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Structural studies of T4S systems by electron microscopy

  • Received: 20 March 2015 Accepted: 21 May 2015 Published: 15 June 2015
  • Type IV secretion (T4S) systems are large dynamic nanomachines that transport DNA and/or proteins through the membranes of bacteria. Analysis of T4S system architecture is an extremely challenging task taking into account their multi protein organisation and lack of overall global symmetry. Nonetheless the last decade demonstrated an amazing progress achieved by X-ray crystallography and cryo-electron microscopy. In this review we present a structural analysis of this dynamic complex based on recent advances in biochemical, biophysical and structural studies.

    Citation: Adam Redzej, Gabriel Waksman, Elena V Orlova. Structural studies of T4S systems by electron microscopy[J]. AIMS Biophysics, 2015, 2(2): 184-199. doi: 10.3934/biophy.2015.2.184

    Related Papers:

  • Type IV secretion (T4S) systems are large dynamic nanomachines that transport DNA and/or proteins through the membranes of bacteria. Analysis of T4S system architecture is an extremely challenging task taking into account their multi protein organisation and lack of overall global symmetry. Nonetheless the last decade demonstrated an amazing progress achieved by X-ray crystallography and cryo-electron microscopy. In this review we present a structural analysis of this dynamic complex based on recent advances in biochemical, biophysical and structural studies.


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