AIMS Energy, 2015, 3(2): 201-213. doi: 10.3934/energy.2015.2.201.

Research article

Export file:


  • RIS(for EndNote,Reference Manager,ProCite)
  • BibTex
  • Text


  • Citation Only
  • Citation and Abstract

Ethanol fermentation by the thermotolerant yeast, Kluyveromyces marxianus TISTR5925, of extracted sap from old oil palm trunk

1 Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
2 Forestry and Forest Products Research Institute (FFPRI), 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan;
3 Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor Darul Ehsan, Malaysia;
4 Universiti Sains Malaysia (USM), 11800 USM, Penang, Malaysia;
5 Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50 Chatuchak, Bangkok 10900, Thailand

Palm sap extracted from old oil palm trunks was previously found to contain sugar and nutrients (amino acids and vitamins). Some palm saps contain a low content of sugar due to differences in species or in plant physiology. Here we condensed palm sap with a low content of sugar using flat membrane filtration, then fermented the condensed palm sap at high temperature using the thermotolerant, high ethanol-producing yeast, Kluyveromyces marxianus. Ethanol production under non-optimum conditions was evaluated. Furthermore, the energy required to concentrate the palm sap, and the amount of energy that could be generated from the ethanol, was calculated. The condensation of sugar in sap from palm trunk required for economically viable ethanol production was evaluated.
  Article Metrics

Keywords Elaeis guineensis; oil palm trunk; waste; bioethanol; thermotolerant yeast; Kluyveromyces marxianus

Citation: Yoshinori Murata, Hatairat Danjarean, Kiyohiko Fujimoto, Akihiko Kosugi, Takamitsu Arai, Wan Asma Ibrahim, Othman Suliman, Rokiah Hashim, Yutaka Mori. Ethanol fermentation by the thermotolerant yeast, Kluyveromyces marxianus TISTR5925, of extracted sap from old oil palm trunk. AIMS Energy, 2015, 3(2): 201-213. doi: 10.3934/energy.2015.2.201


  • 1 Ismail A, Mamat MN (2002) The Optimal Age Of Oil Palm Replanting. Oil Palm Industry Economic J 2:11-18.
  • 2 Basiron Y, Weng CK (2006) Oil palm: the agricultural producer of food, fiber, and fuel for global economy. Oil Palm Industry Economic J 8:1-17.
  • 3 Baker ES, Sahri MH, H'ng PS (2008) Anatomical characteristics and utilization of oil palm wood, In The formation of wood in tropical forest trees—A challenge from the perspective of functional wood anatomy. Editors: Nobuchi T. and Sahri MH. Penerbit Universiti Putra Malaysia, Serdang. Chapter12: 161-180.
  • 4 Sulaiman O, Hashim R, Wahab R, et al. (2008) Evaluation on some finishing properties of oil palm plywood. Holz Roh Werkst 66: 5-10.
  • 5 Yamada H, Tanaka R, Sulaiman O, et al. (2010) Old oil palm trunk: A promising source of sugars for bioethanol production. Biomass Bioenerg 34: 1608-1613.
  • 6 Kosugi A, Tanaka R, Magara K, et al. (2010) Ethanol and lactic acid production using sap squeezed from old oil palm trunks felled for replanting. J Biosci Bioeng 110: 322-325.
  • 7 Kitagawa T, Tokuhiro K, Sugiyama H, et al. (2010) Construction of a beta-glucosidase expression system using the multistress-tolerant yeast Issatchenkia orientalis. Appl Microbiol Biotechnol 87: 1841-1853.
  • 8 Saithong P, Nakamura T, Shima J (2009) Prevention of bacterial contamination using acetate-tolerant Schizosaccharomyces pombe during bioethanol production from molasses. J Biosci Bioeng 108: 216-219.
  • 9 Limtong S, Sringiew C, Yongmanitchai W (2007) Production of fuel ethanol at high temperature from sugar cane juice by a newly isolated Kluyveromyces marxianus. Bioresour Technol 98: 3367-3374.
  • 10 Nonklang S, Abdel-Banat BM, Cha-aim K, et al. (2008) High-temperature ethanol fermentation and transformation with linear DNA in the thermotolerant yeast Kluyveromyces marxianus DMKU3-1042. Appl Environ Microbiol 74: 7514-7521.
  • 11 Abdel-Banat BM, Hoshida H, Ano A, et al. (2010) High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast? Appl Microbiol Biotechnol 85:861-867.
  • 12 Apiwatanapiwat W, Vaithanomsat P, Rugthaworn P, et al. (2013) Ethanol production from cassava pulp by a newly isolated Kluyveromyces marxianus TISTR 5925 strain at high temperature. AIMS Energy 1: 3-16.
  • 13 Murata Y, Tanaka R, Fujimoto K, et al. (2013) Development of sap compressing systems from oil palm trunk. Biomass Bioenergy 51: 8-16.
  • 14 Apiwatanapiwat W, Murata Y, Kosugi A, et al. (2011) Direct ethanol production from cassava pulp using a surface-engineered yeast strain co-displaying two amylases, two cellulases, and beta-glucosidase. Appl Microbiol Biotechnol 90: 377-384.
  • 15 Obahiagnon FI, Osagie AU (2007) Sugar and macrominerals composition of sap produced by Raphia hookeri palms. African J Biotechn 6: 744-750.
  • 16 Eze MO, Ogan AU (1988) Sugars of the unfermented sap and the wine from the oil palm, Elaeis guinensis, tree. Plant Food Human Nutrition 38: 121-126.
  • 17 Singh R, Malhotra SP (2000) Carbon fixation, sucrose synthesis and its transport to storage tissues. Carbonhydrate Reserves in Plants—Synthesis and Regulation 1: 1-34
  • 18 Heldt HW, Chon CJ, Maronde D, et al. (1977) Role of orthophosphate and other factors in the regulation of starch formation in leaves and isolated chloroplasts. Plant Physiology 59: 1146-1155.
  • 19 Kamahara H, Hasanudin U, Widiyanto A, et al. (2010) Improvement potential for net energy balance of biodiesel derived from palm oil: A case study from Indonesian practice. Biomass Bioenergy 34: 1818-1824.


This article has been cited by

  • 1. Shweta Priya, Rameshwar Tiwari, Sarika Rana, Mohanram Saritha, Surender Singh, Anju Arora, Lata Nain, Saccharification of biopretreated paddy straw with indigenous holocellulase and fermentation with Saccharomyces cerevisiae LN1 under optimized conditions, Energy, Ecology and Environment, 2016, 10.1007/s40974-016-0021-z
  • 2. Jairam Choudhary, Surender Singh, Lata Nain, Thermotolerant fermenting yeasts for simultaneous saccharification and fermentation of lignocellulosic biomass, Electronic Journal of Biotechnology, 2016, 10.1016/j.ejbt.2016.02.007
  • 3. Vinay Sharma, A Review on Current Technological Advancement of Lignocellulosic Bioethanol Production, Journal of Applied Biotechnology & Bioengineering, 2016, 1, 2, 10.15406/jabb.2016.01.00011
  • 4. Rameshwar Avchar, Marizeth Groenewald, Abhishek Baghela,  Wickerhamiella shivajii sp. nov., a thermotolerant yeast isolated from distillery effluent, International Journal of Systematic and Evolutionary Microbiology, 2019, 10.1099/ijsem.0.003616

Reader Comments

your name: *   your email: *  

Copyright Info: 2015, Yoshinori Murata, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (

Download full text in PDF

Export Citation

Copyright © AIMS Press All Rights Reserved