, Bushnell DA, Fu J, et al.</author>
                        <first_page>640</first_page>
                        <last_page>649</last_page>
                        <cYear>2000</cYear>
                        <doi>10.1126/science.288.5466.640</doi>
                        <article_title>Architecture of RNA polymerase II and implications for the transcription mechanism</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Proc Natl Acad Sci U S A</em></journal_title>
                        <volume>97</volume>
                        <author>     Todone F, Brick P, Onesti S</author>
                        <first_page>6306</first_page>
                        <last_page>6310</last_page>
                        <cYear>2000</cYear>
                        <doi>10.1073/pnas.97.12.6306</doi>
                        <article_title>Crystal structure of RPB5, a universal eukaryotic RNA polymerase subunit and transcription factor interaction target</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>RNA</em></journal_title>
                        <volume>6</volume>
                        <author>     Navarro F, Thuriaux P</author>
                        <first_page>103</first_page>
                        <last_page>110</last_page>
                        <cYear>2000</cYear>
                        <doi>10.1017/S1355838200991714</doi>
                        <article_title>In vivo misreading by tRNA overdose</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Proc Natl Acad Sci U S A</em></journal_title>
                        <volume>96</volume>
                        <author>     Flores A, Briand JF, Gadal O, et al.</author>
                        <first_page>7815</first_page>
                        <last_page>7820</last_page>
                        <cYear>1999</cYear>
                        <doi>10.1073/pnas.96.14.7815</doi>
                        <article_title>A protein-protein interaction map of yeast RNA polymerase III</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nucleic Acids Res</em></journal_title>
                        <volume>38</volume>
                        <author>     Grünberg S, Reich C, Zeller ME, et al.</author>
                        <first_page>1950</first_page>
                        <last_page>1963</last_page>
                        <cYear>2010</cYear>
                        <doi>10.1093/nar/gkp1190</doi>
                        <article_title>Rearrangement of the RNA polymerase subunit H and the lower jaw in archaeal elongation complexes</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell</em></journal_title>
                        <volume>59</volume>
                        <author>     Barnes CO, Calero M, Malik I, et al.</author>
                        <first_page>258</first_page>
                        <last_page>269</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1016/j.molcel.2015.06.034</doi>
                        <article_title>Crystal structure of a transcribing RNA polymerase II complex reveals a complete transcription bubble</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Biol Chem</em></journal_title>
                        <volume>272</volume>
                        <author>     Kimura M, Ishiguro A, Ishihama A</author>
                        <first_page>25851</first_page>
                        <last_page>25855</last_page>
                        <cYear>1997</cYear>
                        <doi>10.1074/jbc.272.41.25851</doi>
                        <article_title>RNA polymerase II subunits 2, 3, and 11 form a core subassembly with DNA binding activity</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell Biol</em></journal_title>
                        <volume>13</volume>
                        <author>     Bartholomew B, Durkovich D, Kassavetis G, et al.</author>
                        <first_page>942</first_page>
                        <last_page>952</last_page>
                        <cYear>1993</cYear>
                        <doi>10.1128/MCB.13.2.942</doi>
                        <article_title>Orientation and topography of RNA polymerase III in transcription complexes</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nature</em></journal_title>
                        <volume>495</volume>
                        <author>He Y, Fang J, Taatjes DJ, et al.</author>
                        <first_page>481</first_page>
                        <last_page></last_page>
                        <cYear>2013</cYear>
                        <doi>10.1038/nature11991</doi>
                        <article_title>Structural visualization of key steps in human transcription initiation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Proc Natl Acad Sci U S A</em></journal_title>
                        <volume>112</volume>
                        <author>     Fishburn J, Tomko E, Galburt E, et al.</author>
                        <first_page>3961</first_page>
                        <last_page>3966</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1073/pnas.1417709112</doi>
                        <article_title>Double-stranded DNA translocase activity of transcription factor TFIIH and the mechanism of RNA polymerase II open complex formation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell Biol</em></journal_title>
                        <volume>26</volume>
                        <author>     Soutourina J, Bordas-Le FV, Gendrel G, et al.</author>
                        <first_page>4920</first_page>
                        <last_page>4933</last_page>
                        <cYear>2006</cYear>
                        <doi>10.1128/MCB.00415-06</doi>
                        <article_title>Rsc4 connects the chromatin remodeler RSC to RNA polymerases</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nat Rev Microbiol</em></journal_title>
                        <volume>13</volume>
                        <author>Peeters E, Driessen RP, Werner F, et al.</author>
                        <first_page>333</first_page>
                        <last_page></last_page>
                        <cYear>2015</cYear>
                        <doi>10.1038/nrmicro3467</doi>
                        <article_title>The interplay between nucleoid organization and transcription in archaeal genomes</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Proc Natl Acad Sci U S A</em></journal_title>
                        <volume>95</volume>
                        <author>     Miyao T, Woychik NA</author>
                        <first_page>15281</first_page>
                        <last_page>15286</last_page>
                        <cYear>1998</cYear>
                        <doi>10.1073/pnas.95.26.15281</doi>
                        <article_title>RNA polymerase subunit RPB5 plays a role in transcriptional activation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>EMBO J</em></journal_title>
                        <volume>14</volume>
                        <author>     Cheong JH, Yi M, Lin Y, et al.</author>
                        <first_page>143</first_page>
                        <last_page>150</last_page>
                        <cYear>1995</cYear>
                        <doi></doi>
                        <article_title>Human RPB5, a subunit shared by eukaryotic nuclear RNA polymerases, binds human hepatitis B virus X protein and may play a role in X transactivation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em> Mol Cell Biol</em></journal_title>
                        <volume>18</volume>
                        <author>     Haviv I, Shamay MG, Shaul Y</author>
                        <first_page>1562</first_page>
                        <last_page>1569</last_page>
                        <cYear>1998</cYear>
                        <doi>10.1128/MCB.18.3.1562</doi>
                        <article_title>Hepatitis B virus pX targets TFIIB in transcription coactivation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell Biol</em></journal_title>
                        <volume>18</volume>
                        <author>     Dorjsuren D, Lin Y, Wei W, et al.</author>
                        <first_page>7546</first_page>
                        <last_page>7555</last_page>
                        <cYear>1998</cYear>
                        <doi>10.1128/MCB.18.12.7546</doi>
                        <article_title>RMP, a novel RNA polymerase II subunit 5-interacting protein, counteracts transactivation by hepatitis B virus X protein</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Biochem</em></journal_title>
                        <volume>138</volume>
                        <author>     Le TT, Zhang S, Hayashi N, et al.</author>
                        <first_page>215</first_page>
                        <last_page>224</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1093/jb/mvi119</doi>
                        <article_title>Mutational analysis of human RNA polymerase II subunit 5 (RPB5): The residues critical for interactions with TFIIF subunit RAP30 and hepatitis B virus X protein</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Biol Chem</em></journal_title>
                        <volume>276</volume>
                        <author>     Wei W, Dorjsuren D, Lin Y, et al.</author>
                        <first_page>12266</first_page>
                        <last_page>12273</last_page>
                        <cYear>2001</cYear>
                        <doi>10.1074/jbc.M009634200</doi>
                        <article_title>Direct interaction between the subunit RAP30 of transcription factor IIF (TFIIF) and RNA polymerase subunit 5, which contributes to the association between TFIIF and RNA polymerase II</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cell Res</em></journal_title>
