Review

Basics of dentin-pulp tissue engineering

  • Received: 03 June 2018 Accepted: 18 September 2018 Published: 25 September 2018
  • Regeneration is reconstruction of a tissue with the shape and function of the original tissue including vascularization and innervations. Highly degradation of dentin-pulp complex can not be reversed by its own repair mechanisms. For decades, endodontic treatments including pulpectomy and preparation of root canals have been the first choice for these cases. However, root canal treatment method has some unsatisfying consequences like; esthetic problems as a conclusion of discoloration caused by endodontic filling materials, undermined integrity of tooth structure, postoperative fractures, coronal leakage or periapical microleakage, lost sense of environmental changes which can make the recurrent caries or apical infection unnoticeable for patient and shortened lifetime in comparison with vital teeth. Currently regeneration of dentin-pulp complex by tissue engineering approach is thought to be a more appropriate choice instead of root canal treatment according to these outcomes. In this review, we will discuss basic components as stem cells, signaling molecules and scaffolds and also methods used for dentin-pulp tissue engineering.

    Citation: Izgen Karakaya, Nuran Ulusoy. Basics of dentin-pulp tissue engineering[J]. AIMS Bioengineering, 2018, 5(3): 162-178. doi: 10.3934/bioeng.2018.3.162

    Related Papers:

  • Regeneration is reconstruction of a tissue with the shape and function of the original tissue including vascularization and innervations. Highly degradation of dentin-pulp complex can not be reversed by its own repair mechanisms. For decades, endodontic treatments including pulpectomy and preparation of root canals have been the first choice for these cases. However, root canal treatment method has some unsatisfying consequences like; esthetic problems as a conclusion of discoloration caused by endodontic filling materials, undermined integrity of tooth structure, postoperative fractures, coronal leakage or periapical microleakage, lost sense of environmental changes which can make the recurrent caries or apical infection unnoticeable for patient and shortened lifetime in comparison with vital teeth. Currently regeneration of dentin-pulp complex by tissue engineering approach is thought to be a more appropriate choice instead of root canal treatment according to these outcomes. In this review, we will discuss basic components as stem cells, signaling molecules and scaffolds and also methods used for dentin-pulp tissue engineering.


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    [1] Kitagawa M, Ueda H, Iizuka S, et al. (2007) Immortalization and characterization of human dental pulp cells with odontoblastic differentiation. Arch Oral Biol 52: 727–731. doi: 10.1016/j.archoralbio.2007.02.006
    [2] Nakashima M (2005) Bone morphogenetic proteins in dentin regeneration for potential use in endodontic therapy. Cytokine Growth F R 16: 369–376. doi: 10.1016/j.cytogfr.2005.02.011
    [3] Tziafas D, Smith AJ, Lesot H (2000) Designing new treatment strategies in vital pulp therapy. J Dent 28: 77–92. doi: 10.1016/S0300-5712(99)00047-0
    [4] Cooper PR, Takahashi Y, Graham LW, et al. (2010) Inflammation-regeneration interplay in the dentine-pulp complex. J Dent 38: 687–697. doi: 10.1016/j.jdent.2010.05.016
    [5] Smith AJ, Murray PE, Sloan AJ, et al. (2001) Trans-dentinal stimulation of tertiary dentinogenesis. Adv Dent Res 15: 51–54. doi: 10.1177/08959374010150011301
    [6] Goldberg M (2011) Pulp healing and regeneration: More questions than answers. Adv Dent Res 23: 270–274. doi: 10.1177/0022034511405385
    [7] Arana-Chavez VE, Massa LF (2004) Odontoblast: The cells forming and maintaining dentine. Int J Biochem Cell B 36: 1367–1373. doi: 10.1016/j.biocel.2004.01.006
    [8] About I (2013) Dentin-pulp regeneration: The primordial role of the microenvironment and its modification by traumatic injuries and bioactive materials. Endod Top 28: 61–89. doi: 10.1111/etp.12038
