Export file:


  • RIS(for EndNote,Reference Manager,ProCite)
  • BibTex
  • Text


  • Citation Only
  • Citation and Abstract

Hydrolytic enzyme activity enhanced by Barium supplementation

1 Center for Technological and Scientific Research in Mining (CICITEM) and Biotechnology Department, University of Antofagasta, Avda. José Miguel Carrera 1701, Antofagasta, Chile
2 Institute La Grasa, Spanish National Research Council (IG-CSIC), Avda. Padre García Tejero 4, 41012 Sevilla, Spain
3 Institute of Natural Resources and Agrobiology, Spanish National Research Council (IRNAS-CSIC), Avda. Reina Mercedes 10, 41012 Sevilla, Spain

Topical Section: Microorganisms in sustainable agriculture and biotechnology

Hydrolysis of polymers is a first and often limiting step during the degradation of plant residues. Plant biomass is generally a major component of waste residues and a major renewable resource to obtain a variety of secondary products including biofuels. Improving the performance of enzymatic hydrolysis of plant material with minimum costs and limiting the use of additional microbial biomass or hydrolytic enzymes directly influences competitiveness of these green biotechnological processes. In this study, we cloned and expressed a cellulase and two esterases recovered from environmental thermophilic soil bacterial communities and characterize their optimum activity conditions including the effect of several metal ions. Results showed that supplementing these hydrolytic reactions with Barium increases the activity of these extracellular hydrolytic enzymes. This observation represents a simple but major improvement to enhance the efficiency and competitiveness of this process within an increasingly important biotechnological sector.
  Article Metrics

Keywords barium; extracellular enzyme activity; hydrolytic activity; cellulose; esterase

Citation: Camilo Muñoz, Fernando G. Fermoso, Mariella Rivas, Juan M. Gonzalez. Hydrolytic enzyme activity enhanced by Barium supplementation. AIMS Microbiology, 2016, 2(4): 402-411. doi: 10.3934/microbiol.2016.4.402


