Sensitivity of <em>Deinococcus grandis rodZ</em> deletion mutant to calcium ions results in enhanced spheroplast size

Yusuke Morita; Mai Okumura; Issay Narumi; Hiromi Nishida; Yusuke Morita; Mai Okumura; Issay Narumi; Hiromi Nishida

doi:10.3934/microbiol.2019.2.176

AIMS Microbiology

2019, Volume 5, Issue 2: 176-185. doi: 10.3934/microbiol.2019.2.176

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Sensitivity of Deinococcus grandis rodZ deletion mutant to calcium ions results in enhanced spheroplast size

1.
Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan
2.
Radiation Microbiology Laboratory, Department of Life Sciences, Faculty of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura, Gunma 374-0193, Japan

Received: 04 April 2019 Accepted: 24 June 2019 Published: 26 June 2019

RodZ is a cytoskeletal protein associated with bacterial cell shape. It is a transmembrane protein located on the plasma membrane, and it binds to another cytoskeletal protein MreB. Deinococcus grandis contains a rodZ homolog. Although D. grandis is rod-shaped, it becomes spherical in shape when the rodZ homolog is disrupted. The rodZ deletion mutant was treated with lysozyme to generate spheroplasts. The spheroplasts enlarged in medium containing calcium chloride and penicillin. The rodZ deletion mutant spheroplasts were more sensitive to calcium ions than wild type. Cell and cytoplasm sizes of enlarged spheroplasts of the rodZ deletion mutant tended to be larger than those of wild type. Thus, disruption of rodZ enhances plasma and outer membrane expansion in D. grandis spheroplasts.
- cell size,
- cytoplasm size,
- Deinococcus grandis,
- rod shape,
- rodZ deletion mutant,
- sensitivity to calcium ion,
- spherical shape,
- spheroplast enlargement
Citation: Yusuke Morita, Mai Okumura, Issay Narumi, Hiromi Nishida. Sensitivity of Deinococcus grandis rodZ deletion mutant to calcium ions results in enhanced spheroplast size[J]. AIMS Microbiology, 2019, 5(2): 176-185. doi: 10.3934/microbiol.2019.2.176

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Abstract

RodZ is a cytoskeletal protein associated with bacterial cell shape. It is a transmembrane protein located on the plasma membrane, and it binds to another cytoskeletal protein MreB. Deinococcus grandis contains a rodZ homolog. Although D. grandis is rod-shaped, it becomes spherical in shape when the rodZ homolog is disrupted. The rodZ deletion mutant was treated with lysozyme to generate spheroplasts. The spheroplasts enlarged in medium containing calcium chloride and penicillin. The rodZ deletion mutant spheroplasts were more sensitive to calcium ions than wild type. Cell and cytoplasm sizes of enlarged spheroplasts of the rodZ deletion mutant tended to be larger than those of wild type. Thus, disruption of rodZ enhances plasma and outer membrane expansion in D. grandis spheroplasts.

Acknowledgments

We thank Rintaro Tsuchikado for his experiment assistance. This work was funded by JSPS KAKENHI Grant Numbers 16K14891 (to HN) and 17K07730 (to IN).

Conflict of Interest

The authors declare that there is no conflict of interest regarding the publication of this paper.

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