    [9] Smith AJ, Scheven BA, Takahashi Y. et al. (2012) Dentine as a bioactive extracellular matrix. Arch Oral Biol 57: 109–121. doi: 10.1016/j.archoralbio.2011.07.008
    [10] Mitsiadis TA, Feki A, Papaccio G, et al. (2011) Dental pulp stem cells, niches and notch signaling in tooth injury. Adv Dent Res 23: 275–279. doi: 10.1177/0022034511405386
    [11] Sharma LA, Sharma A, Dias GJ (2015) Advances in regeneration of dental pulp-a literature review. J Invest Clin Dent 6: 85–98. doi: 10.1111/jicd.12064
    [12] Yu T, Volponi AA, Babb R, et al. (2015) Stem cells in tooth development, growth, repair, and regeneration. Curr Top Dev Biol 115: 187–212. doi: 10.1016/bs.ctdb.2015.07.010
    [13] Hashemi-Beni B, Khoroushi M, Foroughi MR, et al. (2017) Tissue engineering: Dentin-pulp complex regeneration approaches (A review). Tissue Cell 49: 552–564. doi: 10.1016/j.tice.2017.07.002
    [14] Cassidy N, Fahey M, Prime SS, et al. (1997) Comparative analysis of transforming growth factor-β isoforms 1–3 in human and rabbit dentine matrices. Arch Oral Biol 42: 219–223. doi: 10.1016/S0003-9969(96)00115-X
    [15] Eramo S, Natali A, Pinna R, et al. (2018) Dental pulp regeneration via cell homing. Int Endod J 51: 405–419. doi: 10.1111/iej.12868
    [16] Huang GTJ (2009) Pulp and dentin tissue engineering and regeneration: Current progress. Regen Med 4: 697–707.
    [17] Galler KM, Souza RND, Hartgerink JD, et al. (2011) Scaffolds for dental pulp tissue engineering. Adv Dent Res 23: 333–339. doi: 10.1177/0022034511405326
    [18] Rombouts C, Giraud T, Jeanneau C, et al. (2017) Pulp vascularization during tooth development, regeneration, and therapy. J Dent Res 96: 137–144. doi: 10.1177/0022034516671688
    [19] Rosa V (2013) What and where are the stem cells for dentistry? Singapore Dent J 34: 13–18. doi: 10.1016/j.sdj.2013.11.003
    [20] Nussenbaum B, Krebsbach PH (2006) The role of gene therapy for craniofacial and dental tissue engineering. Adv Drug Deliver Rev 58: 577–591.
    [21] Neel EAA, Chrzanowski W, Salih VM, et al. (2014) Tissue engineering in dentistry. J Dent 42: 915–928. doi: 10.1016/j.jdent.2014.05.008
    [22] Gong T, Heng BC, Lo ECM, et al. (2016) Current advance and future prospects of tissue engineering approach to dentin/pulp regenerative therapy. Stem Cells Int 2016: 1–13.
    [23] Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126: 663–676. doi: 10.1016/j.cell.2006.07.024
    [24] Ozeki N, Hase N, Yamaguchi H, et al. (2015) Polyphosphate induces matrix metalloproteinase-3-mediated proliferation of odontoblast-like cells derived from induced pluripotent cells. Exp Cell Res 333: 303–315. doi: 10.1016/j.yexcr.2015.01.007
    [25] Xie H, Dubey N, Shim W, et al. (2018) Functional odontoblastic-like cells derived from human iPSCs. J Dent Res 97: 77–83. doi: 10.1177/0022034517730026
    [26] Ishizaka R, Iohara K, Murakami M, et al. (2012) Regeneration of dental pulp following pulpectomy by fractionated stem/progenitor cells from bone marrow and adipose tissue. Biomaterials 33: 2109–2118. doi: 10.1016/j.biomaterials.2011.11.056
    [27] Hung CN, Mar K, Chang HC, et al. (2011) A comparison between adipose tissue and dental pulp as sources of MSCs for tooth regeneration. Biomaterials 32: 6995–7005. doi: 10.1016/j.biomaterials.2011.05.086
    [28] Ricucci D, Loghin S, Lin LM, et al. (2014) Is hard tissue formation in the dental pulp after the death of the primary odontoblasts a regenerative or a reparative process? J Dent 42: 1156–1170. doi: 10.1016/j.jdent.2014.06.012
    [29] Kawashima N (2012) Characterization of dental pulp stem cells: A new horizon for tissue regeneration? Arch Oral Biol 57: 1439–1458. doi: 10.1016/j.archoralbio.2012.08.010
    [30] Liu J, Yu F, Sun Y, et al. (2015) Concise reviews: Characteristics and potential applications of human dental tissue‐derived mesenchymal stem cells. StemCells 33: 627–638.