                        <volume>13</volume>
                        <author>Wei W, Gu JX, Zhu CQ, et al.</author>
                        <first_page>111</first_page>
                        <last_page></last_page>
                        <cYear>2003</cYear>
                        <doi>10.1038/sj.cr.7290155</doi>
                        <article_title>Interaction with general transcription factor IIF (TFIIF)  is required for the suppression of activated transcription by RPB5-mediating protein (RMP)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Genomics</em></journal_title>
                        <volume>38</volume>
                        <author>     Morohoshi F, Arai K, Takahashi EI, et al.</author>
                        <first_page>51</first_page>
                        <last_page>57</last_page>
                        <cYear>1996</cYear>
                        <doi>10.1006/geno.1996.0591</doi>
                        <article_title>Cloning and Mapping of a Human RBP56 Gene Encoding a Putative RNA Binding Protein Similar to FUS/TLS and EWS Proteins</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>EMBO J</em></journal_title>
                        <volume>15</volume>
                        <author>Bertolotti A, Lutz Y, Heard DJ, et al.</author>
                        <first_page>5022</first_page>
                        <last_page></last_page>
                        <cYear>1996</cYear>
                        <doi></doi>
                        <article_title>HTAF(II)68, a novel RNA/ssDNA-binding protein with homology to the pro-oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell Biol</em></journal_title>
                        <volume>18</volume>
                        <author>     Bertolotti A, Melot T, Acker J, et al.</author>
                        <first_page>1489</first_page>
                        <last_page>1497</last_page>
                        <cYear>1998</cYear>
                        <doi>10.1128/MCB.18.3.1489</doi>
                        <article_title>EWS, but not EWS-FLI-1, is associated with both TFIID and RNA polymerase II: Interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell Biol</em></journal_title>
                        <volume>19</volume>
                        <author>     Makino Y, Yogosawa S, Kayukawa K, et al.</author>
                        <first_page>7951</first_page>
                        <last_page>7960</last_page>
                        <cYear>1999</cYear>
                        <doi>10.1128/MCB.19.12.7951</doi>
                        <article_title>TATA-binding protein-interacting protein 120, TIP120, stimulates three classes of eukaryotic transcription via a unique mechanism</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Trends Biochem Sci</em></journal_title>
                        <volume>38</volume>
                        <author>     Grünberg S, Hahn S</author>
                        <first_page>603</first_page>
                        <last_page>611</last_page>
                        <cYear>2013</cYear>
                        <doi>10.1016/j.tibs.2013.09.002</doi>
                        <article_title>Structural insights into transcription initiation by RNA polymerase II</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Biochim Biophys Acta</em></journal_title>
                        <volume>1861</volume>
                        <author>     Martínezfernández V, Garridogodino AI, Miróngarcía MC, et al.</author>
                        <first_page>1</first_page>
                        <last_page>13</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1016/j.bbagen.2016.11.023</doi>
                        <article_title>Rpb5 modulates the RNA polymerase II transition from initiation to elongation by influencing Spt5 association and backtracking</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nova Science Publishers</em>.</journal_title>
                        <volume></volume>
                        <author>Martínezfernández V, Garridogodino AI, Cuevas-Bermudez A, et al.</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2015</cYear>
                        <doi></doi>
                        <article_title>Cytoplasmic and Nuclear Functions for the Prefoldin-like URI/Bud27</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS Genet</em></journal_title>
                        <volume>9</volume>
                        <author>Miróngarcía MC, Garridogodino AI, Garcíamolinero V, et al.</author>
                        <first_page>e1003297</first_page>
                        <last_page></last_page>
                        <cYear>2013</cYear>
                        <doi>10.1371/journal.pgen.1003297</doi>
                        <article_title>The prefoldin Bud27 mediates the assembly of the eukaryotic RNA polymerases in an Rpb5-dependent manner</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Science</em></journal_title>
                        <volume>302</volume>
                        <author>     Gstaiger M, Luke B, Hess D, et al.</author>
                        <first_page>1208</first_page>
                        <last_page>1212</last_page>
                        <cYear>2003</cYear>
                        <doi>10.1126/science.1088401</doi>
                        <article_title>Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Structure</em></journal_title>
                        <volume>25</volume>
                        <author>     Riveracalzada A, Pal M, Muñozhernández H, et al.</author>
                        <first_page>1145</first_page>
                        <last_page>1152</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1016/j.str.2017.05.016</doi>
                        <article_title>The structure of the R2TP complex defines a platform for recruiting diverse client proteins to the HSP90 molecular chaperone system</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>RNA Biolo</em></journal_title>
                        <volume>9</volume>
                        <author>     Boulon S, Bertrand E, Pradet-Balade B</author>
                        <first_page>148</first_page>
                        <last_page>154</last_page>
                        <cYear>2012</cYear>
                        <doi>10.4161/rna.18494</doi>
                        <article_title>HSP90 and the R2TP co-chaperone complex: Building multi-protein machineries essential for cell growth and gene expression</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell</em></journal_title>
                        <volume>39</volume>
                        <author>     Boulon S, Pradet-Balade B, Verheggen C, et al.</author>
                        <first_page>912</first_page>
                        <last_page>924</last_page>
                        <cYear>2010</cYear>
                        <doi>10.1016/j.molcel.2010.08.023</doi>
                        <article_title>HSP90 and its R2TP/Prefoldin-like cochaperone are involved in the cytoplasmic assembly of RNA polymerase II</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS One</em></journal_title>
                        <volume>8</volume>
                        <author>Mita P, Savas JN, Ha S, et al.</author>
                        <first_page>e63879</first_page>
                        <last_page></last_page>
                        <cYear>2013</cYear>
                        <doi>10.1371/journal.pone.0063879</doi>
                        <article_title>Analysis of URI nuclear interaction with RPB5 and components of the R2TP/Prefoldin-like complex</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nat Commun</em></journal_title>
                        <volume>8</volume>
                        <author>Cloutier P, Poitras C, Durand M, et al.</author>
                        <first_page>15615</first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi>10.1038/ncomms15615</doi>
                        <article_title>R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell Biol</em></journal_title>
                        <volume>25</volume>
                        <author>     Yart A, Gstaiger M, Wirbelauer C, et al.</author>
                        <first_page>5052</first_page>
                        <last_page>5060</last_page>
                        <cYear>2005</cYear>
                        <doi>10.1128/MCB.25.12.5052-5060.2005</doi>
                        <article_title>The HRPT2 tumor suppressor gene product parafibromin associates with human PAF1 and RNA polymerase II</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>elegans. <em>Development</em></journal_title>
                        <volume>133</volume>
                        <author>     Parusel CT, Kritikou EA, Hengartner MO, et al.</author>
                        <first_page>621</first_page>
                        <last_page>629</last_page>
                        <cYear>2006</cYear>
                        <doi></doi>
                        <article_title>URI-1 is required for DNA stability in C</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>BMC Mol Biol</em></journal_title>
                        <volume>9</volume>
                        <author>     Kirchner J, Vissi E, Gross S, et al.</author>
                        <first_page>1</first_page>
                        <last_page>17</last_page>
                        <cYear>2008</cYear>
                        <doi>10.1186/1471-2199-9-1</doi>
                        <article_title>Drosophila Uri, a PP1alpha binding protein, is essential for viability, maintenance of DNA integrity and normal transcriptional activity</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cancer Cell</em></journal_title>
                        <volume>26</volume>