  • 1. Sexton S, Zilberman D, Rajagopal D, et al. (2009) The role of biotechnology in a sustainable biofuel future. AgBioForum 12: 130–140.
  • 2. Bhalla A, Bansal N, Kumar S, et al. (2013) Improved lignocellulose conversion to biofuels with thermophilic bacteria and thermostable enzymes. Bioresour Technol 128: 751–759.
  • 3. Horn SJ, Vaaje-Kolstad G, Westereng B, et al. (2012) Novel enzymes for the degradation of cellulose. Biotechnol Biofuels 5: 89–93.
  • 4. Shubert R, Schellnhuber HJ, Buchmann N, et al. (2010) Future bioenergy and sustainable land use. German Advisory Council on Global Change. London: Earthscan.
  • 5. Kein-Marcuschamer D, Oleskowicz-Popiel P, Simmons PA, et al. (2012) The challenge of enzyme cost in the production of lignocellulosic biofuels. Biotechnol Bioeng 109: 1083–1087.
  • 6. Jeffries TW (1990) Biodegradation of lignin-carbohydrate complexes. Biodegradation 1: 163–176.
  • 7. Fulekar MH (2010) Environmental Biotechnology, Boca raton, Florida: CRC Press, 308.
  • 8. Li Q, Du W, Liu D (2008) Perspectives of microbial oils for biodiesel production. Appl Microbiol Biotechnol 80: 749–756.
  • 9. Zandvoort MH, van Hullebush ED, Fermoso FG, et al. (2006) Trace metals in anaerobic granular sludge reactors: bioavailability and dosing strategies. Eng Life Sci 6: 293–301.
  • 10. Portillo MC, Reina M, Serrano L, et al. (2008) Role of specific microbial communities in the bioavailability of iron in Doñana National Park. Environ Geochem Health 30: 165–170.
  • 11. Rho M, Tang H, Ye Y (2010) FragGeneScan: predicting genes in short and error-prone reads. Nucl Acids Res 38: e191.    
  • 12. Thompson JD, Higgins DG, Gibson TJ (1994) ClustalW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl Acids Res 22: 4673–4680.
  • 13. Zhu Y, Liu G, Li H, et al. (2012) Cloning and characterization of a thermostable carboxylesterase from inshore hot spring thermophile Geobacillussp. ZH1. Acta Oceanol Sin 31: 117–126.
  • 14. Asoodeh A, Ghanbari T (2013) Characterization of an extracellular thermophilic alkaline esterase produced by Bacillus subtilis DR8806. J Mol Catal B 85–86: 49–55.
  • 15. Brault G, Shareck F, Hurtubise Y, et al. (2012) Isolation and characterization of EstC, a new cold-active esterase from Streptomyces coelicolor A3(2). PloS One 7(3): e32041.
  • 16. Mohamed YM, Ghazy MA, Sayed A, et al. (2013) Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Pool. Scientific Rep 3: 3358–3358.
  • 17. Andrade JP, da Rocha Bispo AS, Marbach PAS, et al. (2011) Production and partial characterization of cellulases from Trichoderma sp. IS-05 isolated from sandy coastal plains of Northeast Brazil. Enz Res 167248: 1–7.
  • 18. Ling L, Kan X, Hao Y, et al. (2012) Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains. Electronic J Biotechnol 15: 717–3458.
  • 19. Buchholz K, Kasche V, Bornscheuer UT (2012) Biocatalysts and enzyme technology, 2 Eds., Hoboken, New Jersey: Wiley-Blackwell.
  • 20. Madigan MT, Martinko JM, Parker J (2003) Brock Biology of microorganisms. New Jersey: Prentice-Hall Inc.
  • 21. De Azeredo LAI, Freire DMG, Soares RMA, et al. (2004) Production and partial characterization of thermophilic proteases from Streptomyces sp. Isolated from Brazilian Cerrado soil. Enz Microb Technol 34: 354–358.
  • 22. Jayakumar R, Jayashree S, Annapurna B, et al. (2012) Characterization of thermophilic serine alkaline protease from an alkaliphilic strain Bacillus pumilus MCAS8 and its applications. Appl Biochem Biotechnol 168: 1849–1866.
  • 23. Deb P, Talukdar SA, Mohsina K, et al. (2013) Production and partial characterization of extracellular amylase enzyme from Bacillus amyloliquefaciens P-001. SpringerPlus 2: 1–12.
  • 24. Kim DW, Jang YH, Kim CS, et al. (2011) Effect of metal ions on the degradation and adsorption of two cellobiohydrolases on microcrystalline cellulose. Bull Korean Chem Soc 22: 716–720.
  • 25. Shanker AK (2008) Mode of action and toxicity of trace metals. In: Prasad MNV, editor, Trace elements as contaminants and nutrients: consequences in ecosystems and human health. Hoboken, New Jersey: John Wiley & Sons Inc.
  • 26. Kresse R, Baudis U, Jäger P, et al. (2007) Barium and barium compounds. Ullmann’s Encyclopedia of Industrial Chemistry. Boca Raton, Florida: CRC Press.


This article has been cited by

  • 1. Adriana F. M. Braga, Maria Beatriz O. C. Pereira, Marcelo Zaiat, Gustavo H. R. da Silva, Fernando G. Fermoso, Screening of trace metal supplementation for black water anaerobic digestion, Environmental Technology, 2017, 1, 10.1080/09593330.2017.1340343
  • 2. Bhagwan Rekadwad, Juan M. Gonzalez, Multidisciplinary involvement and potential of thermophiles, Folia Microbiologica, 2018, 10.1007/s12223-018-0662-8
  • 3. V. Wyman, A. Serrano, R. Borja, A. Jiménez, A. Carvajal, M. Lenz, J. Bartacek, F.G. Fermoso, Effects of barium on the pathways of anaerobic digestion, Journal of Environmental Management, 2019, 232, 397, 10.1016/j.jenvman.2018.11.065

Reader Comments

your name: *   your email: *  

Copyright Info: 2016, Juan M. Gonzalez, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

Download full text in PDF

Export Citation

Copyright © AIMS Press All Rights Reserved