    [31] Egusa H, Sonoyama W, Nishimura M, et al. (2012) Stem cells in dentistry-part I: Stem cell sources. J Prosthodont Res 56: 151–165. doi: 10.1016/j.jpor.2012.06.001
    [32] Cantore S, Ballini A, De DV, et al. (2017) Characterization of human apical papilla-derived stem cells. J Biol Regul Homeostic Agents 31: 901–910.
    [33] Aksel H, Öztürk Ş, Serper A, et al. (2018) VEGF/BMP-2 loaded three-dimensional model for enhanced angiogenic and odontogenic potential of dental pulp stem cells. Int Endod J 51: 420–430. doi: 10.1111/iej.12869
    [34] Liang Z, Kawano S, Chen W, et al. (2018) Minced pulp as source of pulpal mesenchymal stem cells with odontogenic differentiation capacity. J Endod 44: 80–86.
    [35] Mayo V, Sawatari Y, Huang CYC, et al. (2014) Neural crest-derived dental stem cells-where we are and where we are going. J Dent 42: 1043–1051. doi: 10.1016/j.jdent.2014.04.007
    [36] Khayat A, Monteiro N, Smith EE, et al. (2017) GeIMA-encapsulated hDPSCs and HUVECs for dental pulp regeneration. J Dent Res 96: 192–199. doi: 10.1177/0022034516682005
    [37] Dissanayaka WL, Zhu L, Hargreaves KM, et al. (2014) Scaffold-free prevascularized microtissue spheroids for pulp regeneration. J Dent Res 93: 1296–1303. doi: 10.1177/0022034514550040
    [38] Bakopoulou A, Leyhausen G, Volk J, et al. (2011) Comparative analysis of in vitro osteo-odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAP). Arch Oral Biol 56: 709–721. doi: 10.1016/j.archoralbio.2010.12.008
    [39] Wang X, Sha XJ, Li GH, et al. (2012) Comparative characterization of stem cells from human exfoliated deciduous teeth and dental pulp stem cells. Arch Oral Biol 57: 1231–1240. doi: 10.1016/j.archoralbio.2012.02.014
    [40] Yang B, Chen G, Li J, et al. (2012) Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix-based scaffold. Biomaterials 33: 2449–2461. doi: 10.1016/j.biomaterials.2011.11.074
    [41] Saito MT, Silvério KG, Casati MZ, et al. (2015) Tooth-derived stem cells: Update and perspectives. World J Stem Cell 7: 399–407. doi: 10.4252/wjsc.v7.i2.399
    [42] Roberts-Clark DJ, Smith AJ (2000) Angiogenic growth factors in human dentine matrix. Arch Oral Biol 45: 1013–1016. doi: 10.1016/S0003-9969(00)00075-3
    [43] Bakopoulou A, Leyhausen G, Volk J, et al. (2015) Wnt/β-catenin signaling regulates Dental Pulp Stem Cells' responses to pulp injury by resinous monomers. Dent Mater 31: 542–555. doi: 10.1016/j.dental.2015.02.004
    [44] Liu N, Zhou M, Zhang Q, et al. (2018) Stiffness regulates the proliferation and osteogenic/odontogenic differentiation of human dental pulp stem cells via the WNT signaling pathway. CellProliferat 51: e12345.