                        <author>     Tummala KS, Gomes AL, Yilmaz M, et al.</author>
                        <first_page>826</first_page>
                        <last_page>839</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1016/j.ccell.2014.10.002</doi>
                        <article_title>Inhibition of De Novo NAD Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cancer Cell</em></journal_title>
                        <volume>30</volume>
                        <author>     Burén S, Gomes AL, Teijeiro A, et al.</author>
                        <first_page>290</first_page>
                        <last_page>307</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1016/j.ccell.2016.06.023</doi>
                        <article_title>Regulation of OGT by URI in Response to Glucose Confers c-MYC-Dependent Survival Mechanisms</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>EMBO J</em></journal_title>
                        <volume>28</volume>
                        <author>     Deplazes A, Mockli N, Luke B, et al.</author>
                        <first_page>1429</first_page>
                        <last_page>1441</last_page>
                        <cYear>2009</cYear>
                        <doi>10.1038/emboj.2009.98</doi>
                        <article_title>Yeast Uri1p promotes translation initiation and may provide a link to cotranslational quality control</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Biol Chem</em></journal_title>
                        <volume>272</volume>
                        <author>     Lin Y, Nomura T, Cheong J, et al.</author>
                        <first_page>7132</first_page>
                        <last_page>7139</last_page>
                        <cYear>1997</cYear>
                        <doi>10.1074/jbc.272.11.7132</doi>
                        <article_title>Hepatitis B virus X protein is a transcriptional modulator that communicates with transcription factor IIB and the RNA polymerase II subunit 5</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell Biol</em></journal_title>
                        <volume>31</volume>
                        <author>     Mita P, Savas JN, Djouder N, et al.</author>
                        <first_page>3639</first_page>
                        <last_page>3652</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1128/MCB.05429-11</doi>
                        <article_title>Regulation of androgen receptor-mediated transcription by RPB5 binding protein URI/RMP</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Biochim Biophys Acta</em></journal_title>
                        <volume>1849</volume>
                        <author>     Vernekar DV, Bhargava P</author>
                        <first_page>1340</first_page>
                        <last_page>1353</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1016/j.bbagrm.2015.09.010</doi>
                        <article_title>Yeast Bud27 modulates the biogenesis of Rpc128 and Rpc160 subunits and the assembly of RNA polymerase III</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell Biol</em></journal_title>
                        <volume>35</volume>
                        <author>     Ciesla M, Makala E, Plonka M, et al.</author>
                        <first_page>1169</first_page>
                        <last_page>1181</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1128/MCB.01230-14</doi>
                        <article_title>Rbs1, a new protein implicated in RNA polymerase III biogenesis in yeast Saccharomyces cerevisiae</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nucleic Acids Res</em></journal_title>
                        <volume>42</volume>
                        <author>     Miróngarcía MC, Garridogodino AI, Martínezfernández V, et al.</author>
                        <first_page>9666</first_page>
                        <last_page>9676</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1093/nar/gku685</doi>
                        <article_title>The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription</article_title>
                    </citation>
                                   </citation_list>
                <keywords language="en"></keywords>
				<abstract language="en"><![CDATA[Rpb5 is one of the five common subunits to all eukaryotic RNA polymerases, which is conserved in archaea, but not in bacteria. Among these common subunits, it is the only one that is not interchangeable between yeasts and humans, and accounts for the functional incompatibility of yeast and human subunits. Rpb5 has been proposed to contribute to the gene-specific activation of RNA pol II, notably during the infectious cycle of the hepatitis B virus, and also to participate in general transcription mediated by all eukaryotic RNA pol. The structural analysis of Rpb5 and its interaction with different transcription factors, regulators and DNA, accounts for Rpb5 being necessary to maintain the correct conformation of the shelf module of RNA pol II, which favors the proper organization of the transcription bubble and the clamp closure of the enzyme.<br />In this work we provide details about subunit Rpb5’s structure, conservation and the role it plays in transcription regulation by analyzing the different interactions with several factors, as well as its participation in the assembly of the three RNA pols, in cooperation with prefoldin-like Bud27/URI.]]></abstract>
			</journal_article>
                        <journal_article>
	            	            <titles>
                    <title><![CDATA[Esophageal cancer research today and tomorrow: Lessons from algae and other perspectives]]></title>
                </titles>
	                            <contributors>
                                </contributors>
                <publication_date media_type="print">
                    <month></month>
                    <year>2018</year>
                </publication_date>
                
                <pages>
                    <first_page>75</first_page>
                    <last_page>90</last_page>
                </pages>
                <doi_data>
                    <doi>10.3934/genet.2018.1.75</doi>
                    <timestamp>2017-12-02 13:00:00.0</timestamp>
					<resource>http://www.aimsciences.org/article/id/1855</resource>
                </doi_data>
	                
                <citation_list>
               	                    <citation key="">
                        <journal_title><em>J Cancer</em></journal_title>
                        <volume>8</volume>
                        <author>Song Q, Jiang D, Wang H, et al.</author>
                        <first_page>2492</first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi>10.7150/jca.19601</doi>
                        <article_title>Chromosomal and genomic variations in esophageal squamous cell carcinoma: A review of technologies, applications, and prospections</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Oncotarget</em></journal_title>
                        <volume>8</volume>
                        <author>         Hou X, Wen J, Ren Z, et al.</author>
                        <first_page>43571</first_page>
                        <last_page>43578</last_page>
                        <cYear>2017</cYear>
                        <doi></doi>
                        <article_title>Non-coding RNAs: New biomarkers and therapeutic targets for esophageal cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title></journal_title>
                        <volume></volume>
                        <author></author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear></cYear>
                        <doi></doi>
                        <article_title>Esophagus Cancer statictics in USA. Esophagus Cancer.org. [Online] June 14, 2017. Available</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Medicinis</em> 4.</journal_title>
                        <volume></volume>
                        <author>Testa U, Castelli G, Pelosi E</author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi></doi>
                        <article_title>Esophageal cancer: Genomic and molecular characterization, stem cell compartment and clonal evolution</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title></journal_title>
                        <volume></volume>
                        <author></author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear></cYear>
                        <doi></doi>
                        <article_title>Esophageal cancer statistics in UK. Cancer Research UK. [Online] Nov 22, 2017. Available</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>World J Gastroenterol</em></journal_title>
                        <volume>19</volume>
                        <author>         Zhang Y</author>
                        <first_page>5598</first_page>
                        <last_page>5606</last_page>
                        <cYear>2013</cYear>
                        <doi>10.3748/wjg.v19.i34.5598</doi>
                        <article_title>Epidemiology of esophageal cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cancer Epidemiol Biomarkers Prev</em></journal_title>
                        <volume>22</volume>
                        <author>         Taylor PR, Abnet CC, Dawsey SM</author>
                        <first_page>540</first_page>
                        <last_page>552</last_page>
                        <cYear>2013</cYear>
                        <doi>10.1158/1055-9965.EPI-12-1347</doi>
                        <article_title>Squamous dysplasia â the precursor lesion for esophageal squamous cell carcinoma</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Semin Radiat Oncol</em></journal_title>
