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Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg protein

  • Received: 01 October 2015 Accepted: 12 November 2015 Published: 16 November 2015
  • Ionizing radiations induce clustered damage sites known to be severely challenging for the cell's repair machinery. In the present study, we have grafted on a biochip four synthetic oligonucleotide duplexes containing either a single 8-oxoguanine, a single 2'-deoxyribose abasic site or two different clustered damages containing these two lesions on opposite strands. Using a SPR imaging-based DNA array, we qualitatively observed the hierarchy of lesion processing by the base excision repair (BER) enzyme Fpg (Formamidopyrimidine (fapy)-DNA glycosylase). We could notably show that Fpg can convert a cluster of lesions into a potentially lethal double strand break (DSB) and demonstrate that SPR imaging is suitable to investigate the incision steps occurring during the repair process.

    Citation: Muriel Jourdan, Julia Pingel, Arnaud Buhot, Thierry Livache, Jean-François Constant. Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg protein[J]. AIMS Materials Science, 2015, 2(4): 473-483. doi: 10.3934/matersci.2015.4.473

    Related Papers:

  • Ionizing radiations induce clustered damage sites known to be severely challenging for the cell's repair machinery. In the present study, we have grafted on a biochip four synthetic oligonucleotide duplexes containing either a single 8-oxoguanine, a single 2'-deoxyribose abasic site or two different clustered damages containing these two lesions on opposite strands. Using a SPR imaging-based DNA array, we qualitatively observed the hierarchy of lesion processing by the base excision repair (BER) enzyme Fpg (Formamidopyrimidine (fapy)-DNA glycosylase). We could notably show that Fpg can convert a cluster of lesions into a potentially lethal double strand break (DSB) and demonstrate that SPR imaging is suitable to investigate the incision steps occurring during the repair process.


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