    [45] Balic A, Thesleff I (2015) Tissue interactions regulating tooth development and renewal. Curr Top Dev Biol 115: 157–186. doi: 10.1016/bs.ctdb.2015.07.006
    [46] Qin W, Liu P, Zhang R, et al. (2014) JNK MAPK is involved in BMP-2-induced odontoblastic differentiation of human dental pulp cells. Connect Tissue Res 55: 217–224. doi: 10.3109/03008207.2014.882331
    [47] Lee SK, Lee CY, Kook YA, et al. (2010) Mechanical stress promotes odontoblastic differentiation via the heme oxygenase-1 pathway in human dental pulp cell line. Life Sci 86: 107–114. doi: 10.1016/j.lfs.2009.11.013
    [48] Zhang M, Jiang F, Zhang X, et al. (2017) The effects of platelet‐derived growth factor‐BB on human dental pulp stem cells mediated dentin‐pulp complex regeneration. Stem Cells Trans Med 6: 2126–2134. doi: 10.1002/sctm.17-0033
    [49] Sloan AJ, Rutherford RB, Smith AJ (2000) Stimulation of the rat dentine-pulp complex by bone morphogenetic protein-7 in vitro. Arch Oral Biol 45: 173–177. doi: 10.1016/S0003-9969(99)00131-4
    [50] Six N, Lasfagues JJ, Goldberg M (2002) Differential repair responses in the coronal and radicular areas of the exposed rat molar pulp induced by recombinant human bone morphogenetic protein 7 (osteogenic protein 1). Arch Oral Biol 47: 177–187. doi: 10.1016/S0003-9969(01)00100-5
    [51] Magloire H, Romeas A, Melin M, et al. (2001) Molecular regulation of odontoblast activity under dentin injury. Adv Dent Res 15: 46–50. doi: 10.1177/08959374010150011201
    [52] Sloan AJ, Smith AJ (1999) Stimulation of the dentin-pulp complex of rat incisor teeth by transforming growth factor-β isoforms 1-3 in vitro. Arch Oral Biol 44: 149–156. doi: 10.1016/S0003-9969(98)00106-X
    [53] Malik Z, Alexiou M, Hallgrimsson B, et al. (2018) Bone morphogenetic protein 2 coordinates early tooth mineralization. J Dent Res 97: 835–843. doi: 10.1177/0022034518758044
    [54] Almushayt A, Narayanan K, Zaki AE, et al. (2006) Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts. Gene Ther 13: 611–620. doi: 10.1038/sj.gt.3302687
    [55] About I, Mitsiadis TA (2001) Molecular aspects of tooth pathogenesis and repair: In vivo and in vitro models. Adv Dent Res 15: 59–62. doi: 10.1177/08959374010150011501
    [56] Vidovic-Zdrilic I, Vining KH, Vijaykumar A, et al. (2018) FGF2 enhances odontoblast differentiation by αSMA+ progenitors in vivo. J Dent Res 97: 1170–1177. doi: 10.1177/0022034518769827
    [57] Srisuwan T, Tilkorn DJ, Al-Benna S, et al. (2012) Survival of rat functional dental pulp cells in vascularized tissue engineering chambers. Tissue Cell 44: 111–121. doi: 10.1016/j.tice.2011.12.003
    [58] Onishi T, Kinoshita S, Shintani S, et al. (1999) Stimulation of proliferation and differentiation of dog dental pulp cells in serum-free culture medium by insulin-like growth factor. Arch Oral Biol 44: 361–371. doi: 10.1016/S0003-9969(99)00007-2
    [59] Matsumura S, Quispe-Salcedo A, Schiller CM, et al. (2017) IGF-I mediates EphrinB1 activation in regulating tertiary dentin formation. J Dent Res 96: 1153–1161. doi: 10.1177/0022034517708572
    [60] Nowwarote N, Pavasant P, Osathanon T (2015) Role of endogenous basic fibroblast growth factor in stem cells isolated from human exfoliated deciduous teeth. Arch Oral Biol 60: 403–415.
    [61] Nowwarote N, Sukarawan W, Pavasant P, et al. (2017) Basic fibroblast growth factor regulates rex1 expression via il‐6 in stem cells isolated from human exfoliated deciduous teeth. J Cell Biochem 118: 1480–1488. doi: 10.1002/jcb.25807
    [62] Tran-Hung L, Laurent P, Camps J, et al. (2008) Quantification of angiogenic growth factors released by human dental cells after injury. Arch Oral Biol 53: 9–13. doi: 10.1016/j.archoralbio.2007.07.001
    [63] Aksel H, Huang GTJ (2017) Human and swine dental pulp stem cells form a vascularlike network after angiogenic differentiation in comparison with endothelial cells: A quantitative analysis. J Endod 43: 588–595. doi: 10.1016/j.joen.2016.11.015
    [64] Caviedes‐Bucheli J, Gomez‐Sosa JF, Azuero‐Holguin MM, et al. (2017) Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma. Int Endod J 50: 339–351. doi: 10.1111/iej.12627
    [65] Jun H, Lei D, Qifang Y, et al. (2018) Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: An in vitro study. Braz Oral Res 32: e48.