                        <volume>17</volume>
                        <author>         Siewert JR, Ott K</author>
                        <first_page>38</first_page>
                        <last_page>44</last_page>
                        <cYear>2007</cYear>
                        <doi>10.1016/j.semradonc.2006.09.007</doi>
                        <article_title>Are squamous and adenocarcinomas of the esophagus the same disease</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Ebiomedicine</em></journal_title>
                        <volume>8</volume>
                        <author>         Zhao R, Young CB, Mee-Hyun L, et al.</author>
                        <first_page>30</first_page>
                        <last_page>39</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1016/j.ebiom.2016.04.017</doi>
                        <article_title>Implications of genetic and epigenetic alterations of CDKN2A (p16INK4a) in cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em> Am J Surg Pathol</em></journal_title>
                        <volume>39</volume>
                        <author>     Singhi AD, Foxwell TJ, Nason K, et al.</author>
                        <first_page>487</first_page>
                        <last_page>495</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1097/PAS.0000000000000356</doi>
                        <article_title>Smad4 loss in esophageal adenocarcinoma is associated with an increased propensity for disease recurrence and poor survival</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Oncogene</em></journal_title>
                        <volume>33</volume>
                        <author>     Streppel MM, Lata S, DelaBastide M, et al.</author>
                        <first_page>347</first_page>
                        <last_page>357</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1038/onc.2012.586</doi>
                        <article_title>Next-generation sequencing of endoscopic biopsies identifies ARID1A as a tumor-suppressor gene in Barrett's esophagus</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nat Genet</em></journal_title>
                        <volume>45</volume>
                        <author>     Dulak AM, Stojanov P, Peng S, et al.</author>
                        <first_page>478</first_page>
                        <last_page>486</last_page>
                        <cYear>2013</cYear>
                        <doi>10.1038/ng.2591</doi>
                        <article_title>Exome and whole-genome sequencing of esophageal adenocarcinoma identifies recurrent driver events and mutational complexity</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nat Genet</em></journal_title>
                        <volume>46</volume>
                        <author>     Gao YB, Chen ZL, Li JG, et al.</author>
                        <first_page>1097</first_page>
                        <last_page>1102</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1038/ng.3076</doi>
                        <article_title>Genetic landscape of esophageal squamous cell carcinoma</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>World J Gastroenterol</em></journal_title>
                        <volume>22</volume>
                        <author>     Sasaki Y, Tamura M, Koyama R, et al.</author>
                        <first_page>2284</first_page>
                        <last_page>2293</last_page>
                        <cYear>2016</cYear>
                        <doi>10.3748/wjg.v22.i7.2284</doi>
                        <article_title>Genomic characterization of esophageal squamous cell carcinoma: Insights from next-generation sequencing</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS One</em></journal_title>
                        <volume>8</volume>
                        <author>Nazila N, Katrien VR, Mohammad V, et al.</author>
                        <first_page>e73009</first_page>
                        <last_page></last_page>
                        <cYear>2013</cYear>
                        <doi>10.1371/journal.pone.0073009</doi>
                        <article_title>Expression, Tissue Distribution and Function of miR-21 in Esophageal Squamous Cell Carcinoma</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Gastroenterology</em></journal_title>
                        <volume>149</volume>
                        <author>     Rubenstein JH, Shaheen NJ</author>
                        <first_page>302</first_page>
                        <last_page>317</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1053/j.gastro.2015.04.053</doi>
                        <article_title>Epidemiology, diagnosis, and management of esophageal adenocarcinoma</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Cancer Metastasis Treat</em></journal_title>
                        <volume>1</volume>
                        <author>     Watanabe M</author>
                        <first_page>1</first_page>
                        <last_page>7</last_page>
                        <cYear>2015</cYear>
                        <doi></doi>
                        <article_title>Risk factors and molecular mechanisms of esophageal cancer: Differences between the histologic subtypes</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>World J Gastroenterol</em></journal_title>
                        <volume>21</volume>
                        <author>     Halland M, Katzka D, Iyer PG</author>
                        <first_page>6479</first_page>
                        <last_page>6490</last_page>
                        <cYear>2015</cYear>
                        <doi>10.3748/wjg.v21.i21.6479</doi>
                        <article_title>Recent developments in pathogenesis, diagnosis and therapy of Barrett's esophagus</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Dig Dis Sci</em></journal_title>
                        <volume>62</volume>
                        <author>     Palethorpe HM, Drew PA, Smith E</author>
                        <first_page>3402</first_page>
                        <last_page>3414</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1007/s10620-017-4794-5</doi>
                        <article_title>Androgen Signaling in Esophageal Adenocarcinoma Cell Lines <em>In Vitro</em></article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>World J Gastroenterol</em></journal_title>
                        <volume>21</volume>
                        <author>     Wang RH</author>
                        <first_page>5210</first_page>
                        <last_page>5219</last_page>
                        <cYear>2015</cYear>
                        <doi>10.3748/wjg.v21.i17.5210</doi>
                        <article_title>From reflux esophagitis to Barrett's esophagus and esophageal adenocarcinoma</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Oncogene</em></journal_title>
                        <volume>25</volume>
                        <author>     Pietsch EC, Humbey O, Murphy ME</author>
                        <first_page>1602</first_page>
                        <last_page>1611</last_page>
                        <cYear>2006</cYear>
                        <doi>10.1038/sj.onc.1209367</doi>
                        <article_title>Polymorphisms in the p53 pathway</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cell</em></journal_title>
                        <volume>119</volume>
                        <author>     Bond G, Hu W, Bond EE, et al.</author>
                        <first_page>591</first_page>
                        <last_page>602</last_page>
                        <cYear>2004</cYear>
                        <doi>10.1016/j.cell.2004.11.022</doi>
                        <article_title>A single nucleotide polymorphism in the MDM2 promoter</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Hum Genet</em></journal_title>
                        <volume>129</volume>
                        <author>     Akbari M, Malekzadeh R, Lepage P, et al.</author>
                        <first_page>573</first_page>
                        <last_page>582</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1007/s00439-011-0951-7</doi>
                        <article_title>Mutations in Fanconi anemia genes and the risk of esophageal cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Carcinogenesis</em></journal_title>
                        <volume>27</volume>
                        <author>     Dandara C, Li DP, Walther G, et al.</author>
                        <first_page>791</first_page>
                        <last_page>797</last_page>
                        <cYear>2006</cYear>
                        <doi>10.1093/carcin/bgi257</doi>
                        <article_title>Gene-environment interaction: The role of SULT1A1 and CYP3A5 polymorphisms as risk modifiers for squamous cell</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mol Cell Proteomics Mcp</em></journal_title>
                        <volume>16</volume>
                        <author>     O'Neill JR, Pak HS, Pairocastineira E, et al.</author>
                        <first_page>1138</first_page>
                        <last_page>1150</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1074/mcp.M116.065078</doi>
                        <article_title>Quantitative Shotgun Proteomics Unveils Candidate Novel Esophageal Adenocarcinoma (EAC)-Specific Proteins</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Neoplasia</em></journal_title>
                        <volume>19</volume>
                        <author>     Ke X, Yan R, Sun Z, et al.</author>
                        <first_page>941</first_page>
                        <last_page>949</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1016/j.neo.2017.06.007</doi>
