Assessment of phylogenetic diversity and relationships among <i>Grewia tenax</i> population in Sudan using RAPD and ISSR molecular markers

Rami S. Habeballa; Mutasim Mohamed Khalafalla Ali; Rashied S. Modawi; Rami S. Habeballa; Mutasim Mohamed Khalafalla Ali; Rashied S. Modawi

doi:10.3934/molsci.2022001

AIMS Molecular Science

2022, Volume 9, Issue 1: 1-24. doi: 10.3934/molsci.2022001

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Research article

Assessment of phylogenetic diversity and relationships among Grewia tenax population in Sudan using RAPD and ISSR molecular markers

1.
Department of Basic Science, Deanship of preparatory year, Prince Satam bin Abdulaziz University, Al Kharj, Kingdom of Saudi Arabia
2.
Department of Environmental Health, College of Public Health and Health Informatics, Umm Al -Qura University, Kingdom of Saudi Arabia
3.
Department of Biology and Biotechnology, Faculty of Science and Technology, Al Neelain University, Sudan

Received: 02 December 2021 Revised: 13 February 2022 Accepted: 15 February 2022 Published: 28 February 2022

This research is conducted to study the genetic diversity of the genus Grewia tenax in Sudan. This plant has a common vernacular Sudanese Arabic name: “Gudeim”. It is mainly cultivated in North Darfur and Western Sudan. Common uses of Grewia species were overviewed in areas of nutrition, folk medicine and famine food. The genetic variation and genetic relationships among Grewia spp populations from different regions were efficiently determined using RAPD and ISSR markers.

The results of genetic analysis were statistically analyzed by STATISTCA and GenALEx 6.5 software. The results of molecular variance revealed that 74% of total genetic diversity was due to within populations variations as opposed to 26% due to variations between populations unweighted pair- group method with arithmetic average (UPGMA) were constructed for and RAPD + ISSR. The UPGMA results showed variability of Grewia genotypes.

It was concluded that both the marker systems RAPD and ISSR combination can be effectively used in determination of genetic relationships among Grewia tenax genotypes.
- Grewia tenax,
- genetic diversity,
- RAPD and ISSR,
- GenAlEx,
- Unweighted Pair group method with arithmetic mean (UPGMA)
Citation: Rami S. Habeballa, Mutasim Mohamed Khalafalla Ali, Rashied S. Modawi. Assessment of phylogenetic diversity and relationships among Grewia tenax population in Sudan using RAPD and ISSR molecular markers[J]. AIMS Molecular Science, 2022, 9(1): 1-24. doi: 10.3934/molsci.2022001

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Abstract

This research is conducted to study the genetic diversity of the genus Grewia tenax in Sudan. This plant has a common vernacular Sudanese Arabic name: “Gudeim”. It is mainly cultivated in North Darfur and Western Sudan. Common uses of Grewia species were overviewed in areas of nutrition, folk medicine and famine food. The genetic variation and genetic relationships among Grewia spp populations from different regions were efficiently determined using RAPD and ISSR markers.

The results of genetic analysis were statistically analyzed by STATISTCA and GenALEx 6.5 software. The results of molecular variance revealed that 74% of total genetic diversity was due to within populations variations as opposed to 26% due to variations between populations unweighted pair- group method with arithmetic average (UPGMA) were constructed for and RAPD + ISSR. The UPGMA results showed variability of Grewia genotypes.

It was concluded that both the marker systems RAPD and ISSR combination can be effectively used in determination of genetic relationships among Grewia tenax genotypes.

Acknowledgments

The authors would like to extend his appreciation to the Deanship of Scientific Research at Prince Satam Bin Abdulaziz University, Alkharj, Kingdom of Saudi Arabia for support and encouragement.

Conflict of interest

The authors declare that there is no conflict of interest regarding the publication of this article.

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