    [66] Kim MK, Park HJ, Kim YD, et al. (2014) Hinokitiol increases the angiogenic potential of dental pulp cells through ERK and p38MAPK activation and hypoxia-inducible factor-1α (HIF-1α) upregulation. Arch Oral Biol 59: 102–110. doi: 10.1016/j.archoralbio.2013.10.009
    [67] Kuang R, Zhang Z, Jin X, et al. (2016) Nanofibrous spongy microspheres for the delivery of hypoxia-primed human dental pulp stem cells to regenerate vascularized dental pulp. Acta Biomater 33: 225–234. doi: 10.1016/j.actbio.2016.01.032
    [68] Duncan HF, Smith AJ, Fleming GJP, et al. (2011) HDACi: Cellular effects, opportunities for restorative dentistry. J Dent Res 90: 1377–1388. doi: 10.1177/0022034511406919
    [69] Bento LW, Zhang Z, Imai A, et al. (2013) Endothelial differentiation of SHED requires MEK1/ERK signaling. J Dent Res 92: 51–57. doi: 10.1177/0022034512466263
    [70] Nakashima M, Iohara K, Sugiyama M (2009) Human dental pulp stem cells with highly angiogenic and neurogenic potential for possible use in pulp regeneration. Cytokine Growth F R 20: 435–440. doi: 10.1016/j.cytogfr.2009.10.012
    [71] de Vicente JC, Cabo R, Ciriaco E, et al. (2002) Impaired dental cytodifferentiation in glial cell-line derived growth factor (GDNF) deficient mice. Ann Anat 184: 85–92. doi: 10.1016/S0940-9602(02)80041-3
    [72] Murakami M, Horibe H, Iohara K, et al. (2013) The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential. Biomaterials 34: 9036–9047. doi: 10.1016/j.biomaterials.2013.08.011
    [73] Stokowski A, Shi S, Sun T, et al. (2007) EphB/Ephrin‐B interaction mediates adult stem cell attachment, spreading, and migration: Implications for dental tissue repair. Stem Cells 25: 156–164. doi: 10.1634/stemcells.2006-0373
    [74] Heng BC, Wang S, Gong T, et al. (2018) EphrinB2 signaling enhances osteogenic/odontogenic differentiation of human dental pulp stem cells. Arch Oral Biol 87: 62–71 doi: 10.1016/j.archoralbio.2017.12.014
    [75] Yang JW, Zhang YF, Wan CY, et al. (2015) Autophagy in SDF-1α-mediated DPSC migration and pulp regeneration. Biomaterials 44: 11–23. doi: 10.1016/j.biomaterials.2014.12.006
    [76] Petit I, Jin D, Rafii S (2007) The SDF-1–CXCR4 signaling pathway: A molecular hub modulating neo-angiogenesis. Trends Immunol 28: 299–307. doi: 10.1016/j.it.2007.05.007
    [77] Guang LG, Boskey AL, Zhu W (2013) Age-related CXC chemokine receptor-4-deficiency impairs osteogenic differentiation potency of mouse bone marrow mesenchymal stromal stem cells. Int J Biochem Cell Biol 45: 1813–1820. doi: 10.1016/j.biocel.2013.05.034
    [78] Yun HM, Kang SK, Singh RK, et al. (2016) Magnetic nanofiber scaffold-induced stimulation of odontogenesis and pro-angiogenesis of human dental pulp cells through Wnt/MAPK/NF-κB pathways. Dent Mater 32: 1301–1311. doi: 10.1016/j.dental.2016.06.016
    [79] Zheng L, Yang F, Shen H, et al. (2011) The effect of composition of calcium phosphate composite scaffolds on the formation of tooth tissue from human dental pulp stem cells. Biomaterials 32: 7053–7059. doi: 10.1016/j.biomaterials.2011.06.004
    [80] Hu L, Gao Z, Xu J, et al. (2017) Decellularized swine dental pulp as a bioscaffold for pulp regeneration. BioMed Res Int 2017: 1–9.
    [81] Chen L, Liu L, Wu C, et al. (2017) The extracts of bredigite bioceramics enhanced the pluripotency of human dental pulp cells. J Biomed Mater Res A 105: 3465–3474.