                        <article_title>Esophageal Adenocarcinoma-Derived Extracellular Vesicle MicroRNAs Induce a Neoplastic Phenotype in Gastric Organoids</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Oncotarget</em></journal_title>
                        <volume>8</volume>
                        <author>     Lee Y, Urbanska AM, Hayakawa Y, et al.</author>
                        <first_page>203</first_page>
                        <last_page>214</last_page>
                        <cYear>2016</cYear>
                        <doi></doi>
                        <article_title>Gastrin stimulates a cholecystokinin-2-receptor-expressing cardia progenitor cell and promotes progression of Barrett's-like esophagus</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Thorac Dis</em></journal_title>
                        <volume>6</volume>
                        <author>     D'Journo XB, Thomas PA</author>
                        <first_page>S253</first_page>
                        <last_page>S264</last_page>
                        <cYear>2014</cYear>
                        <doi></doi>
                        <article_title>Current management of esophageal cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Ann Thorac Cardiovasc Surg</em></journal_title>
                        <volume>23</volume>
                        <author>     Sohda M, Kuwano H</author>
                        <first_page>1</first_page>
                        <last_page>11</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1093/icvts/ivw098</doi>
                        <article_title>Current Status and Future Prospects for Esophageal Cancer Treatment</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Surg Today</em></journal_title>
                        <volume>45</volume>
                        <author>     Akutsu Y, Matsubara H</author>
                        <first_page>1360</first_page>
                        <last_page>1365</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1007/s00595-015-1116-4</doi>
                        <article_title>Chemoradiotherapy and surgery for T4 esophageal cancer in Japan</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Oncol</em></journal_title>
                        <volume>2010</volume>
                        <author>Sithranga BN, Kathiresan K</author>
                        <first_page>214186</first_page>
                        <last_page></last_page>
                        <cYear>2011</cYear>
                        <doi></doi>
                        <article_title>Anticancer drugs from marine flora: An overview</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Br J Cancer</em></journal_title>
                        <volume>114</volume>
                        <author>     Tebbutt NC, Price TJ, Ferraro DA, et al.</author>
                        <first_page>505</first_page>
                        <last_page>509</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1038/bjc.2015.440</doi>
                        <article_title>Panitumumab added to docetaxel, cisplatin and fluoropyrimidine in oesophagogastric cancer: ATTAX3 phase II trial</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Gastrointest Oncol</em></journal_title>
                        <volume>5</volume>
                        <author>     Niu J, Gelbspan D, Weitz D, et al.</author>
                        <first_page>E103</first_page>
                        <last_page>E110</last_page>
                        <cYear>2014</cYear>
                        <doi></doi>
                        <article_title>HER2-positive, trastuzumab-resistant metastatic esophageal cancer presenting with brain metastasis after durable response to dual HER2 blockade: A case report</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Onkologie</em></journal_title>
                        <volume>35</volume>
                        <author>     Idelevich E, Kashtan H, Klein Y, et al.</author>
                        <first_page>427</first_page>
                        <last_page>431</last_page>
                        <cYear>2012</cYear>
                        <doi>10.1159/000340072</doi>
                        <article_title>Prospective phase II study of neoadjuvant therapy with cisplatin, 5-FU, and bevacizumab for locally advanced resectable esophageal cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>OncoTargets Ther</em></journal_title>
                        <volume>9</volume>
                        <author>     Davidson M, Starling N</author>
                        <first_page>7235</first_page>
                        <last_page>7245</last_page>
                        <cYear>2016</cYear>
                        <doi>10.2147/OTT.S100643</doi>
                        <article_title>Trastuzumab in the management of gastroesophageal cancer: Patient selection and perspectives</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cancer</em></journal_title>
                        <volume>117</volume>
                        <author>     Ilson DH, Kelsen D, Shah M, et al.</author>
                        <first_page>1409</first_page>
                        <last_page>1414</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1002/cncr.25602</doi>
                        <article_title>A phase 2 trial of erlotinib in patients with previously treated squamous cell and adenocarcinoma of the esophagusr</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Thorac Oncol</em></journal_title>
                        <volume>5</volume>
                        <author>     Rodriguez CP, Adelstein DJ, Rice TW, et al.</author>
                        <first_page>229</first_page>
                        <last_page>235</last_page>
                        <cYear>2010</cYear>
                        <doi>10.1097/JTO.0b013e3181c5e334</doi>
                        <article_title>A phase II study of perioperative concurrent chemotherapy, gefitinib, and hyperfractionated radiation followed by maintenance gefitinib in locoregionally advanced esophagus and gastroesophageal junction cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Int J Oncol</em></journal_title>
                        <volume>36</volume>
                        <author>Altiok S, Mezzadra H, Jagannath S, et al.</author>
                        <first_page>19</first_page>
                        <last_page></last_page>
                        <cYear>2010</cYear>
                        <doi></doi>
                        <article_title>A novel pharmacodynamic approach to assess and predict tumor response to the epidermal growth factor receptor inhibitor gefitinib in patients with esophageal cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>World J Gastrointest Pharmacol Ther</em></journal_title>
                        <volume>7</volume>
                        <author>     Martinucci I, Bortoli ND, Russo S, et al.</author>
                        <first_page>190</first_page>
                        <last_page>206</last_page>
                        <cYear>2016</cYear>
                        <doi>10.4292/wjgpt.v7.i2.190</doi>
                        <article_title>Barrett's esophagus in 2016: From pathophysiology to treatment</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Aliment Pharmacol Ther</em></journal_title>
                        <volume>34</volume>
                        <author>     Kwiatek MA, Roman S, Fareeduddin A, et al.</author>
                        <first_page>59</first_page>
                        <last_page>66</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1111/j.1365-2036.2011.04678.x</doi>
                        <article_title>An alginate-antacid formulation (Gaviscon Double Action Liquid) can eliminate the postprandial "acid pocket" in symptomatic GERD patients</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cancer Lett</em></journal_title>
                        <volume>243</volume>
                        <author>     Yuan H, Song J, Li X, et al.</author>
                        <first_page>228</first_page>
                        <last_page>234</last_page>
                        <cYear>2006</cYear>
                        <doi>10.1016/j.canlet.2005.11.032</doi>
                        <article_title>Immunomodulation and antitumor activity of k-carrageenan oligosaccharides</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Ann Oncol</em></journal_title>
                        <volume>13</volume>
                        <author>     Monnerat C, Faivre S, Temam S, et al.</author>
                        <first_page>995</first_page>
                        <last_page>1006</last_page>
                        <cYear>2002</cYear>
                        <doi>10.1093/annonc/mdf172</doi>
                        <article_title>End points for new agents in induction chemotherapy for locally advanced head and neck cancers</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Global Health</em></journal_title>
                        <volume>7</volume>
                        <author>Neergheen-Bhujun V, Awan AT, Baran Y, et al.</author>
                        <first_page>020304</first_page>
                        <last_page></last_page>
                        <cYear>2017</cYear>
                        <doi>10.7189/jogh.07.020304</doi>
                        <article_title>Biodiversity, drug discovery, and the future of global health: Introducing the biodiversity to biomedicine consortium, a call to action</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mar Drugs</em></journal_title>
                        <volume>2</volume>
                        <author>     Jimeno J, Faircloth G, Sousa-Faro JF, et al.</author>
                        <first_page>14</first_page>
