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Hydrolytic enzyme activity enhanced by Barium supplementation

1 Center for Technological and Scientific Research in Mining (CICITEM) and Biotechnology Department, University of Antofagasta, Avda. José Miguel Carrera 1701, Antofagasta, Chile
2 Institute La Grasa, Spanish National Research Council (IG-CSIC), Avda. Padre García Tejero 4, 41012 Sevilla, Spain
3 Institute of Natural Resources and Agrobiology, Spanish National Research Council (IRNAS-CSIC), Avda. Reina Mercedes 10, 41012 Sevilla, Spain

Topical Section: Microorganisms in sustainable agriculture and biotechnology

Hydrolysis of polymers is a first and often limiting step during the degradation of plant residues. Plant biomass is generally a major component of waste residues and a major renewable resource to obtain a variety of secondary products including biofuels. Improving the performance of enzymatic hydrolysis of plant material with minimum costs and limiting the use of additional microbial biomass or hydrolytic enzymes directly influences competitiveness of these green biotechnological processes. In this study, we cloned and expressed a cellulase and two esterases recovered from environmental thermophilic soil bacterial communities and characterize their optimum activity conditions including the effect of several metal ions. Results showed that supplementing these hydrolytic reactions with Barium increases the activity of these extracellular hydrolytic enzymes. This observation represents a simple but major improvement to enhance the efficiency and competitiveness of this process within an increasingly important biotechnological sector.
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