    [82] Chen J, Cui C, Qiao X, et al. (2017) Treated dentin matrix paste as a novel pulp capping agent for dentin regeneration. J Tissue Eng Regen M 11: 3428–3436. doi: 10.1002/term.2256
    [83] Wang J, Liu X, Jin X, et al. (2010) The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. Acta Biomater 6: 3856–3863. doi: 10.1016/j.actbio.2010.04.009
    [84] Khoroushi M, Foroughi MR, Karbasi S, et al. (2018) Effect of Polyhydroxybutyrate/Chitosan/Bioglass nanofiber scaffold on proliferation and differentiation of stem cells from human exfoliated deciduous teeth into odontoblast-like cells. Mater Sci Eng C 89: 128–139. doi: 10.1016/j.msec.2018.03.028
    [85] Lee W, Oh JH, Park JC, et al. (2012) Performance of electrospun poly (ε-caprolactone) fiber meshes used with mineral trioxide aggregates in a pulp capping procedure. Acta Biomater 8: 2986–2995. doi: 10.1016/j.actbio.2012.04.032
    [86] Paduano F, Marrelli M, White LJ, et al. (2016) Odontogenic differentiation of human dental pulp stem cells on hydrogel scaffolds derived from decellularized bone extracellular matrix and collagen type I. PLoS One 11: 1–18.
    [87] Atalayin C, Tezel H, Dagci T, et al. (2016) In vivo performance of different scaffolds for dental pulp stem cells induced for odontogenic differentiation. Braz Oral Res 30: 1–7.
    [88] Wang J, Ma H, Jin X, et al. (2011) The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells. Biomaterials 32: 7822–7830. doi: 10.1016/j.biomaterials.2011.04.034
    [89] Dissanayaka WL, Zhu L, Hargreaves KM, et al. (2015) In vitro analysis of scaffold-free prevascularized microtissue spheroids containing human dental pulp cells and endothelial cells. J Endod 41: 663–670. doi: 10.1016/j.joen.2014.12.017
    [90] Huang GTJ, Al‐Habib M, Gauthier P (2013) Challenges of stem cell‐based pulp and dentin regeneration: A clinical perspective. Endod Top 28: 51–60. doi: 10.1111/etp.12035
    [91] Park H, Choi B, Hu J, et al. (2013) Injectable chitosan hyaluronic acid hydrogels for cartilage tissue engineering. ActaBiomater 9: 4779–4786.
    [92] Li X, Ma C, Xie X, et al. (2016) Pulp regeneration in a full-length human tooth root using a hierarchical nanofibrous microsphere system. ActaBiomater 35: 57–67.
    [93] Huang GTJ, Yamaza T, Shea LD, et al. (2010) Stem/progenitor cell-mediated de novo regeneration of dental pulp with newly deposited continuous layer of dentin in an in vivo model. Tissue Eng Part A 16: 605–615. doi: 10.1089/ten.tea.2009.0518
    [94] Galler KM, Eidt A, Schmalz G (2014) Cell-free approaches for dental pulp tissue engineering. J Endod 40: S41–S45. doi: 10.1016/j.joen.2014.01.014
    [95] Kim SG, Zhou J, Ye L, et al. (2012) Regenerative endodontics: Barriers and strategies for clinical translation. Dent ClinNorth Am 56: 639–649.
    [96] Kim SG, Zheng Y, Zhou J, et al. (2013) Dentin and dental pulp regeneration by the patient's endogenous cells. Endod Top 28: 106–117. doi: 10.1111/etp.12037
    [97] Zhang LX, Shen LL, Ge SH, et al. (2015) Systemic BMSC homing in the regeneration of pulp-like tissue and the enhancing effect of stromal cell-derived factor-1 on BMSC homing. Int J Clin Exp Pathol 8: 10261–10271.
    [98] Yang J, Yuan G, Chen Z (2016) Pulp regeneration: Current approaches and future challenges. Front Physiol 7: 58.
    [99] Alongi DJ, Yamaza T, Song Y, et al. (2010) Stem/progenitor cells from inflamed human dental pulp retain tissue regeneration potential. Regen Med 5: 617–631. doi: 10.2217/rme.10.30
    [100] Kim JY, Xin X, Moioli EK, et al. (2010) Regeneration of dental-pulp-like tissue by chemotaxis-induced cell homing. Tissue Eng Part A 16: 3023–3031. doi: 10.1089/ten.tea.2010.0181
    [101] Torabinejad M, Turman M (2011) Revitalization of tooth with necrotic pulp and open apex by using platelet-rich plasma: A case report. J Endod 37: 265–268. doi: 10.1016/j.joen.2010.11.004
    [102] Mitsiadis TA, Woloszyk A (2015) Odyssey of human dental pulp stem cells and their remarkable ability to survive in extremely adverse conditions. Front Physiol 6: 1–2.
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