                        <last_page>29</last_page>
                        <cYear>2004</cYear>
                        <doi>10.3390/md201014</doi>
                        <article_title>New Marine Derived Anticancer Therapeutics-A Journey from the Sea to Clinical Trials</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em> Mar Drugs</em></journal_title>
                        <volume>2</volume>
                        <author>     Kijjoa A, Sawangwong P</author>
                        <first_page>328</first_page>
                        <last_page>336</last_page>
                        <cYear>2004</cYear>
                        <doi></doi>
                        <article_title>Drugs and Cosmetics from the Sea</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mar Biotechnol</em></journal_title>
                        <volume>13</volume>
                        <author>     Kuo YH, Liang TW, Liu KC, et al.</author>
                        <first_page>451</first_page>
                        <last_page>461</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1007/s10126-010-9316-9</doi>
                        <article_title>Isolation and identification of a novel antioxidant with antitumour activity from Serratia ureilytica using squid pen as fermentation substrate</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Food Chem</em></journal_title>
                        <volume>104</volume>
                        <author>     Kwon HJ, Bae SY, Kim KH, et al.</author>
                        <first_page>196</first_page>
                        <last_page>201</last_page>
                        <cYear>2007</cYear>
                        <doi>10.1016/j.foodchem.2006.11.031</doi>
                        <article_title>Induction of apoptosis in HeLa cells by ethanolic extract of Corallina pilulifera</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Appl Toxicol  Jat</em></journal_title>
                        <volume>29</volume>
                        <author>     Lins KOAL, Bezerra DP, Alves APNN, et al.</author>
                        <first_page>20</first_page>
                        <last_page>26</last_page>
                        <cYear>2009</cYear>
                        <doi>10.1002/jat.1374</doi>
                        <article_title>Antitumor properties of a sulfated polysaccharide from the red seaweed Champia feldmannii (Diaz-Pifferer)</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>World J Hepatol</em></journal_title>
                        <volume>3</volume>
                        <author>     Thoppil RJ, Bishayee A</author>
                        <first_page>228</first_page>
                        <last_page>249</last_page>
                        <cYear>2011</cYear>
                        <doi>10.4254/wjh.v3.i9.228</doi>
                        <article_title>Terpenoids as potential chemopreventive and therapeutic agents in liver cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Eur Rev Med Pharmacol Sci</em></journal_title>
                        <volume>14</volume>
                        <author>     Zandi K, Ahmadzadeh S, Tajbakhsh S, et al.</author>
                        <first_page>669</first_page>
                        <last_page>673</last_page>
                        <cYear>2010</cYear>
                        <doi></doi>
                        <article_title>Anticancer activity of Sargassum oligocystum water extract against human cancer cell lines</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Carbohydr Polym</em></journal_title>
                        <volume>175</volume>
                        <author>     Usoltseva RV, Anastyuk SD, Shevchenko NM, et al.</author>
                        <first_page>547</first_page>
                        <last_page>556</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1016/j.carbpol.2017.08.044</doi>
                        <article_title>Polysaccharides from brown algae Sargassum duplicatum: The structure and anticancer activity <em>in vitro</em></article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Springerplus</em></journal_title>
                        <volume>5</volume>
                        <author>Alves C, Pinteus S, Horta A, et al.</author>
                        <first_page>1339</first_page>
                        <last_page></last_page>
                        <cYear>2016</cYear>
                        <doi>10.1186/s40064-016-2938-2</doi>
                        <article_title>High cytotoxicity and anti-proliferative activity of algae extracts on an <em>in vitro</em> model of human hepatocellular carcinoma</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Food Chem Toxicol</em></journal_title>
                        <volume>47</volume>
                        <author>     Gamal-Eldeen AM, Ahmed EF, Abo-Zeid MA</author>
                        <first_page>1378</first_page>
                        <last_page>1384</last_page>
                        <cYear>2009</cYear>
                        <doi>10.1016/j.fct.2009.03.016</doi>
                        <article_title><em>In vitro</em> cancer chemopreventive properties of polysaccharide extract from the brown alga, Sargassum latifolium</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Nat Prod</em></journal_title>
                        <volume>74</volume>
                        <author>     Foley SA, Mulloy B, Tuohy MG, et al.</author>
                        <first_page>1851</first_page>
                        <last_page>1861</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1021/np200124m</doi>
                        <article_title>An unfractionated fucoidan from Ascophyllum nodosum: Extraction, characterization, and apoptotic effects <em>in vitro</em></article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Biosci Bioeng</em></journal_title>
                        <volume>110</volume>
                        <author>     Jiang Z, Okimura T, Yokose T</author>
                        <first_page>113</first_page>
                        <last_page>117</last_page>
                        <cYear>2010</cYear>
                        <doi>10.1016/j.jbiosc.2010.01.007</doi>
                        <article_title>Effects of sulfated fucan, ascophyllan, from the brown Alga Ascophyllum nodosum on various cell lines: A comparative study on ascophyllan and fucoidan</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>PLoS One</em></journal_title>
                        <volume>6</volume>
                        <author>Zhang Z, Kiichiro T, Hiroshi E, et al.</author>
                        <first_page>e27441</first_page>
                        <last_page></last_page>
                        <cYear>2011</cYear>
                        <doi>10.1371/journal.pone.0027441</doi>
                        <article_title>Fucoidan extract induces apoptosis in MCF-7 cells via a mechanism involving the ROS-dependent JNK activation and mitochondria-mediated pathways</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mar Drugs</em></journal_title>
                        <volume>9</volume>
                        <author>     Jiao G, Yu G, Zhang J, et al.</author>
                        <first_page>196</first_page>
                        <last_page>223</last_page>
                        <cYear>2011</cYear>
                        <doi>10.3390/md9020196</doi>
                        <article_title>Chemical structures and bioactivities of sulfated polysaccharides from marine algae</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Glycobiology</em></journal_title>
                        <volume>17</volume>
                        <author>     Cumashi A, Ushakova NA, Preobrazhenskaya ME, et al.</author>
                        <first_page>541</first_page>
                        <last_page>552</last_page>
                        <cYear>2007</cYear>
                        <doi>10.1093/glycob/cwm014</doi>
                        <article_title>A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Biol Pharm Bull</em></journal_title>
                        <volume>32</volume>
                        <author>     Hyun J, Kim S, Kang J, et al.</author>
                        <first_page>1760</first_page>
                        <last_page>1764</last_page>
                        <cYear>2009</cYear>
                        <doi>10.1248/bpb.32.1760</doi>
                        <article_title>Apoptosis inducing activity of fucoidan in HCT-15 colon carcinoma cells</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Food  Sci</em></journal_title>
                        <volume>76</volume>
                        <author>     Park HS, Kim GY, Nam TJ, et al.</author>
                        <first_page>T77</first_page>
                        <last_page>T83</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1111/j.1750-3841.2011.02099.x</doi>
                        <article_title>Antiproliferative activity of fucoidan was associated with the induction of apoptosis and autophagy in AGS human gastric cancer cells</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Sci Rep</em></journal_title>
                        <volume>5</volume>
                        <author>Luo M, Shao B, Nie W, et al.</author>
                        <first_page>11062</first_page>
                        <last_page></last_page>
                        <cYear>2015</cYear>
                        <doi>10.1038/srep11062</doi>
                        <article_title>Antitumor and Adjuvant Activity of λ-carrageenan by Stimulating Immune Response in Cancer Immunotherapy</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Iran J Cancer Prev</em></journal_title>
                        <volume>9</volume>
                        <author>Jazzara M, Ghannam A, Soukkarieh C, et al.</author>
