Influence of heat shock proteins in individual sensitivity of human neutrophils to heat stress

Viktor Semenkov; Anatolii Mikhalskii; Alexandr Sapoznikov; Viktor Semenkov; Anatolii Mikhalskii; Alexandr Sapoznikov

doi:10.3934/molsci.2019.2.38

AIMS Molecular Science

2019, Volume 6, Issue 2: 38-51. doi: 10.3934/molsci.2019.2.38

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Research article

Influence of heat shock proteins in individual sensitivity of human neutrophils to heat stress

1.
Russian Medical University N. I. Pirogov, Ostrovitianov Street 1, 117997 Moscow, Russia
2.
V. A. Trapeznikov Institute of Control Sciences RAS, Profsoyuznaya 65, 117997 Moscow, Russia
3.
Institute of Bioorganic Chemistry RAS, Mikluho-Maklaia 16/10, 117997 Moscow, Russia

Received: 26 March 2019 Accepted: 19 July 2019 Published: 01 August 2019

We have developed a simple and reliable method to measure the sensitivity of individuals to oxidative stress. This method utilizes luminol-amplified chemiluminescence to quantify production of reactive oxygen species (ROS) by opsonized zymosan-stimulated neutrophils that have been subjected to short-term stress via heat shock. In this study, the chemiluminescence reaction was used to monitor the dynamics of ROS production in neutrophils derived from 17 patients of different ages and genders before and after these neutrophils were subjected to heat shock. In addition, we determined expression of Toll-like receptors using fluorescent-labeled antibody. The effects of adrenaline, dexamethasone, aspirin, and indomethacin, as well as different doses of exogenous heat shock protein 70 (Hsp70), on the production of ROS by stimulated neutrophils was also investigated. Our data showed that adrenaline and exogenous Hsp70 both suppressed ROS production by stimulated neutrophils. Furthermore, TLR4 expression was upregulated upon heat stress. Thus, adrenaline, HSPs, and TLRs may all play a role in regulating stress responses in phagocytes.
- heat shock,
- chemiluminescence of neutrophils,
- heat shock protein 70 (Hsp70),
- individual sensitivity,
- Toll-like receptors
Citation: Viktor Semenkov, Anatolii Mikhalskii, Alexandr Sapoznikov. Influence of heat shock proteins in individual sensitivity of human neutrophils to heat stress[J]. AIMS Molecular Science, 2019, 6(2): 38-51. doi: 10.3934/molsci.2019.2.38

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Abstract

We have developed a simple and reliable method to measure the sensitivity of individuals to oxidative stress. This method utilizes luminol-amplified chemiluminescence to quantify production of reactive oxygen species (ROS) by opsonized zymosan-stimulated neutrophils that have been subjected to short-term stress via heat shock. In this study, the chemiluminescence reaction was used to monitor the dynamics of ROS production in neutrophils derived from 17 patients of different ages and genders before and after these neutrophils were subjected to heat shock. In addition, we determined expression of Toll-like receptors using fluorescent-labeled antibody. The effects of adrenaline, dexamethasone, aspirin, and indomethacin, as well as different doses of exogenous heat shock protein 70 (Hsp70), on the production of ROS by stimulated neutrophils was also investigated. Our data showed that adrenaline and exogenous Hsp70 both suppressed ROS production by stimulated neutrophils. Furthermore, TLR4 expression was upregulated upon heat stress. Thus, adrenaline, HSPs, and TLRs may all play a role in regulating stress responses in phagocytes.

Abbreviation ROS: reactive oxygen species; TLR: Toll-like receptor; Hsp70: heat shock protein 70; LPS: lipopolysaccharide; IL-1: interleukin 1; TNF: tumor necrosis factor;

Conflict of interest

The authors declare no conflict of interest.

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