                        <first_page>e3836</first_page>
                        <last_page></last_page>
                        <cYear>2016</cYear>
                        <doi></doi>
                        <article_title>Anti-Proliferative Activity of  λ-Carrageenan Through the Induction of Apoptosis in Human Breast Cancer Cells</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Appl Phycol</em></journal_title>
                        <volume>23</volume>
                        <author>     Yuan Y, Song J, Li X, et al.</author>
                        <first_page>59</first_page>
                        <last_page>65</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1007/s10811-010-9536-4</doi>
                        <article_title>Enhanced immunostimulatory and antitumor activity of different derivatives of kappa-carrageenan oligosaccharides from Kappaphycus striatum</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Mar Drugs</em></journal_title>
                        <volume>11</volume>
                        <author>     Fedorov SN, Ermakova SP, Zvyagintseva TN, et al.</author>
                        <first_page>4876</first_page>
                        <last_page>4901</last_page>
                        <cYear>2013</cYear>
                        <doi>10.3390/md11124876</doi>
                        <article_title>Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Carbohydr Polym</em></journal_title>
                        <volume>87</volume>
                        <author>     Yermak IM, Barabanova AO, Aminin DL, et al.</author>
                        <first_page>713</first_page>
                        <last_page>720</last_page>
                        <cYear>2012</cYear>
                        <doi>10.1016/j.carbpol.2011.08.053</doi>
                        <article_title>Effects of structural peculiarities of carrageenans on their immunomodulatory and anticoagulant activities</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Appl  Phycol</em></journal_title>
                        <volume>28</volume>
                        <author>     Kalitnik AA, Anastyuk SD, Sokolova EV, et al.</author>
                        <first_page>545</first_page>
                        <last_page>553</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1007/s10811-015-0577-6</doi>
                        <article_title>Oligosaccharides of k/β-carrageenan from the red alga Tichocarpus crinitus and their ability to induce interleukin 10</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Agents Actions</em></journal_title>
                        <volume>11</volume>
                        <author>     Thomson AW, Fowler EF</author>
                        <first_page>265</first_page>
                        <last_page>273</last_page>
                        <cYear>1981</cYear>
                        <doi>10.1007/BF01967625</doi>
                        <article_title>Carrageenan: A review of its effects on the immune system</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Inflammation Res</em></journal_title>
                        <volume>62</volume>
                        <author>     Morais-Zani KD, Nunes FPB, Silva JBD, et al.</author>
                        <first_page>733</first_page>
                        <last_page>742</last_page>
                        <cYear>2013</cYear>
                        <doi>10.1007/s00011-013-0628-x</doi>
                        <article_title>The anti-inflammatory action of Bothrops jararaca snake antithrombin on acute inflammation induced by carrageenan in mice</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em> Clin Exp Allergy</em></journal_title>
                        <volume>33</volume>
                        <author>     Tsuji R, Hoshino K, Noro Y, et al.</author>
                        <first_page>249</first_page>
                        <last_page>258</last_page>
                        <cYear>2003</cYear>
                        <doi>10.1046/j.1365-2222.2003.01575.x</doi>
                        <article_title>Suppression of allergic reaction by λ-carrageenan: Toll-like receptor 4/MyD88-dependent and -independent modulation of immunity</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Controlled Release</em></journal_title>
                        <volume>40</volume>
                        <author>     Garcia AM, Chaly ES</author>
                        <first_page>179</first_page>
                        <last_page>186</last_page>
                        <cYear>1996</cYear>
                        <doi>10.1016/0168-3659(95)00179-4</doi>
                        <article_title>Preliminary spherical agglomerates of water-soluble drug using natural polymer and cross-linking technique</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Eur J Pharm Biopharm</em></journal_title>
                        <volume>56</volume>
                        <author>     Schmidt AG, Wartewig S, Picker KM</author>
                        <first_page>101</first_page>
                        <last_page>110</last_page>
                        <cYear>2003</cYear>
                        <doi>10.1016/S0939-6411(03)00037-7</doi>
                        <article_title>Potential of carrageenans to protect drugs from polymorphic transformation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title>I. Influence of type and fraction of filler. <em>Eur J Pharm Biopharm</em></journal_title>
                        <volume>63</volume>
                        <author>     Thommes M, Kleinebudde P</author>
                        <first_page>59</first_page>
                        <last_page>67</last_page>
                        <cYear>2006</cYear>
                        <doi></doi>
                        <article_title>Use of kappa-carrageenan as alternative pelletisation aid to microcrystalline cellulose in extrusion/spheronisation</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Pharm Sci</em></journal_title>
                        <volume>90</volume>
                        <author>     Hugerth AM</author>
                        <first_page>1665</first_page>
                        <last_page>1677</last_page>
                        <cYear>2001</cYear>
                        <doi>10.1002/jps.1117</doi>
                        <article_title>Micropolarity and microviscosity of amitriptyline and dextran sulfate/carrageenan-amitriptyline systems: The nature of polyelectrolyte-drug complexes</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Pharm  Sci</em></journal_title>
                        <volume>89</volume>
                        <author>     Patil RT, Speaker TJ</author>
                        <first_page>9</first_page>
                        <last_page>15</last_page>
                        <cYear>2000</cYear>
                        <doi>10.1002/(SICI)1520-6017(200001)89:1<9::AID-JPS2>3.0.CO;2-5</doi>
                        <article_title>Water-based microsphere delivery system for proteins</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Recent Adv Mar Biotechnol</em></journal_title>
                        <volume>9</volume>
                        <author>     Yermak IM, Khotimchenko YS</author>
                        <first_page>207</first_page>
                        <last_page>255</last_page>
                        <cYear>2003</cYear>
                        <doi></doi>
                        <article_title>Chemical properties, biological activities and applications of carrageenan from red algae</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Carbohydr Polym</em></journal_title>
                        <volume>101</volume>
                        <author>Volod'ko AV, Davydova VN, Chusovitin E, et al.</author>
                        <first_page>1087</first_page>
                        <last_page></last_page>
                        <cYear>2014</cYear>
                        <doi>10.1016/j.carbpol.2013.10.049</doi>
                        <article_title>Soluble chitosan carrageenan polyelectrolyte complexes and their gastroprotective activity</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Sci Aliment</em></journal_title>
                        <volume>17</volume>
                        <author>     Lahaye M, Kaeffer B</author>
                        <first_page>563</first_page>
                        <last_page>584</last_page>
                        <cYear>1997</cYear>
                        <doi></doi>
                        <article_title>Seaweed dietary fibres: Structure, physico-chemical and biological properties relevent to intestinal physiology</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em> Lancet</em></journal_title>
                        <volume>373</volume>
                        <author>             Cummings JH, Mann JI, Nishida C, et al.</author>
                        <first_page>365</first_page>
                        <last_page>366</last_page>
                        <cYear>2009</cYear>
                        <doi>10.1016/S0140-6736(09)60117-3</doi>
                        <article_title>Dietary fibre: An agreed definition</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cancer  Res</em></journal_title>
                        <volume>63</volume>
                        <author>     Harris RE, Chlebowski RT, Jackson RD, et al.</author>
                        <first_page>6096</first_page>
                        <last_page>6101</last_page>
                        <cYear>2003</cYear>
                        <doi></doi>
                        <article_title>Breast cancer and nonsteroidal  anti-inflammatory drugs: Prospective results from the Women's Health Initiative</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title></journal_title>
                        <volume></volume>
                        <author></author>
                        <first_page></first_page>
                        <last_page></last_page>
                        <cYear></cYear>
                        <doi></doi>
                        <article_title>Stansbury, Jillian, SEAWEED, CHEWING GUM AND GERD. Ndnr Botanical Medicine, [Online] January 17, 2017. Available</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Prostaglandins Other Lipid Mediators</em></journal_title>
                        <volume>96</volume>
                        <author>     Greene ER, Huang S, Serhan CN, et al.</author>
                        <first_page>27</first_page>
                        <last_page>36</last_page>
                        <cYear>2011</cYear>
                        <doi>10.1016/j.prostaglandins.2011.08.004</doi>
                        <article_title>Regulation of inflammation in cancer by eicosanoids</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nature</em></journal_title>
                        <volume>420</volume>
                        <author>     Coussens LM, Werb Z</author>
                        <first_page>860</first_page>
                        <last_page>867</last_page>
                        <cYear>2002</cYear>
                        <doi>10.1038/nature01322</doi>
                        <article_title>Inflammation and cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nat Rev Immunol</em></journal_title>
                        <volume>4</volume>
                        <author>     Vakkila J, Lotze MT</author>
                        <first_page>641</first_page>
                        <last_page>648</last_page>
                        <cYear>2004</cYear>
                        <doi>10.1038/nri1415</doi>
                        <article_title>Inflammation and necrosis promote tumour growth</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>N Engl J Med</em></journal_title>
                        <volume>323</volume>
                        <author>     Ekbom A, Helmick C, Zack M, et al.</author>
                        <first_page>1228</first_page>
                        <last_page>1233</last_page>
                        <cYear>1990</cYear>
                        <doi>10.1056/NEJM199011013231802</doi>
                        <article_title>Ulcerative colitis and colorectal cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Surg Oncol</em></journal_title>
                        <volume>101</volume>
                        <author>     Kulaylat MN, Dayton MT</author>
                        <first_page>706</first_page>
                        <last_page>712</last_page>
                        <cYear>2010</cYear>
                        <doi>10.1002/jso.21505</doi>
                        <article_title>Ulcerative colitis and cancer</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Carbohydr Polym</em></journal_title>
                        <volume>115</volume>
                        <author>     Kalitnik AA, Marcov PA, Anastyuk SD, et al.</author>
                        <first_page>768</first_page>
                        <last_page>775</last_page>
                        <cYear>2015</cYear>
                        <doi>10.1016/j.carbpol.2014.04.070</doi>
                        <article_title>Gelling polysaccharide from Chondrus armatus and its oligosaccharides: The structural peculiarities and anti-inflammatory</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Biomed Mater Res Part A</em></journal_title>
                        <volume>104</volume>
                        <author>     Sokolova EV, Karetin Y, Davydova VN, et al.</author>
                        <first_page>1603</first_page>
                        <last_page>1609</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1002/jbm.a.35693</doi>
                        <article_title>Carrageenans effect on neutrophils alone and in combination with LPS <em>in vitro</em></article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Curr Opin Pharmacol</em></journal_title>
                        <volume>9</volume>
                        <author>     Abdel-Latif MM, Duggan S, Reynolds JV, et al.</author>
                        <first_page>396</first_page>
                        <last_page>404</last_page>
                        <cYear>2009</cYear>
                        <doi>10.1016/j.coph.2009.06.010</doi>
                        <article_title>Inflammation and esophageal carcinogenesis</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Cancer Epidemiol Biomarkers Prev</em></journal_title>
                        <volume>23</volume>
                        <author>     Hardikar S, Onstad L, Song X, et al.</author>
                        <first_page>2393</first_page>
                        <last_page>2403</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1158/1055-9965.EPI-14-0384</doi>
                        <article_title>Inflammation and oxidative stress markers and esophageal adenocarcinoma incidence in a Barrett's esophagus cohort</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Recent Pat Food Nutr Agric</em></journal_title>
                        <volume>6</volume>
                        <author>     Wang Q, Hao J, Guan Q, et al.</author>
                        <first_page>23</first_page>
                        <last_page>26</last_page>
                        <cYear>2014</cYear>
                        <doi>10.2174/2212798406666141024111945</doi>
                        <article_title>The Mediterranean diet and gastrointestinal cancers risk</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>J Am Coll Nutr</em></journal_title>
                        <volume>31</volume>
                        <author>     Jessri M, Rashidkhani B, Hajizadeh B, et al.</author>
                        <first_page>338</first_page>
                        <last_page>351</last_page>
                        <cYear>2012</cYear>
                        <doi>10.1080/07315724.2012.10720437</doi>
                        <article_title>Adherence to Mediterranean-style dietary pattern and risk of esophageal squamous cell carcinoma: A case-control study in Iran</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Nat Methods</em></journal_title>
                        <volume>13</volume>
                        <author>             Caliari SR, Burdick JA</author>
                        <first_page>405</first_page>
                        <last_page>414</last_page>
                        <cYear>2016</cYear>
                        <doi>10.1038/nmeth.3839</doi>
                        <article_title>A practical guide to hydrogels for cell culture</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Stem Cell Rep</em></journal_title>
                        <volume>3</volume>
                        <author>     Rybtsov S, Batsivari A, Bilotkach K, et al.</author>
                        <first_page>489</first_page>
                        <last_page>501</last_page>
                        <cYear>2014</cYear>
                        <doi>10.1016/j.stemcr.2014.07.009</doi>
                        <article_title>Tracing the origin of the HSC hierarchy reveals an SCF-dependent, IL-3-independent CD43- embryonic precursor</article_title>
                    </citation>
                                       <citation key="">
                        <journal_title><em>Chem Soc Rev</em></journal_title>
                        <volume>46</volume>
                        <author>     Huang Q, Zou Y, Arno MC, et al.</author>
                        <first_page>6255</first_page>
                        <last_page>6275</last_page>
                        <cYear>2017</cYear>
                        <doi>10.1039/C6CS00052E</doi>
                        <article_title>Hydrogel scaffolds for differentiation of adipose-derived stem cells</article_title>
                    </citation>
                                   </citation_list>
                <keywords language="en"></keywords>
				<abstract language="en"><![CDATA[Esophageal cancer is an increasing concern due to poor prognosis, aggressive disease modalities, and a lack of efficient therapeutics. The two types of esophageal cancer: esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC) are responsible for an estimated 450,000 annual deaths, with over 457,000 new patients diagnosed in 2015, making it the eighth most prevalent and the 10th most fatal cancer worldwide. As esophageal cancer prevalence continues to increase, and so does the pressing need for the development of new and effective strategies for the early diagnostics, prevention, and treatment of this cancer, as well for building the innovative research tools to understand the affected molecular mechanisms.<br />    This short review summarizes the current statistics and recent research of the problems and solutions related to the esophageal cancer, and offer a brief overview of its epidemiology, molecular alterations, and existing biomedical tools. We will discuss currently available research tools and discuss selected approaches we deem relevant to find new model systems and therapies for the future with the special focus on novel opportunities presented by the unique molecules found in algae, namely carbohydrates and lipids. Their remarkable chemical variability is connected to their striking structural and functional properties, which combined with the relative novelty of these compounds to cancer biology, warrants interest of the wide biomedical community to these molecules, especially in the esophageal cancer theory and practice.]]></abstract>
			</journal_article>
            		</journal>
	</body>
</doi_batch>